LOCUS LT727517 4252 bp DNA circular SYN 06-FEB-2017
DEFINITION Reporter vector pBLCAT6, complete sequence.
ACCESSION LT727517
VERSION LT727517.1
KEYWORDS .
SOURCE Reporter vector pBLCAT6
ORGANISM Reporter vector pBLCAT6
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 4252)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..4252
/organism="Reporter vector pBLCAT6"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 2452."
/db_xref="taxon:1945200"
CDS 90..749
/transl_table=11
/note="chloramphenicol resistance"
/protein_id="SJL88716.1"
/translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
A"
CDS <839..913
/codon_start=1
/transl_table=11
/note="part of SV40 small t-antigen"
/protein_id="SJL88717.1"
/translation="DLCEGTLLLWCDIIGQTTYRDLKL"
misc_feature 914..980
/note="intron of SV40 small t-antigen"
misc_feature 981..1532
/note="3' UTR of SV40 small t-antigen"
regulatory 1533..1629
/note="SV40 polyA early"
/regulatory_class="terminator"
regulatory complement(1533..1629)
/note="SV40 polyA late"
/regulatory_class="terminator"
regulatory complement(1768..1851)
/note="lac promoter"
/regulatory_class="promoter"
rep_origin complement(1943..2345)
/note="pMB1 ori"
CDS complement(2886..3746)
/transl_table=11
/note="ampicillin resistance"
/protein_id="SJL88718.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
regulatory 3878..3952
/note="SV40 polyA early"
/regulatory_class="terminator"
regulatory complement(3878..3952)
/note="SV40 polyA late"
/regulatory_class="terminator"
regulatory 4093..4189
/note="SV40 polyA early"
/regulatory_class="terminator"
regulatory complement(4093..4189)
/note="SV40 polyA late"
/regulatory_class="terminator"
BASE COUNT 1167 a 952 c 929 g 1204 t
ORIGIN
1 aagcttgcat gcctgcaggt cgactctaga ggatccagat ctggatctcg aggagcttgg
61 cgagattttc aggagctaag gaagctaaaa tggagaaaaa aatcactgga tataccaccg
121 ttgatatatc ccaatggcat cgtaaagaac attttgaggc atttcagtca gttgctcaat
181 gtacctataa ccagaccgtt cagctggata ttacggcctt tttaaagacc gtaaagaaaa
241 ataagcacaa gttttatccg gcctttattc acattcttgc ccgcctgatg aatgctcatc
301 cggaattccg tatggcaatg aaagacggtg agctggtgat atgggatagt gttcaccctt
361 gttacaccgt tttccatgag caaactgaaa cgttttcatc gctctggagt gaataccacg
421 acgatttccg gcagtttcta cacatatatt cgcaagatgt ggcgtgttac ggtgaaaacc
481 tggcctattt ccctaaaggg tttattgaga atatgttttt cgtctcagcc aatccctggg
541 tgagtttcac cagttttgat ttaaacgtgg ccaatatgga caacttcttc gcccccgttt
601 tcaccatggg caaatattat acgcaaggcg acaaggtgct gatgccgctg gcgattcagg
661 ttcatcatgc cgtttgtgat ggcttccatg tcggcagaat gcttaatgaa ttacaacagt
721 actgcgatga gtggcagggc ggggcgtaat ttttttaagg cagttattgg tgcccttaaa
781 cgcctggtgc tacgcctgaa taagtgataa taagcggatg aatggcagaa attcgccgga
841 tctttgtgaa ggaaccttac ttctgtggtg tgacataatt ggacaaacta cctacagaga
901 tttaaagctc taaggtaaat ataaaatttt taagtgtata atgtgttaaa ctactgattc
961 taattgtttg tgtattttag attccaacct atggaactga tgaatgggag cagtggtgga
1021 atgcctttaa tgaggaaaac ctgttttgct cagaagaaat gccatctagt gatgatgagg
1081 ctactgctga ctctcaacat tctactcctc caaaaaagaa gagaaaggta gaagacccca
1141 aggactttcc ttcagaattg ctaagttttt tgagtcatgc tgtgtttagt aatagaactc
1201 ttgcttgctt tgctatttac accacaaagg aaaaagctgc actgctatac aagaaaatta
1261 tggaaaaata ttctgtaacc tttataagta ggcataacag ttataatcat aacatactgt
1321 tttttcttac tccacacagg catagagtgt ctgctattaa taactatgct caaaaattgt
1381 gtacctttag ctttttaatt tgtaaagggg ttaataagga atatttgatg tatagtgcct
1441 tgactagaga tcataatcag ccataccaca tttgtagagg ttttacttgc tttaaaaaac
1501 ctcccacacc tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaacttg
1561 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
1621 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
1681 gtctggatcg atccccgggt accgagctcg aattcgtaat catggtcata gctgtttcct
1741 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
1801 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
1861 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
1921 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
1981 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
2041 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
2101 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
2161 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
2221 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
2281 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
2341 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
2401 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
2461 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
2521 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt
2581 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
2641 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
2701 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
2761 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
2821 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
2881 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
2941 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
3001 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
3061 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
3121 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
3181 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
3241 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
3301 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
3361 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
3421 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
3481 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
3541 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
3601 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
3661 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
3721 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
3781 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
3841 ggggttccgc gcacatttcc ccgaaaagtg ccacctgaac ttgtttattg cagcttataa
3901 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
3961 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga tcataatcag
4021 ccataccaca tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa
4081 cctgaaacat aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg
4141 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
4201 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggatct cg
//