LOCUS LT727567 6360 bp DNA circular SYN 06-FEB-2017
DEFINITION Vector pURgHIL22, complete sequence.
ACCESSION LT727567
VERSION LT727567.1
KEYWORDS .
SOURCE Vector pURgHIL22
ORGANISM Vector pURgHIL22
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 6360)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..6360
/organism="Vector pURgHIL22"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 1012."
/db_xref="taxon:1945438"
regulatory 372..455
/note="lac"
/regulatory_class="promoter"
intron 541..1528
/note="intron of hIL22"
exon 1527..1672
/note="exon3 of hIL22"
intron 1673..3517
/note="intron of hIL22"
exon 3518..3628
/note="exon of hIL22"
misc_feature 3629..3904
/note="3' UTR of hIL22"
rep_origin complement(4357..4760)
/note="pMB1 ori"
CDS complement(5300..6160)
/transl_table=11
/note="ampicillin resistance"
/protein_id="SJL88863.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRXXAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
BASE COUNT 1887 a 1270 c 1253 g 1944 t
ORIGIN
1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
121 ttggcgggtg tcggggcgca gccatgaccc agtcacgtag cgatagcgga gtgtatatac
181 tggcttaact atgcggcatc agagcagatt gtactgagag tgcaccatat gcggtgtgaa
241 ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc gcccaatacg caaaccgcct
301 ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa
361 gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct
421 ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac
481 acaggaaaca gctatgacca tgattacgcc aagctttcta gagtcgacgg atccggggaa
541 ttcatagcca caccaaatct atgaagagtt tcaagagtgt ttgtgtttgg cattttaaga
601 agaaggaaaa tataatctgc taagagatgc cagaactaag ggtctgatca gcccttgaaa
661 ggacaaagca agggcttttg agaagtctac atctctgttc tacatatttc ttcagagaaa
721 gagtgacttt tgtaagcatt ttaaccatcc tacagttcac acaagtaact aagaaatatt
781 taaatgctta ctctgtgctg tttccaaaat aacaaatatc caggcttgtt tatatttatc
841 aaatttatta aatagtttta ctaaccaatc tacatagcac tatatttaac attcaacata
901 ataataaata ttttgggata aataagtgaa accattttag agcccctagg gcacaaaaag
961 aatcataaaa gatccatatt tatagtttta agattaagaa taatagttac aataggtagc
1021 aaaccataca ttcaacaata aatataaaat ttaaatattt aaataaatag aaggcctgat
1081 atgttttaag tgggaagcac ttaattatca agtcagtgtt gagatgatgc tttgacaaaa
1141 ggtaatccat ctgttcagaa attctacaat ggttgctgtc tcatcagcat attcacacat
1201 gaatgttgtt tcagatccct ataaaagaaa aatgttaatt ttttaaagta cagagtagtt
1261 taccttatat acagttattc ccaattgaag tcttataggc ctgttgcctt tattttcaag
1321 cttaccaaac atattaatta ttcgcatttt cttgaaatga tcaaaatgaa tgccaaaatt
1381 acatattttg ttatgatacc aaatgatagt aacacagaag ctgagatttt cctcatttgt
1441 taattcagtc aatgaacaca aaaatttttt ccctaaattg ggtgcaaata aagatttaaa
1501 taaaaataga tctagacaaa tattaaatgc tggatgtgaa ataaacttga tcgcttttcc
1561 tctgaatgta cacctatatt tgtgtaagtt aaatgtatga attgatatgc acattatact
1621 ttcttctttt ctaaatcttg attagagtct cctatttttc tacttaagtg aatctcctta
1681 tattttcccc aaagcagaac agaactacac tagagtaagc taggacatgc ttccgtttgg
1741 gataaatttg tgtgaagaac atcttctaaa tacttacacc tatccctacc ccatcatagt
1801 atcaatgcag gtgaatttgc caacttttaa aagtttaaac attagaaatt ttgttctgtg
1861 gactggcttt ttagaaatta ggcttctctg ctgaaaggag ctatttaaac gcttcctcta
1921 gttgataagc taaggaggaa atattttgat atatagtgag atttaaaaga gagcttttca
1981 aatttgaagt gattcctacc ctgggaacaa aagcaggtga gatagagtgt tccctttaaa
2041 taagtattct cttttaaaaa actgttgcag tgttctgtat cctcttaagc ataattgttt
2101 aaatattgtc atatcttggg attttttatt ttaaaaagtg tttggctttt gaaacacagt
2161 tttcagattt ttgcatggtt ttgtcttaaa tttgtatcaa gagttaagaa tgagttttct
2221 gatgttaatt attttagagt aaaactcttg ccatagataa ggaactgaat ctgtcaatgt
2281 ctagatgaca ttgatctcaa tgtatttttt taattcaact gaaacagtcc actaataagt
2341 gattcatttg gacacaactg gagttacttt taggaatgtc ttttactact atacttatgc
2401 tgcttattta ggatacctat taactcatga attttatacc ttaggagacg ggatataaaa
2461 agacaaaagt gaatctcttt tacatcacct gagtcccttg catattttat gcttcctgtg
2521 catttaaaaa tacttaagat tctgaaagtg gggattgcct ctaaaaagat tgttgtttgt
2581 ttagtccact ggccatttta atggctccag tttttttttt taagacaaat ctcttatggg
2641 tagtccttcc atcaaatgat aaggaaacac catttgaaaa gcctttcagg taatcagtat
2701 tggctatttc agtaaatgat ctgaagaatc gaaaaatata taataaaatc tgcctgcttt
2761 ctgtgaaact caactcagta ccttctctca ctcccagttt tgtgttaata tgaaaggtga
2821 tggagtttgg gatgaggctt gtccattagt agatggtagg tgcaaactca gtagcttata
2881 ctccccagtt aatcatttaa cattagaaat gattcatgtt cacagtgtac ttaaaatgta
2941 ggctaattac atgcatgggt actttacaaa taccaaaaat gttatgtcac tttaaaatgt
3001 ggtacttttc cccctgactt tttttttttt atttccagga gagcaaataa agtaatgcct
3061 tacctttagt tccagaacta ttacgttgat attgctgatt aagtccctgg gtcttaagtg
3121 aaagtttttg ctttgagcta aatttagcac ttcctccaga ggtttgagtt cttcttctag
3181 acactgaaga tgtttcagtt ctgtggccta gaataataat tatcatcagc tcagtttaca
3241 tagaggtcag aatcagaaat taatttccaa tttattttat ttggagtagc agtatgtagt
3301 ttttaccatt ctctctgttc tcaatgtcac tgtaaagaat ggaccagtag agtttacatt
3361 ataaaactgg aaaaactagg gctcaagtac ctatgactag cgttaagtga tagttgactc
3421 actggtttta gcttaatttg gctttgcaaa atgttagact ttctgcatcg taagtgccta
3481 tcacagatgc aggctgatta cttttccaat ctgcatagaa aatcattaga accagatgag
3541 ttagaatgtg gaagcataaa aatcacattc tatgattata atatttgata gtctaagagt
3601 taagaatcac agaatgttga gagagaagga actcttgaat gccatctatc acaatcccaa
3661 cattttacac taggtaacaa taatcatgta gtagtggctc tcaatatagc cttgagcaag
3721 acacattttt aaatatgtct taaattttac ttttctacaa tcactacatt aagatgcatt
3781 ttaaaaatgt ttgtcccagt gaaataaaag attataatac tttgggagga cttttggaca
3841 ggcctcttga ttctatatta tatataatat ttcatatttc tatggtatat tacaatttaa
3901 aagaattttc tccaaaatag gcccatttac ttctcacagt catcttttga agtaggcagt
3961 gaagtatcat ctccacttta taaataagca cactaaatct cagaatttga ccacataagt
4021 aggtagagcc agaattagat ttcatttctt tctcattata gcattaacat agcatccttg
4081 gaatcaaata tttttattag tttctaaaat cttttgtaaa tgtgcaatga gggttaaatg
4141 aacaaatgaa tagagtttat cattactttt ggtttcaaac ttagatttcc atgaattcac
4201 tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc
4261 ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc
4321 cttcccaaca gttgcgcagc ctgaatggcg aatggcgctc ttccgcttcc tcgctcactg
4381 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
4441 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
4501 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
4561 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
4621 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
4681 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
4741 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
4801 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
4861 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
4921 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
4981 ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
5041 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
5101 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
5161 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
5221 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
5281 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
5341 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
5401 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
5461 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
5521 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
5581 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
5641 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
5701 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
5761 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
5821 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
5881 gtatgcggcg accgagttgc tcttgcccgg cnnnnnnacg ggataatacc gcgccacata
5941 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
6001 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
6061 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
6121 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
6181 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
6241 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag
6301 aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc
//