LOCUS LT727546 4417 bp DNA circular SYN 06-FEB-2017
DEFINITION Vector pSP64GSHIFNBNT, complete sequence.
ACCESSION LT727546
VERSION LT727546.1
KEYWORDS .
SOURCE Vector pSP64GSHIFNBNT
ORGANISM Vector pSP64GSHIFNBNT
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 4417)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..4417
/organism="Vector pSP64GSHIFNBNT"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 2789."
/db_xref="taxon:1945399"
misc_feature 11..53
/note="5' UTR of beta-globin"
CDS 90..653
/transl_table=11
/note="hIFNbeta"
/protein_id="SJL88806.1"
/translation="MTNKCLLQIALLLCFSTTALSMSYNLLGFLQRSSNFQCQKLLWQ
LNGRLEYCLKDRMNFDIPEEIKQLQQFQKEDAALTIYEMLQNIFAIFRQDSSSTGWNE
TIVENLLANVYHQINHLKTVLEEKLEKEDFTRGKLMSSLHLKRYYGRILHYLKAKEYS
HCAWTIVRVEILRNFYFINRLTGYLRN"
misc_feature 654..853
/note="3' UTR of hIFNbeta"
misc_feature 910..1050
/note="3' UTR of beta-globin"
regulatory 1114..1445
/note="hIFNalpha14 terminator"
/regulatory_class="terminator"
regulatory complement(1522..1605)
/note="lac promoter"
/regulatory_class="promoter"
rep_origin complement(1697..2099)
/note="pMB1 ori"
CDS complement(2640..3500)
/transl_table=11
/note="ampicillin"
/protein_id="SJL88807.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
regulatory 4395..4411
/note="SP6 promoter"
/regulatory_class="promoter"
BASE COUNT 1185 a 1094 c 1009 g 1129 t
ORIGIN
1 aagctagctt cttgttcttt ttgcagaagc tcagaataaa cgctcaactt tggcagatcc
61 caagcttggc caaaaaggcc tcgaggaaca tgaccaacaa gtgtctcctc caaattgctc
121 tcctgttgtg cttctccact acagctcttt ccatgagcta caacttgctt ggattcctac
181 aaagaagcag caattttcag tgtcagaagc tcctgtggca attgaatggg aggcttgaat
241 actgcctcaa ggacaggatg aactttgaca tccctgagga gattaagcag ctgcagcagt
301 tccagaagga ggacgccgca ttgaccatct atgagatgct ccagaacatc tttgctattt
361 tcagacaaga ttcatctagc actggctgga atgagactat tgttgagaac ctcctggcta
421 atgtctatca tcagataaac catctgaaga cagtcctgga agaaaaactg gagaaagaag
481 atttcaccag gggaaaactc atgagcagtc tgcacctgaa aagatattat gggaggattc
541 tgcattacct gaaggccaag gagtacagtc actgtgcctg gaccatagtc agagtggaaa
601 tcctaaggaa cttttacttc attaacagac ttacaggtta cctccgaaac tgaagatctc
661 ctagcctgtg cctctgggac tggacaattg cttcaagcat tcttcaacca gcagatgctg
721 tttaagtgac tgatggctaa tgtactgcat atgaaaggac actagaagat tttgaaattt
781 ttattaaatt atgagttatt tttatttatt taaattttat tttggaaaat aaattatttt
841 tggtgcaaaa gtccctcgag gcctagcggc cgcctagagg atccccgggc gagctcgaat
901 tcagatctgg ttaccactaa accagcctca agaacacccg aatggagtct ctaagctaca
961 taataccaac ttacacttta caaaatgttg tcccccaaaa tgtagccatt cgtatctgct
1021 cctaataaaa agaaagtttc ttcacattct aaaaaaaaaa aaaaaaaaaa aaaccccccc
1081 cccccccccc cccccccccc cccgatcccc ggggatccct ctctttttca acaaacttgc
1141 aaaaaagatt aaggaggaag gattgaaaac tggttcatca tggaaatgat tctcattgac
1201 taatacatca tctcacactt tcatgagttc ttccatttca aagactcact tctcctataa
1261 ccaccacaag ttgaatcaaa attttcaaat gttttcagga gtgtaaagaa gcatcatgta
1321 tacctgtgca ggcactagtc ctttacagat gaccatgctg atgtctcctt tcatctattt
1381 atttaaatat ttatttattt aactattttt attatttaaa ttatttttta tgtaatatca
1441 tgtgtgacct gcagcccaag ctcgaattcg taatcatggt catagctgtt tcctgtgtga
1501 aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc
1561 tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc
1621 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc
1681 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
1741 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
1801 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
1861 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
1921 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
1981 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
2041 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
2101 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
2161 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
2221 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
2281 gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc
2341 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
2401 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
2461 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
2521 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
2581 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
2641 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
2701 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
2761 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
2821 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
2881 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
2941 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
3001 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
3061 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
3121 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
3181 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
3241 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
3301 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
3361 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
3421 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
3481 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
3541 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
3601 ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca
3661 ttaacctata aaaataggcg tatcacgagg ccctttcgtc tcgcgcgttt cggtgatgac
3721 ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat
3781 gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg
3841 cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatcga cgctctccct
3901 tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg
3961 ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg
4021 ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc
4081 catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg
4141 ccacgatgcg tccggcgtag aggatctggc tagcgatgac cctgctgatt ggttcgctga
4201 ccatttccgg ggtgcggaac ggcgttacca gaaactcaga aggttcgtcc aaccaaaccg
4261 actctgacgg cagtttacga gagagatgat agggtctgct tcagtaagcc agatgctaca
4321 caattaggct tgtacatatt gtcgttagaa cgcggctaca attaatacat aaccttatgt
4381 atcatacaca tacgatttag gtgacactat agaatac
//