LOCUS LT727534 6207 bp DNA circular SYN 06-FEB-2017
DEFINITION Vector pATHIL6HIFNG, complete sequence.
ACCESSION LT727534
VERSION LT727534.1
KEYWORDS .
SOURCE Vector pATHIL6HIFNG
ORGANISM Vector pATHIL6HIFNG
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 6207)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..6207
/organism="Vector pATHIL6HIFNG"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 2672."
/db_xref="taxon:1945244"
misc_feature 19..640
/note="5FR"
regulatory 641..1175
/note="hIL6 promoter"
/regulatory_class="promoter"
misc_difference 1249..1317
/note="hIFNG signal seq."
CDS <1318..>1746
/codon_start=1
/transl_table=11
/note="hIFNG"
/protein_id="SJL88770.1"
/translation="QDPYVKEAENLKKYFNAGHSDVADNGTLFLGILKNWKEESDRKI
MQSQIVSFYFKLFKNFKDDQSIQKSVETIKEDMNVKFFNSNKKKRDDFEKLTNYSVTD
LNVQRKAIHELIQVMAELSPAAKTGKRKRSQMLFRGRRASQ"
misc_feature 2078..2149
/note="part of SV40 small t-antigen"
misc_feature 2153..2219
/note="intron"
misc_feature 2220..2771
/note="3UTR"
regulatory 2772..2868
/note="SV40 polyA"
/regulatory_class="terminator"
regulatory complement(2772..2868)
/note="SV40 polyA"
/regulatory_class="terminator"
rep_origin complement(4196..4600)
/note="pMB1 ori"
CDS complement(<5142..5999)
/transl_table=11
/note="ampicillin resistance"
/protein_id="SJL88771.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
BASE COUNT 1653 a 1482 c 1457 g 1615 t
ORIGIN
1 ttcgagctcg cccggggatc ctcctgcaag agacaccatc ctgagggaag agggcttctg
61 aaccagcttg acccaataag aaattcttgg gtgccgacgc ggacagagat tcagagccta
121 gagccgtgcc tgcgtccgta ctttccttct agcttctttt gatttcaaat caagacttag
181 agggagaggg agcgataaac acaaactctg caagatgcca caaggtcctc ctttgacatc
241 cccaacaaag aggtgagtag taatctcccc ctttctgccc tgaaccaagt gggcttcagt
301 aatttcaggg ctccaggaga ctgggcatgc aggtgccgat gaaacagtgg tgaagagact
361 cagtggcagt ggggagagca ctggcagcac aggcaaacct ctggcacaag agcaaagtcc
421 tactggagat tccaagggtc acttgggaga gggcaggcag cagccaacct cctctaagtg
481 ggctgaagca ggtgaagaaa tggcagacaa gcgcggtggc aaaaaggagt cacacactcc
541 acctggagac gccttgaagt aactgcacga aatttgaggg tggccaggca gtctacaaca
601 gccgctcaca gggagagcca gaacacagaa gaactcagat gactggtagt attaccttct
661 tcataatcca ggcttggggg gctgcgatgg agtcagagga aactcagttc agaacatctt
721 tggtttttac aaatacaaat taactggaac gctaaattct agcctgttaa tctggtcact
781 gaaaaaaaaa tttttttttt tcaaaaaaca tagctttagc ttattttttt tctctttgta
841 aaacttcgtg catgacttca gctttactct ttgtcaagac atgccaaagt gctgagtcac
901 taataaaaga aaaaaagaaa gtaaaggaag agtggttctg cttcttagcg ctagcctcaa
961 tgacgaccta agctgcactt ttccccctag ttgtgtcttg cgatgctaaa ggacgtcaca
1021 ttgcacaatc ttaataaggt ttccaatcag ccccacccgc tctggcccca ccctcaccct
1081 ccaacaaaga tttatcaaat gtgggatttt cccatgagtc tcaatattag agtctcaacc
1141 cccaataaat ataggactgg agatgtctga ggctcattct gccctcgagg tcagcttgat
1201 acaagaacta ctgatttcaa cttctttggc ttaattctct cggaaacgat gaaatataca
1261 agttatatct tggcttttca gctctgcatc gttttgggtt ctcttggctg ttactgccag
1321 gacccatatg taaaagaagc agaaaacctt aagaaatatt ttaatgcagg tcattcagat
1381 gtagcggata atggaactct tttcttaggc attttgaaga attggaaaga ggagagtgac
1441 agaaaaataa tgcagagcca aattgtctcc ttttacttca aactttttaa aaactttaaa
1501 gatgaccaga gcatccaaaa gagtgtggag accatcaagg aagacatgaa tgtcaagttt
1561 ttcaatagca acaaaaagaa acgagatgac ttcgaaaagc tgactaatta ttcggtaact
1621 gacttgaatg tccaacgcaa agcaatacat gaactcatcc aagtgatggc tgaactgtcg
1681 ccagcagcta aaacagggaa gcgaaaaagg agtcagatgc tgtttcgagg tcgaagagca
1741 tcccagtaat ggttgtcctg cctgcaatat ttgaatttta aatctaaatc tatttattaa
1801 tatttaacat tatttatatg gggaatatat ttttagactc atcaatcaaa taagtattta
1861 taatagcaac ttttgtgtaa tgaaaatgaa tatctattaa tatatgtatt atttataatt
1921 cctatatcct gtgactgtct cacttaatcc tttgttttct gactaattag gcaaggctat
1981 gtgattacaa ggctttatct caggggccaa ctaggcagcc aacctaagca agacctaggc
2041 ttttgcaaaa agctcaagct tggctctaga gtcgacggat ctttgtgaag gaaccttact
2101 tctgtggtgt gacataattg gacaaactac ctacagagat ttaaagctct aaggtaaata
2161 taaaattttt aagtgtataa tgtgttaaac tactgattct aattgtttgt gtattttaga
2221 ttccaaccta tggaactgat gaatgggagc agtggtggaa tgcctttaat gaggaaaacc
2281 tgttttgctc agaagaaatg ccatctagtg atgatgaggc tactgctgac tctcaacatt
2341 ctactcctcc aaaaaagaag agaaaggtag aagaccccaa ggactttcct tcagaattgc
2401 taagtttttt gagtcatgct gtgtttagta atagaactct tgcttgcttt gctatttaca
2461 ccacaaagga aaaagctgca ctgctataca agaaaattat ggaaaaatat tctgtaacct
2521 ttataagtag gcataacagt tataatcata acatactgtt ttttcttact ccacacaggc
2581 atagagtgtc tgctattaat aactatgctc aaaaattgtg tacctttagc tttttaattt
2641 gtaaaggggt taataaggaa tatttgatgt atagtgcctt gactagagat cataatcagc
2701 cataccacat ttgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac
2761 ctgaaacata aaatgaatgc aattgttgtt gttaacttgt ttattgcagc ttataatggt
2821 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
2881 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggatcct ctacgccgga
2941 cgcatcgtgg ccggcatcac cggcgccaca ggtgcggttg ctggcgccta tatcgccgac
3001 atcaccgatg gggaagatcg ggctcgccac ttcgggctca tgagcgcttg tttcggcgtg
3061 ggtatggtgg caggccccgt ggccggggga ctgttgggcg ccatctcctt gcatgcacca
3121 ttccttgcgg cggcggtgct caacggcctc aacctactac tgggctgctt cctaatgcag
3181 gagtcgcata agggagagcg tcgaccgatg cccttgagag ccttcaaccc agtcagctcc
3241 ttccggtggg cgcggggcat gactatcgtc gccgcactta tgactgtctt ctttatcatg
3301 caactcgtag gacaggtgcc ggcagcgctc tgggtcattt tcggcgagga ccgctttcgc
3361 tggagcgcga cgatgatcgg cctgtcgctt gcggtattcg gaatcttgca cgccctcgct
3421 caagccttcg tcactggtcc cgccaccaaa cgtttcggcg agaagcaggc cattatcgcc
3481 ggcatggcgg ccgacgcgct gggctacgtc ttgctggcgt tcgcgacgcg aggctggatg
3541 gccttcccca ttatgattct tctcgcttcc ggcggcatcg ggatgcccgc gttgcaggcc
3601 atgctgtcca ggcaggtaga tgacgaccat cagggacagc ttcaaggatc gctcgcggct
3661 cttaccagcc taacttcgat cactggaccg ctgatcgtca cggcgattta tgccgcctcg
3721 gcgagcacat ggaacgggtt ggcatggatt gtaggcgccg ccctatacct tgtctgcctc
3781 cccgcgttgc gtcgcggtgc atggagccgg gccacctcga cctgaatgga agccggcggc
3841 acctcgctaa cggattcacc actccaagaa ttggagccaa tcaattcttg cggagaactg
3901 tgaatgcgca aaccaaccct tggcagaaca tatccatcgc gtccgccatc tccagcagcc
3961 gcacgcggcg catctcgggc agcgttgggt cctggccacg ggtgcgcatg atcgtgctcc
4021 tgtcgttgag gacccggcta ggctggcggg gttgccttac tggttagcag aatgaatcac
4081 cgatacgcga gcgaacgtga agcgactgct gctgcaaaac gtctgcgacc tgagcaacaa
4141 catgaatggt cttcggtttc cgtgtttcgt aaagtctgga aacgcggaag tcagcgctct
4201 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
4261 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
4321 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
4381 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
4441 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
4501 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
4561 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
4621 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
4681 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
4741 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
4801 aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc
4861 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
4921 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
4981 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
5041 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
5101 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
5161 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
5221 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
5281 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
5341 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
5401 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctgcaggc
5461 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
5521 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
5581 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
5641 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
5701 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaacacgg
5761 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
5821 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
5881 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
5941 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
6001 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
6061 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
6121 gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt
6181 atcacgaggc cctttcgtct tcaagaa
//