LOCUS LT727529 5095 bp DNA circular SYN 06-FEB-2017
DEFINITION Bacterial expression vector pUChTNFR75f, complete sequence.
ACCESSION LT727529
VERSION LT727529.1
KEYWORDS .
SOURCE Bacterial expression vector pUChTNFR75f
ORGANISM Bacterial expression vector pUChTNFR75f
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 5095)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..5095
/organism="Bacterial expression vector pUChTNFR75f"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 2560."
/db_xref="taxon:1944713"
misc_feature complement(403..1512)
/note="3UTR"
CDS complement(1513..>2803)
/codon_start=2
/transl_table=11
/note="TNFRf"
/protein_id="SJL88754.1"
/translation="APEPGSTCRLREYYDQTAQMCCSKCSPGQHAKVFCTKTSDTVCD
SCEDSTYTQLWNWVPECLSCGSRCSSDQVETQACTREQNRICTCRPGWYCALSKQEGC
RLCAPLRKCRPGFGVARPGTETSDVVCKPCAPGTFSNTTSSTDICRPHQICNVVAIPG
NASMDAVCTSTSPTRSMAPGAVHLPQPVSTRSQHTQPTPEPSTAPSTSFLLPMGPSPP
AEGSTGDFALPVGLIVGVTALGLLIIGVVNCVIMTQVKKKPLCLQREAKVPHLPADKA
RGTQGPEQQHLLITAPSSSSSSLESSASALDRRAPTRNQPQAPGVEASGAGEARASTG
SSDSSPGGHGTQVNVTCIVNVCSSSDHSSQCSSQASSTMGDTDSSPSESPKDEQVPFS
KEECAFRSQLETPETLLGSTEEKPLPLGVPDAGMKPS"
regulatory complement(2917..3000)
/note="lac"
/regulatory_class="promoter"
rep_origin complement(3092..3494)
/note="pMB1 ori"
CDS complement(4035..4895)
/transl_table=11
/note="ampicillin resistance"
/protein_id="SJL88755.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
BASE COUNT 1114 a 1379 c 1421 g 1179 t
ORIGIN
1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cnggatgtgg ctggcagagt
421 ttggctttgt cgttggcttg ttggcctgag tggtacttac ttttttaaaa aattggcaga
481 tttctagtta gaagtgcgtt atcttgccca ggccacagca ctgaatatgg tggcctgcct
541 gggccctctg ggcatctgac tttcacacca tggtacaaaa ggacaagtta gcatttggag
601 gcggtgcttt ttttttttct tttgagacag actctcgctc tgtcgcccag gctggagtgc
661 agtggcgtga tctcggctcc ctgcaacctc cgcttcccgg gttcaaacga tttcccagcc
721 tcaggcttct gagtagctgg gactataggt gcccgccacc acgcccggct aatttctgta
781 tttttagtag agatggggtt ttaccatgtt ggccaggctg gtctcgaact cctaacctca
841 ggtgatccac ccgcctcagc ctcccaaagt gctgggatca taggcgtgag ccactgcacc
901 tggcctgagg tgatgctttc caagcctcct gagctaactt gaaggacccc gttccctaca
961 gggctgccac ctcctccctg cacagagccc caggaccatc ccgcagtctc agcctcaggc
1021 tgaagcactg tcctgtcttc atgggtgact caggcagcat ccctcctctc cagaaagccc
1081 ctctgcagaa aaggatgctg gggttttctg gaagccagag ctggggcaga gcccaggggg
1141 agaaacaaac aaacaaacaa acaaacaaac aaaaaaccca agggctccag aagccaggct
1201 ggcaggtgca gctgggcggg gcaggtcaca gagagtcagg gacttgcccc agcatgcccc
1261 gaacgtccat gcccagccag ccttccgaga gggacacgcc atggcagcag aggctttcca
1321 caactcgctg cctctgctct tggcctgcag gtcagaggga ggctgcggct gtggagggca
1381 ctagaggaac ttggcccaga aagagcctca gagtcctagt ggtgggggcc tggaaggacc
1441 agggcccctt cgcagggtca tcctgccagg gctcagccca ccttggctac gacacagccc
1501 acaccggcct ggttaactgg gcttcatccc agcatcaggc actccaaggg gcaggggctt
1561 ctcttcggtg ctccccagca gggtctctgg cgtctccagc tgtgaccgaa aggcacattc
1621 ctccttggag aaggggacct gctcgtcctt cggggactcc gaggggctgg aatctgtgtc
1681 tcccattgtg gagctggctt gggaggagca ctgtgagctg tggtcagagc tgctacagac
1741 gttcacgatg caggtgacat tgacctgggt cccatggcca ccaggggaag aatctgagct
1801 cccggtgctg gcccgggcct ccccggcccc actggcctcc acgcctggtg cctgtggctg
1861 gttccgagtg ggcgcccttc tgtccaacgc actggccgag ctctccaggg agctgctgct
1921 ggagctcggc gctgtgatca gcaggtgctg ctgctcgggg ccctgtgtac cccgggcctt
1981 atcggcaggc aagtgaggca ccttggcttc tctctgcagg cacaagggct tctttttcac
2041 ctgggtcatg atgacacagt tcaccactcc tattattagt agacccaagg ctgtcacacc
2101 cacaatcagt ccaactggaa gagcgaagtc gccagtgctc ccttcagctg gggggctggg
2161 gcccattggg agcaggaagg aggtgcttgg agcagtgctg ggttctggag ttggctgcgt
2221 gtgttgggat cgtgtggaca ctggctgggg taagtgtact gcccctgggg ccatactccg
2281 ggtgggggac gtggacgtgc agactgcatc catgcttgca ttcccaggga tggccaccac
2341 gttacagatc tggtggggcc tgcaaatatc cgtggatgaa gtcgtgttgg agaacgtccc
2401 cggggcacag ggcttgcaca ccacgtctga tgtttcagtt cctggtctgg ccacgccgaa
2461 gcccgggcgg cacttgcgca gcggcgcgca cagccggcac ccctcctgct tgctcagcgc
2521 gcagtaccag ccgggcctgc aggtgcagat gcggttctgt tcccgagtgc aggcttgagt
2581 ttccacctgg tcagagctac agcgggagcc acagctcaag cactcgggaa cccagttcca
2641 gagctgggtg tatgtgctgt cctcacagga gtcacacacg gtgtccgagg tcttggtaca
2701 gaagactttt gcatgttggc ccggcgagca tttgctgcag cacatctgag ctgtctggtc
2761 atagtattct ctgagccggc atgtgctccc gggctccggg gcgngaattc gagctcggta
2821 cccggggatc ctctagagtc gacctgcagg catgcaagct tggcgtaatc atggtcatag
2881 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc
2941 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
3001 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
3061 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
3121 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
3181 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
3241 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
3301 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
3361 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
3421 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
3481 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
3541 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
3601 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
3661 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca
3721 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
3781 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
3841 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
3901 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
3961 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
4021 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
4081 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
4141 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
4201 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
4261 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
4321 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
4381 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
4441 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
4501 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
4561 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
4621 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
4681 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
4741 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
4801 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
4861 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
4921 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
4981 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc
5041 attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtc
//