LOCUS LT727550 5962 bp DNA circular SYN 06-FEB-2017
DEFINITION Vector pLT10T3A20, complete sequence.
ACCESSION LT727550
VERSION LT727550.1
KEYWORDS .
SOURCE Vector pLT10T3A20
ORGANISM Vector pLT10T3A20
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 5962)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..5962
/organism="Vector pLT10T3A20"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 2862."
/db_xref="taxon:1945330"
regulatory 24..40
/note="T7"
/regulatory_class="promoter"
misc_feature 41..103
/note="5UTR"
CDS 104..2476
/transl_table=11
/note="hA20"
/protein_id="SJL88815.1"
/translation="MAEQVLPQALYLSNMRKAVKIRERTPEDIFKPTNGIIHHFKTMH
RYTLEMFRTCQFCPQFREIIHKALIDRNIQATLESQKKLNWCREVRKLVALKTNGDGN
CLMHATSQYMWGVQDTDLVLRKALFSTLKETDTRNFKFRWQLESLKSQEFVETGLCYD
TRNWNDEWDNLIKMASTDTPMARSGLQYNSLEEIHIFVLCNILRRPIIVISDKMLRSL
ESGSNFAPLKVGGIYLPLHWPAQECYRYPIVLGYDSHHFVPLVTLKDSGPEIRAVPLV
NRDRGRFEDLKVHFLTDPENEMKEKLLKEYLMVIEIPVQGWDHGTTHLINAAKLDEAN
LPKEINLVDDYFELVQHEYKKWQENSEQGRREGHAQNPMEPSVPQLSLMDVKCETPNC
PFFMSVNTQPLCHECSERRQKNQNKLPKLNSKPGPEGLPGMALGASRGEAYEPLAWNP
EESTGGPHSAPPTAPSPFLFSETTAMKCRSPGCPFTLNVQHNGFCERCHNARQLHASH
APDHTRHLDPGKCQACLQDVTRTFNGICSTCFKRTTAEASSSLSTSLPPSCHQRSKSD
PSRLVRSPSPHSCHRAGNDAPAGCLSQAARTPGDRTGTSKCRKAGCVYFGTPENKGFC
TLCFIEYRENKHFAAASGKVSPTASRFQNTIPCLGRECGTLGSTMFEGYCQKCFIEAQ
NQRFHEAKRTEEQLRSSQRRDVPRTTQSTSRPKCARASCKNILACRSEELCMECQHPN
QRMGPGAHRGEPAPEDPPKQRCRAPACDHFGNAKCNGYCNECFQFKQMYG"
regulatory complement(2557..2574)
/note="T3"
/regulatory_class="promoter"
regulatory 2591..2638
/note="T7T"
/regulatory_class="terminator"
regulatory 2712..2759
/note="T7T"
/regulatory_class="terminator"
regulatory 2828..2909
/note="fdT"
/regulatory_class="terminator"
regulatory 2938..3019
/note="fdT"
/regulatory_class="terminator"
rep_origin 3117..3572
/note="f1 ori"
rep_origin complement(3576..3949)
/note="pMB1 ori"
CDS complement(4490..5350)
/transl_table=11
/note="ampicillin resistance"
/protein_id="SJL88816.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
regulatory 5716..5847
/note="PL"
/regulatory_class="promoter"
BASE COUNT 1568 a 1529 c 1438 g 1427 t
ORIGIN
1 ttccggatct cgatcccgga aattaatacg actcactata gggagaccac aacggtttcc
61 ctctagaaat aattttgttt aactttaaga aggagatata catatggctg aacaagtcct
121 tcctcaggct ttgtatttga gcaatatgcg gaaagctgtg aagatacggg agagaactcc
181 agaagacatt tttaaaccta ctaatgggat cattcatcat tttaaaacca tgcaccgata
241 cacactggaa atgttcagaa cttgccagtt ttgtcctcag tttcgggaga tcatccacaa
301 agccctcatc gacagaaaca tccaggccac cctggaaagc cagaagaaac tcaactggtg
361 tcgagaagtc cggaagcttg tggcgctgaa aacgaacggt gacggcaatt gcctcatgca
421 tgccacttct cagtacatgt ggggcgttca ggacacagac ttggtactga ggaaggcgct
481 gttcagcacg ctcaaggaaa cagacacacg caactttaaa ttccgctggc aactggagtc
541 tctcaaatct caggaatttg ttgaaacggg gctttgctat gatactcgga actggaatga
601 tgaatgggac aatcttatca aaatggcttc cacagacaca cccatggccc gaagtggact
661 tcagtacaac tcactggaag aaatacacat atttgtcctt tgcaacatcc tcagaaggcc
721 aatcattgtc atttcagaca aaatgctaag aagtttggaa tcaggttcca atttcgcccc
781 tttgaaagtg ggtggaattt acttgcctct ccactggcct gcccaggaat gctacagata
841 ccccattgtt ctcggctatg acagccatca ttttgtaccc ttggtgaccc tgaaggacag
901 tgggcctgaa atccgagctg ttccacttgt taacagagac cggggaagat ttgaagactt
961 aaaagttcac tttttgacag atcctgaaaa tgagatgaag gagaagctct taaaagagta
1021 cttaatggtg atagaaatcc ccgtccaagg ctgggaccat ggcacaactc atctcatcaa
1081 tgccgcaaag ttggatgaag ctaacttacc aaaagaaatc aatctggtag atgattactt
1141 tgaacttgtt cagcatgagt acaagaaatg gcaggaaaac agcgagcagg ggaggagaga
1201 ggggcacgcc cagaatccca tggaaccttc cgtgccccag ctttctctca tggatgtaaa
1261 atgtgaaacg cccaactgcc ccttcttcat gtctgtgaac acccagcctt tatgccatga
1321 gtgctcagag aggcggcaaa agaatcaaaa caaactccca aagctgaact ccaagccggg
1381 ccctgagggg ctccctggca tggcgctcgg ggcctctcgg ggagaagcct atgagccctt
1441 ggcgtggaac cctgaggagt ccactggggg gcctcattcg gccccaccga cagcacccag
1501 cccttttctg ttcagtgaga ccactgccat gaagtgcagg agccccggct gccccttcac
1561 actgaatgtg cagcacaacg gattttgtga acgttgccac aacgcccggc aacttcacgc
1621 cagccacgcc ccagaccaca caaggcactt ggatcccggg aagtgccaag cctgcctcca
1681 ggatgttacc aggacattta atgggatctg cagtacttgc ttcaaaagga ctacagcaga
1741 ggcctcctcc agcctcagca ccagcctccc tccttcctgt caccagcgtt ccaagtcaga
1801 tccctcgcgg ctcgtccgga gcccctcccc gcattcttgc cacagagctg gaaacgacgc
1861 ccctgctggc tgcctgtctc aagctgcacg gactcctggg gacaggacgg ggacgagcaa
1921 gtgcagaaaa gccggctgcg tgtattttgg gactccagaa aacaagggct tttgcacact
1981 gtgtttcatc gagtacagag aaaacaaaca ttttgctgct gcctcaggga aagtcagtcc
2041 cacagcgtcc aggttccaga acaccattcc gtgcctgggg agggaatgcg gcacccttgg
2101 aagcaccatg tttgaaggat actgccagaa gtgtttcatt gaagctcaga atcagagatt
2161 tcatgaggcc aaaaggacag aagagcaact gagatcgagc cagcgcagag atgtgcctcg
2221 aaccacacaa agcacctcaa ggcccaagtg cgcccgggcc tcctgcaaga acatcctggc
2281 ctgccgcagc gaggagctct gcatggagtg tcagcatccc aaccagagga tgggccctgg
2341 ggcccaccgg ggtgagcctg cccccgaaga cccccccaag cagcgttgcc gggcccccgc
2401 ctgtgatcat tttggcaatg ccaagtgcaa cggctactgc aacgaatgct ttcagttcaa
2461 gcagatgtat ggctaaccgg aaacaggtgg gtcacctcct gcaagaagtg gggcctcgag
2521 gtcgacgaat tcgagctcgg ccgacttggc cttcccttta gtgagggtta ataaacttgg
2581 tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct
2641 gaaaggagga actatatgcg ctcatacgat atgaacgttg agactgccgc tgagttatca
2701 gtgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc
2761 tgaaaggagg aactatatcc ggatagctta tcgctagagg tcgaaattca cctcgaaagc
2821 aagctgataa accgatacaa ttaaaggctc cttttggagc cttttttttt ggagattttc
2881 aacgtgaaaa aattattatt cgcaattcca agctaattca cctcgaaagc aagctgataa
2941 accgatacaa ttaaaggctc cttttggagc cttttttttt ggagattttc aacgtgaaaa
3001 aattattatt cgcaattcca agctctgcct cgcgcgtttc ggtgatgacg gtgaaaacct
3061 ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg cagatcacgc
3121 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac
3181 acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt
3241 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc
3301 tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc
3361 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact
3421 cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg
3481 gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc
3541 gaattttaac aaaatattaa cgtttacaat ttgatctgcg ctcggtcgtt cggctgcggc
3601 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
3661 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
3721 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
3781 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
3841 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
3901 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
3961 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
4021 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
4081 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
4141 agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga
4201 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
4261 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
4321 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
4381 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
4441 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
4501 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
4561 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
4621 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
4681 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
4741 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
4801 ttgctgcagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
4861 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
4921 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
4981 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
5041 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
5101 cgtcaacacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
5161 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
5221 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
5281 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
5341 gaatactcat actcttcctt tttcaatatt atgtaagcag acagttttat tgttcatgat
5401 gatatatttt tatcttgtgc aatgtaacat cagagatttt gagacacaac gtggctttgt
5461 tgaataaatc gaacttttgc tgagttgaag gatcagatca cgcatcttcc cgacaacgca
5521 gaccgttccg tggcaaagca aaagttcaaa atcaccaact ggtccaccta caacaaagct
5581 ctcatcaacc gtggctccct cactttctgg ctggatgatg gggcgattca ggcctggtat
5641 gagtcagcaa caccttcttc acgaggcaga cctcagcgcc ggtgatgccg gccacgatgc
5701 gtccggcgta gaggatctct cacctaccaa acaatgcccc cctgcaaaaa ataaattcat
5761 ataaaaaaca tacagataac catctgcggt gataaattat ctctggcggt gttgacataa
5821 ataccactgg cggtgatact gagcacatca gcaggacgca ctgaccacca tgaaggtgac
5881 gctcttaaaa ttaagccctg aagaagggca gcattcaaag cagaaggctt tggggtgtgt
5941 gatacgaaac gaagcattgg aa
//