LOCUS LT727514 8800 bp DNA circular SYN 06-FEB-2017
DEFINITION Mammalian expression vector pEMBLyex4, complete sequence.
ACCESSION LT727514
VERSION LT727514.1
KEYWORDS .
SOURCE Mammalian expression vector pEMBLyex4
ORGANISM Mammalian expression vector pEMBLyex4
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 8800)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..8800
/organism="Mammalian expression vector pEMBLyex4"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 2423."
/db_xref="taxon:1945024"
rep_origin complement(754..1209)
/note="f1 ori"
CDS complement(1977..3071)
/transl_table=11
/note="defective LEU2"
/protein_id="SJL88706.1"
/translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENH
LIGGAAIDATGVPLPDEALEASKKVDAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQL
YANLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSE
QYTVPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLK
VQHQLIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPD
KNTAFGLYEPCHGSAPDLPKNKVDPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLD
AGIRTGDLGGSNSTTEVGDAVAEEVKKILA"
rep_origin complement(3429..4409)
/note="2mu ori"
misc_feature 4176..4778
/note="2mu inverted repeat"
regulatory complement(4491..4595)
/note="FLP terminator"
/regulatory_class="terminator"
regulatory complement(4909..5316)
/note="GAL10/CYC1 promoter"
/regulatory_class="promoter"
CDS complement(5535..6338)
/transl_table=11
/note="URA3"
/protein_id="SJL88707.1"
/translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKEL
LELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVK
LQYSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLS
TGEYTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYR
TVDDVVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
rep_origin complement(6793..7195)
/note="pMB1 ori"
CDS complement(7735..8595)
/transl_table=11
/note="ampicillin"
/protein_id="SJL88708.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
BASE COUNT 2494 a 1952 c 1893 g 2456 t
ORIGIN
1 ctgcctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac
61 ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc
121 gggtgttggc gggtgtcggg gcgcagccat gacccagtca cgtagcgata gcggagtnta
181 tactggctta actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt
241 gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggnaattcca acatccaata
301 aatcatacag gcaaggcaaa gaattagcaa aattaagcaa taaagcctca gagcataaag
361 ctaaatcggt tgtaccaaaa acattatgac cctgtaatac ttttgcggga gaagccttta
421 tttcaacgca aggataaaaa tttttagaac cctcatatat tttaaatgca atgcctgagt
481 aatgtgtagg taaagattca aacgggtgag aaaggccgga gacagtcaaa tcaccatcaa
541 tatgatattc aaccgttcta gctgataaat tcatgccgga gagggtagct atttttgaga
601 ggtctacaaa ggctatcagg tcattgcctg agagtctgga gcaaacaaga gaatcgatga
661 acggtaatcg taaaactagc atgtcaatca tatgtacccc ggttgataat cagaaaagcc
721 ccaaaaacag gaagattgta taagcaaata tttaaattgt aaacgttaat attttgttaa
781 aattcgcgtt aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca
841 aaatccctta taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga
901 acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc
961 agggcgatgg cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc
1021 gtaaagcact aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc
1081 cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg
1141 caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac
1201 agggcgcgta ctatggttgc tttgacgagc acgtataacg tgctttcctc gttagaatca
1261 gagcgggagc taaacaggag gccgattaaa gggattttag acaggaacgg tacgccagaa
1321 tcctgagaag tgtttttata atcagtgagg ccaccgagta aaagagtctg tccatcacgc
1381 aaattaaccg ttgtcgcaat acttctttga ttagtaataa catcacttgc ctgagtagaa
1441 gaactcaaac tatcggcctt gctggtaata tccagaacaa tattaccgcc agccattgca
1501 acaggaaaaa cgctcatgga aatacctaca ttttgacgct caatcgtctg gaattnccat
1561 tcgccattca ggctacgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta
1621 cgccanctgg cgaagggggg atgtgctgca aggcgattaa gttgggtaac gccagggttt
1681 tcccagtcag tacgttgtaa aacgacggcc agtgccaagc tccttaccac tcttttgtta
1741 ctctattgat ccagctcagc aaaggcagtg tgatctaaga ttctatcttc gcgatgtagt
1801 aaaactagct agaccgagaa agagactaga aatgcaaaag gcacttctac aatggctgcc
1861 atcattatta tccgatgtga cgctgcattt tttttttttt gtaatttcgt gtcgtttcta
1921 ttatgaattt catttataaa gtttatgtac aaatatcata aaaaaagaga atctttttaa
1981 gcaaggattt tcttaacttc ttcggcgaca gcatcaccga cttcggtggt actgttggaa
2041 ccacctaaat caccagttct gatacctgca tccaaaacct ttttaactgc atcttcaatg
2101 gccttacctt cttcaggcaa gttcaatgac aatttcaaca tcattgcagc agacaagata
2161 gtggcgatag ggtcaacctt attctttggc aaatctggag cagaaccatg gcatggttcg
2221 tacaaaccaa atgcggtgtt cttgtctggc aaagaggcca aggacgcaga tggcaacaaa
2281 cccaaggaac ctgggataac ggaggcttca tcggagatga tatcaccaaa catgttgctg
2341 gtgattataa taccatttag gtgggttggg ttcttaacta ggatcatggc ggcagaatca
2401 atcaattgat gttgaacctt caatgtaggg aattcgttct tgatggtttc ctccacagtt
2461 tttctccata atcttgaaga ggccaaaaca ttagctttat ccaaggacca aataggcaat
2521 ggtggctcat gttgtagggc catgaaagcg gccattcttg tgattctttg cacttctgga
2581 acggtgtatt gttcactatc ccaagcgaca ccatcaccat cgtcttcctt tctcttacca
2641 aagtaaatac ctcccactaa ttctctgaca acaacgaagt cagtaccttt agcaaattgt
2701 ggcttgattg gagataagtc taaaagagag tcggatgcaa agttacatgg tcttaagttg
2761 gcgtacaatt gaagttcttt acggattttt agtaaacctt gttcaggtct aacactaccg
2821 gtaccccatt taggaccacc cacagcacct aacaaaacgg catcaacctt cttggaggct
2881 tccagcgcct catctggaag tgggacacct gtagcatcga tagcagcacc accaattaaa
2941 tgattttcga aatcgaactt gacattggaa cgaacatcag aaatagcttt aagaacctta
3001 atggcttcgg ctgtgatttc ttgaccaacg tggtcacctg gcaaaacgac gatcttctta
3061 ggggcagaca ttacaatggt atatccttga aatatatata aaaaaaaaaa aaaatgcagc
3121 ttctcaatga tattcgaata cgctttgagg agatacagcc taatatccga caaactgttt
3181 tacagattta cgatcgtact tgttacccat cattgaattt tgaacatccg aacctgggag
3241 ttttccctga aacagatagt atatttgaac ctgtataata atatatagtc tagcgcttta
3301 cggaagacaa tgtatgtatt tcggttcctg gagaaactat tgcatctatt gcataggtaa
3361 tcttgcacgt cgcatccccg gttcattttc tgcgtttcca tcttgcactt caatagcata
3421 tctttgttaa cgaagcatct gtgcttcatt ttgtagaaca aaaatgcaac gcgagagcgc
3481 taatttttca aacaaagaat ctgagctgca tttttacaga acagaaatgc aacgcgaaag
3541 cgctatttta ccaacgaaga atctgtgctt catttttgta aaacaaaaat gcaacgcgag
3601 agcgctaatt tttcaaacaa agaatctgag ctgcattttt acagaacaga aatgcaacgc
3661 gagagcgcta ttttaccaac aaagaatcta tacttctttt ttgttctaca aaaatgcatc
3721 ccgagagcgc tatttttcta acaaagcatc ttagattact ttttttctcc tttgtgcgct
3781 ctataatgca gtctcttgat aactttttgc actgtaggtc cgttaaggtt agaagaaggc
3841 tactttggtg tctattttct cttccataaa aaaagcctga ctccacttcc cgcgtttact
3901 gattactagc gaagctgcgg gtgcattttt tcaagataaa ggcatccccg attatattct
3961 ataccgatgt ggattgcgca tactttgtga acagaaagtg atagcgttga tgattcttca
4021 ttggtcagaa aattatgaac ggtttcttct attttgtctc tatatactac gtataggaaa
4081 tgtttacatt ttcgtattgt tttcgattca ctctatgaat agttcttact acaatttttt
4141 tgtctaaaga gtaatactag agataaacat aaaaaatgta gaggtcgagt ttagatgcaa
4201 gttcaaggag cgaaaggtgg atgggtaggt tatataggga tatagcacag agatatatag
4261 caaagagata cttttgagca atgtttgtgg aagcggtatt cgcaatattt tagtagctcg
4321 ttacagtccg gtgcgttttt ggttttttga aagtgcgtct tcagagcgct tttggttttc
4381 aaaagcgctc tgaagttcct atactttcta gctagagaat aggaacttcg gaataggaac
4441 ttcaaagcgt ttccgaaaac gagcgcttcc gaaaatgcaa cgcgagctgc gcacatacag
4501 ctcactgttc acgtcgcacc tatatctgcg tgttgcctgt atatatatat acatgagaag
4561 aacggcatag tgcgtgttta tgcttaaatg cgtacttata tgcgtctatt tatgtaggat
4621 gaaaggtagt ctagtacctc ctgtgatatt atcccattcc atgcggggta tcgtatgctt
4681 ccttcagcac taccctttag ctgttctata tgctgccact cctcaattgg attagtctca
4741 tccttcaatg ctatcatttc ctttgatatt ggatcataag cttgcatgcc tgcaggtcga
4801 ctctagagga tccccgggta ccgagctcga attgatccgg taatttagtg tgtgtatttg
4861 tgtttgcgtg tctatagaag tatagtaatt tatgctacaa aggacctaat gtataaggaa
4921 agaatattta gagaaaagaa gaaaacaaga gttttatata catacagagc acatgcatgc
4981 catatgatca tgtgtcgtcg cacacatata tatatgcctg tatgtgtcag cactaaagtt
5041 gcctggccat ccacgctata tacacgcctg gcggatctgc tcgaggtcga ttagcctaaa
5101 aaaaccttct ctttggaact ttcagtaata cgcttaactg ctcattgcta tattgaagta
5161 cggattagaa gccgccgagc gggtgacagc cctccgaagg aagactctcc tccgtgcgtc
5221 ctcgtcttca ccggtcgcgt tcctgaaacg cagatgtgcc tcgcgccgca ctgctccgaa
5281 caataaagat tctacaatac tagcttttat ggttatgaag aggaaaaatt ggcagtaacc
5341 tggccccaca aaccttcaaa tgaacgaatc aaattaacaa ccataggatg ataatgcgat
5401 tagtttttta gccttatttc tggggtaatt aatcagcgaa gcgatgattt ttgatcgggt
5461 aataactgat ataattaaat tgaagctcta atttgtgagt ttagtataca tgcatttact
5521 tataatacag ttttttagtt ttgctggccg catcttctca aatatgcttc ccagcctgct
5581 tttctgtaac gttcaccctc taccttagca tcccttccct ttgcaaatag tcctcttcca
5641 acaataataa tgtcagatcc tgtagagacc acatcatcca cggttctata ctgttgaccc
5701 aatgcgtctc ccttgtcatc taaacccaca ccgggtgtca taatcaacca atcgtaacct
5761 tcatctcttc cacccatgtc tctttgagca ataaagccga taacaaaatc tttgtcgctc
5821 ttcgcaatgt caacagtacc cttagtatat tctccagtag atagggagcc cttgcatgac
5881 aattctgcta acatcaaaag gcctctaggt tcctttgtta cttcttctgc cgcctgcttc
5941 aaaccgctaa caatacctgg gcccaccaca ccgtgtgcat tcgtaatgtc tgcccattct
6001 gctattctgt atacacccgc agagtactgc aatttgactg tattaccaat gtcagcaaat
6061 tttctgtctt cgaagagtaa aaaattgtac ttggcggata atgcctttag cggcttaact
6121 gtgccctcca tggaaaaatc agtcaagata tccacatgtg tttttagtaa acaaattttg
6181 ggacctaatg cttcaactaa ctccagtaat tccttggtgg tacgaacatc caatgaagca
6241 cacaagtttg tttgcttttc gtgcatgata ttaaatagct tggcagcaac aggactagga
6301 tgagtagcag cacgttcctt atatgtagct ttcgacatga tttatcttcg tttccggttt
6361 ttgttctgtg cagttgggtt aagaatactg ggcaatttca tgtttcttca acactacata
6421 tgcgtatata taccaatcta agtctgtgct ccttccttcg ttcttccttc tgttcggaga
6481 ttaccgaatc aaaaaaattt caaagaaacc gaaatcaaaa aaaagaataa aaaaaaaatg
6541 atgaattgaa ttgaaaagct aattcgtaat catggtcata gctgtttcct gtgtgaaatt
6601 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg
6661 gtgcctaatg agtgaggtaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
6721 cgggaaacct gtcgtgccan ctggattaat gaatcggcca acgcgcgggg agaggcggtt
6781 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
6841 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
6901 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
6961 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
7021 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
7081 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
7141 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
7201 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
7261 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
7321 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
7381 tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc
7441 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
7501 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
7561 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
7621 gttagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
7681 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
7741 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
7801 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
7861 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
7921 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
7981 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
8041 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
8101 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
8161 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
8221 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
8281 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
8341 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
8401 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
8461 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
8521 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
8581 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
8641 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
8701 cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac
8761 ctataaaaat aggcgtatca cgaggccctt tcgtcttcaa
//