LOCUS LT727502 8424 bp DNA circular SYN 06-FEB-2017
DEFINITION Mammalian expression vector pSV55i2, complete sequence.
ACCESSION LT727502
VERSION LT727502.1
KEYWORDS .
SOURCE Mammalian expression vector pSV55i2
ORGANISM Mammalian expression vector pSV55i2
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 8424)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..8424
/organism="Mammalian expression vector pSV55i2"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 2338."
/db_xref="taxon:1945119"
regulatory 1..278
/note="SV40 early promoter"
/regulatory_class="promoter"
rep_origin 235..310
/note="SV40 ori"
misc_feature 349..594
/note="SV40 large T-antigen"
misc_feature 349..877
/note="SV40 small t-antigen"
misc_feature 595..944
/note="intron"
misc_feature 945..2825
/note="SV40 large T-antigen"
regulatory complement(2826..2922)
/note="polyA"
/regulatory_class="terminator"
regulatory 2826..2922
/note="polyA"
/regulatory_class="terminator"
misc_feature complement(3019..3956)
/note="intron"
misc_feature complement(3957..4159)
/note="5UTR"
regulatory complement(4192..4459)
/note="SV40 late promoter"
/regulatory_class="promoter"
regulatory 4215..4496
/note="SV40 early promoter"
/regulatory_class="promoter"
rep_origin 4453..4528
/note="SV40 ori"
misc_feature 4567..4812
/note="SV40 large T-antigen"
CDS 4567..5091
/transl_table=11
/note="SV40 small t-antigen"
/protein_id="SJL88673.1"
/translation="MDKVLNREESLQLMDLLGLERSAWGNIPLMRKAYLKKCKEFHPD
KGGDEEKMKKMNTLYKKMEDGVKYAHQPDFGGFWDATEVFASSLNPGVDAMYCKQWPE
CAKKMSANCICLLCLLRMKHENRKLYRKDPLVWVDCYCFDCFRMWFGLDLCEGTLLLW
CDIIGQTTYRDLKL"
misc_feature 4813..5158
/note="intron"
misc_feature 5159..6221
/note="part of SV40 large t-antigen"
rep_origin complement(6505..6905)
/note="pMB1 ori"
CDS complement(7448..8308)
/transl_table=11
/note="ampicillin resistance"
/protein_id="SJL88674.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
BASE COUNT 2504 a 1696 c 1906 g 2318 t
ORIGIN
1 ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
61 aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag
121 gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc
181 cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa
241 ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt
301 gaggaggctt ttttggaggc ctaggctttt gcaaaaagct ttgcaaagat ggataaagtt
361 ttaaacagag aggaatcttt gcagctaatg gaccttctag gtcttgaaag gagtgcctgg
421 gggaatattc ctctgatgag aaaggcatat ttaaaaaaat gcaaggagtt tcatcctgat
481 aaaggaggag atgaagaaaa aatgaagaaa atgaatactc tgtacaagaa aatggaagat
541 ggagtaaaat atgctcatca acctgacttt ggaggcttct gggatgcaac tgaggtattt
601 gcttcttcct taaatcctgg tgttgatgca atgtactgca aacaatggcc tgagtgtgca
661 aagaaaatgt ctgctaactg catatgcttg ctgtgcttac tgaggatgaa gcatgaaaat
721 agaaaattat acaggaaaga tccaccagat ctggtgggtt gattgctact gcttcgattg
781 ctttagaatg tggtttggac ttgatctttg tgaaggaacc ttacttctgt ggtgtgacat
841 aattggacaa actacctaca gagatttaaa gctctaaggt aaatataaaa tttttaagtg
901 tataatgtgt taaactactg attctaattg tttgtgtatt ttagattcca acctatggaa
961 ctgatgaatg ggagcagtgg tggaatgcct ttaatgagga aaacctgttt tgctcagaag
1021 aaatgccatc tagtgatgat gaggctactg ctgactctca acattctact cctccaaaaa
1081 agaagagaaa ggtagaagac cccaaggact ttccttcaga attgctaagt tttttgagtc
1141 atgctgtgtt tagtaataga actcttgctt gctttgctat ttacaccaca aaggaaaaag
1201 ctgcactgct atacaagaaa attatggaaa aatattctgt aacctttata agtaggcata
1261 acagttataa tcataacata ctgttttttc ttactccaca caggcataga gtgtctgcta
1321 ttaataacta tgctcaaaaa ttgtgtacct ttagcttttt aatttgtaaa ggggttaata
1381 aggaatattt gatgtatagt gccttgacta gagatccatt ttctgttatt gaggaaagtt
1441 tgccaggtgg gttaaaggag catgatttta atccagaaga agcagaggaa actaaacaag
1501 tgtcctggaa gcttgtaaca gagtatgcaa tggaaacaaa atgtgatgat gtgttgttat
1561 tgcttgggat gtacttggaa tttcagtaca gttttgaaat gtgtttaaaa tgtattaaaa
1621 aagaacagcc cagccactat aagtaccatg aaaagcatta tgcaaatgct gctatatttg
1681 ctgacagcaa aaaccaaaaa accatatgcc aacaggctgt tgatactgtt ttagctaaaa
1741 agcgggttga tagcctacaa ttaactagag aacaaatgtt aacaaacaga tttaatgatc
1801 ttttggatag gatggatata atgtttggtt ctacaggctc tgctgacata gaagaatgga
1861 tggctggagt tgcttggcta cactgtttgt tgcccaaaat ggattcagtg gtgtatgact
1921 ttttaaaatg catggtgtac aacattccta aaaaaagata ctggctgttt aaaggaccaa
1981 ttgatagtgg taaaactaca ttagcagctg ctttgcttga attatgtggg gggaaagctt
2041 taaatgttaa tttgcccttg gacaggctga actttgagct aggagtagct attgaccagt
2101 ttttagtagt ttttgaggat gtaaagggca ctggagggga gtccagagat ttgccttcag
2161 gtcagggaat taataacctg gacaatttaa gggattattt ggatggcagt gttaaggtaa
2221 acttagaaaa gaaacaccta aataaaagaa ctcaaatatt tccccctgga atagtcacca
2281 tgaatgagta cagtgtgcct aaaacactgc aggccagatt tgtaaaacaa atagatttta
2341 ggcccaaaga ttatttaaag cattgcctgg aacgcagtga gtttttgtta gaaaagagaa
2401 taattcaaag tggcattgct ttgcttctta tgttaatttg gtacagacct gtggctgagt
2461 ttgctcaaag tattcagagc agaattgtgg agtggaaaga gagattggac aaagagttta
2521 gtttgtcagt gtatcaaaaa atgaagttta atgtggctat gggaattgga gttttagatt
2581 ggctaagaaa cagtgatgat gatgatgaag acagccagga aaatgctgat aaaaatgaag
2641 atggtgggga gaagaacatg gaagactcag ggcatgaaac aggcattgat tcacagtccc
2701 aaggctcatt tcaggcccct cagtcctcac agtctgttca tgatcataat cagccatacc
2761 acatttgtag aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa
2821 cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa
2881 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
2941 ggtttgtcca aactcatcaa tgtatcttat catgtctgga tccggagctt ttagagcaga
3001 agtaacactt ccgtacaggc ctagaagtaa aggcaacatc cactgaggag cagttctttg
3061 atttgcacca ccaggagcct caaatttttc aataaattca cctgactgca cattaggact
3121 ttggctttga gcttcccacc tctcagttac ttgctgaata ctgtctgctt catcaatatt
3181 atcataggtg tgcccaaatg atatttgcaa cccttccctg ttggctactt gtctcaccat
3241 tgtaggccta atgggagaca aagtagagta gtaatcttgt aaagagttat accaattaac
3301 aggagcatta attactgtcc aagtagtttc ctctaaaaac cttgccaaac tgtcccttaa
3361 atatctttgg gttcttcttt caagctcctg tgaggtgagc ctaggaatgt cattttgtat
3421 tacacgccaa aaagcttgag aaatggcatt aaaaagtgtt ggaccccaat gtctggggtc
3481 aagatactga acactgtgaa caaaggtttg tactccagga aataaaatat catagtaatc
3541 atctggccta tacaaatcta cagccattcc tggttgttga tataaaccaa cagtagaaac
3601 tttgtgatcc cagtcactaa aaaatctata ccccacttga gcaacagcgc tcacaccagt
3661 cacagtttgc agtaaagctg caaatccagc tatagcagca ggagccccag atatcacagc
3721 ataggcctgt ggagtgaggc ctatagcagc aattgcctca gaggttgtta ggccttcaac
3781 agtagcaaca gatgcaagtt gcacttcaat tgcagcagcg gcctctccag cagcaatttc
3841 agctactgaa aatccagtag cagcagcagc ttcagacaca gtagcaatta ggtcccccaa
3901 cagtgttaaa gcagcaccca tggacctgaa ataaaagaca aaaagactaa acttaccagt
3961 taactttctg gtttttcagt taacctttct ggttttttgc gtttcccgtc aacagtatct
4021 tccccttcac aaaattgcag caaaagctct aaaacaaaca caaaaaggcg ttgagctgtt
4081 tttttacttt cagtccatga cctacgaacc ttaacggagg cctggcgtga cagccggcgc
4141 agcaccatgg cctgaaataa cctctgaaag aggaacttgg ttagggaatt cccttctgag
4201 gcggaaagaa ccagctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc
4261 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt
4321 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
4381 tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc
4441 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg
4501 agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagcttt
4561 gcaaagatgg ataaagtttt aaacagagag gaatctttgc agctaatgga ccttctaggt
4621 cttgaaagga gtgcctgggg gaatattcct ctgatgagaa aggcatattt aaaaaaatgc
4681 aaggagtttc atcctgataa aggaggagat gaagaaaaaa tgaagaaaat gaatactctg
4741 tacaagaaaa tggaagatgg agtaaaatat gctcatcaac ctgactttgg aggcttctgg
4801 gatgcaactg aggtatttgc ttcttcctta aatcctggtg ttgatgcaat gtactgcaaa
4861 caatggcctg agtgtgcaaa gaaaatgtct gctaactgca tatgcttgct gtgcttactg
4921 aggatgaagc atgaaaatag aaaattatac aggaaagatc cacttgtgtg ggttgattgc
4981 tactgcttcg attgctttag aatgtggttt ggacttgatc tttgtgaagg aaccttactt
5041 ctgtggtgtg acataattgg acaaactacc tacagagatt taaagctcta aggtaaatat
5101 aaaattttta agtgtataat gtgttaaact actgattcta attgtttgtg tattttagat
5161 tccaacctat ggaactgatg aatgggagca gtggtggaat gcctttaatg aggaaaacct
5221 gttttgctca gaagaaatgc catctagtga tgatgaggct actgctgact ctcaacattc
5281 tactcctcca aaaaagaaga gaaaggtaga agaccccaag gactttcctt cagaattgct
5341 aagttttttg agtcatgctg tgtttagtaa tagaactctt gcttgctttg ctatttacac
5401 cacaaaggaa aaagctgcac tgctatacaa gaaaattatg gaaaaatatt ctgtaacctt
5461 tataagtagg cataacagtt ataatcataa catactgttt tttcttactc cacacaggca
5521 tagagtgtct gctattaata actatgctca aaaattgtgt acctttagct ttttaatttg
5581 taaaggggtt aataaggaat atttgatgta tagtgccttg actagagatc cattttctgt
5641 tattgaggaa agtttgccag gtgggttaaa ggagcatgat tttaatccag aagaagcaga
5701 ggaaactaaa caagtgtcct ggaagcttgt aacagagtat gcaatggaaa caaaatgtga
5761 tgatgtgttg ttattgcttg ggatgtactt ggaatttcag tacagttttg aaatgtgttt
5821 aaaatgtatt aaaaaagaac agcccagcca ctataagtac catgaaaagc attatgcaaa
5881 tgctgctata tttgctgaca gcaaaaacca aaaaaccata tgccaacagg ctgttgatac
5941 tgttttagct aaaaagcggg ttgatagcct acaattaact agagaacaaa tgttaacaaa
6001 cagatttaat gatcttttgg ataggatgga tataatgttt ggttctacag gctctgctga
6061 catagaagaa tggatggctg gagttgcttg gctacactgt ttgttgccca aaatggattc
6121 agtggtgtat gactttttaa aatgcatggt gtacaacatt cctaaaaaaa gatactggct
6181 gtttaaagga ccaattgata gtggtaaaac tacattagca gctgcctcgc gcgtttcggt
6241 gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa
6301 gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg
6361 ggcgcagcca tgacccagtc acgtagcgat agcggagtgt atactggctt aactatgcgg
6421 catcagagca gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg
6481 taaggagaaa ataccgcatc aggcgctctt ccgcttcctc gctcactgac tcgctgcgct
6541 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
6601 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
6661 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
6721 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
6781 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
6841 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
6901 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
6961 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
7021 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
7081 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg
7141 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
7201 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
7261 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
7321 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
7381 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
7441 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
7501 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
7561 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
7621 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
7681 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
7741 tgcgcaacgt tgttgccatt gctgcaggca tcgtggtgtc acgctcgtcg tttggtatgg
7801 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
7861 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
7921 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
7981 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
8041 cgagttgctc ttgcccggcg tcaacacggg ataataccgc gccacatagc agaactttaa
8101 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
8161 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
8221 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
8281 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
8341 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
8401 taggggttcc gcgcacattt cccc
//