LOCUS LT727524 5310 bp DNA circular SYN 06-FEB-2017
DEFINITION Vector pATHTNF, complete sequence.
ACCESSION LT727524
VERSION LT727524.1
KEYWORDS .
SOURCE Vector pATHTNF
ORGANISM Vector pATHTNF
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 5310)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..5310
/organism="Vector pATHTNF"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 2529."
/db_xref="taxon:1945246"
CDS 86..1276
/transl_table=11
/note="tetracycline resistance"
/protein_id="SJL88733.1"
/translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASH
YGVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAG
RIVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLH
APFLAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTV
FFIMQLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGE
KQAIIAGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQG
QLQGSLAALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSR
ATST"
rep_origin complement(1647..2049)
/note="pMB1 ori"
misc_feature 2939..3086
/note="5' UTR of hTNF"
CDS 3087..>3314
/transl_table=11
/note="hTNF presequence"
/protein_id="SJL88734.1"
/translation="MSTESMIRDVELAEEALPKKTGGPQGSRRCLFLSLFSFLIVAGA
TTLFCLLHFGVIGPQREEFPRDLSLISPLAQA"
CDS <3315..3788
/codon_start=1
/transl_table=11
/note="mature hTNF"
/protein_id="SJL88735.1"
/translation="VRSSSRTPSDKPVAHVVANPQAEGQLQWLNRRANALLANGVELR
DNQLVVPSEGLYLIYSQVLFKGQGCPSTHVLLTHTISRIAVSYQTKVNLLSAIKSPCQ
RETPEGAEAKPWYEPIYLGGVFQLEKGDRLSAEINRPDYLDFAESGQVYFGIIAL"
misc_feature 3789..4526
/note="3' UTR of hTNF"
BASE COUNT 1170 a 1527 c 1367 g 1246 t
ORIGIN
1 ttctcatgtt tgacagctta tcatcgataa gctttaatgc ggtagtttat cacagttaaa
61 ttgctaacgc agtcaggcac cgtgtatgaa atctaacaat gcgctcatcg tcatcctcgg
121 caccgtcacc ctggatgctg taggcatagg cttggttatg ccggtactgc cgggcctctt
181 gcgggatatc gtccattccg acagcatcgc cagtcactat ggcgtgctgc tagcgctata
241 tgcgttgatg caatttctat gcgcacccgt tctcggagca ctgtccgacc gctttggccg
301 ccgcccagtc ctgctcgctt cgctacttgg agccactatc gactacgcga tcatggcgac
361 cacacccgtc ctgtggatcc tctacgccgg acgcatcgtg gccggcatca ccggcgccac
421 aggtgcggtt gctggcgcct atatcgccga catcaccgat ggggaagatc gggctcgcca
481 cttcgggctc atgagcgctt gtttcggcgt gggtatggtg gcaggccccg tggccggggg
541 actgttgggc gccatctcct tgcatgcacc attccttgcg gcggcggtgc tcaacggcct
601 caacctacta ctgggctgct tcctaatgca ggagtcgcat aagggagagc gtcgaccgat
661 gcccttgaga gccttcaacc cagtcagctc cttccggtgg gcgcggggca tgactatcgt
721 cgccgcactt atgactgtct tctttatcat gcaactcgta ggacaggtgc cggcagcgct
781 ctgggtcatt ttcggcgagg accgctttcg ctggagcgcg acgatgatcg gcctgtcgct
841 tgcggtattc ggaatcttgc acgccctcgc tcaagccttc gtcactggtc ccgccaccaa
901 acgtttcggc gagaagcagg ccattatcgc cggcatggcg gccgacgcgc tgggctacgt
961 cttgctggcg ttcgcgacgc gaggctggat ggccttcccc attatgattc ttctcgcttc
1021 cggcggcatc gggatgcccg cgttgcaggc catgctgtcc aggcaggtag atgacgacca
1081 tcagggacag cttcaaggat cgctcgcggc tcttaccagc ctaacttcga tcactggacc
1141 gctgatcgtc acggcgattt atgccgcctc ggcgagcaca tggaacgggt tggcatggat
1201 tgtaggcgcc gccctatacc ttgtctgcct ccccgcgttg cgtcgcggtg catggagccg
1261 ggccacctcg acctgaatgg aagccggcgg cacctcgcta acggattcac cactccaaga
1321 attggagcca atcaattctt gcggagaact gtgaatgcgc aaaccaaccc ttggcagaac
1381 atatccatcg cgtccgccat ctccagcagc cgcacgcggc gcatctcggg cagcgttggg
1441 tcctggccac gggtgcgcat gatcgtgctc ctgtcgttga ggacccggct aggctggcgg
1501 ggttgcctta ctggttagca gaatgaatca ccgatacgcg agcgaacgtg aagcgactgc
1561 tgctgcaaaa cgtctgcgac ctgagcaaca acatgaatgg tcttcggttt ccgtgtttcg
1621 taaagtctgg aaacgcggaa gtcagcgctc ttccgcttcc tcgctcactg actcgctgcg
1681 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
1741 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
1801 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
1861 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
1921 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
1981 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
2041 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
2101 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
2161 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
2221 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt
2281 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
2341 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
2401 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
2461 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
2521 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
2581 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
2641 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
2701 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
2761 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
2821 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
2881 tttgcgcaac gttgttgcca ttgctgcagg gggggggggg gggggggggg ggggggggag
2941 gaccagctaa gagggagaga agcaactaca gaccccccct gaaaacaacc ctcagacgcc
3001 acatcccctg acaagctgcc aggcaggttc tcttcctctc acatactgac ccacggctcc
3061 accctctctc ccctggaaag gacaccatga gcactgaaag catgatccgg gacgtggagc
3121 tggccgagga ggcgctcccc aagaagacag gggggcccca gggctccagg cggtgcttgt
3181 tcctcagcct cttctccttc ctgatcgtgg caggcgccac cacgctcttc tgcctgctgc
3241 actttggagt gatcggcccc cagagggaag agttccccag ggacctctct ctaatcagcc
3301 ctctggccca ggcagtcaga tcatcttctc gaaccccgag tgacaagcct gtagcccatg
3361 ttgtagcaaa ccctcaagct gaggggcagc tccagtggct gaaccgccgg gccaatgccc
3421 tcctggccaa tggcgtggag ctgagagata accagctggt ggtgccatca gagggcctgt
3481 acctcatcta ctcccaggtc ctcttcaagg gccaaggctg cccctccacc catgtgctcc
3541 tcacccacac catcagccgc atcgccgtct cctaccagac caaggtcaac ctcctctctg
3601 ccatcaagag cccctgccag agggagaccc cagagggggc tgaggccaag ccctggtatg
3661 agcccatcta tctgggaggg gtcttccagc tggagaaggg tgaccgactc agcgctgaga
3721 tcaatcggcc cgactatctc gactttgccg agtctgggca ggtctacttt gggatcattg
3781 ccctgtgagg aggacgaaca tccaaccttc ccaaacgcct cccctgcccc aatcccttta
3841 ttaccccctc cttcagacac cctcaacctc ttctggctca aaaagagaat tgggggctta
3901 gggtcggaac ccaagcttag aactttaagc aacaagacca ccacttcgaa acctgggatt
3961 caggaatgtg tggcctgcac agtgaattgc tggcaaccac taagaattca aactggggcc
4021 tccagaactc actggggcct acagctttga tccctgacat ctggaatctg gagaccaggg
4081 agcctttggt tctggccaga atgctgcagg acttgagaag acctcaccta gaaattgaca
4141 caagtggacc ttaggccttc ctctctccag atgtttccag acttccttga gacacggagc
4201 ccagccctcc ccatggagcc agctccctct atttatgttt gcacttgtga ttatttatta
4261 tttatttatt atttatttat ttacagatga atgtatttat ttgggagacc ggggtatcct
4321 gggggaccca atgtaggagc tgccttggct cagacatgtt ttccgtgaaa acggagctga
4381 acaataggct gttcccatgt agccccctgg cctctgtgcc ttcttttgat tatgtttttt
4441 aaaatattta tctgattaag ttgtctaaac aatgctgatt tggtgaccaa ctgtcactca
4501 ttgctgagcc tctgctcccc aggggacccc cccccccccc cccccccccc cccccctgca
4561 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
4621 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
4681 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
4741 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
4801 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaaca
4861 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
4921 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
4981 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
5041 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
5101 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
5161 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
5221 aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg
5281 cgtatcacga ggccctttcg tcttcaagaa
//