LOCUS LT727532 5382 bp DNA circular SYN 06-FEB-2017
DEFINITION Vector pATHIL6pA, complete sequence.
ACCESSION LT727532
VERSION LT727532.1
KEYWORDS .
SOURCE Vector pATHIL6pA
ORGANISM Vector pATHIL6pA
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 5382)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..5382
/organism="Vector pATHIL6pA"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 2659."
/db_xref="taxon:1945245"
misc_feature 23..659
/note="5FR"
regulatory 660..1195
/note="hIL6"
/regulatory_class="promoter"
CDS <1255..1329
/codon_start=1
/transl_table=11
/note="part of SV40 small t-antigen"
/protein_id="SJL88764.1"
/translation="DLCEGTLLLWCDIIGQTTYRDLKL"
misc_feature 1330..1396
/note="intron of SV40 small t-antigen"
misc_feature 1397..1948
/note="3' UTR of SV40 small t-antigen"
regulatory 1949..2045
/note="polyA"
/regulatory_class="terminator"
regulatory complement(1949..2045)
/note="polyA"
/regulatory_class="terminator"
rep_origin complement(3373..3775)
/note="pMB1 ori"
CDS complement(4316..5176)
/transl_table=11
/note="ampicillin resistance"
/protein_id="SJL88765.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
BASE COUNT 1368 a 1349 c 1306 g 1359 t
ORIGIN
1 aattcgagct cgcccgggga tcctcctgca agagacacca tcctgaggga agagggcttc
61 tgaaccagct tgacccaata agaaattctt gggtgccgac gcggaagcag attcagagcc
121 tagagccgtg cctgcgtccg tagtttcctt ctagcttctt ttgatttcaa atcaagactt
181 acagggagag ggagcgataa acacaaactc tgcaagatgc cacaaggtcc tcctttgaca
241 tccccaacaa agaggtgagt agtattctcc ccctttctgc cctgaaccaa gtgggcttca
301 gtaatttcag ggctccagga gacctggggc ccatgcaggt gccccagtga aacagtggtg
361 aagagactca gtggcaatgg ggagagcact ggcagcacaa ggcaaacctc tggcacagag
421 agcaaagtcc tcactgggag gattcccaag gggtcacttg ggagagggca gggcagcagc
481 caacctcctc taagtgggct gaagcaggtg aagaaagtgg cagaagccac gcggtggcaa
541 aaaggagtca cacactccac ctggagacgc cttgaagtaa ctgcacgaaa tttgaggatg
601 gccaggcagt tctacaacag ccgctcacag ggagagccag aacacagaag aactcagatg
661 actggtagta ttaccttctt cataatccca ggcttggggg gctgcgatgg agtcagagga
721 aactcagttc agaacatctt tggtttttac aaatacaaat taactggaac gctaaattct
781 agcctgttaa tctggtcact gaaaaaaaat tttttttttt tcaaaaaaca tagctttagc
841 ttattttttt tctctttgta aaacttcgtg catgacttca gctttactct ttgtcaagac
901 atgccaaagt gctgagtcac taataaaaga aaaaaagaaa gtaaaggaag agtggttctg
961 cttcttagcg ctagcctcaa tgacgaccta agctgcactt ttccccctag ttgtgtcttg
1021 cgatgctaaa ggacgtcaca ttgcacaatc ttaataaggt ttccaatcag ccccacccgc
1081 tctggcccca ccctcaccct ccaacaaaga tttatcaaat gtgggatttt cccatgagtc
1141 tcaatattag agtctcaacc cccaataaat ataggactgg agatgtctga ggctcattct
1201 gccctcgagg cctaggcttt tgcaaaaagc tcaagcttgg ctctagagtc gacggatctt
1261 tgtgaaggaa ccttacttct gtggtgtgac ataattggac aaactaccta cagagattta
1321 aagctctaag gtaaatataa aatttttaag tgtataatgt gttaaactac tgattctaat
1381 tgtttgtgta ttttagattc caacctatgg aactgatgaa tgggagcagt ggtggaatgc
1441 ctttaatgag gaaaacctgt tttgctcaga agaaatgcca tctagtgatg atgaggctac
1501 tgctgactct caacattcta ctcctccaaa aaagaagaga aaggtagaag accccaagga
1561 ctttccttca gaattgctaa gttttttgag tcatgctgtg tttagtaata gaactcttgc
1621 ttgctttgct atttacacca caaaggaaaa agctgcactg ctatacaaga aaattatgga
1681 aaaatattct gtaaccttta taagtaggca taacagttat aatcataaca tactgttttt
1741 tcttactcca cacaggcata gagtgtctgc tattaataac tatgctcaaa aattgtgtac
1801 ctttagcttt ttaatttgta aaggggttaa taaggaatat ttgatgtata gtgccttgac
1861 tagagatcat aatcagccat accacatttg tagaggtttt acttgcttta aaaaacctcc
1921 cacacctccc cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt aacttgttta
1981 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
2041 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct
2101 ggatcctcta cgccggacgc atcgtggccg gcatcaccgg cgccacaggt gcggttgctg
2161 gcgcctatat cgccgacatc accgatgggg aagatcgggc tcgccacttc gggctcatga
2221 gcgcttgttt cggcgtgggt atggtggcag gccccgtggc cgggggactg ttgggcgcca
2281 tctccttgca tgcaccattc cttgcggcgg cggtgctcaa cggcctcaac ctactactgg
2341 gctgcttcct aatgcaggag tcgcataagg gagagcgtcg accgatgccc ttgagagcct
2401 tcaacccagt cagctccttc cggtgggcgc ggggcatgac tatcgtcgcc gcacttatga
2461 ctgtcttctt tatcatgcaa ctcgtaggac aggtgccggc agcgctctgg gtcattttcg
2521 gcgaggaccg ctttcgctgg agcgcgacga tgatcggcct gtcgcttgcg gtattcggaa
2581 tcttgcacgc cctcgctcaa gccttcgtca ctggtcccgc caccaaacgt ttcggcgaga
2641 agcaggccat tatcgccggc atggcggccg acgcgctggg ctacgtcttg ctggcgttcg
2701 cgacgcgagg ctggatggcc ttccccatta tgattcttct cgcttccggc ggcatcggga
2761 tgcccgcgtt gcaggccatg ctgtccaggc aggtagatga cgaccatcag ggacagcttc
2821 aaggatcgct cgcggctctt accagcctaa cttcgatcac tggaccgctg atcgtcacgg
2881 cgatttatgc cgcctcggcg agcacatgga acgggttggc atggattgta ggcgccgccc
2941 tataccttgt ctgcctcccc gcgttgcgtc gcggtgcatg gagccgggcc acctcgacct
3001 gaatggaagc cggcggcacc tcgctaacgg attcaccact ccaagaattg gagccaatca
3061 attcttgcgg agaactgtga atgcgcaaac caacccttgg cagaacatat ccatcgcgtc
3121 cgccatctcc agcagccgca cgcggcgcat ctcgggcagc gttgggtcct ggccacgggt
3181 gcgcatgatc gtgctcctgt cgttgaggac ccggctaggc tggcggggtt gccttactgg
3241 ttagcagaat gaatcaccga tacgcgagcg aacgtgaagc gactgctgct gcaaaacgtc
3301 tgcgacctga gcaacaacat gaatggtctt cggtttccgt gtttcgtaaa gtctggaaac
3361 gcggaagtca gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
3421 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
3481 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
3541 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
3601 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
3661 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
3721 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
3781 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
3841 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
3901 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
3961 tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc
4021 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
4081 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
4141 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
4201 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
4261 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
4321 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
4381 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
4441 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
4501 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
4561 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
4621 ttgccattgc tgcaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
4681 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
4741 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
4801 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
4861 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
4921 gcccggcgtc aacacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
4981 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
5041 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
5101 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
5161 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
5221 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
5281 gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa
5341 cctataaaaa taggcgtatc acgaggccct ttcgtcttca ag
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