LOCUS LT727536 5258 bp DNA circular SYN 06-FEB-2017
DEFINITION Bacterial expression vector pPLaR1, complete sequence.
ACCESSION LT727536
VERSION LT727536.1
KEYWORDS .
SOURCE Bacterial expression vector pPLaR1
ORGANISM Bacterial expression vector pPLaR1
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 5258)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..5258
/organism="Bacterial expression vector pPLaR1"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 269."
/db_xref="taxon:1944691"
regulatory complement(117..247)
/note="PL"
/regulatory_class="promoter"
CDS 615..1430
/transl_table=11
/note="kanamycin resistance"
/protein_id="SJL88775.1"
/translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLY
GKPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIP
GKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGL
VDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVG
RVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
rep_origin complement(1710..2110)
/note="pMB1 ori"
misc_feature complement(2965..3154)
/note="IS1"
CDS complement(3491..5128)
/transl_table=11
/note="POL"
/protein_id="SJL88776.1"
/translation="MSKTTKKFNSLCIDLPRDLSLEIYQSIASVATGSGDPHSDDFTA
IAYLRDELLTKHPTLGSGNDEATRRTLAIAKLREANGDRGQINREGFLHDKSLSWDPD
VLQTSIRSLIGNLLSGYRSSLFGQCTFSNGAPMGHKLQDAAPYKKFAEQATVTPRALR
AALLVRDQCAPWIRHAVRYNESYEFRLVVGNGVFTVPKNNKIDRAACKEPDMNMYLQK
GVGAFIRRRLKSVGIDLNDQSINQRLAQQGSVDGSLATIDLSSASDSISDRLVWSFLP
PELYSYLDRIRSHYGIVDGETIRWELFSTMGNGFTFELESMIFWAIVKATQIHFGNAG
TIGIYGDDIICPSEIAPRVLEALAYYGFKPNLRKTFVSGLFRESCGAHFYRGVDVKPF
YIKKPVDNLFALMLILNRLRGWGVVGGMSDPRLYKVWVRLSSQVPSMFFGGTDLAADY
YVVSPPTAVSVYTKTPYGRLLADTRTSGFRLARIARERKFFSEKHDSGRYIAWFHTGG
EITDSMKSAGVRVIRTSEWLTPVPTFPQECGPASSPR"
CDS complement(4984..5211)
/transl_table=11
/note="LYS"
/protein_id="SJL88777.1"
/translation="METRFPQQSQQTPASTNRRRPFKHEDYPCRRQQRSSTLYVLIFL
AIFLSKFTNQLLLSLLEAVIRTVTTLQQLLT"
misc_feature complement(5129..5161)
/note="5UTR"
CDS complement(5162..>5258)
/codon_start=2
/transl_table=11
/note="COATf"
/protein_id="SJL88778.1"
/translation="SDCELIVKAMQGLLKDGNPIPSAIAANSGIY"
BASE COUNT 1425 a 1257 c 1272 g 1304 t
ORIGIN
1 ttccaatgct tcgtttcgta tcacacaccc caaagccttc tgctttgaat gctgcccttc
61 ttcagggctt aattttaaga gcgtcacctt catggtggtc agtgcgtcct gctgatgtgc
121 tcagtatcac cgccagtggt atttatgtca acaccgccag agataattta tcaccgcaga
181 tggttatctg tatgtttttt atatgaattt attttttgca ggggggcatt gtttggtagg
241 tgagagatcc tctacgccgg acgcatcgtg gccggcatca ccggcgctga ggtctgcctc
301 gtgaagaagg tgttgctgac tcataccagg cctgaatcgc cccatcatcc agccagaaag
361 tgagggagcc acggttgatg agagctttgt tgtaggtgga ccagttggtg attttgaact
421 tttgctttgc cacggaacgg tctgcgttgt cgggaagatg cgtgatctga tccttcaact
481 cagcaaaagt tcgatttatt caacaaagcc acgttgtgtc tcaaaatctc tgatgttaca
541 ttgcacaaga taaaaatata tcatcatgaa caataaaact gtctgcttac ataaacagta
601 atacaagggg tgttatgagc catattcaac gggaaacgtc ttgctcgagg ccgcgattaa
661 attccaacat ggatgctgat ttatatgggt ataaatgggc tcgcgataat gtcgggcaat
721 caggtgcgac aatctatcga ttgtatggga agcccgatgc gccagagttg tttctgaaac
781 atggcaaagg tagcgttgcc aatgatgtta cagatgagat ggtcagacta aactggctga
841 cggaatttat gcctcttccg accatcaagc attttatccg tactcctgat gatgcatggt
901 tactcaccac tgcgatcccc gggaaaacag cattccaggt attagaagaa tatcctgatt
961 caggtgaaaa tattgttgat gcgctggcag tgttcctgcg ccggttgcat tcgattcctg
1021 tttgtaattg tccttttaac agcgatcgcg tatttcgtct cgctcaggcg caatcacgaa
1081 tgaataacgg tttggttgat gcgagtgatt ttgatgacga gcgtaatggc tggcctgttg
1141 aacaagtctg gaaagaaatg cataagcttt tgccattctc accggattca gtcgtcactc
1201 atggtgattt ctcacttgat aaccttattt ttgacgaggg gaaattaata ggttgtattg
1261 atgttggacg agtcggaatc gcagaccgat accaggatct tgccatccta tggaactgcc
1321 tcggtgagtt ttctccttca ttacagaaac ggctttttca aaaatatggt attgataatc
1381 ctgatatgaa taaattgcag tttcatttga tgctcgatga gtttttctaa tcagaattgg
1441 ttaattggtt gtaacactgg cagagcatta cgctgacttg acgggacggc ggctttgttg
1501 aataaatcga acttttgctg agttgaagga tcagatcacg catcttcccg acaacgcaga
1561 ccgttccgtg gcaaagcaaa agttcaaaat caccaactgg tccacctaca acaaagctct
1621 catcaaccgt ggctccctca ctttctggct ggatgatggg gcgattcagg cctggtatga
1681 gtcagcaaca ccttcttcac gaggcagacc tcagcgcttt tccgcttcct cgctcactga
1741 ctcgctacgc tcggtcgttc gactgcggcg agcggtactg actcacacaa aaacggtaac
1801 acagttatcc acagaatcag gggataaggc cggaaagaac atgtgagcaa aagaccagga
1861 acaggaagaa ggccacgtag caggcgtttt tccataggct ccgcccccct gacgagcatc
1921 acaaaaatag acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
1981 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
2041 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
2101 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
2161 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
2221 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
2281 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg
2341 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
2401 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
2461 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
2521 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
2581 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
2641 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
2701 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
2761 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
2821 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
2881 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
2941 tgcgcaacgt tgttgccatt gctgcagttg ccatgtttta cggcagtgag agcagagata
3001 gcgctgatgt ccggcggtgc ttttgccgtt acgcaccacc ccgtcagtag ctgaacagga
3061 gggacagctg atagaaacag aagccactgg agcacctcaa aaacaccatc atacactaaa
3121 tcagtaagtt ggcagcatca cccacgatgc ctgcaaaaaa aaaaaaaaaa aaaaaaaaaa
3181 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
3241 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
3301 aaaaattttt tttttttttt gggtggtaac tagccaagca gctagttacc aaatcgggag
3361 aatcccgggt cctctcttta gggggaggtc cctgggccga agcccgccca cctttcggtg
3421 gagccggacc gctttcgcac ccgtgctctt tcgagcacac ccaccccgtt tacgggggtc
3481 cctcggtcag ctaccgagga gagctcgctg gcccacactc ctgagggaat gtgggaaccg
3541 gcgttagcca ctccgaagtg cgtataacgc gcacgccggc ggacttcatg ctgtcggtga
3601 tttcacctcc agtatggaac cacgctatgt agcgaccact gtcgtgcttt tcgctgaaga
3661 acttgcgttc tcgagcgata cgagcaagac ggaaacccga ggtacgggta tccgcgagca
3721 gccgcccgta cggagtcttg gtgtataccg agactgccgt aggcgggctg actacgtagt
3781 agtcggcagc gaggtccgtc ccaccgaaga acatcgaagg cacctgggag gagagccgta
3841 cccacacctt atagaggcgt ggatctgaca tacctccgac aactccccaa ccccgtagcc
3901 gatttaatat cagcatcagg gcgaagagat tgtcaacagg tttcttgatg taaaacggtt
3961 tgacatcgac accacggtaa aagtgcgcgc cgcagctctc gcgaaagagc ccggacacga
4021 acgttttacg aagattcggt ttaaaaccgt agtaggcaag tgcctctagc acacggggtg
4081 caatctcact gggacatata atatcgtccc cgtagatgcc tatggttccg gcgttaccaa
4141 aatggatttg ggtcgctttg actattgccc agaatatcat ggactctagc tcaaatgtga
4201 acccatttcc cattgtggaa aatagttccc atcgtatcgt ctcgccatct acgattccgt
4261 agtgtgagcg gatacgatcg agatatgaat atagctcagg tgggagaaaa ctccacacca
4321 ggcgatcgga gatggaatcg gatgcagacg ataagtctat cgtcgcaagc gaaccatcta
4381 cgctgccctg ctgagccaga cgctggttga tcgattgatc attcaggtct ataccaacgg
4441 atttgagccg gcgtctgatg aaagcaccga cccctttctg gaggtacata ttcatatcag
4501 gctccttaca ggcagcccga tctattttat tattcttcgg aactgtaaac actccgttcc
4561 ctacaacgag cctaaattca tatgactcgt tatagcggac cgcgtgtctg atccacggcg
4621 cacattggtc tcggaccaat agagccgctc tcagagcgcg gggggtaacg gttgcttgtt
4681 cagcgaactt cttgtaaggc gctgcatcct gcaacttgtg ccccatagga gcaccgttgg
4741 agaacgtgca ttgcccaaac aacgacgatc ggtagccaga gaggaggttg ccaataaggc
4801 tacggatgct ggtttgtaaa acatccggat cccatgacaa ggatttgtca tgtaagaaac
4861 cttctctatt tatctgaccg cgatcaccat tcgcctcccg tagcttagcg atagctaagg
4921 tacgacgggt cgcctcgtca ttaccagaac ctaaggtcgg atgctttgtg agcaattcgt
4981 cccttaagta agcaattgct gtaaagtcgt cactgtgcgg atcaccgctt ccagtagcga
5041 cagaagcaat tgattggtaa atttcgagag aaagatcgcg aggaagatca atacataaag
5101 agttgaactt ctttgttgtc ttcgacatgg gtaatcctca tgtttgaatg gccggcgtct
5161 attagtagat gccggagttt gctgcgattg ctgagggaat cgggtttcca tcttttagga
5221 gaccttgcat tgccttaaca ataagctcgc agtcggaa
//