LOCUS LT727496 7639 bp DNA circular SYN 06-FEB-2017
DEFINITION Vector pMaSVTHIL61, complete sequence.
ACCESSION LT727496
VERSION LT727496.1
KEYWORDS .
SOURCE Vector pMaSVTHIL61
ORGANISM Vector pMaSVTHIL61
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 7639)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..7639
/organism="Vector pMaSVTHIL61"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 2331."
/db_xref="taxon:1945342"
regulatory 24..105
/note="fdT"
/regulatory_class="terminator"
regulatory 134..215
/note="fdT"
/regulatory_class="terminator"
rep_origin 313..768
/note="f1 ori"
rep_origin complement(772..1145)
/note="pMB1 ori"
CDS complement(1686..2546)
/transl_table=11
/note="ampicillin resistance"
/protein_id="SJL88653.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
misc_feature complement(2861..3520)
/note="chloramphenicol sensitive"
misc_feature 3777..4413
/note="5FR"
regulatory 4414..4949
/note="hIL6"
/regulatory_class="promoter"
misc_feature 4994..5239
/note="SV40 large T-antigen"
CDS 4994..5518
/transl_table=11
/note="SV40 small t-antigen"
/protein_id="SJL88654.1"
/translation="MDKVLNREESLQLMDLLGLERSAWGNIPLMRKAYLKKCKEFHPD
KGGDEEKMKKMNTLYKKMEDGVKYAHQPDFGGFWDATEVFASSLNPGVDAMYCKQWPE
CAKKMSANCICLLCLLRMKHENRKLYRKDPLVWVDCYCFDCFRMWFGLDLCEGTLLLW
CDIIGQTTYRDLKL"
misc_feature 5240..5585
/note="intron"
misc_feature 5586..7466
/note="SV40 large T-antigen"
regulatory 7467..7563
/note="polyA"
/regulatory_class="terminator"
regulatory complement(7467..7563)
/note="polyA"
/regulatory_class="terminator"
BASE COUNT 2193 a 1618 c 1733 g 2095 t
ORIGIN
1 aattcacctc gaaagcaagc tgataaaccg atacaattaa aggctccttt tggagccttt
61 ttttttggag attttcaacg tgaaaaaatt attattcgca attccaagct aattcacctc
121 gaaagcaagc tgataaaccg atacaattaa aggctccttt tggagccttt ttttttggag
181 attttcaacg tgaaaaaatt attattcgca attccaagct ctgcctcgcg cgtttcggtg
241 atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag
301 cggatgcaga tcacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc
361 gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt
421 cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag
481 ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt
541 cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt
601 tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt
661 cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt
721 aacaaaaatt taacgcgaat tttaacaaaa tattaacgtt tacaatttga tctgcgctcg
781 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
841 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
901 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
961 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
1021 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
1081 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
1141 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
1201 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
1261 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
1321 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt
1381 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
1441 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
1501 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
1561 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
1621 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
1681 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
1741 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
1801 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
1861 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
1921 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
1981 cgcaacgttg ttgccattgc tgcaggcatc gtggtgtcac gctcgtcgtt tggtatggct
2041 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
2101 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
2161 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
2221 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
2281 agttgctctt gcccggcgtc aacacgggat aataccgcgc cacatagcag aactttaaaa
2341 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
2401 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
2461 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
2521 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcagacag
2581 ttttattgtt catgatgata tatttttatc ttgtgcaatg taacatcaga gattttgaga
2641 cacaacgtgg ctttgttgaa taaatcgaac ttttgctgag ttgactcccc gcgcgcgatg
2701 ggtcgaattt gctttcgaaa aaaaagcccg ctcattaggc gggctaaaaa aaagcccgct
2761 cattaggcgg gctcgaattt ctgccattca tccgcttatt atcacttatt caggcgtagc
2821 aaccaggcgt ttaagggcac caataactgc cttaaaaaaa ttacgccccg ccctgccact
2881 catcgcagta ctgttgtaat tcattaagca ttctgccgac atggaagcca tcacagacgg
2941 catgatgaac ctgaatcgcc agcggcatca gcaccttgtc gccttgcgta taatatttgc
3001 ccatagtgaa aacgggggcg aagaagttgt ccatattcgc cacgtttaaa tcaaaactgg
3061 tgaaactcac ccagggattg gctgagacga aaaacatatt ctcaataaac cctttaggga
3121 aataggccag gttttcaccg taacacgcca catcttgcga atatatgtgt agaaactgcc
3181 ggaaatcgtc gtggtattca ctccagagcg atgaaaacgt ttcagtttgc tcatggaaaa
3241 cggtgtaaca agggtgaaca ctatcccata tcaccagctc accgtctttc attgccatac
3301 gaaattccgg atgagcattc atcaggcggg caagaatgtg aataaaggcc ggataaaact
3361 tgtgcttatt tttctttacg gtctttaaaa aggccgtaat atccagctaa acggtctggt
3421 tataggtaca ttgagcaact gactgaaatg cctcaaaatg ttctttacga tgccattggg
3481 atatatcaac ggtggtatat ccagtgattt ttttctccat tttagcttcc ttagctcctg
3541 aaaatctcga taactcaaaa aatacgcccg gtagtgatct tatttcatta tggtgaaagt
3601 tggaacctct tacgtgccga tcaacgtctc attttcgcca aaagttggcc cagggcttcc
3661 cggtatcaac agggacacca ggatttattt attctgcgaa gtgatcttcc gtcacaggta
3721 tttattcgaa gacgaaaggg catcgcgcgc ggggaattcg agctcgcccg gggatcctcc
3781 tgcaagagac accatcctga gggaagaggg cttctgaacc agcttgaccc aataagaaat
3841 tcttgggtgc cgacgcggaa gcagattcag agcctagagc cgtgcctgcg tccgtagttt
3901 ccttctagct tcttttgatt tcaaatcaag acttacaggg agagggagcg ataaacacaa
3961 actctgcaag atgccacaag gtcctccttt gacatcccca acaaagaggt gagtagtatt
4021 ctcccccttt ctgccctgaa ccaagtgggc ttcagtaatt tcagggctcc aggagacctg
4081 gggcccatgc aggtgcccca gtgaaacagt ggtgaagaga ctcagtggca atggggagag
4141 cactggcagc acaaggcaaa cctctggcac agagagcaaa gtcctcactg ggaggattcc
4201 caaggggtca cttgggagag ggcagggcag cagccaacct cctctaagtg ggctgaagca
4261 ggtgaagaaa gtggcagaag ccacgcggtg gcaaaaagga gtcacacact ccacctggag
4321 acgccttgaa gtaactgcac gaaatttgag gatggccagg cagttctaca acagccgctc
4381 acagggagag ccagaacaca gaagaactca gatgactggt agtattacct tcttcataat
4441 cccaggcttg gggggctgcg atggagtcag aggaaactca gttcagaaca tctttggttt
4501 ttacaaatac aaattaactg gaacgctaaa ttctagcctg ttaatctggt cactgaaaaa
4561 aaattttttt tttttcaaaa aacatagctt tagcttattt tttttctctt tgtaaaactt
4621 cgtgcatgac ttcagcttta ctctttgtca agacatgcca aagtgctgag tcactaataa
4681 aagaaaaaaa gaaagtaaag gaagagtggt tctgcttctt agcgctagcc tcaatgacga
4741 cctaagctgc acttttcccc ctagttgtgt cttgcgatgc taaaggacgt cacattgcac
4801 aatcttaata aggtttccaa tcagccccac ccgctctggc cccaccctca ccctccaaca
4861 aagatttatc aaatgtggga ttttcccatg agtctcaata ttagagtctc aacccccaat
4921 aaatatagga ctggagatgt ctgaggctca ttctgccctc gaggcctagg cttttgcaaa
4981 aagctttgca aagatggata aagttttaaa cagagaggaa tctttgcagc taatggacct
5041 tctaggtctt gaaaggagtg cctgggggaa tattcctctg atgagaaagg catatttaaa
5101 aaaatgcaag gagtttcatc ctgataaagg aggagatgaa gaaaaaatga agaaaatgaa
5161 tactctgtac aagaaaatgg aagatggagt aaaatatgct catcaacctg actttggagg
5221 cttctgggat gcaactgagg tatttgcttc ttccttaaat cctggtgttg atgcaatgta
5281 ctgcaaacaa tggcctgagt gtgcaaagaa aatgtctgct aactgcatat gcttgctgtg
5341 cttactgagg atgaagcatg aaaatagaaa attatacagg aaagatccac ttgtgtgggt
5401 tgattgctac tgcttcgatt gctttagaat gtggtttgga cttgatcttt gtgaaggaac
5461 cttacttctg tggtgtgaca taattggaca aactacctac agagatttaa agctctaagg
5521 taaatataaa atttttaagt gtataatgtg ttaaactact gattctaatt gtttgtgtat
5581 tttagattcc aacctatgga actgatgaat gggagcagtg gtggaatgcc tttaatgagg
5641 aaaacctgtt ttgctcagaa gaaatgccat ctagtgatga tgaggctact gctgactctc
5701 aacattctac tcctccaaaa aagaagagaa aggtagaaga ccccaaggac tttccttcag
5761 aattgctaag ttttttgagt catgctgtgt ttagtaatag aactcttgct tgctttgcta
5821 tttacaccac aaaggaaaaa gctgcactgc tatacaagaa aattatggaa aaatattctg
5881 taacctttat aagtaggcat aacagttata atcataacat actgtttttt cttactccac
5941 acaggcatag agtgtctgct attaataact atgctcaaaa attgtgtacc tttagctttt
6001 taatttgtaa aggggttaat aaggaatatt tgatgtatag tgccttgact agagatccat
6061 tttctgttat tgaggaaagt ttgccaggtg ggttaaagga gcatgatttt aatccagaag
6121 aagcagagga aactaaacaa gtgtcctgga agcttgtaac agagtatgca atggaaacaa
6181 aatgtgatga tgtgttgtta ttgcttggga tgtacttgga atttcagtac agttttgaaa
6241 tgtgtttaaa atgtattaaa aaagaacagc ccagccacta taagtaccat gaaaagcatt
6301 atgcaaatgc tgctatattt gctgacagca aaaaccaaaa aaccatatgc caacaggctg
6361 ttgatactgt tttagctaaa aagcgggttg atagcctaca attaactaga gaacaaatgt
6421 taacaaacag atttaatgat cttttggata ggatggatat aatgtttggt tctacaggct
6481 ctgctgacat agaagaatgg atggctggag ttgcttggct acactgtttg ttgcccaaaa
6541 tggattcagt ggtgtatgac tttttaaaat gcatggtgta caacattcct aaaaaaagat
6601 actggctgtt taaaggacca attgatagtg gtaaaactac attagcagct gctttgcttg
6661 aattatgtgg ggggaaagct ttaaatgtta atttgccctt ggacaggctg aactttgagc
6721 taggagtagc tattgaccag tttttagtag tttttgagga tgtaaagggc actggagggg
6781 agtccagaga tttgccttca ggtcagggaa ttaataacct ggacaattta agggattatt
6841 tggatggcag tgttaaggta aacttagaaa agaaacacct aaataaaaga actcaaatat
6901 ttccccctgg aatagtcacc atgaatgagt acagtgtgcc taaaacactg caggccagat
6961 ttgtaaaaca aatagatttt aggcccaaag attatttaaa gcattgcctg gaacgcagtg
7021 agtttttgtt agaaaagaga ataattcaaa gtggcattgc tttgcttctt atgttaattt
7081 ggtacagacc tgtggctgag tttgctcaaa gtattcagag cagaattgtg gagtggaaag
7141 agagattgga caaagagttt agtttgtcag tgtatcaaaa aatgaagttt aatgtggcta
7201 tgggaattgg agttttagat tggctaagaa acagtgatga tgatgatgaa gacagccagg
7261 aaaatgctga taaaaatgaa gatggtgggg agaagaacat ggaagactca gggcatgaaa
7321 caggcattga ttcacagtcc caaggctcat ttcaggcccc tcagtcctca cagtctgttc
7381 atgatcataa tcagccatac cacatttgta gaggttttac ttgctttaaa aaacctccca
7441 cacctccccc tgaacctgaa acataaaatg aatgcaattg ttgttgttaa cttgtttatt
7501 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
7561 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg
7621 atccggctct agaggtcga
//