LOCUS LT727551 4480 bp DNA circular SYN 06-FEB-2017
DEFINITION Vector pBluhHemeOx, complete sequence.
ACCESSION LT727551
VERSION LT727551.1
KEYWORDS .
SOURCE Vector pBluhHemeOx
ORGANISM Vector pBluhHemeOx
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 4480)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..4480
/organism="Vector pBluhHemeOx"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 2866."
/db_xref="taxon:1945257"
rep_origin 4..459
/note="f1 ori"
regulatory 626..642
/note="T7"
/regulatory_class="promoter"
misc_feature complement(710..1312)
/note="3UTR"
CDS complement(1313..2179)
/transl_table=11
/note="hHOx"
/protein_id="SJL88817.1"
/translation="MERPQPDSMPQDLSEALKEATKEVHTQAENAEFMRNFQKGQVTR
DGFKLVMASLYHIYVALEEEIERNKESPVFAPVYFPEELHRKAALEQDLAFWYGPRWQ
EVIPYTPAMQRYVKRLHEVGRTEPELLVAHAYTRYLGDLSGGQVLKKIAQKALDLPSS
GEGLAFFTFPNIASATKFKQLYRSRMNSLEMTPAVRQRVIEEAKTAFLLNIQLFEELQ
ELLTHDTKDQSPSRAPGLRQRASNKVQDSAPVETPRGKPPLNTRSQAPLLRWVLTLSF
LVATVAVGLYAM"
misc_feature complement(2180..2216)
/note="5UTR"
regulatory complement(2294..2310)
/note="T3"
/regulatory_class="promoter"
regulatory complement(2374..2457)
/note="lac"
/regulatory_class="promoter"
rep_origin complement(2549..2951)
/note="pMB1 ori"
CDS complement(3492..4352)
/transl_table=11
/note="ampicillin resistance"
/protein_id="SJL88818.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
BASE COUNT 1033 a 1181 c 1177 g 1083 t
ORIGIN
1 ctgacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
61 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
121 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
181 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
241 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
301 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
361 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
421 ttaacgcgaa ttttaacaaa atattaacgc ttacaatttc cattcgccat tcaggctgcg
481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tgggtaccgg
661 gccccccctc gaggtcgacg gtatcgataa gcttgatatc gaattcnnnc agacaatgtt
721 gtttattatt tcacacaaaa gttagaccaa ggccacagtg ccgttaaaca cctccctccc
781 cacccacgca tggctcaaaa accaccccaa ccctgctata aaaacaacag aacacaacaa
841 aaatactgca tttgaggctg agccaggaac agagtggctc caacaaaaat aagacaaaaa
901 cacaagacac aacatctttt caggtttgtg taaaaattgc catggcccac ggggaaagtg
961 gtcatggccg tgtcaacaag gatacttgaa actcagggct tttggaggtt tgagacagct
1021 gccacattag ggtgtcttcc aggctctgct gcaggaactg aggatgctga agggcagggc
1081 tgaccccttc gccccctctg aagtttaggc cattgcctgg atgtgctttt cgttggggaa
1141 gatgccatag gctccttcct cctttccaga gagagggaca cagtgagagg gctggaggcc
1201 ctgaaagcct tcagtgccca cggtaaggaa gccagccaag agaattccct ctctagaaag
1261 aaggccttcc accggacaaa gttcatggcc ctgggagcca gcatgcctgc attcacatgg
1321 cataaagccc tacagcaact gtcgccacca gaaagctgag tgtaaggacc catcggagaa
1381 gcggagcctg ggagcgggtg ttgagtgggg gcttccctct gggagtctcc acgggggcag
1441 aatcttgcac tttgttgctg gcccgctggc gaagccctgg tgcccgtgag gggctctggt
1501 ccttggtgtc atgggtcagc agctcctgca actcctcaaa gagctggatg ttgagcagga
1561 acgcagtctt ggcctcttct atcaccctct gcctgactgc gggagtcatc tccagggagt
1621 tcatgcggga gcggtagagc tgcttgaact tggtggcact ggcaatgttg gggaaggtga
1681 agaaggccag gccctcgcca gagctgggca ggtccagggc tttctgggca atctttttga
1741 gcacctggcc cccagacagg tcacccaggt agcgggtgta ggcgtgggcc accagcagct
1801 cgggctctgt gcgccccacc tcgtggagcc gcttcacata gcgctgcatg gctggtgtgt
1861 aggggatgac ctcctgccag cggggcccgt accagaaggc caggtcctgc tccagggcag
1921 ccttgcggtg cagctcttct gggaagtaga caggggcgaa gactgggctc tccttgttgc
1981 gctcaatctc ctcctccagg gccacataga tgtggtacag ggaggccatc accagcttga
2041 agccgtctcg ggtcacctgg cccttctgaa agttcctcat gaactcagca ttctctgcct
2101 gggtgtgcac ctccttggtg gcctccttca gggcctctga caaatcctgg ggcatgctgt
2161 cgggttgcgg acgctccatc cggccggtgc tgggctcgtt cgtgctggct cagagtnnng
2221 aattcctgca gcccggggga tccactagtt ctagagcggc cgccaccgcg gtggagctcc
2281 agcttttgtt ccctttagtg agggttaatt gcgcgcttgg cgtaatcatg gtcatagctg
2341 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
2401 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca
2461 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc
2521 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg
2581 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
2641 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
2701 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
2761 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
2821 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
2881 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
2941 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
3001 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
3061 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
3121 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta
3181 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
3241 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
3301 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
3361 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
3421 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
3481 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
3541 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
3601 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
3661 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
3721 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
3781 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
3841 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
3901 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
3961 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
4021 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
4081 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
4141 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
4201 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
4261 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
4321 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
4381 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
4441 caaatagggg ttccgcgcac atttccccga aaagtgccac
//