LOCUS LT727548 8300 bp DNA circular SYN 06-FEB-2017 DEFINITION Vector pPNEcad, complete sequence. ACCESSION LT727548 VERSION LT727548.1 KEYWORDS . SOURCE Vector pPNEcad ORGANISM Vector pPNEcad other sequences; artificial sequences; vectors. REFERENCE 1 (bases 1 to 8300) AUTHORS De Schamphelaire W. JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent, Technologiepark 927, 9052, BELGIUM. REFERENCE 2 AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K., Roggeman Fonseca M., Vanhoucke M., Beyaert R. TITLE BCCM/LMBP Plasmid collection JOURNAL Unpublished. FEATURES Location/Qualifiers source 1..8300 /organism="Vector pPNEcad" /mol_type="other DNA" /note="BCCM/LMBP Plasmid collection (Ghent University,Belgium) accession number LMBP 2828." /db_xref="taxon:1945367" regulatory complement(402..853) /note="3' UTR of mPGK1, incl. polyA" /regulatory_class="terminator" misc_feature complement(912..945) /note="3' UTR of mE-cad" CDS complement(946..3600) /transl_table=11 /note="mE-cad" /protein_id="SJL88809.1" /translation="MGARCRSFSALLLLLQVSSWLCQELEPESCSPGFSSEVYTFPVP ERHLERGHVLGRVRFEGCTGRPRTAFFSEDSRFKVATDGTITVKRHLKLHKLETSFLV RARDSSHRELSTKVTLKSMGHHHHRHHHRDPASESNPELLMFPSVYPGLRRQKRDWVI PPISCPENEKGEFPKNLVQIKSNRDKETKVFYSITGQGADKPPVGVFIIERETGWLKV TQPLDREAIAKYILYSHAVSSNGEAVEDPMEIVITVTDQNDNRPEFTQEVFEGSVAEG AVPGTSVMKVSATDADDDVNTYNAAIAYTIVSQDPELPHKNMFTVNRDTGVISVLTSG LDRESYPTYTLVVQAADLQGEGLSTTAKAVITVKDINDNAPVFNPSTYQGQVPENEVN ARIATLKVTDDDAPNTPAWKAVYTVVNDPDQQFVVVTDPTTNDGILKTAKGLDFEAKQ QYILHVRVENEEPFEGSLVPSTATVTVDVVDVNEAPIFMPAERRVEVPEDFGVGQEIT SYTAREPDTFMDQKITYRIWRDTANWLEINPETGAIFTRAEMDREDAEHVKNSTYVAL IIATDDGSPIATGTGTLLLVLLDVNDNAPIPEPRNMQFCQRNPQPHIITILDPDLPPN TSPFTAELTHGASVNWTIEYNDAAQESLILQPRKDLEIGEYKIHLKLADNQNKDQVTT LDVHVCDCEGTVNNCMKAGIVAAGLQVPAILGILGGILALLILILLLLLFLRRRTVVK EPLLPPDDDTRDNVYYYDEEGGGEEDQDFDLSQLHRGLDARPEVTRNDVAPTLMSVPQ YRPRPANPDEIGNFIDENLKAADSDPTAPPYDSLLVFDYEGSGSEAASLSSLNSSESD QDQDYDYLNEWGNRFKKLADMYGGGEDD" misc_feature complement(3601..3633) /note="5' UTR of mE-cad" regulatory complement(3660..4167) /note="mPGK1 promoter" /regulatory_class="promoter" regulatory complement(4203..4654) /note="3' UTR of mPGK1, incl. polyA" /regulatory_class="terminator" CDS complement(4679..5473) /transl_table=11 /note="neomycin" /protein_id="SJL88810.1" /translation="MGSADGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" regulatory complement(5486..5993) /note="mPGK1 promoter" /regulatory_class="promoter" regulatory complement(6053..6069) /note="T3 promoter" /regulatory_class="promoter" regulatory complement(6133..6216) /note="lac promoter" /regulatory_class="promoter" rep_origin complement(6308..6699) /note="ColE1 ori" CDS complement(7240..8100) /transl_table=11 /note="ampicillin" /protein_id="SJL88811.1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" BASE COUNT 1937 a 2209 c 2231 g 1922 t ORIGIN 1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc 241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat 301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt 361 tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gcttctgatg gaattagaac 421 ttggcaaaac aatactgaga atgaagtgta tgtggaacag aggctgctga tctcgttctn 481 caggctatga aactgacaca tttggaaacc acagtactta gaaccacaaa gtgggaatca 541 agagaaaaac aatgatccca cgagagatct atagatctat agatcatgag tgggaggaat 601 gagctggccc ttaatttggt tttgcttgtt taaattatga tatccaacta tgaaacatta 661 tcataaagca atagtaaaga gccttcagta aagagcaggc atttatctaa tcccacccca 721 cccccacccc cgtagctcca atccttccat tcaaaatgta ggtactctgt tctcaccctt 781 cttaacaaag tatgacagga aaaacttcca ttttagtgga catctttatt gtttaataga 841 tcatcaattt ctgcaggtcc tggccggggt cccgcggaaa ctcggccgtg gtgaccaata 901 caaaacaaaa gccatggtgc cacacggggg agacttgcta gtcccctagt cgtcctcgcc 961 accgccgtac atgtccgcca gcttcttgaa tcggttgccc cactcgttca gataatcgta 1021 gtcctggtcc tgatccgact cagaggagtt cagtgagctc aggctagcgg cttcagaacc 1081 actgccctcg taatcgaaca ccaacagaga gtcgtaaggg ggtgccgtgg ggtcgctgtc 1141 ggctgccttc aggttttcat cgatgaagtt tccaatttca tcaggattgg caggacgggg 1201 acgatactgg ggcacgctca tgagggtggg agccacatca tttcgagtca cttccggtcg 1261 ggcatccagg cccctgtgca gctggctcaa atcaaagtcc tggtcttctt ctccacctcc 1321 ttcttcatca tagtaataca cattgtcccg ggtatcatca tctggtggca gcaggggctc 1381 tttgaccacc gttctcctcc gtagaaacag taggagcagc aggatcagaa tcagcagggc 1441 gaggatccct ccaaggattc cgaggatggc aggaacttgc aatcctgctg ccacgattcc 1501 cgccttcatg cagttgttga ccgtcccttc acagtcacac acatggacgt ccaacgtggt 1561 cacctggtct ttgttctggt tatccgcgag cttgagatgg attttgtatt cgccaatctc 1621 taagtccttt cttggttgca aaatgagaga ttcttgagct gcgtcattat actcaatggt 1681 ccagttgacg ctggccccat gggttagctc agcagtaaag ggggacgtgt tggggggaag 1741 gtctggatcc aagatggtga tgatatgagg ctgtgggttc ctctggcaga actgcatgtt 1801 tcgaggttct gggatgggag cgttgtcatt gacgtctaac aggaccagga gaagagtgcc 1861 cgtgccagtg gcaatgggtg aaccatcatc tgtggcgatg atgagagcta catatgtgct 1921 gttcttcaca tgctcagcgt cttctctgtc catctcagcg cgcgtgaaaa tggcaccagt 1981 ctctgggtta atctccagcc agttggcagt gtccctccaa atccgatacg tgatcttctg 2041 atccatgaac gtgtccggct ctcgagcggt ataagatgtg atttcctgac ccacaccaaa 2101 gtcttcgggc acttcgactc tcctctccgc aggcataaag atgggggctt cattcacgtc 2161 taccacgtcc acagtgacag tggctgtgga agggacaaga gacccctcaa agggttcctc 2221 gttctccact ctcacatgaa ggatgtattg ctgcttggcc tcaaaatcca agcccttggc 2281 tgttttcaaa atgccatcat tggtcgtggg gtctgtgacg acaacgaact gctggtcagg 2341 atcgttgact acggtgtaca cagctttcca cgccggagtg ttgggggcat catcatcggt 2401 cactttgagt gtggcgatcc gggcattgac ctcattctca ggcacttgac cctgatacgt 2461 gctcgggttg aagacaggag cgttgtcatt aatatccttg acagtgatca cagccttggc 2521 tgttgtgctc aagccttcgc cttgaaggtc agcagcctga accaccagag tgtatgtagg 2581 gtaactctct cggtccagcc cagaggtgag cacactgatg accccggtgt ccctattgac 2641 agtgaacatg tttttgtgag gcagctcagg atcctggctg acgatggtgt aggcgatggc 2701 agcgttgtag gtgttgacgt catcgtctgc atcggtggct gagaccttca tcacggaggt 2761 tcctggaaca gcgccttctg caacggatcc ctcaaacacc tcctgggtaa actctggcct 2821 gttgtcattc tgatctgtca ctgtgatcac tatctccatg ggatcctcca ccgcttcccc 2881 atttgatgac acggcatgag aatagaggat gtacttggca atggcttctc tatccagagg 2941 ctgtgtcact ttcagccagc ctgtctccct ctcaatgatg aaaacgccaa cggggggttt 3001 gtcagctcct tggccggtga tgctgtagaa aacctttgtt tctttgtccc tgttggattt 3061 gatctgaacc aggttctttg ggaattcacc cttttcattt tcggggcagc tgatgggagg 3121 gatgacccag tctcgtttct gtcttctgag acctgggtac acgctgggaa acatgagcag 3181 ctctgggttg gattcagagg cagggtcgcg gtggtggtgc cggtgatggt ggtgccccat 3241 ggacttcagc gtcactttgg tagacagctc cctatgactg gagtcccggg cgcggacgag 3301 gaaactggtc tccagcttgt ggagctttag atgccgcttc actgtgatgg tgccgtctgt 3361 cgccactttg aatcgggagt cttccgaaaa gaaggctgtc cttggccggc cggtgcatcc 3421 ttcaaatctc actctgccca ggacatggcc tctctccagg tgcctctccg gcaccgggaa 3481 ggtgtagacc tcggaactga agccgggact gcaggactca ggctccagct cctggcaaag 3541 ccatgaggag acctgcagca ggagcaggag cgcggaaaag ctgcggcacc gggctcccat 3601 ggcggacccg gcagacgccg agcaaacact gaggacgctg ttaagcgggt cgctgcaggt 3661 cgaaaggccc ggagatgagg aagaggagaa cagcgcggca gacgtgcgct tttgaagcgt 3721 gcagaatgcc gggcctccgg aggaccttcg ggcgcccgcc ccgcccctga gcccgcccct 3781 gagcccgccc ccggacccac cccttcccag cctctgagcc cagaaagcga aggagcaaag 3841 ctgctattgg ccgctgcccc aaaggcctac ccgcttccat tgctcagcgg tgctgtccat 3901 ctgcacgaga ctagtgagac gtgctacttc catttgtcac gtcctgcacg acgcgagctg 3961 cggggcgggg gggaacttcc tgactagggg aggagtagaa ggtggcgcga aggggccacc 4021 aaagaacgga gccggttggc gcctaccggt ggatgtggaa tgtgtgcgag gccagaggcc 4081 acttgtgtag cgccaagtgc ccagcggggc tgctaaagcg catgctccag actgccttgg 4141 gaaaagcgcc tcccctaccc ggtagaattc gagctcggta cccggggatc ctctagagtc 4201 agcttctgat ggaattagaa cttggcaaaa caatactgag aatgaagtgt atgtggaaca 4261 gaggctgctg atctcgttct tcaggctatg aaactgacac atttggaaac cacagtactt 4321 agaaccacaa agtgggaatc aagagaaaaa caatgatccc acgagagatc tatagatcta 4381 tagatcatga gtgggaggaa tgagctggcc cttaatttgg ttttgcttgt ttaaattatg 4441 atatccaact atgaaacatt atcataaagc aatagtaaag agccttcagt aaagagcagg 4501 catttatcta atcccacccc acccccaccc ccgtagctcc aatccttcca ttcaaaatgt 4561 aggtactctg ttctcaccct tcttaacaaa gtatgacagg aaaaacttcc attttagtgg 4621 acatctttat tgtttaatag atcatcaatt tctgcagact tacagcggat cgatcccctc 4681 agaagaactc gtcaagaagg cgatagaagg cgatgcgctg cgaatcggga gcggcgatac 4741 cgtaaagcac gaggaagcgg tcagcccatt cgccgccaag ctcttcagca atatcacggg 4801 tagccaacgc tatgtcctga tagcggtccg ccacacccag ccggccacag tcgatgaatc 4861 cagaaaagcg gccattttcc accatgatat tcggcaagca ggcatcgcca tgggtcacga 4921 cgagatcctc gccgtcgggc atgcgcgcct tgagcctggc gaacagttcg gctggcgcga 4981 gcccctgatg ctcttcgtcc agatcatcct gatcgacaag accggcttcc atccgagtac 5041 gtgctcgctc gatgcgatgt ttcgcttggt ggtcgaatgg gcaggtagcc ggatcaagcg 5101 tatgcagccg ccgcattgca tcagccatga tggatacttt ctcggcagga gcaaggtgag 5161 atgacaggag atcctgcccc ggcacttcgc ccaatagcag ccagtccctt cccgcttcag 5221 tgacaacgtc gagcacagct gcgcaaggaa cgcccgtcgt ggccagccac gatagccgcg 5281 ctgcctcgtc ctgcagttca ttcagggcac cggacaggtc ggtcttgaca aaaagaaccg 5341 ggcgcccctg cgctgacagc cggaacacgg cggcatcaga gcagccgatt gtctgttgtg 5401 cccagtcata gccgaatagc ctctccaccc aagcggccgg agaacctgcg tgcaatccat 5461 cggccgatcc catattggct gcaggtcgaa aggcccggag atgaggaaga ggagaacagc 5521 gcggcagacg tgcgcttttg aagcgtgcag aatgccgggc ctccggagga ccttcgggcg 5581 cccgccccgc ccctgagccc gcccctgagc ccgcccccgg acccacccct tcccagcctc 5641 tgagcccaga aagcgaagga gcaaagctgc tattggccgc tgccccaaag gcctacccgc 5701 ttccattgct cagcggtgct gtccatctgc acgagactag tgagacgtgc tacttccatt 5761 tgtcacgtcc tgcacgacgc gagctgcggg gcggggggga acttcctgac taggggagga 5821 gtagaaggtg gcgcgaaggg gccaccaaag aacggagccg gttggcgcct accggtggat 5881 gtggaatgtg tgcgaggcca gaggccactt gtgtagcgcc aagtgcccag cggggctgct 5941 aaagcgcatg ctccagactg ccttgggaaa agcgcctccc ctacccggta gaattgacct 6001 gcaggggccc tcgagcggcc gccaccgcgg tggagctcca gcttttgttc cctttagtga 6061 gggttaattg cgcgcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat 6121 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc 6181 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga 6241 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt 6301 attgggcgct tttccgcttc ctcgctcact gactcgctac gctcggtcgt tcgactgcgg 6361 cgagcggtac tgactcacac aaaaacggta acacagttat ccacagaatc aggggataag 6421 gccggaaaga acatgtgagc aaaagaccag gaacaggaag aaggccacgt agcaggcgtt 6481 tttccatagg ctccgccccc ctgacgagca tcacaaaaat agacgctcaa gtcagaggtg 6541 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg 6601 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag 6661 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc 6721 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa 6781 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg 6841 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc 6901 taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac 6961 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg 7021 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt 7081 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt 7141 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa 7201 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga 7261 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt 7321 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg 7381 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga 7441 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga 7501 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg 7561 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc 7621 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc 7681 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca 7741 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac 7801 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg 7861 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc 7921 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg 7981 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac 8041 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat 8101 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata 8161 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa 8221 agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg 8281 tatcacgagg ccctttcgtc //