LOCUS LT727539 9058 bp DNA circular SYN 06-FEB-2017
DEFINITION Bacterial expression vector pPLa2311trpA1, complete sequence.
ACCESSION LT727539
VERSION LT727539.1
KEYWORDS .
SOURCE Bacterial expression vector pPLa2311trpA1
ORGANISM Bacterial expression vector pPLa2311trpA1
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 9058)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..9058
/organism="Bacterial expression vector pPLa2311trpA1"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 271."
/db_xref="taxon:1944687"
regulatory complement(117..247)
/note="PL"
/regulatory_class="promoter"
CDS 615..1430
/transl_table=11
/note="kanamycin resistance"
/protein_id="SJL88788.1"
/translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLY
GKPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIP
GKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGL
VDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVG
RVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
rep_origin complement(1716..2107)
/note="pMB1 ori"
CDS complement(2648..3508)
/transl_table=11
/note="ampicillin resistance"
/protein_id="SJL88789.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
CDS complement(7092..7898)
/transl_table=11
/note="trpA"
/protein_id="SJL88790.1"
/translation="MERYENLFAQLNDRREGAFVPFVTLGDPGIEQSLKIIDTLIDAG
ADALELGVPFSDPLADGPTIQNANLRAFAAGVTPAQCFEMLALIREKHPTIPIGLLMY
ANLVFNNGIDAFYARCEQVGVDSVLVADVPVEESAPFRQAALRHNIAPIFICPPNADD
DLLRQVASYGRGYTYLLSRSGVTGAENRGALPLHHLIEKLKEYHAAPALQGFGISSPE
QVSAAVRAGAAGAISGSAIVKIIEKNLASPKQMLAELRSFVSAMKAASRA"
BASE COUNT 2176 a 2216 c 2238 g 2428 t
ORIGIN
1 ttccaatgct tcgtttcgta tcacacaccc caaagccttc tgctttgaat gctgcccttc
61 ttcagggctt aattttaaga gcgtcacctt catggtggtc agtgcgtcct gctgatgtgc
121 tcagtatcac cgccagtggt atttatgtca acaccgccag agataattta tcaccgcaga
181 tggttatctg tatgtttttt atatgaattt attttttgca ggggggcatt gtttggtagg
241 tgagagatcc tctacgccgg acgcatcgtg gccggcatca ccggcgctga ggtctgcctc
301 gtgaagaagg tgttgctgac tcataccagg cctgaatcgc cccatcatcc agccagaaag
361 tgagggagcc acggttgatg agagctttgt tgtaggtgga ccagttggtg attttgaact
421 tttgctttgc cacggaacgg tctgcgttgt cgggaagatg cgtgatctga tccttcaact
481 cagcaaaagt tcgatttatt caacaaagcc acgttgtgtc tcaaaatctc tgatgttaca
541 ttgcacaaga taaaaatata tcatcatgaa caataaaact gtctgcttac ataaacagta
601 atacaagggg tgttatgagc catattcaac gggaaacgtc ttgctcgagg ccgcgattaa
661 attccaacat ggatgctgat ttatatgggt ataaatgggc tcgcgataat gtcgggcaat
721 caggtgcgac aatctatcga ttgtatggga agcccgatgc gccagagttg tttctgaaac
781 atggcaaagg tagcgttgcc aatgatgtta cagatgagat ggtcagacta aactggctga
841 cggaatttat gcctcttccg accatcaagc attttatccg tactcctgat gatgcatggt
901 tactcaccac tgcgatcccc gggaaaacag cattccaggt attagaagaa tatcctgatt
961 caggtgaaaa tattgttgat gcgctggcag tgttcctgcg ccggttgcat tcgattcctg
1021 tttgtaattg tccttttaac agcgatcgcg tatttcgtct cgctcaggcg caatcacgaa
1081 tgaataacgg tttggttgat gcgagtgatt ttgatgacga gcgtaatggc tggcctgttg
1141 aacaagtctg gaaagaaatg cataagcttt tgccattctc accggattca gtcgtcactc
1201 atggtgattt ctcacttgat aaccttattt ttgacgaggg gaaattaata ggttgtattg
1261 atgttggacg agtcggaatc gcagaccgat accaggatct tgccatccta tggaactgcc
1321 tcggtgagtt ttctccttca ttacagaaac ggctttttca aaaatatggt attgataatc
1381 ctgatatgaa taaattgcag tttcatttga tgctcgatga gtttttctaa tcagaattgg
1441 ttaattggtt gtaacactgg cagagcatta cgctgacttg acgggacggc ggctttgttg
1501 aataaatcga acttttgctg agttgaagga tcagatcacg catcttcccg acaacgcaga
1561 ccgttccgtg gcaaagcaaa agttcaaaat caccaactgg tccacctaca acaaagctct
1621 catcaaccgt ggctccctca ctttctggct ggatgatggg gcgattcagg cctggtatga
1681 gtcagcaaca ccttcttcac gaggcagacc tcagcgcttt tccgcttcct cgctcactga
1741 ctcgctacgc tcggtcgttc gactgcggcg agcggtactg actcacacaa aaacggtaac
1801 acagttatcc acagaatcag gggataaggc cggaaagaac atgtgagcaa aagaccagga
1861 acaggaagaa ggccacgtag caggcgtttt tccataggct ccgcccccct gacgagcatc
1921 acaaaaatag acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
1981 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
2041 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
2101 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
2161 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
2221 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
2281 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg
2341 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
2401 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
2461 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
2521 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
2581 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
2641 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
2701 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
2761 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
2821 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
2881 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
2941 tgcgcaacgt tgttgccatt gctgcaggca tcgtggtgtc acgctcgtcg tttggtatgg
3001 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
3061 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
3121 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
3181 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
3241 cgagttgctc ttgcccggcg tcaacacggg ataataccgc gccacatagc agaactttaa
3301 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
3361 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
3421 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
3481 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
3541 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
3601 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta
3661 tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtctt caagaattct
3721 ttatgcgtgc aggtccggca accgtcagac ctacagaagg ggcgggtggt acgctggggc
3781 atttaaacgg gtttgatgtg agtaataaca cgcaactcgg cctgacattc acttacatgg
3841 cgacggacaa tatcggggtt gaattgctgg cggctacgcc gttccgccat aaggtgggta
3901 cgggcgcgac gggcgatatc gccacggtcc acctgttacc gccaacgtta atggcgcagt
3961 ggtacttcgg cgattccagc agcaaagtgc gtccttatgt cggcgtgggg gtgaactaca
4021 ccaccttctt cgataacgac tttaacgata atggtaaaaa tgccgggctg tccgatctga
4081 gctttaagga ttcctggggc gcggcagggc aagttggggt agattacctg attaaccgcg
4141 actggttaat tggcgcctct gtctggtaca tggatattga tactaccgcc aattataaaa
4201 tgggcggcgt tcagcatcat gacagcgtgc gtctggaccc atgggtattt atgttctcgg
4261 caggctatcg tttctaatcg cgtgatgttg tgacgaaaaa agaccccgcc aggcggggtc
4321 aaaaaaggac atgacacacg gattttactt gtcggaactc acttgtcttc tttcggttcg
4381 ccgcagccta gttctgcgcc agtgagagga tcaacggtgg ggtccgattt cgttctttct
4441 gccatcgctt tgagcagcgc catctgctcg cgcggcaatt tcactgtcgc caggccatcg
4501 ccgccatcaa cagcaggcat cggttctgcg acatagttga agttctcatc gctattccag
4561 ctacctcgcg gatcgtcgcc ttgcgacatg ttgtagtaca cgtcggtata ctgttctacc
4621 ggcggtaatt tacccggcgg gaagttgtta cgaatcgaat acagcgcctt ttcaaaagag
4681 agctggtgcg cgacttcacg ggtcattaag aaactgagtg tgtcttttac gccaggatcg
4741 tcggtcagat taatcaggcg ttcataaatg atcttcgctc gcgcctctgc cgcgatgttt
4801 gagcgtagat cggctgtgac ttcgccgata gtatcaatat aagccgcggt ccacggtacg
4861 ccgccggagt ttgtcaacgc cgggccgccg ccatacagca gagaggtgat atggctatca
4921 ttaccattct gcgtcagcga gcgataaagt tccgcttcgt tttcggtacc ttccgccagc
4981 tcgcctttag cgcctttatt aagcattcct accagtgagc cgataatttc cagatgactc
5041 agctcctcgg tggcgatgtc cattaacatt tctctacgac cggcatcatc atcgcttaaa
5101 ccttgcgtga agtagcggca ggcggccgcc agttcgccct gcgggccgcc aaactgttcc
5161 agcagcaggt tcgctaatcc aggattaggt tcgctcactc gcacagtata ttgaagttgt
5221 tttacgtgtc gaaacatatt gcctcctgct tattatttat tttttggctt caacgccatc
5281 tgcttcagat cgcagtaaaa attgttccgt tgtctgcgga atatgtttga taagccagtc
5341 agccatttgc atttcttctt tcagaatggc ttcaatcgtc ggaattgagg cagtgtcgcc
5401 agcttttttt gccgccgcca gcagggaggt ataacaagca atttcgaact gctcgaaaac
5461 ataaccgcta attgaacctt tgacaatttc atcggaaggg aacatgccac cgatagattg
5521 ccccattgct gccattttac tcatggagtc tttcaacacc gaacgggaaa tgccattacg
5581 gtccaacact tcttcgagca tggtaatttg atgtttggtt tcactaatat gttgttcaat
5641 tctggctttt atatcaggat aattttcaat acggctggcc atagattcaa gcatcgattc
5701 tgcctgtttt tccatggcat gggcgtcacg aagccagtca tgataatgtt cagtataatt
5761 cattttacat tcctcaatgc gtgataatct gctccgataa taaaatcgga atcatgcaag
5821 ctgataatta aaaatattat ttcgatttgc gttcagcact tttattaaca ttgctgactg
5881 ctaaatcggt aagttttaaa tcagtttgtt tttcctcgtc gagggtttct ttgagcagtt
5941 ttaatgcttt gctatagccg agctgctcgg ccagggtggc tagcgtgccg tagctggcga
6001 tttcgtaatg ctcgactttt tgcgccgcgg cgataagcgc tgcatcgcgt acttcgtttt
6061 tctccgtact ttcgatgact tcattggctt cttcaatcag cccttccata gcgacgcatt
6121 tcattctttt cagtttaatg ccagattcag attcgacgat ctgatcaata cgttcaatct
6181 gaccctgggt ttcttcaaga tgagattgaa aggcctggct taatttttca ttggacgtgg
6241 ctctggcaag tttaggaaga gccttggtta attgtttttc tgcactatag gtatctgaaa
6301 gtagatggat aaaaaggtct tcaacggttt tgatattcat aaatctaatt ctcctgggca
6361 aaatactgcg agccagaaga ctcgcagatt gcttgataaa agcatgtgtt atatttacat
6421 tacagtaaat caggaattat cggatttacg tccgccgtga ctattctgac cacctttttt
6481 accggcttca gaagctcgtt gcggatcgtt tttgaaattc ccgccgctat gctgaccgcc
6541 tttgcggccc gcttcagatg ctttttcacg attctcggcg aaatttcctg aaccaccacg
6601 atgttcggcc atattatttc tcctgtctcg ttgcttgctg aaaaaatcgg ttttaaccgt
6661 gtcgacaccc tattaaaatt agttggttaa accgattagt caaattgacg gaaatagttg
6721 tgatttaccg cgtaaataac gcacaaaaaa tggtttagag ataatatatt ctatattttc
6781 ttttagaata agcgatttaa caggaaatgc tttgcattgt tattaataac aatcgtcact
6841 atctgcggcc ctggaaacgt taatgcattg tttgttgaca tttctttaca taaaggcggg
6901 ggaaattgac gtgaaaacgc taaatttttc ggcgtgatta ggagttttct tagcaaaaat
6961 tgacttcaga ctttttgaaa gctggacggg gaataacacc aaaaagattg atgatgtacg
7021 tctgaatcgt gattctggtt cggcgccagc cactcattaa gccgccagcg ttatgctgac
7081 ggcttaaccc gttatgcgcg gctggcggct ttcatggctg agacaaagga cctgagctcc
7141 gccaacatct gtttgggaga cgcgaggttt ttctcgataa tcttgacaat ggctgagccg
7201 gagatagcgc cagccgcccc ggcacgcacg gccgcagaca cctgttccgg cgaggagata
7261 ccgaagccct gtaacgcagg cgcggcatgg tactctttaa gcttctcaat gagatgatgc
7321 aacggcaatg cgccacggtt ttccgcgccg gtgacacccg aacgcgaaag caggtaggtg
7381 taaccgcggc cgtaagatgc gacctggcgc agaagatcgt catccgcatt tggcgggcag
7441 atgaagatcg gcgcgatatt atgccgtaac gctgcctggc ggaagggggc cgattcttca
7501 accgggacat ctgcgaccag cacggaatct acgccaacct gttcacaacg ggcatagaac
7561 gcatctatgc cgttattgaa caccagattc gcgtacatta gcaggccaat cggaatggtc
7621 gggtgttttt cacgaatcag cgccagcatt tcaaaacact gagccggcgt gacgccagcg
7681 gcgaaggcgc gtaagttcgc attctggatg gtagggccat cggccagcgg atcggagaag
7741 ggaaccccca gttccagagc gtcggcgcca gcgtcaatca gtgtgtcaat aattttcagt
7801 gactgttcaa tgccagggtc gcccagggtc acgaagggga caaaagcgcc ttcccggcga
7861 tcgttgagtt gggcaaataa attttcgtag cgttccatca gatttcccct cgcgctttca
7921 ggatatcgtg tacggtaaag atgtctttat ctccgcggcc agagagattg accaccagca
7981 gttgctcttt ttccggctgc tcgcgcatca ttttcagagc gtgcgccaac gcgtgggagg
8041 actccagcgc cgggataatt ccctcatggc ggcacaacgt tttgaaggct tccagcgcct
8101 catcatcggt aatggagaca taatccgcgc gtccgatgct gttcaggtac gcatgttgcg
8161 gcccaacgga cgggaaatcg agcccggcgg aaatggaata ggactcttca atttgcccgt
8221 ctgctgtttg catcatcggc gctttcatcc cgaaatagat gccaacgcga ccatgtttaa
8281 gcggcgcgcc atgctcgccg gtttcaatac catgaccacc aggttcaacg cctattagcc
8341 cgacgctggt atcattaata aaatcggcaa acatcccgat agcgtttgag ccgccaccga
8401 cgcaagcgat aacggcatct ggcagacggc cctctttgtc gaggatttgc gctttcgtct
8461 cttcgccaat catgcgctgg aactcgcgaa cgatggtggg atagggatgc ggtcctgccg
8521 ccgtgccgag catatagtgc gcggtttcgt aactaccgga ccagtcgcgc agcgcctcgt
8581 tacaggcatc ttttagcgta gcggagccgc tatgaaccgg gatgacctca gcgcccatca
8641 gacgcatacg gaagacgttc ggcgactggc gctcaacgtc tttggcgccc atatagatac
8701 ggcatttcag acccagcagg gcgctggcga gcgcagaggc gacgccgtgc tgaccggcgc
8761 cggtttcagc gataatctcg cttttaccca tccgtttggc cagcagcgcc tgacccagta
8821 cctgattggt tttgtgcgcg ccgccgtgca gtaaatcttc gcgctttaaa tacaacgtgg
8881 tacgcgtacc ggcggtaatg ttctggcatt tcgtcagcgc ggtggggcgt cccgcgtagt
8941 tttttagcag atcggcgaat tgcgcctgaa attcaggatc tttttgcgcg ctgacgaagg
9001 cctcttcaag ctggttcagc gcaggcatca ggatctgcgg cacatacatg ccgccgaa
//