LOCUS LT727542 9104 bp DNA circular SYN 06-FEB-2017
DEFINITION Mammalian expression vector pBact-Pcad, complete sequence.
ACCESSION LT727542
VERSION LT727542.1
KEYWORDS .
SOURCE Mammalian expression vector pBact-Pcad
ORGANISM Mammalian expression vector pBact-Pcad
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 9104)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..9104
/organism="Mammalian expression vector pBact-Pcad"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 2766."
/db_xref="taxon:1944749"
regulatory 341..2471
/note="Ac promoter"
/regulatory_class="promoter"
misc_feature 2472..3466
/note="5' UTR of chicken beta-actin"
misc_feature 3512..3556
/note="5' UTR of mCdh3"
CDS 3557..6025
/transl_table=11
/note="mCdh3"
/protein_id="SJL88797.1"
/translation="MELLSGPHAFLLLLLQVCWLRSVVSEPYRAGFIGEAGVTLEVEG
TDLEPSQVLGKVALAGQGMHHADNGDIIMLTRGTVQGGKDAMHSPPTRILRRRKREWV
MPPIFVPENGKGPFPQRLNQLKSNKDRGTKIFYSITGPGADSPPEGVFTIEKESGWLL
LHMPLDREKIVKYELYGHAVSENGASVEEPMNISIIVTDQNDNKPKFTQDTFRGSVLE
GVMPGTSVMQVTATDEDDAVNTYNGVVAYSIHSQEPKEPHDLMFTIHKSTGTISVISS
GLDREKVPEYRLTVQATDMDGEGSTTTAEAVVQILDANDNAPEFEPQKYEAWVPENEV
GHEVQRLTVTDLDVPNSPAWRATYHIVGGDDGDHFTITTHPETNQGVLTTKKGLDFEA
QDQHTLYVEVTNEAPFAVKLPTATATVVVHVKDVNEAPVFVPPSKVIEAQEGISIGEL
VCIYTAQDPDKEDQKISYTISRDPANWLAVDPDSGQITAAGILDREDEQFVKNNVYEV
MVLATDSGNPPTTGTGTLLLTLTDINDHGPIPEPRQIIICNQSPVPQVLNITDKDLSP
NSSPFQAQLTHDSDIYWMAEVSEKGDTVALSLKKFLKQDTYDLHLSLSDHGNREQLTM
IRATVCDCHGQVFNDCPRPWKGGFILPILGAVLALLTLLLALLLLVRKKRKVKEPLLL
PEDDTRDNVFYYGEEGGGEEDQDYDITQLHRGLEARPEVVLRNDVVPTFIPTPMYRPR
PANPDEIGNFIIENLKAANTDPTAPPYDSLLVFDYEGSGSDAASLSSLTTSASDQDQD
YNYLNEWGSRFKKLADMYGGGEDD"
misc_feature 6026..6698
/note="3' UTR of mCdh3"
regulatory 6717..6791
/note="SV40 polyA early"
/regulatory_class="terminator"
regulatory complement(6717..6791)
/note="SV40 polyA late"
/regulatory_class="terminator"
regulatory complement(6926..7009)
/note="lac promoter"
/regulatory_class="promoter"
rep_origin complement(7101..7503)
/note="pMB1 ori"
CDS complement(8044..8904)
/transl_table=11
/note="ampicillin resistance"
/protein_id="SJL88798.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
BASE COUNT 1944 a 2606 c 2567 g 1978 t
ORIGIN
1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
361 tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gttgggatct ttgcattggc
421 ccacggctct caggatgggg atgctcccct tcagcacccg gttccccttg gaaactgatg
481 gtcctggctc tgtggcatgg cagtggcact gtgaggagcc ccctaccagc agcacacagt
541 ggggttggca ctgccacgct ccggatgccg cgctctgatc caaccccata atcaagggaa
601 cccgaattgc cccatcattg cccccaccac ccccatcctg ccgggccctc acaccccacg
661 ctgccttctg gtgacattcc ccagcccaaa cccacggctt catggctacc gcggggcatt
721 tcccattgcc gccccattat cagctctgca cacctcccgc tgtacccatg cctcgtggct
781 gcccttcttt gacgtataat cttctaatta atacccggcc ttgtcaaagt ggagcacaaa
841 cgttaattaa ttccccagca ggcaggtaat taacagtgtg actccctttt tgctgcgagt
901 ggggctgata cagagagatg tggcactatg gagcccacgg ggtcctggca ctgggtgccc
961 acggaggtcc ccatgtgctg cagtgtcacc gcctccgagg agacagtatt gtccctgcgg
1021 tgtccctgca gctcagctct gtccacaggg ccacctccag tttggagggg acacaatgca
1081 gccccgatgc aacccatcct cgcagcatcc cagggacaaa gaccccactg caagaccgca
1141 cacagggctg ggtcccgctc ccctaatatc tacagtgctt cttgcatggc cccttaatca
1201 atgcagttaa tcagcatgcg ctcatgcacc gctctggagc tgcaaagccc ctcgcagcgc
1261 tgctcaccaa caccgcgcac cgcccctgcc cagcctgcag cacgcgctgc aaacaggaaa
1321 gaaacaaaat attgcccaaa tgtaggcaaa ggcattcggc tgccttgacc tccgccgggc
1381 cgggccctgc ctgactcagc tccttactca gcgctcgctt cctccctccg gctgccaccg
1441 ccgcagcgca caccctgaca aagagtggcc cttaacgggc tctgaggtgc acccagcagt
1501 gcactcagca gtccaagggc cggcctggag gtttgcaccg ctacgtgctg acattagcat
1561 tgaacttggc cctgggtagt gctgcaggcc gggcggggtg ggtgtagaga gtgcagcgcg
1621 cgttgcaccc ggtgcccctt cccctccctt gcatcccagc aggctgcacc ccagcaccag
1681 gcccgtgcat gcatgctcct ggtgttattg cagcctggtg catgcatgcg tcttgatggt
1741 gcagcgctgt gcatgcatcc tccttggtgt gtagcagtct agtgcatgca tacccctcgg
1801 tgttattgct gctctgtgca cgcacgctca ttgtatcact tcatcccagt gcatgcactc
1861 acactggagc gattgctgct cggtgcacgc acactcattg tatcactgca gctcagtggc
1921 tgcacgcaca ccggtgttat tgctgctcgg tgcgtgcatg cacatcagtg tcgctgcagc
1981 tcagtgcatg cacgctcatt gcccatcgct atccctgcct ctcctgctgg cgctccccgg
2041 gaggtgactt caaggggacc gcaggaccac ctcgggggtg gggggagggc tgcacacgcg
2101 gaccccgctc cccctcccca acaaagcact gtggaatcaa aaagggggga ggggggatgg
2161 aggggcgcgt cacacccccg ccccacaccc tcacctcgag gtgagcccca cgttctgctt
2221 cactctcccc atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt
2281 attttgtgca gcgatggggg cggggggggg gggggcgcgc gccaggcggg gcggggcggg
2341 gcgaggggcg gggcggggcg aggcggagag gtgcggcggc agccaatcag agcggcgcgc
2401 tccgaaagtt tccttttatg gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg
2461 cgcggcgggc gggagtcgct gcgttgcctt cgccccgtgc cccgctccgc gccgcctcgc
2521 gccgcccgcc ccggctctga ctgaccgcgt tactcccaca ggtgagcggg cgggacggcc
2581 cttctcctcc gggctgtaat tagcgcttgg tttaatgacg gctcgtttct tttctgtggc
2641 tgcgtgaaag ccttaaaggg ctccgggagg gccctttgtg cgggggggag cggctcgggg
2701 ggtgcgtgcg tgtgtgtgtg cgtggggagc gccgcgtgcg gcccgcgctg cccggcggct
2761 gtgagcgctg cgggcgcggc gcggggcttt gtgcgctccg cgtgtgcgcg aggggagcgc
2821 ggccgggggc ggtgccccgc ggtgcggggg ggctgcgagg ggaacaaagg ctgcgtgcgg
2881 ggtgtgtgcg tgggggggtg agcagggggt gtgggcgcgg cggtcgggct gtaacccccc
2941 cctgcacccc cctccccgag ttgctgagca cggcccggct tcgggtgcgg ggctccgtgc
3001 ggggcgtggc gcggggctcg ccgtgccggg cggggggtgg cggcaggtgg gggtgccggg
3061 cggggcgggg ccgcctcggg ccggggaggg ctcgggggag gggcgcggcg gccccggagc
3121 gccggcggct gtcgaggcgc ggcgagccgc agccattgcc ttttatggta atcgtgcgag
3181 agggcgcagg gacttccttt gtcccaaatc tggcggagcc gaaatctggg aggcgccgcc
3241 gcaccccctc tagcgggcgc gggcgaagcg gtgcggcgcc ggcaggaagg aaatgggcgg
3301 ggagggcctt cgtgcgtcgc cgcgccgccg tccccttctc catctccagc ctcggggctg
3361 ccgcaggggg acggctgcct tcggggggga cggggcaggg cggggttcgg cttctggcgt
3421 gtgaccggcg gggtttatat cttcccttct ctgttcctcc gcagccngtc gactctagag
3481 gatctnnnnn aagcttgata tcgaattcnn ncgagcgcgc cccaccgtcg cggcaccttc
3541 tcacctccct tcagttatgg agcttcttag tgggcctcac gccttcctgc tcctcctgct
3601 ccaggtttgc tggctacgca gcgtggtctc cgagccctac cgagcgggct tcatcgggga
3661 ggctggagtg accttggagg tggaaggaac tgacctggag ccgagccaag ttctggggaa
3721 agtagccttg gctggacagg gcatgcacca tgcagacaat ggagacatca ttatgctgac
3781 tagggggaca gttcagggag ggaaggatgc gatgcactcc ccacccaccc gcatcttaag
3841 gagacgaaag agagagtggg tgatgccacc aatattcgtc cccgagaatg gcaagggtcc
3901 cttccctcag aggctgaatc agctcaaatc taataaggac agaggcacca agattttcta
3961 cagcatcaca gggcctggcg cagacagtcc ccccgaagga gtcttcacca tagagaagga
4021 gtcgggctgg ctgttgttgc atatgccact ggacagggag aagattgtca agtacgagct
4081 ttatggccac gctgtatctg agaatggtgc ctctgtagag gagcccatga acatatccat
4141 cattgtgaca gaccagaatg acaacaagcc caagttcact caagacacct tcagagggag
4201 tgttctggag ggagtaatgc ctggcacttc tgtgatgcag gtgacagcca cagatgagga
4261 cgatgctgtc aacacttaca atggggtggt ggcttactcc atccatagcc aagagccgaa
4321 ggagccacac gacctcatgt tcaccatcca taaaagcacg ggaaccatta gcgtcatatc
4381 cagtggcctg gaccgagaga aagtccctga gtacagactg accgtccagg ccacagacat
4441 ggatggagag ggctctacca cgacggcaga ggccgttgtg caaatccttg atgccaacga
4501 taacgctccg gagtttgagc cgcagaagta tgaggcttgg gtgcctgaga acgaagtggg
4561 ccatgaggta cagaggctga cagtgactga tctcgatgtc cccaactcgc cagcgtggcg
4621 tgccacctac cacatcgtgg gaggtgatga tggggaccat ttcaccatca ccactcaccc
4681 agagaccaac caaggcgtcc tgacaaccaa gaagggtttg gattttgagg ctcaggacca
4741 acacaccctg tatgtagaag tgaccaacga ggctcccttt gcagtgaagc tcccgacagc
4801 cactgccacc gtggtggtcc atgtgaaaga tgtcaacgaa gcccctgtgt ttgttccacc
4861 ttccaaggtc attgaggccc aggaaggcat ctctattggg gaactggtct gcatctatac
4921 cgcacaggac ccagacaagg aggaccagaa gatcagctac accatctcga gagatccagc
4981 caactggctt gctgtggacc cagacagtgg tcagataact gccgcaggca tcttggatcg
5041 tgaggacgag cagtttgtga aaaacaatgt ctacgaagtc atggttttgg ccacagacag
5101 tggaaaccct cccaccaccg gcactgggac cctcctgctt acacttactg acatcaacga
5161 ccatggcccg atccctgaac ccaggcagat catcatctgt aaccaaagcc ctgtgcctca
5221 agtgctgaac atcactgaca aggacctgtc ccccaactcc tcccctttcc aggcccagct
5281 aacacatgac tcagatatct actggatggc agaagtcagc gagaaaggag acaccgtggc
5341 cttgtccctg aagaagttcc tgaaacaaga cacgtatgac ttgcatcttt ctttgtctga
5401 ccatggcaac agggaacagc taaccatgat cagggccact gtgtgtgact gccatggcca
5461 agtgttcaat gactgcccca gaccctggaa gggtggtttc atcctcccca tcctgggtgc
5521 tgtcctggca ctgctgaccc ttctactggc actcctcctg ttggtgagga agaagaggaa
5581 ggtcaaagag ccccttctgc tcccagaaga tgacacgcga gacaatgtct tctattatgg
5641 agaagagggt ggtggtgaag aggaccagga ctatgacatc acccaactcc accggggact
5701 ggaggccagg cctgaggtgg ttctccgaaa cgatgtagtg ccaaccttca tccccacccc
5761 catgtaccga ccccggcccg ccaacccaga tgaaatcggg aacttcatca tcgagaacct
5821 gaaggctgcc aacactgacc ctactgcccc gccctacgac tccctgctgg tttttgacta
5881 cgagggcagc ggctctgatg ccgcctccct gagctccctc accacctccg cctccgacca
5941 ggatcaggac tacaactacc ttaacgagtg gggaagtcga ttcaagaaac tggcggacat
6001 gtatggtggc ggtgaggatg actaggctgt ccctgccaag ctgtccaggc aagaactaca
6061 gacgggtcac agcagcatct caagggagcc tggctcccgc tccagccagg actctgaagt
6121 ttgcccagaa gtggcctgcg tggcacgctg catctggacc agaaggctgg actgtgagcc
6181 tttccgatgg aaggatgtga ctctgacgac aacatgatcc ccacctcagc catggctgag
6241 cccctctcag agcgggaatg tctaaaggtc tcccgcctgc cacacaacac tcagctctgc
6301 cctctcaggg cttgaacttg ctccgatgcc ctcggtgact ttccccactt ggaatggctc
6361 cctctcttta gaaagaggcc cctaactaag gccctgtttt tctttaatgc agttttcaaa
6421 aagaggctgg ggtgggtggg gcagggtgcg gacatcagtg tcccctcagg ccctccagaa
6481 gagaagccta cagggcactg gccctggtac atttctctga catttcttga ggcctcacat
6541 tggggagggg tcactgcctt tttttttttt ttaatactga gtatatctaa gttgtctcgt
6601 atttttttat tttccctgtt atgtgctgta gatggagagt gatgacaatc gtgtaaatgt
6661 actagaattt ttttattaaa atgaactttt ttttttcccc cgaattcctg cagcccaact
6721 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
6781 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
6841 atgtctggat ccccgggtac cgagctcgaa ttcgtaatca tggtcatagc tgtttcctgt
6901 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa
6961 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc
7021 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag
7081 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
7141 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
7201 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
7261 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
7321 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
7381 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
7441 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
7501 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
7561 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
7621 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
7681 tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat
7741 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
7801 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
7861 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
7921 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
7981 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
8041 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
8101 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
8161 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
8221 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
8281 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
8341 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
8401 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
8461 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
8521 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
8581 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
8641 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
8701 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
8761 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
8821 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
8881 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
8941 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
9001 ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat
9061 gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtc
//