LOCUS LT727564 3455 bp DNA circular SYN 06-FEB-2017
DEFINITION Vector pMCQ, complete sequence.
ACCESSION LT727564
VERSION LT727564.1
KEYWORDS .
SOURCE Vector pMCQ
ORGANISM Vector pMCQ
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 3455)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..3455
/organism="Vector pMCQ"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 2977."
/db_xref="taxon:1945348"
rep_origin complement(3..458)
/note="f1 ori"
regulatory 626..642
/note="T7 promoter"
/regulatory_class="promoter"
misc_feature 708..732
/note="TNF"
misc_feature 733..757
/note="IL2 fragment"
misc_feature 758..782
/note="IL5 fragment"
misc_feature 783..807
/note="IL4 fragment"
misc_feature 808..832
/note="IL3 fragment"
misc_feature 833..857
/note="IL6 fragment"
misc_feature 858..880
/note="IL1a fragment"
misc_feature 881..905
/note="LT fragment"
misc_feature 906..931
/note="IFNG fragment"
misc_feature 932..956
/note="B-ACTIN fragment"
misc_feature complement(979..999)
/note="IL1a fragment"
misc_feature complement(1000..1024)
/note="IL2 fragment"
misc_feature complement(1025..1048)
/note="IL3 fragment"
misc_feature complement(1049..1073)
/note="IL4 fragment"
misc_feature complement(1074..1098)
/note="IL5 fragment"
misc_feature complement(1099..1123)
/note="IL6 fragment"
misc_feature complement(1124..1148)
/note="TNF fragment"
misc_feature complement(1149..1173)
/note="LT fragment"
misc_feature complement(1174..1198)
/note="B-ACTIN fragment"
misc_feature complement(1199..1222)
/note="IFNG fragment"
misc_feature complement(1223..1248)
/note="polyA tail"
regulatory complement(1269..1285)
/note="T3 promoter"
/regulatory_class="promoter"
misc_feature complement(1311..1348)
/note="5UTR"
regulatory complement(1349..1432)
/note="lac"
/regulatory_class="promoter"
rep_origin complement(1525..1925)
/note="pMB1 ori"
CDS complement(2467..3327)
/transl_table=11
/note="ampicillin resistance"
/protein_id="SJL88852.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
BASE COUNT 892 a 851 c 884 g 828 t
ORIGIN
1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tggagctcca
661 ccgcggtggc ggccgctcta gaactagtgg atcccccggg ctgcagcggc aggtctactt
721 tggagtcatt gctgatggac ctacaggagc tcctgagatg actgtgcctc tgtgcctgga
781 gccgaagaac accacagaga gtgagctgaa gtggatcctg aggacagata cgtggagtca
841 cagaaggagt ggctaagctc tagagcacca tgctacagac tggctggcaa caggggaagg
901 ttgacagcgg ctgactgaac tcagattgta gtggaatcct gtggcatcca tgaaacaagc
961 ttatcgatac cgtcgaccca gtgtttcccc tggattccac tgcggcatgt tctggatttg
1021 actcgaatgt tcctcatggc ccatggtccg ataagctgca ccatgaatga gtcccccagt
1081 ttactccagg aaaaacaggt ggacattcct cactgtggtc agaccgaatt cactggagcc
1141 tcgaatgtcc aaggggttct agaagaaagc acgcggactg ttactgagct gcgttttacc
1201 ctatacagct gaaaactgtg acaaaaaaaa aaaaaaaaaa aaaaaaaggg tacccagctt
1261 ttgttccctt tagtgagggt taattgcgcg cttggcgtaa tcatggtcat agctgtttcc
1321 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
1381 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
1441 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg
1501 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
1561 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
1621 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
1681 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
1741 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
1801 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
1861 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
1921 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
1981 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
2041 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
2101 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg
2161 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
2221 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
2281 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
2341 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
2401 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
2461 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
2521 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
2581 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
2641 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
2701 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
2761 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
2821 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
2881 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
2941 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
3001 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
3061 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
3121 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
3181 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
3241 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
3301 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
3361 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
3421 aggggttccg cgcacatttc cccgaaaagt gccac
//