LOCUS LT727521 9906 bp DNA circular SYN 06-FEB-2017 DEFINITION Yeast expression vector pSCGAL10-SN, complete sequence. ACCESSION LT727521 VERSION LT727521.1 KEYWORDS . SOURCE Yeast expression vector pSCGAL10-SN ORGANISM Yeast expression vector pSCGAL10-SN other sequences; artificial sequences; vectors. REFERENCE 1 (bases 1 to 9906) AUTHORS De Schamphelaire W. JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent, Technologiepark 927, 9052, BELGIUM. REFERENCE 2 AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K., Roggeman Fonseca M., Vanhoucke M., Beyaert R. TITLE BCCM/LMBP Plasmid collection JOURNAL Unpublished. FEATURES Location/Qualifiers source 1..9906 /organism="Yeast expression vector pSCGAL10-SN" /mol_type="other DNA" /note="BCCM/LMBP Plasmid collection (Ghent University,Belgium) accession number LMBP 2471." /db_xref="taxon:1945497" rep_origin complement(754..1209) /note="f1 ori" CDS complement(1977..3071) /transl_table=11 /note="defective LEU2" /protein_id="SJL88724.1" /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENH LIGGAAIDATGVPLPDEALEASKKVDAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQL YANLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSE QYTVPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLK VQHQLIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPD KNTAFGLYEPCHGSAPDLPKNKVDPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLD AGIRTGDLGGSNSTTEVGDAVAEEVKKILA" rep_origin complement(3429..4409) /note="2mu ori" misc_feature 4176..4778 /note="2mu inverted repeat" regulatory complement(4491..4595) /note="FLP terminator" /regulatory_class="terminator" regulatory complement(4781..5051) /note="CYC1 terminator" /regulatory_class="terminator" misc_feature complement(5114..5313) /note="3' UTR of hIFNbeta" CDS complement(5314..5877) /transl_table=11 /note="hIFNbeta" /protein_id="SJL88725.1" /translation="MTNKCLLQIALLLCFSTTALSMSYNLLGFLQRSSNFQCQKLLWQ LNGRLEYCLKDRMNFDIPEEIKQLQQFQKEDAALTIYEMLQNIFAIFRQDSSSTGWNE TIVENLLANVYHQINHLKTVLEEKLEKEDFTRGKLMSSLHLKRYYGRILHYLKAKEYS HCAWTIVRVEILRNFYFINRLTGYLRN" regulatory complement(6015..6422) /note="GAL10/CYC1 promoter" /regulatory_class="promoter" CDS complement(6641..7444) /transl_table=11 /note="URA3" /protein_id="SJL88726.1" /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKEL LELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVK LQYSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLS TGEYTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYR TVDDVVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN" rep_origin complement(7899..8301) /note="pMB1 ori" CDS complement(8841..9701) /transl_table=11 /note="ampicillin resistance" /protein_id="SJL88727.1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" BASE COUNT 2820 a 2180 c 2126 g 2769 t ORIGIN 1 ctgcctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac 61 ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc 121 gggtgttggc gggtgtcggg gcgcagccat nnnccannna cgtagcgata gcggagtnta 181 tactggctta actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt 241 gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggnaattcca acatccaata 301 aatcatacag gcaaggcaaa gaattagcaa aattaagcaa taaagcctca gagcataaag 361 ctaaatcggt tgtaccaaaa acattatgac cctgtaatac ttttgcggga gaagccttta 421 tttcaacgca aggataaaaa tttttagaac cctcatatat tttaaatgca atgcctgagt 481 aatgtgtagg taaagattca aacgggtgag aaaggccgga gacagtcaaa tcaccatcaa 541 tatgatattc aaccgttcta gctgataaat tcatgccgga gagggtagct atttttgaga 601 ggtctacaaa ggctatcagg tcattgcctg agagtctgga gcaaacaaga gaatcgatga 661 acggtaatcg taaaactagc atgtcaatca tatgtacccc ggttgataat cagaaaagcc 721 ccaaaaacag gaagattgta taagcaaata tttaaattgt aaacgttaat attttgttaa 781 aattcgcgtt aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca 841 aaatccctta taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga 901 acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc 961 agggcgatgg cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc 1021 gtaaagcact aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc 1081 cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg 1141 caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac 1201 agggcgcgta ctatggttgc tttgacgagc acgtataacg tgctttcctc gttagaatca 1261 gagcgggagc taaacaggag gccgattaaa gggattttag acaggaacgg tacgccagaa 1321 tcctgagaag tgtttttata atcagtgagg ccaccgagta aaagagtctg tccatcacgc 1381 aaattaaccg ttgtcgcaat acttctttga ttagtaataa catcacttgc ctgagtagaa 1441 gaactcaaac tatcggcctt gctggtaata tccagaacaa tattaccgcc agccattgca 1501 acaggaaaaa cgctcatgga aatacctaca ttttgacgct caatcgtctg gaattnccat 1561 tcgccattca ggctacgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta 1621 cgccanctgg cgaagggggg atgtgctgca aggcgattaa gttgggtaac gccagggttt 1681 tcccagtcag tacgttgtaa aacgacggcc agtgccaagc tccttaccac tcttttgtta 1741 ctctattgat ccagctcagc aaaggcagtg tgatctaaga ttctatcttc gcgatgtagt 1801 aaaactagct agaccgagaa agagactaga aatgcaaaag gcacttctac aatggctgcc 1861 atcattatta tccgatgtga cgctgcattt tttttttttt gtaatttcgt gtcgtttcta 1921 ttatgaattt catttataaa gtttatgtac aaatatcata aaaaaagaga atctttttaa 1981 gcaaggattt tcttaacttc ttcggcgaca gcatcaccga cttcggtggt actgttggaa 2041 ccacctaaat caccagttct gatacctgca tccaaaacct ttttaactgc atcttcaatg 2101 gccttacctt cttcaggcaa gttcaatgac aatttcaaca tcattgcagc agacaagata 2161 gtggcgatag ggtcaacctt attctttggc aaatctggag cagaaccatg gcatggttcg 2221 tacaaaccaa atgcggtgtt cttgtctggc aaagaggcca aggacgcaga tggcaacaaa 2281 cccaaggaac ctgggataac ggaggcttca tcggagatga tatcaccaaa catgttgctg 2341 gtgattataa taccatttag gtgggttggg ttcttaacta ggatcatggc ggcagaatca 2401 atcaattgat gttgaacctt caatgtaggg aattcgttct tgatggtttc ctccacagtt 2461 tttctccata atcttgaaga ggccaaaaca ttagctttat ccaaggacca aataggcaat 2521 ggtggctcat gttgtagggc catgaaagcg gccattcttg tgattctttg cacttctgga 2581 acggtgtatt gttcactatc ccaagcgaca ccatcaccat cgtcttcctt tctcttacca 2641 aagtaaatac ctcccactaa ttctctgaca acaacgaagt cagtaccttt agcaaattgt 2701 ggcttgattg gagataagtc taaaagagag tcggatgcaa agttacatgg tcttaagttg 2761 gcgtacaatt gaagttcttt acggattttt agtaaacctt gttcaggtct aacactaccg 2821 gtaccccatt taggaccacc cacagcacct aacaaaacgg catcaacctt cttggaggct 2881 tccagcgcct catctggaag tgggacacct gtagcatcga tagcagcacc accaattaaa 2941 tgattttcga aatcgaactt gacattggaa cgaacatcag aaatagcttt aagaacctta 3001 atggcttcgg ctgtgatttc ttgaccaacg tggtcacctg gcaaaacgac gatcttctta 3061 ggggcagaca ttacaatggt atatccttga aatatatata aaaaaaaaaa aaaatgcagc 3121 ttctcaatga tattcgaata cgctttgagg agatacagcc taatatccga caaactgttt 3181 tacagattta cgatcgtact tgttacccat cattgaattt tgaacatccg aacctgggag 3241 ttttccctga aacagatagt atatttgaac ctgtataata atatatagtc tagcgcttta 3301 cggaagacaa tgtatgtatt tcggttcctg gagaaactat tgcatctatt gcataggtaa 3361 tcttgcacgt cgcatccccg gttcattttc tgcgtttcca tcttgcactt caatagcata 3421 tctttgttaa cgaagcatct gtgcttcatt ttgtagaaca aaaatgcaac gcgagagcgc 3481 taatttttca aacaaagaat ctgagctgca tttttacaga acagaaatgc aacgcgaaag 3541 cgctatttta ccaacgaaga atctgtgctt catttttgta aaacaaaaat gcaacgcgag 3601 agcgctaatt tttcaaacaa agaatctgag ctgcattttt acagaacaga aatgcaacgc 3661 gagagcgcta ttttaccaac aaagaatcta tacttctttt ttgttctaca aaaatgcatc 3721 ccgagagcgc tatttttcta acaaagcatc ttagattact ttttttctcc tttgtgcgct 3781 ctataatgca gtctcttgat aactttttgc actgtaggtc cgttaaggtt agaagaaggc 3841 tactttggtg tctattttct cttccataaa aaaagcctga ctccacttcc cgcgtttact 3901 gattactagc gaagctgcgg gtgcattttt tcaagataaa ggcatccccg attatattct 3961 ataccgatgt ggattgcgca tactttgtga acagaaagtg atagcgttga tgattcttca 4021 ttggtcagaa aattatgaac ggtttcttct attttgtctc tatatactac gtataggaaa 4081 tgtttacatt ttcgtattgt tttcgattca ctctatgaat agttcttact acaatttttt 4141 tgtctaaaga gtaatactag agataaacat aaaaaatgta gaggtcgagt ttagatgcaa 4201 gttcaaggag cgaaaggtgg atgggtaggt tatataggga tatagcacag agatatatag 4261 caaagagata cttttgagca atgtttgtgg aagcggtatt cgcaatattt tagtagctcg 4321 ttacagtccg gtgcgttttt ggttttttga aagtgcgtct tcagagcgct tttggttttc 4381 aaaagcgctc tgaagttcct atactttcta gctagagaat aggaacttcg gaataggaac 4441 ttcaaagcgt ttccgaaaac gagcgcttcc gaaaatgcaa cgcgagctgc gcacatacag 4501 ctcactgttc acgtcgcacc tatatctgcg tgttgcctgt atatatatat acatgagaag 4561 aacggcatag tgcgtgttta tgcttaaatg cgtacttata tgcgtctatt tatgtaggat 4621 gaaaggtagt ctagtacctc ctgtgatatt atcccattcc atgcggggta tcgtatgctt 4681 ccttcagcac taccctttag ctgttctata tgctgccact cctcaattgg attagtctca 4741 tccttcaatg ctatcatttc ctttgatatt ggatcataag cttgcaaatt aaagccttcg 4801 agcgtcccaa aaccttctca agcaaggttt tcagtataat gttacatgcg tacacgcgtc 4861 tgtacagaaa aaaaagaaaa atttgaaata taaataacgt tcttaatact aacataacta 4921 taaaaaaata aatagggacc tagacttcag gttgtctaac tccttccttt tcggttagag 4981 cggatgtggg gggagggcgt gaatgtaagc gtgacataac taattacatg atatcgacaa 5041 aggaaaaggg ggacggatcc ccgggaattc gagctcgccc ggggatcctc taggcggccg 5101 ctaggcctcg agggactttt gcaccaaaaa taatttattt tccaaaataa aatttaaata 5161 aataaaaata actcataatt taataaaaat ttcaaaatct tctagtgtcc tttcatatgc 5221 agtacattag ccatcagtca cttaaacagc atctgctggt tgaagaatgc ttgaagcaat 5281 tgtccagtcc cagaggcaca ggctaggaga tcttcagttt cggaggtaac ctgtaagtct 5341 gttaatgaag taaaagttcc ttaggatttc cactctgact atggtccagg cacagtgact 5401 gtactccttg gccttcaggt aatgcagaat cctcccataa tatcttttca ggtgcagact 5461 gctcatgagt tttcccctgg tgaaatcttc tttctccagt ttttcttcca ggactgtctt 5521 cagatggttt atctgatgat agacattagc caggaggttc tcaacaatag tctcattcca 5581 gccagtgcta gatgaatctt gtctgaaaat agcaaagatg ttctggagca tctcatagat 5641 ggtcaatgcg gcgtcctcct tctggaactg ctgcagctgc ttaatctcct cagggatgtc 5701 aaagttcatc ctgtccttga ggcagtattc aagcctccca ttcaattgcc acaggagctt 5761 ctgacactga aaattgctgc ttctttgtag gaatccaagc aagttgtagc tcatggaaag 5821 agctgtagtg gagaagcaca acaggagagc aatttggagg agacacttgt tggtcatgtt 5881 cctcgaggcc tttttggcca agctgactct agaggatccc cgggtaccga gctcgaattg 5941 atccggtaat ttagtgtgtg tatttgtgtt tgcgtgtcta tagaagtata gtaatttatg 6001 ctacaaagga cctaatgtat aaggaaagaa tatttagaga aaagaagaaa acaagagttt 6061 tatatacata cagagcacat gcatgccata tgatcatgtg tcgtcgcaca catatatata 6121 tgcctgtatg tgtcagcact aaagttgcct ggccatccac gctatataca cgcctggcgg 6181 atctgctcga ggtcgattag cctaaaaaaa ccttctcttt ggaactttca gtaatacgct 6241 taactgctca ttgctatatt gaagtacgga ttagaagccg ccgagcgggt gacagccctc 6301 cgaaggaaga ctctcctccg tgcgtcctcg tcttcaccgg tcgcgttcct gaaacgcaga 6361 tgtgcctcgc gccgcactgc tccgaacaat aaagattcta caatactagc ttttatggtt 6421 atgaagagga aaaattggca gtaacctggc cccacaaacc ttcaaatgaa cgaatcaaat 6481 taacaaccat aggatgataa tgcgattagt tttttagcct tatttctggg gtaattaatc 6541 agcgaagcga tgatttttga tcgggtaata actgatataa ttaaattgaa gctctaattt 6601 gtgagtttag tatacatgca tttacttata atacagtttt ttagttttgc tggccgcatc 6661 ttctcaaata tgcttcccag cctgcttttc tgtaacgttc accctctacc ttagcatccc 6721 ttccctttgc aaatagtcct cttccaacaa taataatgtc agatcctgta gagaccacat 6781 catccacggt tctatactgt tgacccaatg cgtctccctt gtcatctaaa cccacaccgg 6841 gtgtcataat caaccaatcg taaccttcat ctcttccacc catgtctctt tgagcaataa 6901 agccgataac aaaatctttg tcgctcttcg caatgtcaac agtaccctta gtatattctc 6961 cagtagatag ggagcccttg catgacaatt ctgctaacat caaaaggcct ctaggttcct 7021 ttgttacttc ttctgccgcc tgcttcaaac cgctaacaat acctgggccc accacaccgt 7081 gtgcattcgt aatgtctgcc cattctgcta ttctgtatac acccgcagag tactgcaatt 7141 tgactgtatt accaatgtca gcaaattttc tgtcttcgaa gagtaaaaaa ttgtacttgg 7201 cggataatgc ctttagcggc ttaactgtgc cctccatgga aaaatcagtc aagatatcca 7261 catgtgtttt tagtaaacaa attttgggac ctaatgcttc aactaactcc agtaattcct 7321 tggtggtacg aacatccaat gaagcacaca agtttgtttg cttttcgtgc atgatattaa 7381 atagcttggc agcaacagga ctaggatgag tagcagcacg ttccttatat gtagctttcg 7441 acatgattta tcttcgtttc cggtttttgt tctgtgcagt tgggttaaga atactgggca 7501 atttcatgtt tcttcaacac tacatatgcg tatatatacc aatctaagtc tgtgctcctt 7561 ccttcgttct tccttctgtt cggagattac cgaatcaaaa aaatttcaaa gaaaccgaaa 7621 tcaaaaaaaa gaataaaaaa aaaatgatga attgaattga aaagctaatt cgtaatcatg 7681 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc 7741 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg aggtaactca cattaattgc 7801 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccanctgg attaatgaat 7861 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac 7921 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt 7981 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca 8041 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc 8101 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact 8161 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct 8221 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag 8281 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca 8341 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa 8401 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc 8461 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag 8521 aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg 8581 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca 8641 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc 8701 tgacgctcag tggaacgaaa actcacgtta gggattttgg tcatgagatt atcaaaaagg 8761 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat 8821 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc 8881 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg 8941 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct 9001 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca 9061 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg 9121 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg 9181 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc 9241 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag 9301 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg 9361 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag 9421 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat 9481 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg 9541 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca 9601 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca 9661 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat 9721 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag 9781 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa 9841 gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt 9901 cttcaa //