LOCUS LT727528 5135 bp DNA circular SYN 06-FEB-2017
DEFINITION Vector pBluMTNFR55, complete sequence.
ACCESSION LT727528
VERSION LT727528.1
KEYWORDS .
SOURCE Vector pBluMTNFR55
ORGANISM Vector pBluMTNFR55
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 5135)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..5135
/organism="Vector pBluMTNFR55"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 2559."
/db_xref="taxon:1945258"
rep_origin 4..459
/note="f1 ori"
regulatory 626..642
/note="T7 promoter"
/regulatory_class="promoter"
misc_feature complement(761..1249)
/note="3' UTR of mTNFR55"
CDS complement(1250..>2527)
/codon_start=1
/transl_table=11
/note="mTNFR55 mature sequence"
/protein_id="SJL88751.1"
/translation="LVPSLGDREKRDSLCPQGKYVHSKNNSICCTKCHKGTYLVSDCP
SPGRDTVCRECEKGTFTASQNYLRQCLSCKTCRKEMSQVEISPCQADKDTVCGCKENQ
FQRYLSETHFQCVDCSPCFNGTVTIPCKETQNTVCNCHAGFFLRESECVPCSHCKKNE
ECMKLCLPPPLANVTNPQDSGTAVLLPLVILLGLCLLSFIFISLMCRYPRWRPEVYSI
ICRDPVPVKEEKAGKPLTPAPSPAFSPTSGFNPTLGFSTPGFSSPVSSTPISPIFGPS
NWHFMPPVSEVVPTQGADPLLYESLCSVPAPTSVQKWEDSAHPQRPDNADLAILYAVV
DGVPPARWKEFMRFMGLSEHEIERLEMQNGRCLREAQYSMLEAWRRRTPRHEDTLEVV
GLVLSKMNLAGCLENILEALRNPAPSSTTRLPR"
CDS complement(<2528..2614)
/transl_table=11
/note="mTNFR55 signal sequence"
/protein_id="SJL88752.1"
/translation="MGLPTVPGLLLSLVLLALLMGIHPSGVTG"
misc_feature complement(2615..2883)
/note="5' UTR of mTNFR55"
regulatory complement(2949..2965)
/note="T3 promoter"
/regulatory_class="promoter"
regulatory complement(3029..3112)
/note="lac promoter"
/regulatory_class="promoter"
rep_origin complement(3204..3606)
/note="pMB1 ori"
CDS complement(4147..5007)
/transl_table=11
/note="ampicillin"
/protein_id="SJL88753.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
BASE COUNT 1189 a 1320 c 1362 g 1264 t
ORIGIN
1 ctgacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
61 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
121 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
181 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
241 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
301 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
361 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
421 ttaacgcgaa ttttaacaaa atattaacgc ttacaatttc cattcgccat tcaggctgcg
481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tggagctcca
661 ccgcggtggc ggccgctcta gaactagtgg atcccccggg ctgcaggaat tccttttttt
721 tttttttttt tttttttttt tttttttttt ttttttttat catttttaga cgtttagtgt
781 atttacaaga gtcgagggct ccagctggag accccgcctt tctatgcttg tccttacctt
841 acacagttca tccattagtg tgatataatt gatacaaaga ttgcttatca aaagtgaaaa
901 aggcccagaa acccactgag gaggccctga gaagcttggc ttgggccctg tgcatcccaa
961 gtgggcacca gatacattgt gcctttcctc tacaatcctg tctttggcac cctcagctgg
1021 ccaggagctg atggtacatc tccctgccac tcacaagtgg ctggctcagg cagtcctcgg
1081 cggctgagaa aagctatgta caccaagtcg gtagcaccaa ggaagtggct accagctcga
1141 gccttccccc ttagagacct ttgcccactt ttcacccaca gggagtaggg catctagcag
1201 gatggtcctt gaagttcaag tccctcttcc taaggttgtg ggtgtggctt tatcgcggga
1261 ggcgggtcgt ggacgagggg gcgggatttc tcagagcctc gaggatattc tccaggcacc
1321 cagccaggtt catcttggaa agcacgaggc ccactacttc cagcgtgtcc tcgtggcgcg
1381 gcgtgcggcg ccgccaggct tccagcatgc tgtactgagc ctcgcgcagg cagcgcccgt
1441 tctgcatctc cagcctctcg atctcgtgct cgctcagccc catgaaacgc atgaactcct
1501 tccagcgcgc tggaggcacg ccatccacca cagcatacag aatcgcaagg tctgcattgt
1561 caggacgttg cgggtgggcg gagtcttccc atttctgaac agaggtgggg gctggcacgg
1621 agcagagtga ttcgtagagc agagggtcag ctccctgggt tgggactacc tcactgacag
1681 gtggcatgaa gtgccagtta ctaggaccga agatggggct gatgggggta ctggagacag
1741 gagaactaaa gcctggggtg ctgaagccca gagtggggtt gaagccggag gtggggctga
1801 aggctgggga gggggctgga gttaggggct ttccagcctt ctcctctttg acaggcacgg
1861 gatccctaca aatgatggag tagacttcgg gcctccaccg gggatatcgg cacattaaac
1921 tgatgaagat aaaggataga aggcaaagac ctagcaagat aaccaggggc aacagcaccg
1981 cagtacctga gtcctggggg tttgtgacat ttgcaagcgg aggaggtagg cacaacttca
2041 tacactcctc atttttcttg cagtggctgc aagggacgca ctcactttct ctcagaaaga
2101 accctgcatg gcagttacac acggtgttct gagtctcctt acaggggatt gtcacggtgc
2161 cgttgaagca ggggctgcag tccacgcact ggaagtgtgt ctcactcagg tagcgttgga
2221 actggttctc cttacagcca cacaccgtgt ccttgtcagc ttggcaagga gagatctcca
2281 cctgggacat ttctttccga catgtcttgc aactgagaca ctgcctgagg taattctggg
2341 aagccgtaaa ggtgcccttt tcacactccc tgcagactgt atcccgccct gggctcggac
2401 agtcactcac caagtaggtt cctttgtggc acttggtgca gcagatggaa ttgttcttag
2461 aatggacata ctttccttgg ggacacaagc tatccctctt ctcccggtca ccaagagaag
2521 ggactagtcc agtgacccct gatggatgta tccccatcag cagagccagg agcaccagtg
2581 acagcagcag gccaggcacg gtggggagac ccatgtccgg ccggcagtgg cctctcactc
2641 ccccattggg gctggggaca gggcagcaat tgacaacgct cgtgaatgaa gtaagatgat
2701 cggaccaggt ggcccgggcg atggcagcct gggcctcgag ttcgagaagc tgaaagtcag
2761 aggcagatag agatcagggt acggtcctcc aggacctcgc acccagtagg cccgatcatg
2821 agccagagtt cggaaaactc ggagaaaatt taagctgagg ggagagagaa ggtaggagcg
2881 gaattcgata tcaagcttat cgataccgtc gacctcgagg gggggcccgg tacccagctt
2941 ttgttccctt tagtgagggt taattgcgcg cttggcgtaa tcatggtcat agctgtttcc
3001 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
3061 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
3121 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg
3181 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
3241 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
3301 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
3361 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
3421 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
3481 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
3541 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
3601 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
3661 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
3721 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
3781 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg
3841 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
3901 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
3961 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
4021 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
4081 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
4141 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
4201 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
4261 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
4321 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
4381 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
4441 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
4501 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
4561 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
4621 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
4681 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
4741 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
4801 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
4861 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
4921 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
4981 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
5041 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
5101 aggggttccg cgcacatttc cccgaaaagt gccac
//