LOCUS LT727553 9214 bp DNA circular SYN 06-FEB-2017
DEFINITION Yeast expression vector pEX2, complete sequence.
ACCESSION LT727553
VERSION LT727553.1
KEYWORDS .
SOURCE Yeast expression vector pEX2
ORGANISM Yeast expression vector pEX2
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 9214)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..9214
/organism="Yeast expression vector pEX2"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 2890."
/db_xref="taxon:1945470"
rep_origin 1..838
/note="ARS1 ori"
regulatory complement(839..1109)
/note="CYC1 terminator"
/regulatory_class="terminator"
regulatory complement(1229..2210)
/note="CYC1 promoter"
/regulatory_class="promoter"
CDS 3848..4651
/transl_table=11
/note="URA3"
/protein_id="SJL88821.1"
/translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKEL
LELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVK
LQYSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLS
TGEYTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYR
TVDDVVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
regulatory 5757..5861
/note="FLP terminator"
/regulatory_class="terminator"
rep_origin 5939..6919
/note="2mu ori"
rep_origin complement(7203..7605)
/note="pMB1 ori"
CDS complement(8146..9006)
/transl_table=11
/note="ampicillin"
/protein_id="SJL88822.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
BASE COUNT 2496 a 2102 c 2098 g 2518 t
ORIGIN
1 ttccacatgt taaaatagtg aaggagcatg ttcggcacac agtggaccga acgtggggta
61 agtgcactag ggtccggtta aacggatctc gcattgatga ggcaacgcta attatcaaca
121 tatagattgt tatctatctg catgaacacg aaatctttac ttgacgactt gaggctgatg
181 gtgtttatgc aaagaaacca ctgtgtttaa tatgtgtcac tgtttgatat tactgtcagc
241 gtagaagata atagtaaaag cggttaataa gtgtatttga gataagtgtg ataaagtttt
301 tacagcgaaa agacgataaa tacaagaaaa tgattacgag gatacggaga gaggtatgta
361 catgtgtatt tatatactaa gctgccggcg gttgtttgca agaccgagaa aaggctagca
421 agaatcgggt cattgtagcg tatgcgcctg tgaacattct cttcaacaag tttgattcca
481 ttgcggtgaa atggtaaaag tcaaccccct gcgatgtata ttttcctgta caatcaatca
541 aaaagccaaa tgatttagca ttatctttac atcttgttat tttacagatt ttatgtttag
601 atcttttatg cttgcttttc aaaaggcctg caggcaagtg cacaaacaat acttaaataa
661 atactactca gtaataacct atttcttagc atttttgacg aaatttgcta ttttgttaga
721 gtcttttaca ccatttgtct ccacacctcc gcttacatca acaccaataa cgccatttaa
781 tctaagcgca tcaccaacat tttctggcgt cagtccacca gctaacataa aatgtaagct
841 tgcaaattaa agccttcgag cgtcccaaaa ccttctcaag caaggttttc agtataatgt
901 tacatgcgta cacgcgtctg tacagaaaaa aaagaaaaat ttgaaatata aataacgttc
961 ttaatactaa cataactata aaaaaataaa tagggaccta gacttcaggt tgtctaactc
1021 cttccttttc ggttagagcg gatgtggggg gagggcgtga atgtaagcgt gacataacta
1081 attacatgat atcgacaaag gaaaaggggg acggatcccc gggaattcag ttattattaa
1141 tttagtgtgt gtatttgtgt ttgcgtgtct atagaagtat agtaatttat gctacaaagg
1201 acctaatgta taaggaaaga atatttagag aaaagaagaa aacaagagtt ttatatacat
1261 acagagcaca tgcatgccat atgatcatgt gtcgtcgcac acatatatat atgcctgtat
1321 gtgtcagcac taaagttgcc tggccatcca cgctatatac acgcctggcg gatctgctcg
1381 aggattgcct acgcgtgggc ttgatccacc aaccaacgct cgccaaatga gctggcgctt
1441 ggtcttcggt catcgtccgt aaaccccggc caaagagacc ggaaagatcg gtgaaaacat
1501 cttgatcttg ctcccgggaa ttttagattc aggtaggaaa ttgattacat aaatactgtt
1561 accctgaatc atattcgacg atgtcgtctc acacggaaat ataattcatt tcttggtttt
1621 ccaaaaaaat tttcattttt tttcactttt ttgtttcgtc ctcctttttt ttttttttta
1681 ttttttttcc tgtgttcacc tttttttttt ttttttttca gtttacaact ttctgcattc
1741 ttttctgtgt tttttttttt ttcgtttttc catttgttcg ttcgttgcct gtttttttgc
1801 cctattgttc tcgagcctaa aaattttttc ctttcctgct ttcctttctt cgttcaaagt
1861 ttcctattcc attgttctct tcggtaaact cattgttgct cggaactcag atatattcag
1921 gtcaatttac tgtacttcaa ttgacttttt tcttgaaatt tcaacttgcc tttcaacttg
1981 ttcttctttt ttaattctta ttctacactt tagttccctt accttgttcc taattattgt
2041 ctgcaaaaga aaacatacac ctatttcatt cacacactaa aacatgtctg acgaagaaca
2101 caacctttga aaatgctgac gctggtgcct ccgccaccta cccaatgcaa tgttctgcct
2161 tgagaaagaa tggtttcgtt gtcatcaaag gtagaccatg taagattgtc gacatgtcca
2221 cttccaagac cggtaagcac ggtcacgcca aggtccattt ggttaccctt gatatcttca
2281 ctggtaagaa gttggaagat ttgtctccat ccactcacaa cttggaagtt ccatttgtca
2341 agagaagcga ataccaattg ttggatattg atgatggcta cttgtccttg atgaccatga
2401 acggtgaaac caaagacgat gtcaacgcac cagaaggtag gttgggtgac agcatgcaag
2461 ctgctttcga tgaaggtaag gatttgatgg tcactatcat ttctgccatg ggtgaagaag
2521 ctgccatctc cttcaaggaa gctccaagat cctctacgcc ggacgcatcg tggccggcat
2581 caccggcgcc acaggtgcgg ttgctggcgc ctatatcgcc gacatcaccg atggggaaga
2641 tcgggctcgc cacttcgggc tcatgagcgc ttgtttcggc gtgggtatgg tggcaggccc
2701 cgtggccggg ggactgttgg gcgccatctc cttgcatgca ccattccttg cggcggcggt
2761 gctcaacggc ctcaacctac tactgggctg cttcctaatg caggagtcgc ataagggaga
2821 gcgtcgaccg atgcccttga gagccttcaa cccagtcagc tccttccggt gggcgcgggg
2881 catgactatc gtcgccgcac ttatgactgt cttctttatc atgcaactcg taggacaggt
2941 gccggcagcg ctctgggtca ttttcggcga ggaccgcttt cgctggagcg cgacgatgat
3001 cggcctgtcg cttgcggtat tcggaatctt gcacgccctc gctcaagcct tcgtcactgg
3061 tcccgccacc aaacgtttcg gcgagaagca ggccattatc gccggcatgg cggccgacgc
3121 gctgggctac gtcttgctgg cgttcgcgac gcgaggctgg atggccttcc ccattatgat
3181 tcttctcgct tccggcggca tcgggatgcc cgcgttgcag gccatgctgt ccaggcaggt
3241 agatgacgac catcagggac agcttcaagg atcgctcgcg gctcttacca gcctaacttc
3301 gatcactgga ccgctgatcg tcacggcgat ttatgccgcc tcggcgagca catggaacgg
3361 gttggcatgg attgtaggcg ccgccctata ccttgtctgc ctccccgcgt tgcgtcgcgg
3421 tgcatggagc cgggccacct cgacctgaat ggaagccggc ggcacctcgc taacggattc
3481 accactccaa gaattggagc caatcaattc ttgcggagaa ctgtgaatgc gcaaaccaac
3541 ccttggcaga acatatccat cgcgtccgcc atctccagca gccgcacgcg gcgcatctcg
3601 gggggggggg gggggggggg gagcttttca attcaattca tcattttttt tttattcttt
3661 tttttgattt cggtttcttt gaaatttttt tgattcggta atctccgaac agaaggaaga
3721 acgaaggaag gagcacagac ttagattggt atatatacgc atatgtagtg ttgaagaaac
3781 atgaaattgc ccagtattct taacccaact gcacagaaca aaaacctgca ggaaacgaag
3841 ataaatcatg tcgaaagcta catataagga acgtgctgct actcatccta gtcctgttgc
3901 tgccaagcta tttaatatca tgcacgaaaa gcaaacaaac ttgtgtgctt cattggatgt
3961 tcgtaccacc aaggaattac tggagttagt tgaagcatta ggtcccaaaa tttgtttact
4021 aaaaacacat gtggatatct tgactgattt ttccatggag ggcacagtta agccgctaaa
4081 ggcattatcc gccaagtaca attttttact cttcgaagac agaaaatttg ctgacattgg
4141 taatacagtc aaattgcagt actctgcggg tgtatacaga atagcagaat gggcagacat
4201 tacgaatgca cacggtgtgg tgggcccagg tattgttagc ggtttgaagc aggcggcaga
4261 agaagtaaca aaggaaccta gaggcctttt gatgttagca gaattgtcat gcaagggctc
4321 cctatctact ggagaatata ctaagggtac tgttgacatt gcgaagagcg acaaagattt
4381 tgttatcggc tttattgctc aaagagacat gggtggaaga gatgaaggtt acgattggtt
4441 gattatgaca cccggtgtgg gtttagatga caagggagac gcattgggtc aacagtatag
4501 aaccgtggat gatgtggtct ctacaggatc tgacattatt attgttggaa gaggactatt
4561 tgcaaaggga agggatgcta aggtagaggg tgaacgttac agaaaagcag gctgggaagc
4621 atatttgaga agatgcggcc agcaaaacta aaaaactgta ttataagtaa atgcatgtat
4681 actaaactca caaattagag cttcaattta attatatcag ttattacccg ggaatctcgg
4741 tcgtaatgat ttttataatg acgaaaaaaa aaaaattgga aagaaaaagc tccccccccc
4801 cccccccccc ctcgggcagc gttgggtcct ggccacgggt gcgcatgatc gtgctcctgt
4861 cgttgaggac ccggctaggc tggcggggtt gccttactgg ttagcagaat gaatcaccga
4921 tacgcgagcg aacgtgaagc gactgctgct gcaaaacgtc tgcgacctga gcaacaacat
4981 gaatggtctt cggtttccgt gtttcgtaaa gtctggaaac gcggaagtca gcgccctgca
5041 ccattatgtt ccggatctgc atcgcaggat gctgctggct accctgtgga acacctacat
5101 ctgtattaac gaagcgctgg cattgaccct gagtgatttt tctctggtcc cgccgcatcc
5161 ataccgccag ttgtttaccc tcacaacgtt ccagtaaccg ggcatgttca tcatcagtaa
5221 cccgtatcgt gagcatcctc tctcgtttca tcggtatcat tacccccatg aacagaaatc
5281 ccccttacac ggaggcatca gtgaccaaac aggaaaaaac cgcccttaac atggcccgct
5341 ttatcagaag ccagacatta acgcttctgg agaaactcaa cgagctggac gcggatgaac
5401 aggcagacat ctgtgaatcg cttcacgacc acgctgatga gctttaccgc aggactaatg
5461 ttgtgggaaa ttggagcgat aagcgtgctt ctgccgtggc caggacaacg tatactcatc
5521 agataacagc aatacctgat cactacttcg cactagtttc tcggtactat gcatatgatc
5581 caatatcaaa ggaaatgata gcattgaagg atgagactaa tccaattgag gagtggcagc
5641 atatagaaca gctaaagggt agtgctgaag gaagcatacg ataccccgca tggaatggga
5701 taatatcaca ggaggtacta gactaccttt catcctacat aaatagacgc atataagtac
5761 gcatttaagc ataaacacgc actatgccgt tcttctcatg tatatatata tacaggcaac
5821 acgcagatat aggtgcgacg tgaacagtga gctgtatgtg cgcagctcgc gttgcatttt
5881 cggaagcgct cgttttcgga aacgctttga agttcctatt ccgaagttcc tattctctag
5941 aaagtatagg aacttcagag cgcttttgaa aaccaaaagc gctctgaaga cgcactttca
6001 aaaaaccaaa aacgcaccgg actgtaacga gctactaaaa tattgcgaat accgcttcca
6061 caaacattgc tcaaaagtat ctctttgcta tatatctctg tgctatatcc ctatataacc
6121 tacccatcca cctttcgctc cttgaacttg catctaaact cgacctctac attttttatg
6181 tttatctcta gtattactct ttagacaaaa aaattgtagt aagaactatt catagagtga
6241 atcgaaaaca atacgaaaat gtaaacattt cctatacgta gtatatagag acaaaataga
6301 agaaaccgtt cataattttc tgaccaatga agaatcatca acgctatcac tttctgttca
6361 caaagtatgc gcaatccaca tcggtataga atataatcgg ggatgccttt atcttgaaaa
6421 aatgcacccg cagcttcgct agtaatcagt aaacgcggga agtggagtca ggcttttttt
6481 atggaagaga aaatagacac caaagtagcc ttcttctaac cttaacggac ctacagtgca
6541 aaaagttatc aagagactgc attatagagc gcacaaagga gaaaaaaagt aatctaagat
6601 gctttgttag aaaaatagcg ctctcgggat gcatttttgt agaacaaaaa agaagtatag
6661 attctttgtt ggtaaaatag cgctctcgcg ttgcatttct gttctgtaaa aatgcagctc
6721 agattctttg tttgaaaaat tagcgctctc gcgttgcatt tttgttttac aaaaatgaag
6781 cacagattct tcgttggtaa aatagcgctt tcgcgttgca tttctgttct gtaaaaatgc
6841 agctcagatt ctttgtttga aaaattagcg ctctcgcgtt gcatttttgt tctacaaaat
6901 gaagcacaga tgcttcgttc tgcctcgcgc gtttcggtga tgacggtgaa aacctctgac
6961 acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag
7021 cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg cgcagccatg acccagtcac
7081 gtagcgatag cggagtgtat actggcttaa ctatgcggca tcagagcaga ttgtactgag
7141 agtgcaccat atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag
7201 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
7261 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
7321 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
7381 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
7441 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
7501 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
7561 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
7621 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
7681 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
7741 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
7801 gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc
7861 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
7921 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
7981 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
8041 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
8101 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
8161 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
8221 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
8281 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
8341 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
8401 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
8461 tgcaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
8521 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
8581 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
8641 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
8701 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
8761 aacacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
8821 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
8881 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
8941 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
9001 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
9061 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
9121 ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa
9181 taggcgtatc acgaggccct ttcgtcttca agaa
//