LOCUS LT727559 5241 bp DNA circular SYN 06-FEB-2017 DEFINITION Bacterial expression vector pPLaA17, complete sequence. ACCESSION LT727559 VERSION LT727559.1 KEYWORDS . SOURCE Bacterial expression vector pPLaA17 ORGANISM Bacterial expression vector pPLaA17 other sequences; artificial sequences; vectors. REFERENCE 1 (bases 1 to 5241) AUTHORS De Schamphelaire W. JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent, Technologiepark 927, 9052, BELGIUM. REFERENCE 2 AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K., Roggeman Fonseca M., Vanhoucke M., Beyaert R. TITLE BCCM/LMBP Plasmid collection JOURNAL Unpublished. FEATURES Location/Qualifiers source 1..5241 /organism="Bacterial expression vector pPLaA17" /mol_type="other DNA" /note="BCCM/LMBP Plasmid collection (Ghent University,Belgium) accession number LMBP 295." /db_xref="taxon:1944689" regulatory complement(117..247) /note="PL promoter" /regulatory_class="promoter" CDS 615..1430 /transl_table=11 /note="Tn903 neomycin resistance" /protein_id="SJL88836.1" /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLY GKPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIP GKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGL VDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVG RVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" rep_origin complement(1716..2107) /note="ColE1 ori" CDS complement(2648..3508) /transl_table=11 /note="ampicillin resistance" /protein_id="SJL88837.1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" misc_feature 3717..3740 /note="5' UTR of A" CDS 3741..4922 /transl_table=11 /note="A" /protein_id="SJL88838.1" /translation="MRAFSTLDRENETFVPSVRVYADGETEDNSFSLKYRSNWTPGRF NSTGAKTKQWHYPSPYSRGALSVTSIDQGAYKRSGSSWGRPYEEKAGFGFSLDARSCY SLFPVSQNLTYIEVPQNVANRASTEVLQKVTQGNFNLGVALAEARSTASQLATQTIAL VKAYTAARRGNWRQALRYLALNEDRKFRSKHVAGRWLELQFGWLPLMSDIQGAYEMLT KVHLQEFLPMRAVRQVGTNIKLDGRLSYPAANFQTTCNISRRIVIWFYINDARLAWLS SLGILNPLGIVWEKVPFSFVVDWLLPVGNMLEGLTAPVGCSYMSGTVTDVITGESIIS VDAPYGWTVERQGTAKAQISAMHRGVQSVWPTTGAYVKSPFSMVHTLDALALIRQRLS R" misc_feature 4923..4945 /note="5' UTR of COAT" CDS 4946..>5241 /transl_table=11 /note="COATf" /protein_id="SJL88839.1" /translation="MASNFTQFVLVDNGGTGDVTVAPSNFANGVAEWISSNSRSQAYK VTCSVRQSSAQNRKYTIKVEVPKVATQTVGGVELPVAAWRSYLNMELTIPIFAT" BASE COUNT 1317 a 1273 c 1271 g 1380 t ORIGIN 1 ttccaatgct tcgtttcgta tcacacaccc caaagccttc tgctttgaat gctgcccttc 61 ttcagggctt aattttaaga gcgtcacctt catggtggtc agtgcgtcct gctgatgtgc 121 tcagtatcac cgccagtggt atttatgtca acaccgccag agataattta tcaccgcaga 181 tggttatctg tatgtttttt atatgaattt attttttgca ggggggcatt gtttggtagg 241 tgagagatcc tctacgccgg acgcatcgtg gccggcatca ccggcgctga ggtctgcctc 301 gtgaagaagg tgttgctgac tcataccagg cctgaatcgc cccatcatcc agccagaaag 361 tgagggagcc acggttgatg agagctttgt tgtaggtgga ccagttggtg attttgaact 421 tttgctttgc cacggaacgg tctgcgttgt cgggaagatg cgtgatctga tccttcaact 481 cagcaaaagt tcgatttatt caacaaagcc acgttgtgtc tcaaaatctc tgatgttaca 541 ttgcacaaga taaaaatata tcatcatgaa caataaaact gtctgcttac ataaacagta 601 atacaagggg tgttatgagc catattcaac gggaaacgtc ttgctcgagg ccgcgattaa 661 attccaacat ggatgctgat ttatatgggt ataaatgggc tcgcgataat gtcgggcaat 721 caggtgcgac aatctatcga ttgtatggga agcccgatgc gccagagttg tttctgaaac 781 atggcaaagg tagcgttgcc aatgatgtta cagatgagat ggtcagacta aactggctga 841 cggaatttat gcctcttccg accatcaagc attttatccg tactcctgat gatgcatggt 901 tactcaccac tgcgatcccc gggaaaacag cattccaggt attagaagaa tatcctgatt 961 caggtgaaaa tattgttgat gcgctggcag tgttcctgcg ccggttgcat tcgattcctg 1021 tttgtaattg tccttttaac agcgatcgcg tatttcgtct cgctcaggcg caatcacgaa 1081 tgaataacgg tttggttgat gcgagtgatt ttgatgacga gcgtaatggc tggcctgttg 1141 aacaagtctg gaaagaaatg cataagcttt tgccattctc accggattca gtcgtcactc 1201 atggtgattt ctcacttgat aaccttattt ttgacgaggg gaaattaata ggttgtattg 1261 atgttggacg agtcggaatc gcagaccgat accaggatct tgccatccta tggaactgcc 1321 tcggtgagtt ttctccttca ttacagaaac ggctttttca aaaatatggt attgataatc 1381 ctgatatgaa taaattgcag tttcatttga tgctcgatga gtttttctaa tcagaattgg 1441 ttaattggtt gtaacactgg cagagcatta cgctgacttg acgggacggc ggctttgttg 1501 aataaatcga acttttgctg agttgaagga tcagatcacg catcttcccg acaacgcaga 1561 ccgttccgtg gcaaagcaaa agttcaaaat caccaactgg tccacctaca acaaagctct 1621 catcaaccgt ggctccctca ctttctggct ggatgatggg gcgattcagg cctggtatga 1681 gtcagcaaca ccttcttcac gaggcagacc tcagcgcttt tccgcttcct cgctcactga 1741 ctcgctacgc tcggtcgttc gactgcggcg agcggtactg actcacacaa aaacggtaac 1801 acagttatcc acagaatcag gggataaggc cggaaagaac atgtgagcaa aagaccagga 1861 acaggaagaa ggccacgtag caggcgtttt tccataggct ccgcccccct gacgagcatc 1921 acaaaaatag acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg 1981 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat 2041 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt 2101 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc 2161 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg 2221 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg 2281 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg 2341 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg 2401 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca 2461 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga 2521 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga 2581 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt 2641 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt 2701 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat 2761 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag 2821 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct 2881 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt 2941 tgcgcaacgt tgttgccatt gctgcaggca tcgtggtgtc acgctcgtcg tttggtatgg 3001 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca 3061 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt 3121 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat 3181 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac 3241 cgagttgctc ttgcccggcg tcaacacggg ataataccgc gccacatagc agaactttaa 3301 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt 3361 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt 3421 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa 3481 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt 3541 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa 3601 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta 3661 tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtctt caagaattcc 3721 attcctagga ggtttgacct gtgcgagctt ttagtaccct tgatagggag aacgagacct 3781 tcgtcccctc cgttcgcgtt tacgcggacg gtgagactga agataactca ttctctttaa 3841 aatatcgttc gaactggact cccggtcgtt ttaactcgac tggggccaaa acgaaacagt 3901 ggcactaccc ctctccgtat tcacgggggg cgttaagtgt cacatcgata gatcaaggtg 3961 cctacaagcg aagtgggtca tcgtggggtc gcccgtacga ggagaaagcc ggtttcggct 4021 tctccctcga cgcacgctcc tgctacagcc tcttccctgt aagccaaaac ttgacttaca 4081 tcgaagtgcc gcagaacgtt gcgaaccggg cgtcgaccga agtcctgcaa aaggtcaccc 4141 agggtaattt taaccttggt gttgctttag cagaggccag gtcgacagcc tcacaactcg 4201 cgacgcaaac cattgcgctc gtgaaggcgt acactgccgc tcgtcgcggt aattggcgcc 4261 aggcgctccg ctaccttgcc ctaaacgaag atcgaaagtt tcgatcaaaa cacgtggccg 4321 gcaggtggtt ggagttgcag ttcggttggt taccactaat gagtgatatc cagggtgcat 4381 atgagatgct tacgaaggtt caccttcaag agtttcttcc tatgagagcc gtacgtcagg 4441 tcggtactaa catcaagtta gatggccgtc tgtcgtatcc agctgcaaac ttccagacaa 4501 cgtgcaacat atcgcgacgt atcgtgatat ggttttacat aaacgatgca cgtttggcat 4561 ggttgtcgtc tctaggtatc ttgaacccac taggtatagt gtgggaaaag gtgcctttct 4621 cattcgttgt cgactggctc ctacctgtag gtaacatgct cgagggcctt acggcccccg 4681 tgggatgctc ctacatgtca ggaacagtta ctgacgtaat aacgggtgag tccatcataa 4741 gcgttgacgc tccctacggg tggactgtgg agagacaggg cactgctaag gcccaaatct 4801 cagccatgca tcgaggggta caatccgtat ggccaacaac tggcgcgtac gtaaagtctc 4861 ctttctcgat ggtccatacc ttagatgcgt tagcattaat caggcaacgg ctctctagat 4921 agagccctca accggagttt gaagcatggc ttctaacttt actcagttcg ttctcgtcga 4981 caatggcgga actggcgacg tgactgtcgc cccaagcaac ttcgctaacg gggtcgctga 5041 atggatcagc tctaactcgc gttcacaggc ttacaaagta acctgtagcg ttcgtcagag 5101 ctctgcgcag aatcgcaaat acaccatcaa agtcgaggtg cctaaagtgg caacccagac 5161 tgttggtggt gtagagcttc ctgtagccgc atggcgttcg tacttaaata tggaactaac 5221 cattccaatt ttcgctacga a //