LOCUS LT727520 3198 bp DNA circular SYN 06-FEB-2017 DEFINITION Bacterial expression vector pPLc3, complete sequence. ACCESSION LT727520 VERSION LT727520.1 KEYWORDS . SOURCE Bacterial expression vector pPLc3 ORGANISM Bacterial expression vector pPLc3 other sequences; artificial sequences; vectors. REFERENCE 1 (bases 1 to 3198) AUTHORS De Schamphelaire W. JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent, Technologiepark 927, 9052, BELGIUM. REFERENCE 2 AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K., Roggeman Fonseca M., Vanhoucke M., Beyaert R. TITLE BCCM/LMBP Plasmid collection JOURNAL Unpublished. FEATURES Location/Qualifiers source 1..3198 /organism="Bacterial expression vector pPLc3" /mol_type="other DNA" /note="BCCM/LMBP Plasmid collection (Ghent University,Belgium) accession number LMBP 2470." /db_xref="taxon:1944699" misc_feature 98..130 /note="5' UTR of MS2 polymerase, incl. RBS" CDS 131..682 /transl_table=11 /note="HBc" /protein_id="SJL88722.1" /translation="MDIDPYKEFGATVELLSFLPSDFFPSVRDLLDTASALYREALES PEHCSPHHTALRQAILCWGELMTLATWVGVNLEDPASRDLVVSYVNTNMGLKFRQLLW FHISCLTFGRETVIEYLVSFGVWIRTPPAYRPPNAPILSTLPETTVVRRRGRSPRRRT PSPRRRRSQSPRRRRSQSRESQC" regulatory 686..725 /note="synthetic polyA" /regulatory_class="terminator" rep_origin complement(781..1183) /note="pMB1 ori" CDS complement(1724..2584) /transl_table=11 /note="ampicillin resistance" /protein_id="SJL88723.1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" regulatory 2950..3080 /note="lambda PL promoter" /regulatory_class="promoter" BASE COUNT 809 a 818 c 773 g 798 t ORIGIN 1 ttccgactgc gagcttattg ttaaggcaat gcaaggtctc ctaaaagatg gaaacccgat 61 tccctcagca atcgcagcaa actccggcat ctactaatag acgccggcca ttcaaacatg 121 aggattaccc atggatatcg atccttataa agaattcgga gctactgtgg agttactctc 181 gtttctcccg agtgacttct ttccttcagt acgagacctt ctggataccg ccagcgcgct 241 gtatcgggaa gccttggagt ctcctgagca ctgcagccct caccatactg ccctcaggca 301 agcaattctt tgctgggggg agctcatgac tctggccacg tgggtgggtg ttaacttgga 361 agatccagct agcagggacc tggtagtcag ttatgtcaac actaatatgg gtttaaagtt 421 caggcaactc ttgtggtttc acattagctg cctcactttc ggccgagaaa cagttataga 481 atatttggtg tctttcggag tgtggatccg cactcctcca gcttataggc ctccgaatgc 541 ccctatcctg tcgacactcc cggagactac tgttgttaga cgtcgaggca ggtcacctag 601 aagaagaact ccttcgcctc gcaggcgaag gtctcaatcg ccgcggcgcc gaagatctca 661 atctcgggaa tctcaatgtt agtgaagctt ccgggccggc gataatacgc cggcccgttt 721 tttttcagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc 781 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg 841 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa 901 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg 961 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga 1021 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg 1081 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg 1141 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc 1201 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg 1261 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca 1321 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt 1381 ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag 1441 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg 1501 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc 1561 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt 1621 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt 1681 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca 1741 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg 1801 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac 1861 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg 1921 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc 1981 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta 2041 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac 2101 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc 2161 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac 2221 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact 2281 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa 2341 cacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt 2401 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca 2461 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa 2521 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac 2581 tcatactctt cctttttcaa tattatgtaa gcagacagtt ttattgttca tgatgatata 2641 tttttatctt gtgcaatgta acatcagaga ttttgagaca caacgtggct ttgttgaata 2701 aatcgaactt ttgctgagtt gaaggatcag atcacgcatc ttcccgacaa cgcagaccgt 2761 tccgtggcaa agcaaaagtt caaaatcacc aactggtcca cctacaacaa agctctcatc 2821 aaccgtggct ccctcacttt ctggctggat gatggggcga ttcaggcctg gtatgagtca 2881 gcaacacctt cttcacgagg cagacctcag cgccggtgat gccggccacg atgcgtccgg 2941 cgtagaggat ctctcaccta ccaaacaatg cccccctgca aaaaataaat tcatataaaa 3001 aacatacaga taaccatctg cggtgataaa ttatctctgg cggtgttgac ataaatacca 3061 ctggcggtga tactgagcac atcagcagga cgcactgacc accatgaagg tgacgctctt 3121 aaaattaagc cctgaagaag ggcagcattc aaagcagaag gctttggggt gtgtgatacg 3181 aaacgaagca ttggaata //