LOCUS LT727516 4404 bp DNA circular SYN 06-FEB-2017
DEFINITION Reporter vector pBLCAT5, complete sequence.
ACCESSION LT727516
VERSION LT727516.1
KEYWORDS .
SOURCE Reporter vector pBLCAT5
ORGANISM Reporter vector pBLCAT5
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 4404)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..4404
/organism="Reporter vector pBLCAT5"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 2451."
/db_xref="taxon:1945199"
regulatory 39..143
/note="HSV-TK promoter"
/regulatory_class="promoter"
CDS 242..901
/transl_table=11
/note="chloramphenicol resistance"
/protein_id="SJL88713.1"
/translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
A"
CDS <991..1065
/codon_start=1
/transl_table=11
/note="part of SV40 small t-antigen"
/protein_id="SJL88714.1"
/translation="DLCEGTLLLWCDIIGQTTYRDLKL"
misc_feature 1066..1132
/note="intron of SV40 small t-antigen"
misc_feature 1133..1684
/note="3' UTR of SV40 small t-antigen"
regulatory 1685..1781
/note="SV40 polyA early"
/regulatory_class="terminator"
regulatory complement(1685..1781)
/note="SV40 polyA late"
/regulatory_class="terminator"
regulatory complement(1920..2003)
/note="lac promoter"
/regulatory_class="promoter"
rep_origin complement(2095..2497)
/note="pMB1 ori"
CDS complement(3038..3898)
/transl_table=11
/note="ampicillin resistance"
/protein_id="SJL88715.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
regulatory 4030..4104
/note="SV40 polyA early"
/regulatory_class="terminator"
regulatory complement(4030..4104)
/note="SV40 polyA late"
/regulatory_class="terminator"
regulatory 4245..4341
/note="SV40 polyA early"
/regulatory_class="terminator"
regulatory complement(4245..4341)
/note="SV40 polyA late"
/regulatory_class="terminator"
BASE COUNT 1196 a 1004 c 976 g 1228 t
ORIGIN
1 aagcttgcat gcctgcaggt cgactctaga ggatccggcc ccgcccagcg tcttgtcatt
61 ggcgaattcg aacacgcaga tgcagtcggg gcggcgcggt ccgaggtcca cttcgcatat
121 taaggtgacg cgtgtggcct cgaacaccga gcgaccctgc agcgacccgc ttaacagcgt
181 caacagcgtg ccgcagatct cgaggagctt ggcgagattt tcaggagcta aggaagctaa
241 aatggagaaa aaaatcactg gatataccac cgttgatata tcccaatggc atcgtaaaga
301 acattttgag gcatttcagt cagttgctca atgtacctat aaccagaccg ttcagctgga
361 tattacggcc tttttaaaga ccgtaaagaa aaataagcac aagttttatc cggcctttat
421 tcacattctt gcccgcctga tgaatgctca tccggaattc cgtatggcaa tgaaagacgg
481 tgagctggtg atatgggata gtgttcaccc ttgttacacc gttttccatg agcaaactga
541 aacgttttca tcgctctgga gtgaatacca cgacgatttc cggcagtttc tacacatata
601 ttcgcaagat gtggcgtgtt acggtgaaaa cctggcctat ttccctaaag ggtttattga
661 gaatatgttt ttcgtctcag ccaatccctg ggtgagtttc accagttttg atttaaacgt
721 ggccaatatg gacaacttct tcgcccccgt tttcaccatg ggcaaatatt atacgcaagg
781 cgacaaggtg ctgatgccgc tggcgattca ggttcatcat gccgtttgtg atggcttcca
841 tgtcggcaga atgcttaatg aattacaaca gtactgcgat gagtggcagg gcggggcgta
901 atttttttaa ggcagttatt ggtgccctta aacgcctggt gctacgcctg aataagtgat
961 aataagcgga tgaatggcag aaattcgccg gatctttgtg aaggaacctt acttctgtgg
1021 tgtgacataa ttggacaaac tacctacaga gatttaaagc tctaaggtaa atataaaatt
1081 tttaagtgta taatgtgtta aactactgat tctaattgtt tgtgtatttt agattccaac
1141 ctatggaact gatgaatggg agcagtggtg gaatgccttt aatgaggaaa acctgttttg
1201 ctcagaagaa atgccatcta gtgatgatga ggctactgct gactctcaac attctactcc
1261 tccaaaaaag aagagaaagg tagaagaccc caaggacttt ccttcagaat tgctaagttt
1321 tttgagtcat gctgtgttta gtaatagaac tcttgcttgc tttgctattt acaccacaaa
1381 ggaaaaagct gcactgctat acaagaaaat tatggaaaaa tattctgtaa cctttataag
1441 taggcataac agttataatc ataacatact gttttttctt actccacaca ggcatagagt
1501 gtctgctatt aataactatg ctcaaaaatt gtgtaccttt agctttttaa tttgtaaagg
1561 ggttaataag gaatatttga tgtatagtgc cttgactaga gatcataatc agccatacca
1621 catttgtaga ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac
1681 ataaaatgaa tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat
1741 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
1801 gtttgtccaa actcatcaat gtatcttatc atgtctggat cgatccccgg gtaccgagct
1861 cgaattcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
1921 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct
1981 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
2041 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt
2101 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
2161 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
2221 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
2281 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
2341 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
2401 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
2461 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
2521 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
2581 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
2641 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
2701 actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct
2761 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
2821 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
2881 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
2941 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
3001 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
3061 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
3121 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
3181 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
3241 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
3301 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
3361 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
3421 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
3481 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
3541 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
3601 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
3661 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
3721 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
3781 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
3841 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
3901 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
3961 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
4021 tgccacctga acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
4081 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
4141 aatgtatctt atcatgtctg gatcataatc agccatacca catttgtaga ggttttactt
4201 gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa tgcaattgtt
4261 gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
4321 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
4381 gtatcttatc atgtctggat ctcg
//