LOCUS LT727554 9962 bp DNA circular SYN 06-FEB-2017
DEFINITION Vector pSC2D, complete sequence.
ACCESSION LT727554
VERSION LT727554.1
KEYWORDS .
SOURCE Vector pSC2D
ORGANISM Vector pSC2D
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 9962)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..9962
/organism="Vector pSC2D"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 2892."
/db_xref="taxon:1945391"
rep_origin 1..838
/note="ARS1"
regulatory complement(839..1109)
/note="CYC1T"
/regulatory_class="terminator"
CDS complement(1198..1761)
/transl_table=11
/note="mDHFR"
/protein_id="SJL88823.1"
/translation="MVRPLNCIVAVSQNMGIGKNGDLPWPPLRNEFKYFQRMTTTSSV
EGKQNLVIMGRKTWFSIPEKNRPLKDRINIVLSRELKEPPRGAHFLAKSLDDALRLIE
QPELASKVDMVWIVGGSSVYQEAMNQPGHLRLFVTRIMQEFESDTFFPEIDLGKYKLL
PEYPGVLSEVQEEKGIKYKFEVYEKKD"
regulatory complement(1977..2958)
/note="CYC1"
/regulatory_class="promoter"
CDS 4596..5399
/transl_table=11
/note="URA3"
/protein_id="SJL88824.1"
/translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKEL
LELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVK
LQYSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLS
TGEYTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYR
TVDDVVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
rep_origin 6687..7667
/note="2mu ori"
rep_origin complement(7951..8353)
/note="pMB1 ori"
CDS complement(8894..9754)
/transl_table=11
/note="ampicillin resistance"
/protein_id="SJL88825.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
BASE COUNT 2681 a 2285 c 2260 g 2736 t
ORIGIN
1 ttccacatgt taaaatagtg aaggagcatg ttcggcacac agtggaccga acgtggggta
61 agtgcactag ggtccggtta aacggatctc gcattgatga ggcaacgcta attatcaaca
121 tatagattgt tatctatctg catgaacacg aaatctttac ttgacgactt gaggctgatg
181 gtgtttatgc aaagaaacca ctgtgtttaa tatgtgtcac tgtttgatat tactgtcagc
241 gtagaagata atagtaaaag cggttaataa gtgtatttga gataagtgtg ataaagtttt
301 tacagcgaaa agacgataaa tacaagaaaa tgattacgag gatacggaga gaggtatgta
361 catgtgtatt tatatactaa gctgccggcg gttgtttgca agaccgagaa aaggctagca
421 agaatcgggt cattgtagcg tatgcgcctg tgaacattct cttcaacaag tttgattcca
481 ttgcggtgaa atggtaaaag tcaaccccct gcgatgtata ttttcctgta caatcaatca
541 aaaagccaaa tgatttagca ttatctttac atcttgttat tttacagatt ttatgtttag
601 atcttttatg cttgcttttc aaaaggcctg caggcaagtg cacaaacaat acttaaataa
661 atactactca gtaataacct atttcttagc atttttgacg aaatttgcta ttttgttaga
721 gtcttttaca ccatttgtct ccacacctcc gcttacatca acaccaataa cgccatttaa
781 tctaagcgca tcaccaacat tttctggcgt cagtccacca gctaacataa aatgtaagct
841 tgcaaattaa agccttcgag cgtcccaaaa ccttctcaag caaggttttc agtataatgt
901 tacatgcgta cacgcgtctg tacagaaaaa aaagaaaaat ttgaaatata aataacgttc
961 ttaatactaa cataactata aaaaaataaa tagggaccta gacttcaggt tgtctaactc
1021 cttccttttc ggttagagcg gatgtggggg gagggcgtga atgtaagcgt gacataacta
1081 attacatgat atcgacaaag gaaaaggggg acggatctaa agccagcaaa agtcccatgg
1141 tcttataaaa atgcatagct ttaggagggg agcagagaac ttgaaagcat cttcctgtta
1201 gtctttcttc tcgtagactt caaacttata cttgatgcct ttttcctcct ggacctcaga
1261 gaggacgcct gggtattctg ggagaagttt atatttcccc aaatcaattt ctgggaaaaa
1321 cgtgtcactt tcaaattcct gcatgatcct tgtcacaaag agtctaaggt ggcctggttg
1381 attcatggct tcctggtaaa cagaactgcc tccgactatc caaaccatgt ctactttact
1441 tgccaattcc ggttgttcaa taagtcttaa ggcatcatcc aaacttttgg caagaaaatg
1501 agctcctcgt ggtggttctt tgagttctct actgagaact atattaattc tgtcctttaa
1561 aggtcgattc ttctcaggaa tggagaacca ggttttccta cccataatca ccagattctg
1621 tttaccttcc actgaagagg ttgtggtcat tctttggaag tacttgaact cgttcctgag
1681 cggaggccag ggtaggtctc cgttcttgcc aatccccata ttttgggaca cggcgacgat
1741 gcagttcaat ggtcgaacca tgatggcagc ggggataaaa tcctaccagc cttcacgcta
1801 ggattgccgt caagtttggc gcgaaatcgc agccctgagc tgtccccccc cccaagctcc
1861 ggatccccgg gaattcagtt attattaatt tagtgtgtgt atttgtgttt gcgtgtctat
1921 agaagtatag taatttatgc tacaaaggac ctaatgtata aggaaagaat atttagagaa
1981 aagaagaaaa caagagtttt atatacatac agagcacatg catgccatat gatcatgtgt
2041 cgtcgcacac atatatatat gcctgtatgt gtcagcacta aagttgcctg gccatccacg
2101 ctatatacac gcctggcgga tctgctcgag gattgcctac gcgtgggctt gatccaccaa
2161 ccaacgctcg ccaaatgagc tggcgcttgg tcttcggtca tcgtccgtaa accccggcca
2221 aagagaccgg aaagatcggt gaaaacatct tgatcttgct cccgggaatt ttagattcag
2281 gtaggaaatt gattacataa atactgttac cctgaatcat attcgacgat gtcgtctcac
2341 acggaaatat aattcatttc ttggttttcc aaaaaaattt tcattttttt tcactttttt
2401 gtttcgtcct cctttttttt tttttttatt ttttttcctg tgttcacctt tttttttttt
2461 ttttttcagt ttacaacttt ctgcattctt ttctgtgttt tttttttttt cgtttttcca
2521 tttgttcgtt cgttgcctgt ttttttgccc tattgttctc gagcctaaaa attttttcct
2581 ttcctgcttt cctttcttcg ttcaaagttt cctattccat tgttctcttc ggtaaactca
2641 ttgttgctcg gaactcagat atattcaggt caatttactg tacttcaatt gacttttttc
2701 ttgaaatttc aacttgcctt tcaacttgtt cttctttttt aattcttatt ctacacttta
2761 gttcccttac cttgttccta attattgtct gcaaaagaaa acatacacct atttcattca
2821 cacactaaaa catgtctgac gaagaacaca acctttgaaa atgctgacgc tggtgcctcc
2881 gccacctacc caatgcaatg ttctgccttg agaaagaatg gtttcgttgt catcaaaggt
2941 agaccatgta agattgtcga catgtccact tccaagaccg gtaagcacgg tcacgccaag
3001 gtccatttgg ttacccttga tatcttcact ggtaagaagt tggaagattt gtctccatcc
3061 actcacaact tggaagttcc atttgtcaag agaagcgaat accaattgtt ggatattgat
3121 gatggctact tgtccttgat gaccatgaac ggtgaaacca aagacgatgt caacgcacca
3181 gaaggtaggt tgggtgacag catgcaagct gctttcgatg aaggtaagga tttgatggtc
3241 actatcattt ctgccatggg tgaagaagct gccatctcct tcaaggaagc tccaagatcc
3301 tctacgccgg acgcatcgtg gccggcatca ccggcgccac aggtgcggtt gctggcgcct
3361 atatcgccga catcaccgat ggggaagatc gggctcgcca cttcgggctc atgagcgctt
3421 gtttcggcgt gggtatggtg gcaggccccg tggccggggg actgttgggc gccatctcct
3481 tgcatgcacc attccttgcg gcggcggtgc tcaacggcct caacctacta ctgggctgct
3541 tcctaatgca ggagtcgcat aagggagagc gtcgaccgat gcccttgaga gccttcaacc
3601 cagtcagctc cttccggtgg gcgcggggca tgactatcgt cgccgcactt atgactgtct
3661 tctttatcat gcaactcgta ggacaggtgc cggcagcgct ctgggtcatt ttcggcgagg
3721 accgctttcg ctggagcgcg acgatgatcg gcctgtcgct tgcggtattc ggaatcttgc
3781 acgccctcgc tcaagccttc gtcactggtc ccgccaccaa acgtttcggc gagaagcagg
3841 ccattatcgc cggcatggcg gccgacgcgc tgggctacgt cttgctggcg ttcgcgacgc
3901 gaggctggat ggccttcccc attatgattc ttctcgcttc cggcggcatc gggatgcccg
3961 cgttgcaggc catgctgtcc aggcaggtag atgacgacca tcagggacag cttcaaggat
4021 cgctcgcggc tcttaccagc ctaacttcga tcactggacc gctgatcgtc acggcgattt
4081 atgccgcctc ggcgagcaca tggaacgggt tggcatggat tgtaggcgcc gccctatacc
4141 ttgtctgcct ccccgcgttg cgtcgcggtg catggagccg ggccacctcg acctgaatgg
4201 aagccggcgg cacctcgcta acggattcac cactccaaga attggagcca atcaattctt
4261 gcggagaact gtgaatgcgc aaaccaaccc ttggcagaac atatccatcg cgtccgccat
4321 ctccagcagc cgcacgcggc gcatctcggg gggggggggg ggggggggga gcttttcaat
4381 tcaattcatc attttttttt tattcttttt tttgatttcg gtttctttga aatttttttg
4441 attcggtaat ctccgaacag aaggaagaac gaaggaagga gcacagactt agattggtat
4501 atatacgcat atgtagtgtt gaagaaacat gaaattgccc agtattctta acccaactgc
4561 acagaacaaa aacctgcagg aaacgaagat aaatcatgtc gaaagctaca tataaggaac
4621 gtgctgctac tcatcctagt cctgttgctg ccaagctatt taatatcatg cacgaaaagc
4681 aaacaaactt gtgtgcttca ttggatgttc gtaccaccaa ggaattactg gagttagttg
4741 aagcattagg tcccaaaatt tgtttactaa aaacacatgt ggatatcttg actgattttt
4801 ccatggaggg cacagttaag ccgctaaagg cattatccgc caagtacaat tttttactct
4861 tcgaagacag aaaatttgct gacattggta atacagtcaa attgcagtac tctgcgggtg
4921 tatacagaat agcagaatgg gcagacatta cgaatgcaca cggtgtggtg ggcccaggta
4981 ttgttagcgg tttgaagcag gcggcagaag aagtaacaaa ggaacctaga ggccttttga
5041 tgttagcaga attgtcatgc aagggctccc tatctactgg agaatatact aagggtactg
5101 ttgacattgc gaagagcgac aaagattttg ttatcggctt tattgctcaa agagacatgg
5161 gtggaagaga tgaaggttac gattggttga ttatgacacc cggtgtgggt ttagatgaca
5221 agggagacgc attgggtcaa cagtatagaa ccgtggatga tgtggtctct acaggatctg
5281 acattattat tgttggaaga ggactatttg caaagggaag ggatgctaag gtagagggtg
5341 aacgttacag aaaagcaggc tgggaagcat atttgagaag atgcggccag caaaactaaa
5401 aaactgtatt ataagtaaat gcatgtatac taaactcaca aattagagct tcaatttaat
5461 tatatcagtt attacccggg aatctcggtc gtaatgattt ttataatgac gaaaaaaaaa
5521 aaattggaaa gaaaaagctc cccccccccc ccccccccct cgggcagcgt tgggtcctgg
5581 ccacgggtgc gcatgatcgt gctcctgtcg ttgaggaccc ggctaggctg gcggggttgc
5641 cttactggtt agcagaatga atcaccgata cgcgagcgaa cgtgaagcga ctgctgctgc
5701 aaaacgtctg cgacctgagc aacaacatga atggtcttcg gtttccgtgt ttcgtaaagt
5761 ctggaaacgc ggaagtcagc gccctgcacc attatgttcc ggatctgcat cgcaggatgc
5821 tgctggctac cctgtggaac acctacatct gtattaacga agcgctggca ttgaccctga
5881 gtgatttttc tctggtcccg ccgcatccat accgccagtt gtttaccctc acaacgttcc
5941 agtaaccggg catgttcatc atcagtaacc cgtatcgtga gcatcctctc tcgtttcatc
6001 ggtatcatta cccccatgaa cagaaatccc ccttacacgg aggcatcagt gaccaaacag
6061 gaaaaaaccg cccttaacat ggcccgcttt atcagaagcc agacattaac gcttctggag
6121 aaactcaacg agctggacgc ggatgaacag gcagacatct gtgaatcgct tcacgaccac
6181 gctgatgagc tttaccgcag gactaatgtt gtgggaaatt ggagcgataa gcgtgcttct
6241 gccgtggcca ggacaacgta tactcatcag ataacagcaa tacctgatca ctacttcgca
6301 ctagtttctc ggtactatgc atatgatcca atatcaaagg aaatgatagc attgaaggat
6361 gagactaatc caattgagga gtggcagcat atagaacagc taaagggtag tgctgaagga
6421 agcatacgat accccgcatg gaatgggata atatcacagg aggtactaga ctacctttca
6481 tcctacataa atagacgcat ataagtacgc atttaagcat aaacacgcac tatgccgttc
6541 ttctcatgta tatatatata caggcaacac gcagatatag gtgcgacgtg aacagtgagc
6601 tgtatgtgcg cagctcgcgt tgcattttcg gaagcgctcg ttttcggaaa cgctttgaag
6661 ttcctattcc gaagttccta ttctctagaa agtataggaa cttcagagcg cttttgaaaa
6721 ccaaaagcgc tctgaagacg cactttcaaa aaaccaaaaa cgcaccggac tgtaacgagc
6781 tactaaaata ttgcgaatac cgcttccaca aacattgctc aaaagtatct ctttgctata
6841 tatctctgtg ctatatccct atataaccta cccatccacc tttcgctcct tgaacttgca
6901 tctaaactcg acctctacat tttttatgtt tatctctagt attactcttt agacaaaaaa
6961 attgtagtaa gaactattca tagagtgaat cgaaaacaat acgaaaatgt aaacatttcc
7021 tatacgtagt atatagagac aaaatagaag aaaccgttca taattttctg accaatgaag
7081 aatcatcaac gctatcactt tctgttcaca aagtatgcgc aatccacatc ggtatagaat
7141 ataatcgggg atgcctttat cttgaaaaaa tgcacccgca gcttcgctag taatcagtaa
7201 acgcgggaag tggagtcagg ctttttttat ggaagagaaa atagacacca aagtagcctt
7261 cttctaacct taacggacct acagtgcaaa aagttatcaa gagactgcat tatagagcgc
7321 acaaaggaga aaaaaagtaa tctaagatgc tttgttagaa aaatagcgct ctcgggatgc
7381 atttttgtag aacaaaaaag aagtatagat tctttgttgg taaaatagcg ctctcgcgtt
7441 gcatttctgt tctgtaaaaa tgcagctcag attctttgtt tgaaaaatta gcgctctcgc
7501 gttgcatttt tgttttacaa aaatgaagca cagattcttc gttggtaaaa tagcgctttc
7561 gcgttgcatt tctgttctgt aaaaatgcag ctcagattct ttgtttgaaa aattagcgct
7621 ctcgcgttgc atttttgttc tacaaaatga agcacagatg cttcgttctg cctcgcgcgt
7681 ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt
7741 ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg
7801 tgtcggggcg cagccatgac ccagtcacgt agcgatagcg gagtgtatac tggcttaact
7861 atgcggcatc agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca
7921 gatgcgtaag gagaaaatac cgcatcaggc gctcttccgc ttcctcgctc actgactcgc
7981 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
8041 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
8101 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
8161 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
8221 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
8281 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
8341 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
8401 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
8461 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
8521 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag
8581 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
8641 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
8701 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
8761 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
8821 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
8881 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
8941 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
9001 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
9061 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
9121 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
9181 atagtttgcg caacgttgtt gccattgctg caggcatcgt ggtgtcacgc tcgtcgtttg
9241 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
9301 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
9361 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
9421 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
9481 ggcgaccgag ttgctcttgc ccggcgtcaa cacgggataa taccgcgcca catagcagaa
9541 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
9601 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
9661 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
9721 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
9781 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
9841 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca
9901 ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtcttcaag
9961 aa
//