LOCUS       LT727548                8300 bp    DNA     circular SYN 06-FEB-2017
DEFINITION  Vector pPNEcad, complete sequence.
ACCESSION   LT727548
VERSION     LT727548.1
KEYWORDS    .
SOURCE      Vector pPNEcad
  ORGANISM  Vector pPNEcad
            other sequences; artificial sequences; vectors.
REFERENCE   1  (bases 1 to 8300)
  AUTHORS   De Schamphelaire W.
  JOURNAL   Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
            Technologiepark 927, 9052, BELGIUM.
REFERENCE   2
  AUTHORS   De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
            Roggeman Fonseca M., Vanhoucke M., Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished.
FEATURES             Location/Qualifiers
     source          1..8300
                     /organism="Vector pPNEcad"
                     /mol_type="other DNA"
                     /note="BCCM/LMBP Plasmid collection (Ghent
                     University,Belgium) accession number LMBP 2828."
                     /db_xref="taxon:1945367"
     regulatory      complement(402..853)
                     /note="3' UTR of mPGK1, incl. polyA"
                     /regulatory_class="terminator"
     misc_feature    complement(912..945)
                     /note="3' UTR of mE-cad"
     CDS             complement(946..3600)
                     /transl_table=11
                     /note="mE-cad"
                     /protein_id="SJL88809.1"
                     /translation="MGARCRSFSALLLLLQVSSWLCQELEPESCSPGFSSEVYTFPVP
                     ERHLERGHVLGRVRFEGCTGRPRTAFFSEDSRFKVATDGTITVKRHLKLHKLETSFLV
                     RARDSSHRELSTKVTLKSMGHHHHRHHHRDPASESNPELLMFPSVYPGLRRQKRDWVI
                     PPISCPENEKGEFPKNLVQIKSNRDKETKVFYSITGQGADKPPVGVFIIERETGWLKV
                     TQPLDREAIAKYILYSHAVSSNGEAVEDPMEIVITVTDQNDNRPEFTQEVFEGSVAEG
                     AVPGTSVMKVSATDADDDVNTYNAAIAYTIVSQDPELPHKNMFTVNRDTGVISVLTSG
                     LDRESYPTYTLVVQAADLQGEGLSTTAKAVITVKDINDNAPVFNPSTYQGQVPENEVN
                     ARIATLKVTDDDAPNTPAWKAVYTVVNDPDQQFVVVTDPTTNDGILKTAKGLDFEAKQ
                     QYILHVRVENEEPFEGSLVPSTATVTVDVVDVNEAPIFMPAERRVEVPEDFGVGQEIT
                     SYTAREPDTFMDQKITYRIWRDTANWLEINPETGAIFTRAEMDREDAEHVKNSTYVAL
                     IIATDDGSPIATGTGTLLLVLLDVNDNAPIPEPRNMQFCQRNPQPHIITILDPDLPPN
                     TSPFTAELTHGASVNWTIEYNDAAQESLILQPRKDLEIGEYKIHLKLADNQNKDQVTT
                     LDVHVCDCEGTVNNCMKAGIVAAGLQVPAILGILGGILALLILILLLLLFLRRRTVVK
                     EPLLPPDDDTRDNVYYYDEEGGGEEDQDFDLSQLHRGLDARPEVTRNDVAPTLMSVPQ
                     YRPRPANPDEIGNFIDENLKAADSDPTAPPYDSLLVFDYEGSGSEAASLSSLNSSESD
                     QDQDYDYLNEWGNRFKKLADMYGGGEDD"
     misc_feature    complement(3601..3633)
                     /note="5' UTR of mE-cad"
     regulatory      complement(3660..4167)
                     /note="mPGK1 promoter"
                     /regulatory_class="promoter"
     regulatory      complement(4203..4654)
                     /note="3' UTR of mPGK1, incl. polyA"
                     /regulatory_class="terminator"
     CDS             complement(4679..5473)
                     /transl_table=11
                     /note="neomycin"
                     /protein_id="SJL88810.1"
                     /translation="MGSADGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
                     LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY
                     QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     regulatory      complement(5486..5993)
                     /note="mPGK1 promoter"
                     /regulatory_class="promoter"
     regulatory      complement(6053..6069)
                     /note="T3 promoter"
                     /regulatory_class="promoter"
     regulatory      complement(6133..6216)
                     /note="lac promoter"
                     /regulatory_class="promoter"
     rep_origin      complement(6308..6699)
                     /note="ColE1 ori"
     CDS             complement(7240..8100)
                     /transl_table=11
                     /note="ampicillin"
                     /protein_id="SJL88811.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
BASE COUNT         1937 a         2209 c         2231 g         1922 t
ORIGIN      
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gcttctgatg gaattagaac
      421 ttggcaaaac aatactgaga atgaagtgta tgtggaacag aggctgctga tctcgttctn
      481 caggctatga aactgacaca tttggaaacc acagtactta gaaccacaaa gtgggaatca
      541 agagaaaaac aatgatccca cgagagatct atagatctat agatcatgag tgggaggaat
      601 gagctggccc ttaatttggt tttgcttgtt taaattatga tatccaacta tgaaacatta
      661 tcataaagca atagtaaaga gccttcagta aagagcaggc atttatctaa tcccacccca
      721 cccccacccc cgtagctcca atccttccat tcaaaatgta ggtactctgt tctcaccctt
      781 cttaacaaag tatgacagga aaaacttcca ttttagtgga catctttatt gtttaataga
      841 tcatcaattt ctgcaggtcc tggccggggt cccgcggaaa ctcggccgtg gtgaccaata
      901 caaaacaaaa gccatggtgc cacacggggg agacttgcta gtcccctagt cgtcctcgcc
      961 accgccgtac atgtccgcca gcttcttgaa tcggttgccc cactcgttca gataatcgta
     1021 gtcctggtcc tgatccgact cagaggagtt cagtgagctc aggctagcgg cttcagaacc
     1081 actgccctcg taatcgaaca ccaacagaga gtcgtaaggg ggtgccgtgg ggtcgctgtc
     1141 ggctgccttc aggttttcat cgatgaagtt tccaatttca tcaggattgg caggacgggg
     1201 acgatactgg ggcacgctca tgagggtggg agccacatca tttcgagtca cttccggtcg
     1261 ggcatccagg cccctgtgca gctggctcaa atcaaagtcc tggtcttctt ctccacctcc
     1321 ttcttcatca tagtaataca cattgtcccg ggtatcatca tctggtggca gcaggggctc
     1381 tttgaccacc gttctcctcc gtagaaacag taggagcagc aggatcagaa tcagcagggc
     1441 gaggatccct ccaaggattc cgaggatggc aggaacttgc aatcctgctg ccacgattcc
     1501 cgccttcatg cagttgttga ccgtcccttc acagtcacac acatggacgt ccaacgtggt
     1561 cacctggtct ttgttctggt tatccgcgag cttgagatgg attttgtatt cgccaatctc
     1621 taagtccttt cttggttgca aaatgagaga ttcttgagct gcgtcattat actcaatggt
     1681 ccagttgacg ctggccccat gggttagctc agcagtaaag ggggacgtgt tggggggaag
     1741 gtctggatcc aagatggtga tgatatgagg ctgtgggttc ctctggcaga actgcatgtt
     1801 tcgaggttct gggatgggag cgttgtcatt gacgtctaac aggaccagga gaagagtgcc
     1861 cgtgccagtg gcaatgggtg aaccatcatc tgtggcgatg atgagagcta catatgtgct
     1921 gttcttcaca tgctcagcgt cttctctgtc catctcagcg cgcgtgaaaa tggcaccagt
     1981 ctctgggtta atctccagcc agttggcagt gtccctccaa atccgatacg tgatcttctg
     2041 atccatgaac gtgtccggct ctcgagcggt ataagatgtg atttcctgac ccacaccaaa
     2101 gtcttcgggc acttcgactc tcctctccgc aggcataaag atgggggctt cattcacgtc
     2161 taccacgtcc acagtgacag tggctgtgga agggacaaga gacccctcaa agggttcctc
     2221 gttctccact ctcacatgaa ggatgtattg ctgcttggcc tcaaaatcca agcccttggc
     2281 tgttttcaaa atgccatcat tggtcgtggg gtctgtgacg acaacgaact gctggtcagg
     2341 atcgttgact acggtgtaca cagctttcca cgccggagtg ttgggggcat catcatcggt
     2401 cactttgagt gtggcgatcc gggcattgac ctcattctca ggcacttgac cctgatacgt
     2461 gctcgggttg aagacaggag cgttgtcatt aatatccttg acagtgatca cagccttggc
     2521 tgttgtgctc aagccttcgc cttgaaggtc agcagcctga accaccagag tgtatgtagg
     2581 gtaactctct cggtccagcc cagaggtgag cacactgatg accccggtgt ccctattgac
     2641 agtgaacatg tttttgtgag gcagctcagg atcctggctg acgatggtgt aggcgatggc
     2701 agcgttgtag gtgttgacgt catcgtctgc atcggtggct gagaccttca tcacggaggt
     2761 tcctggaaca gcgccttctg caacggatcc ctcaaacacc tcctgggtaa actctggcct
     2821 gttgtcattc tgatctgtca ctgtgatcac tatctccatg ggatcctcca ccgcttcccc
     2881 atttgatgac acggcatgag aatagaggat gtacttggca atggcttctc tatccagagg
     2941 ctgtgtcact ttcagccagc ctgtctccct ctcaatgatg aaaacgccaa cggggggttt
     3001 gtcagctcct tggccggtga tgctgtagaa aacctttgtt tctttgtccc tgttggattt
     3061 gatctgaacc aggttctttg ggaattcacc cttttcattt tcggggcagc tgatgggagg
     3121 gatgacccag tctcgtttct gtcttctgag acctgggtac acgctgggaa acatgagcag
     3181 ctctgggttg gattcagagg cagggtcgcg gtggtggtgc cggtgatggt ggtgccccat
     3241 ggacttcagc gtcactttgg tagacagctc cctatgactg gagtcccggg cgcggacgag
     3301 gaaactggtc tccagcttgt ggagctttag atgccgcttc actgtgatgg tgccgtctgt
     3361 cgccactttg aatcgggagt cttccgaaaa gaaggctgtc cttggccggc cggtgcatcc
     3421 ttcaaatctc actctgccca ggacatggcc tctctccagg tgcctctccg gcaccgggaa
     3481 ggtgtagacc tcggaactga agccgggact gcaggactca ggctccagct cctggcaaag
     3541 ccatgaggag acctgcagca ggagcaggag cgcggaaaag ctgcggcacc gggctcccat
     3601 ggcggacccg gcagacgccg agcaaacact gaggacgctg ttaagcgggt cgctgcaggt
     3661 cgaaaggccc ggagatgagg aagaggagaa cagcgcggca gacgtgcgct tttgaagcgt
     3721 gcagaatgcc gggcctccgg aggaccttcg ggcgcccgcc ccgcccctga gcccgcccct
     3781 gagcccgccc ccggacccac cccttcccag cctctgagcc cagaaagcga aggagcaaag
     3841 ctgctattgg ccgctgcccc aaaggcctac ccgcttccat tgctcagcgg tgctgtccat
     3901 ctgcacgaga ctagtgagac gtgctacttc catttgtcac gtcctgcacg acgcgagctg
     3961 cggggcgggg gggaacttcc tgactagggg aggagtagaa ggtggcgcga aggggccacc
     4021 aaagaacgga gccggttggc gcctaccggt ggatgtggaa tgtgtgcgag gccagaggcc
     4081 acttgtgtag cgccaagtgc ccagcggggc tgctaaagcg catgctccag actgccttgg
     4141 gaaaagcgcc tcccctaccc ggtagaattc gagctcggta cccggggatc ctctagagtc
     4201 agcttctgat ggaattagaa cttggcaaaa caatactgag aatgaagtgt atgtggaaca
     4261 gaggctgctg atctcgttct tcaggctatg aaactgacac atttggaaac cacagtactt
     4321 agaaccacaa agtgggaatc aagagaaaaa caatgatccc acgagagatc tatagatcta
     4381 tagatcatga gtgggaggaa tgagctggcc cttaatttgg ttttgcttgt ttaaattatg
     4441 atatccaact atgaaacatt atcataaagc aatagtaaag agccttcagt aaagagcagg
     4501 catttatcta atcccacccc acccccaccc ccgtagctcc aatccttcca ttcaaaatgt
     4561 aggtactctg ttctcaccct tcttaacaaa gtatgacagg aaaaacttcc attttagtgg
     4621 acatctttat tgtttaatag atcatcaatt tctgcagact tacagcggat cgatcccctc
     4681 agaagaactc gtcaagaagg cgatagaagg cgatgcgctg cgaatcggga gcggcgatac
     4741 cgtaaagcac gaggaagcgg tcagcccatt cgccgccaag ctcttcagca atatcacggg
     4801 tagccaacgc tatgtcctga tagcggtccg ccacacccag ccggccacag tcgatgaatc
     4861 cagaaaagcg gccattttcc accatgatat tcggcaagca ggcatcgcca tgggtcacga
     4921 cgagatcctc gccgtcgggc atgcgcgcct tgagcctggc gaacagttcg gctggcgcga
     4981 gcccctgatg ctcttcgtcc agatcatcct gatcgacaag accggcttcc atccgagtac
     5041 gtgctcgctc gatgcgatgt ttcgcttggt ggtcgaatgg gcaggtagcc ggatcaagcg
     5101 tatgcagccg ccgcattgca tcagccatga tggatacttt ctcggcagga gcaaggtgag
     5161 atgacaggag atcctgcccc ggcacttcgc ccaatagcag ccagtccctt cccgcttcag
     5221 tgacaacgtc gagcacagct gcgcaaggaa cgcccgtcgt ggccagccac gatagccgcg
     5281 ctgcctcgtc ctgcagttca ttcagggcac cggacaggtc ggtcttgaca aaaagaaccg
     5341 ggcgcccctg cgctgacagc cggaacacgg cggcatcaga gcagccgatt gtctgttgtg
     5401 cccagtcata gccgaatagc ctctccaccc aagcggccgg agaacctgcg tgcaatccat
     5461 cggccgatcc catattggct gcaggtcgaa aggcccggag atgaggaaga ggagaacagc
     5521 gcggcagacg tgcgcttttg aagcgtgcag aatgccgggc ctccggagga ccttcgggcg
     5581 cccgccccgc ccctgagccc gcccctgagc ccgcccccgg acccacccct tcccagcctc
     5641 tgagcccaga aagcgaagga gcaaagctgc tattggccgc tgccccaaag gcctacccgc
     5701 ttccattgct cagcggtgct gtccatctgc acgagactag tgagacgtgc tacttccatt
     5761 tgtcacgtcc tgcacgacgc gagctgcggg gcggggggga acttcctgac taggggagga
     5821 gtagaaggtg gcgcgaaggg gccaccaaag aacggagccg gttggcgcct accggtggat
     5881 gtggaatgtg tgcgaggcca gaggccactt gtgtagcgcc aagtgcccag cggggctgct
     5941 aaagcgcatg ctccagactg ccttgggaaa agcgcctccc ctacccggta gaattgacct
     6001 gcaggggccc tcgagcggcc gccaccgcgg tggagctcca gcttttgttc cctttagtga
     6061 gggttaattg cgcgcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat
     6121 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc
     6181 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
     6241 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
     6301 attgggcgct tttccgcttc ctcgctcact gactcgctac gctcggtcgt tcgactgcgg
     6361 cgagcggtac tgactcacac aaaaacggta acacagttat ccacagaatc aggggataag
     6421 gccggaaaga acatgtgagc aaaagaccag gaacaggaag aaggccacgt agcaggcgtt
     6481 tttccatagg ctccgccccc ctgacgagca tcacaaaaat agacgctcaa gtcagaggtg
     6541 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     6601 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     6661 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     6721 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     6781 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     6841 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     6901 taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac
     6961 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     7021 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     7081 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     7141 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     7201 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     7261 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     7321 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     7381 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     7441 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     7501 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     7561 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     7621 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     7681 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     7741 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     7801 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     7861 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     7921 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     7981 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     8041 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     8101 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     8161 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     8221 agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg
     8281 tatcacgagg ccctttcgtc
//