LOCUS LT727523 5208 bp DNA circular SYN 06-FEB-2017
DEFINITION Vector pATMTNF1, complete sequence.
ACCESSION LT727523
VERSION LT727523.1
KEYWORDS .
SOURCE Vector pATMTNF1
ORGANISM Vector pATMTNF1
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 5208)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..5208
/organism="Vector pATMTNF1"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 2527."
/db_xref="taxon:1945251"
CDS 86..1276
/transl_table=11
/note="tetracyclin resistance"
/protein_id="SJL88731.1"
/translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASH
YGVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAG
RIVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLH
APFLAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTV
FFIMQLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGE
KQAIIAGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQG
QLQGSLAALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSR
ATST"
rep_origin complement(1647..2049)
/note="pMB1 ori"
misc_feature 2939..2942
/note="5UTR"
misc_feature 2943..3179
/note="pmTNF"
CDS <3180..3650
/codon_start=1
/transl_table=11
/note="mTNF"
/protein_id="SJL88732.1"
/translation="LRSSSQNSSDKPVAHVVANHQVEEQLEWLSQRANALLANGMDLK
DNQLVVPADGLYLVYSQVLFKGQGCPDYVLLTHTVSRFAISYQEKVNLLSAVKSPCPK
DTPEGAELKPWYEPIYLGGVFQLEKGDQLSAEVNLPKYLDFAESGQVYFGVIAL"
misc_feature 3651..4424
/note="3UTR"
BASE COUNT 1163 a 1466 c 1321 g 1258 t
ORIGIN
1 ttctcatgtt tgacagctta tcatcgataa gctttaatgc ggtagtttat cacagttaaa
61 ttgctaacgc agtcaggcac cgtgtatgaa atctaacaat gcgctcatcg tcatcctcgg
121 caccgtcacc ctggatgctg taggcatagg cttggttatg ccggtactgc cgggcctctt
181 gcgggatatc gtccattccg acagcatcgc cagtcactat ggcgtgctgc tagcgctata
241 tgcgttgatg caatttctat gcgcacccgt tctcggagca ctgtccgacc gctttggccg
301 ccgcccagtc ctgctcgctt cgctacttgg agccactatc gactacgcga tcatggcgac
361 cacacccgtc ctgtggatcc tctacgccgg acgcatcgtg gccggcatca ccggcgccac
421 aggtgcggtt gctggcgcct atatcgccga catcaccgat ggggaagatc gggctcgcca
481 cttcgggctc atgagcgctt gtttcggcgt gggtatggtg gcaggccccg tggccggggg
541 actgttgggc gccatctcct tgcatgcacc attccttgcg gcggcggtgc tcaacggcct
601 caacctacta ctgggctgct tcctaatgca ggagtcgcat aagggagagc gtcgaccgat
661 gcccttgaga gccttcaacc cagtcagctc cttccggtgg gcgcggggca tgactatcgt
721 cgccgcactt atgactgtct tctttatcat gcaactcgta ggacaggtgc cggcagcgct
781 ctgggtcatt ttcggcgagg accgctttcg ctggagcgcg acgatgatcg gcctgtcgct
841 tgcggtattc ggaatcttgc acgccctcgc tcaagccttc gtcactggtc ccgccaccaa
901 acgtttcggc gagaagcagg ccattatcgc cggcatggcg gccgacgcgc tgggctacgt
961 cttgctggcg ttcgcgacgc gaggctggat ggccttcccc attatgattc ttctcgcttc
1021 cggcggcatc gggatgcccg cgttgcaggc catgctgtcc aggcaggtag atgacgacca
1081 tcagggacag cttcaaggat cgctcgcggc tcttaccagc ctaacttcga tcactggacc
1141 gctgatcgtc acggcgattt atgccgcctc ggcgagcaca tggaacgggt tggcatggat
1201 tgtaggcgcc gccctatacc ttgtctgcct ccccgcgttg cgtcgcggtg catggagccg
1261 ggccacctcg acctgaatgg aagccggcgg cacctcgcta acggattcac cactccaaga
1321 attggagcca atcaattctt gcggagaact gtgaatgcgc aaaccaaccc ttggcagaac
1381 atatccatcg cgtccgccat ctccagcagc cgcacgcggc gcatctcggg cagcgttggg
1441 tcctggccac gggtgcgcat gatcgtgctc ctgtcgttga ggacccggct aggctggcgg
1501 ggttgcctta ctggttagca gaatgaatca ccgatacgcg agcgaacgtg aagcgactgc
1561 tgctgcaaaa cgtctgcgac ctgagcaaca acatgaatgg tcttcggttt ccgtgtttcg
1621 taaagtctgg aaacgcggaa gtcagcgctc ttccgcttcc tcgctcactg actcgctgcg
1681 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
1741 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
1801 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
1861 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
1921 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
1981 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
2041 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
2101 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
2161 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
2221 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt
2281 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
2341 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
2401 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
2461 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
2521 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
2581 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
2641 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
2701 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
2761 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
2821 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
2881 tttgcgcaac gttgttgcca ttgctgcagg gggggggggg gggggggggg ggggggggca
2941 ccatgagcac agaaagcatg atccgcgacg tggaactggc agaagaggca ctcccccaaa
3001 agatgggggg cttccagaac tccaggcggt gcctatgtct cagcctcttc tcattcctgc
3061 ttgtggcagg ggccaccacg ctcttctgtc tactgaactt cggggtgatc ggtccccaaa
3121 gggatgagaa gttcccaaat ggcctccctc tcatcagttc tatggcccag accctcacac
3181 tcagatcatc ttctcaaaat tcgagtgaca agcctgtagc ccacgtcgta gcaaaccacc
3241 aagtggagga gcagctggag tggctgagcc agcgcgccaa cgccctcctg gccaacggca
3301 tggatctcaa agacaaccaa ctagtggtgc cagccgatgg gttgtacctt gtctactccc
3361 aggttctctt caagggacaa ggctgccccg actacgtgct cctcacccac accgtcagcc
3421 gatttgctat ctcataccag gagaaagtca acctcctctc tgccgtcaag agcccctgcc
3481 ccaaggacac ccctgagggg gctgagctca aaccctggta tgagcccata tacctgggag
3541 gagtcttcca gctggagaag ggggaccaac tcagcgctga ggtcaatctg cccaagtact
3601 tagactttgc ggagtccggg caggtctact ttggagtcat tgctctgtga agggaatggg
3661 tgttcatcca ttctctaccc agcccccact ctgacccctt tactctgacc cctttattgt
3721 ctactcctca gagcccccag tctgtgtcct tctaacttag aaaggggatt atggctcaga
3781 gtccaactct gtgctcagag ctttcaacaa ctactcagaa acacaagatg ctgggacagt
3841 gacctggact gtgggcctct catgcaccac catcaaggac tcaaatgggc tttccgaatt
3901 cactggagcc tcgaatgtcc attcctgagt tctgcaaagg gagagtggtc aggttgcctc
3961 tgtctcagaa tgaggctgga taagatctca ggccttccta ccttcagacc tttccagact
4021 cttccctgag gtgcaatgca cagccttcct cacagagcca gcccccctct atttatattt
4081 gcacttatta tttattattt atttattatt tatttatttg cttatgaatg tatttatttg
4141 gaaggccggg gtgtcctgga ggacccagtg tgggaagctg tcttcagaca gacatgtttt
4201 ctgtgaaaac ggagctgagc tgtccccacc tggcctctct accttgttgc ctcctctttt
4261 gcttatgttt aaaacaaaat atttatctaa cccaattgtc ttaataacgc tgatttggtg
4321 accaggctgt cgctacatca ctgaacctct gctccccacg ggagccgtga ctgtaattgc
4381 cctacagtca attgagagaa ataaacatcg cttaaaataa aaaacccccc cccccccccc
4441 cccccccccc cccctgcagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
4501 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
4561 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
4621 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
4681 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
4741 tcttgcccgg cgtcaacacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
4801 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
4861 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
4921 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
4981 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
5041 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
5101 ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca
5161 ttaacctata aaaataggcg tatcacgagg ccctttcgtc ttcaagaa
//