LOCUS LT727566 7301 bp DNA circular SYN 06-FEB-2017
DEFINITION Vector BH20, complete sequence.
ACCESSION LT727566
VERSION LT727566.1
KEYWORDS .
SOURCE Vector BH20
ORGANISM Vector BH20
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 7301)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..7301
/organism="Vector BH20"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 7765."
/db_xref="taxon:1945236"
regulatory complement(26..73)
/note="T7 terminator"
/regulatory_class="terminator"
misc_feature complement(140..157)
/note="His-tag"
misc_feature complement(179..356)
/note="RT-PCR positive control DNA"
CDS complement(406..798)
/transl_table=11
/note="COAT"
/protein_id="SJL88858.1"
/translation="MASNFTQFVLVDNGGTGDVTVAPSNFANGVAEWISSNSRSQAYK
VTCSVRQSSAQNRKYTIKVEVPKVATQTVGGVELPVAAWRSYLNMELTIPIFATNSDC
ELIVKAMQGLLKDGNPIPSAIAANSGIY"
CDS complement(822..2003)
/transl_table=11
/note="A"
/protein_id="SJL88859.1"
/translation="MRAFSTLDRENETFVPSVRVYADGETEDNSFSLKYRSNWTPGRF
NSTGAKTKQWHYPSPYSRGALSVTSIDQGAYKRSGSSWGRPYEEKAGFGFSLDARSCY
SLFPVSQNLTYIEVPQNVANRASTEVLQKVTQGNFNLGVALAEARSTASQLATQTIAL
VKAYTAARRGNWRQALRYLALNEDRKFRSKHVAGRWLELQFGWLPLMSDIQGAYEMLT
KVHLQEFLPMRAVRQVGTNIKLDGRLSYPAANFQTTCNISRRIVIWFYINDARLAWLS
SLGILNPLGIVWEKVPFSFVVDWLLPVGNMLEGLTAPVGCSYMSGTVTDVITGESIIS
VDAPYGWTVERQGTAKAQISAMHRGVQSVWPTTGAYVKSPFSMVHTLDALALIRQRLS
R"
misc_feature complement(2128..2172)
/note="S-tag"
misc_feature complement(2179..2196)
/note="thrombin recognition site"
misc_feature complement(2206..2223)
/note="His-tag"
misc_feature complement(2227..2271)
/note="5' UTR of T7 gene 10, incl. RBS"
regulatory complement(2273..2314)
/note="T7 promoter with lac operator"
/regulatory_class="promoter"
regulatory 2627..2704
/note="lacI promoter, incl. RBS"
/regulatory_class="promoter"
CDS 2705..3787
/transl_table=11
/note="lacI"
/protein_id="SJL88860.1"
/translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAE
LNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERS
GVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSII
FSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREG
DWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDT
EDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNT
QTASPRALADSLMQLARQVSRLESGQ"
CDS 4596..4787
/transl_table=11
/note="rop"
/protein_id="SJL88861.1"
/translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDH
ADELYRSCLARFGDDGENL"
rep_origin complement(5033..5435)
/note="pMB1 ori"
CDS 5927..6742
/transl_table=11
/note="Tn903 neomycin resistance"
/protein_id="SJL88862.1"
/translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLY
GKPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIP
GKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGL
VDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVG
RVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
rep_origin complement(6835..7290)
/note="f1 ori"
BASE COUNT 1749 a 1873 c 1942 g 1737 t
ORIGIN
1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa
61 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt
121 tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagtgcggcc gcaagctttc
181 tgcaaaaaca tcttcaagtc tctgagctgt tgcaacccca agcctgagga gaggcatttg
241 ctaagatcta ctagaaggca gggatgatgt tctggagagc cctctattgc acttgccccg
301 tagctggatg tcctcacctg catacgcgtt cgacctcggt tagaagactc atctccaagc
361 tttcttcgac atgggtaatc ctcatgtttg aatggccggc gtctattagt agatgccgga
421 gtttgctgcg attgctgagg gaatcgggtt tccatctttt aggagacctt gcattgcctt
481 aacaataagc tcgcagtcgg aattcgtggc gaaaattgga atggttagtt ccatatttaa
541 gtacgaacgc catgcggcta caggaagctc tacaccacca acagtctggg ttgccacttt
601 aggcacctcg actttgatgg tgtatttgcg attctgcgca gagctctgac gaacgctaca
661 ggttactttg taagcctgtg aacgcgagtt agagctgatc cattcagcga ccccgttagc
721 gaagttgctt ggggcgacag tcacgtcgcc agttccgcca ttgtcgacga gaacgaactg
781 agtaaagtta gaagccatgc ttcaaactcc ggttgagggc tctatctaga gagccgttgc
841 ctgattaatg ctaacgcatc taaggtatgg accatcgaga aaggagactt tacgtacgcg
901 ccagttgttg gccatacgga ttgtacccct cgatgcatgg ctgagatttg ggccttagca
961 gtgccctgtc tctccacagt ccacccgtag ggagcgtcaa cgcttatgat ggactcaccc
1021 gttattacgt cagtaactgt tcctgacatg taggagcatc ccacgggggc cgtaaggccc
1081 tcgagcatgt tacctacagg taggagccag tcgacaacga atgagaaagg caccttttcc
1141 cacactatac ctagtgggtt caagatacct agagacgaca accatgccaa acgtgcatcg
1201 tttatgtaaa accatatcac gatacgtcgc gatatgttgc acgttgtctg gaagtttgca
1261 gctggatacg acagacggcc atctaacttg atgttagtac cgacctgacg tacggctctc
1321 ataggaagaa actcttgaag gtgaaccttc gtaagcatct catatgcacc ctggatatca
1381 ctcattagtg gtaaccaacc gaactgcaac tccaaccacc tgccggccac gtgttttgat
1441 cgaaactttc gatcttcgtt tagggcaagg tagcggagcg cctggcgcca attaccgcga
1501 cgagcggcag tgtacgcctt cacgagcgca atggtttgcg tcgcgagttg tgaggctgtc
1561 gacctggcct ctgctaaagc aacaccaagg ttaaaattac cctgggtgac cttttgcagg
1621 acttcggtcg acgcccggtt cgcaacgttc tgcggcactt cgatgtaagt caagttttgg
1681 cttacaggga agaggctgta gcaggagcgt gcgtcgaggg agaagccgaa accggctttc
1741 tcctcgtacg ggcgacccca cgatgaccca cttcgcttgt aggcaccttg atctatcgat
1801 gtgacactta acgccccccg tgaatacgga gaggggtagt gccactgttt cgttttggcc
1861 ccagtcgagt taaaacgacc gggagtccag ttcgaacgat attttaaaga gaatgagtta
1921 tcttcagtct caccgtccgc gtaaacgcga acggagggga cgaaggtctc gttctcccta
1981 tcaagggtac taaaagctcg cacaggtcaa acctcctagg aatggaattc cggctaccta
2041 cagcgatagc catggtagcg tctcgctaaa gacattaaaa atggcattag ctcgacagga
2101 agttgagcag gaggtaccca gatctgggct gtccatgtgc tggcgttcga atttagcagc
2161 agcggtttct ttcataccag aaccgcgtgg caccagacca gaagaatgat gatgatgatg
2221 gtgcatatgt atatctcctt cttaaagtta aacaaaatta tttctagagg ggaattgtta
2281 tccgctcaca attcccctat agtgagtcgt attaatttcg cgggatcgag atcgatctcg
2341 atcctctacg ccggacgcat cgtggccggc atcaccggcg ccacaggtgc ggttgctggc
2401 gcctatatcg ccgacatcac cgatggggaa gatcgggctc gccacttcgg gctcatgagc
2461 gcttgtttcg gcgtgggtat ggtggcaggc cccgtggccg ggggactgtt gggcgccatc
2521 tccttgcatg caccattcct tgcggcggcg gtgctcaacg gcctcaacct actactgggc
2581 tgcttcctaa tgcaggagtc gcataaggga gagcgtcgag atcccggaca ccatcgaatg
2641 gcgcaaaacc tttcgcggta tggcatgata gcgcccggaa gagagtcaat tcagggtggt
2701 gaatgtgaaa ccagtaacgt tatacgatgt cgcagagtat gccggtgtct cttatcagac
2761 cgtttcccgc gtggtgaacc aggccagcca cgtttctgcg aaaacgcggg aaaaagtgga
2821 agcggcgatg gcggagctga attacattcc caaccgcgtg gcacaacaac tggcgggcaa
2881 acagtcgttg ctgattggcg ttgccacctc cagtctggcc ctgcacgcgc cgtcgcaaat
2941 tgtcgcggcg attaaatctc gcgccgatca actgggtgcc agcgtggtgg tgtcgatggt
3001 agaacgaagc ggcgtcgaag cctgtaaagc ggcggtgcac aatcttctcg cgcaacgcgt
3061 cagtgggctg atcattaact atccgctgga tgaccaggat gccattgctg tggaagctgc
3121 ctgcactaat gttccggcgt tatttcttga tgtctctgac cagacaccca tcaacagtat
3181 tattttctcc catgaagacg gtacgcgact gggcgtggag catctggtcg cattgggtca
3241 ccagcaaatc gcgctgttag cgggcccatt aagttctgtc tcggcgcgtc tgcgtctggc
3301 tggctggcat aaatatctca ctcgcaatca aattcagccg atagcggaac gggaaggcga
3361 ctggagtgcc atgtccggtt ttcaacaaac catgcaaatg ctgaatgagg gcatcgttcc
3421 cactgcgatg ctggttgcca acgatcagat ggcgctgggc gcaatgcgcg ccattaccga
3481 gtccgggctg cgcgttggtg cggacatctc ggtagtggga tacgacgata ccgaagacag
3541 ctcatgttat atcccgccgt taaccaccat caaacaggat tttcgcctgc tggggcaaac
3601 cagcgtggac cgcttgctgc aactctctca gggccaggcg gtgaagggca atcagctgtt
3661 gcccgtctca ctggtgaaaa gaaaaaccac cctggcgccc aatacgcaaa ccgcctctcc
3721 ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg
3781 gcagtgagcg caacgcaatt aatgtaagtt agctcactca ttaggcaccg ggatctcgac
3841 cgatgccctt gagagccttc aacccagtca gctccttccg gtgggcgcgg ggcatgacta
3901 tcgtcgccgc acttatgact gtcttcttta tcatgcaact cgtaggacag gtgccggcag
3961 cgctctgggt cattttcggc gaggaccgct ttcgctggag cgcgacgatg atcggcctgt
4021 cgcttgcggt attcggaatc ttgcacgccc tcgctcaagc cttcgtcact ggtcccgcca
4081 ccaaacgttt cggcgagaag caggccatta tcgccggcat ggcggcccca cgggtgcgca
4141 tgatcgtgct cctgtcgttg aggacccggc taggctggcg gggttgcctt actggttagc
4201 agaatgaatc accgatacgc gagcgaacgt gaagcgactg ctgctgcaaa acgtctgcga
4261 cctgagcaac aacatgaatg gtcttcggtt tccgtgtttc gtaaagtctg gaaacgcgga
4321 agtcagcgcc ctgcaccatt atgttccgga tctgcatcgc aggatgctgc tggctaccct
4381 gtggaacacc tacatctgta ttaacgaagc gctggcattg accctgagtg atttttctct
4441 ggtcccgccg catccatacc gccagttgtt taccctcaca acgttccagt aaccgggcat
4501 gttcatcatc agtaacccgt atcgtgagca tcctctctcg tttcatcggt atcattaccc
4561 ccatgaacag aaatccccct tacacggagg catcagtgac caaacaggaa aaaaccgccc
4621 ttaacatggc ccgctttatc agaagccaga cattaacgct tctggagaaa ctcaacgagc
4681 tggacgcgga tgaacaggca gacatctgtg aatcgcttca cgaccacgct gatgagcttt
4741 accgcagctg cctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc
4801 cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg
4861 cgtcagcggg tgttggcggg tgtcggggcg cagccatgac ccagtcacgt agcgatagcg
4921 gagtgtatac tggcttaact atgcggcatc agagcagatt gtactgagag tgcaccatat
4981 atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag gcgctcttcc
5041 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
5101 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
5161 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
5221 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
5281 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
5341 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
5401 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
5461 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
5521 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
5581 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
5641 tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc
5701 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
5761 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
5821 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
5881 aacaataaaa ctgtctgctt acataaacag taatacaagg ggtgttatga gccatattca
5941 acgggaaacg tcttgctcta ggccgcgatt aaattccaac atggatgctg atttatatgg
6001 gtataaatgg gctcgcgata atgtcgggca atcaggtgcg acaatctatc gattgtatgg
6061 gaagcccgat gcgccagagt tgtttctgaa acatggcaaa ggtagcgttg ccaatgatgt
6121 tacagatgag atggtcagac taaactggct gacggaattt atgcctcttc cgaccatcaa
6181 gcattttatc cgtactcctg atgatgcatg gttactcacc actgcgatcc ccgggaaaac
6241 agcattccag gtattagaag aatatcctga ttcaggtgaa aatattgttg atgcgctggc
6301 agtgttcctg cgccggttgc attcgattcc tgtttgtaat tgtcctttta acagcgatcg
6361 cgtatttcgt ctcgctcagg cgcaatcacg aatgaataac ggtttggttg atgcgagtga
6421 ttttgatgac gagcgtaatg gctggcctgt tgaacaagtc tggaaagaaa tgcataaact
6481 tttgccattc tcaccggatt cagtcgtcac tcatggtgat ttctcacttg ataaccttat
6541 ttttgacgag gggaaattaa taggttgtat tgatgttgga cgagtcggaa tcgcagaccg
6601 ataccaggat cttgccatcc tatggaactg cctcggtgag ttttctcctt cattacagaa
6661 acggcttttt caaaaatatg gtattgataa tcctgatatg aataaattgc agtttcattt
6721 gatgctcgat gagtttttct aagaattaat tcatgagcgg atacatattt gaatgtattt
6781 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgaaattg
6841 taaacgttaa tattttgtta aaattcgcgt taaatttttg ttaaatcagc tcatttttta
6901 accaataggc cgaaatcggc aaaatccctt ataaatcaaa agaatagacc gagatagggt
6961 tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac tccaacgtca
7021 aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca ccctaatcaa
7081 gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg agcccccgat
7141 ttagagcttg acggggaaag ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag
7201 gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc accacacccg
7261 ccgcgcttaa tgcgccgcta cagggcgcgt cccattcgcc a
//