LOCUS LT727494 4381 bp DNA circular SYN 06-FEB-2017
DEFINITION Vector pGEMIL6gIL2f2, complete sequence.
ACCESSION LT727494
VERSION LT727494.1
KEYWORDS .
SOURCE Vector pGEMIL6gIL2f2
ORGANISM Vector pGEMIL6gIL2f2
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 4381)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..4381
/organism="Vector pGEMIL6gIL2f2"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 2319."
/db_xref="taxon:1945306"
CDS complement(<23..>113)
/codon_start=1
/transl_table=11
/note="hIL2"
/protein_id="SJL88648.1"
/translation="NYKNPKLTRMLTFKFYMPKKATELKHLQCL"
misc_feature complement(114..203)
/note="intron"
CDS complement(<204..350)
/transl_table=11
/note="hIL2"
/protein_id="SJL88649.1"
/translation="MYRMQLLSCIALSLALVTNSAPTSSSTKKTQLQLEHLLLDLQMI
LNGIN"
regulatory complement(372..907)
/note="hIL6"
/regulatory_class="promoter"
misc_feature complement(908..1544)
/note="5FR"
regulatory complement(1577..1593)
/note="T7"
/regulatory_class="promoter"
rep_origin complement(1661..2063)
/note="pMB1 ori"
CDS complement(2604..3464)
/transl_table=11
/note="ampicillin resistance"
/protein_id="SJL88650.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
regulatory 4359..4375
/note="SP6"
/regulatory_class="promoter"
BASE COUNT 1091 a 1066 c 1085 g 1139 t
ORIGIN
1 aagcttgggc tgcaggtcga ctctagacac tgaagatgtt tcagttctgt ggccttcttg
61 ggcatgtaaa acttaaatgt gagcatcctg gtgagtttgg gattcttgta attctaagaa
121 agtataatgc attgttatta agaaatgatc tccagctaga ttactaaatg taataatttt
181 agtaagaaag gaaatatact tacattaatt ccattcaaaa tcatctgtaa atccagcagt
241 aaatgctcca gttgtagctg tgttttcttt gtagaacttg aagtaggtgc actgtttgtg
301 acaagtgcaa gacttagtgc aatgcaagac aggagttgca tcctgtacat tgtggccctc
361 gagggcagaa tgagcctcag acatctccag tcctatattt attgggggtt gagactctaa
421 tattgagact catgggaaaa tcccacattt gataaatctt tgttggaggg tgagggtggg
481 gccagagcgg gtggggctga ttggaaacct tattaagatt gtgcaatgtg acgtccttta
541 gcatcgcaag acacaactag ggggaaaagt gcagcttagg tcgtcattga ggctagcgct
601 aagaagcaga accactcttc ctttactttc tttttttctt ttattagtga ctcagcactt
661 tggcatgtct tgacaaagag taaagctgaa gtcatgcacg aagttttaca aagagaaaaa
721 aaataagcta aagctatgtt ttttgaaaaa aaaaaaattt tttttcagtg accagattaa
781 caggctagaa tttagcgttc cagttaattt gtatttgtaa aaaccaaaga tgttctgaac
841 tgagtttcct ctgactccat cgcagccccc caagcctggg attatgaaga aggtaatact
901 accagtcatc tgagttcttc tgtgttctgg ctctccctgt gagcggctgt tgtagaactg
961 cctggccatc ctcaaatttc gtgcagttac ttcaaggcgt ctccaggtgg agtgtgtgac
1021 tcctttttgc caccgcgtgg cttctgccac tttcttcacc tgcttcagcc cacttagagg
1081 aggttggctg ctgccctgcc ctctcccaag tgaccccttg ggaatcctcc cagtgaggac
1141 tttgctctct gtgccagagg tttgccttgt gctgccagtg ctctccccat tgccactgag
1201 tctcttcacc actgtttcac tggggcacct gcatgggccc caggtctcct ggagccctga
1261 aattactgaa gcccacttgg ttcagggcag aaagggggag aatactactc acctctttgt
1321 tggggatgtc aaaggaggac cttgtggcat cttgcagagt ttgtgtttat cgctccctct
1381 ccctgtaagt cttgatttga aatcaaaaga agctagaagg aaactacgga cgcaggcacg
1441 gctctaggct ctgaatctgc ttccgcgtcg gcacccaaga atttcttatt gggtcaagct
1501 ggttcagaag ccctcttccc tcaggatggt gtctcttgca ggaggatccc cgggcgagct
1561 cgaattccgg tctccctata gtgagtcgta ttaatttcga taagccagct gcattaatga
1621 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
1681 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
1741 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
1801 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
1861 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
1921 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
1981 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
2041 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
2101 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
2161 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
2221 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
2281 agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
2341 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
2401 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
2461 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
2521 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
2581 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
2641 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
2701 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
2761 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
2821 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
2881 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
2941 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
3001 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
3061 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
3121 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
3181 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
3241 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
3301 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
3361 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
3421 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
3481 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
3541 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc
3601 taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt
3661 cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg
3721 gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg
3781 ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat cagagcagat tgtactgaga
3841 gtgcaccata tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta
3901 ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca
3961 acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc
4021 cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg
4081 cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc tggctagcga
4141 tgaccctgct gattggttcg ctgaccattt ccggggtgcg gaacggcgtt accagaaact
4201 cagaaggttc gtccaaccaa accgactctg acggcagttt acgagagaga tgatagggtc
4261 tgcttcagta agccagatgc tacacaatta ggcttgtaca tattgtcgtt agaacgcggc
4321 tacaattaat acataacctt atgtatcata cacatacgat ttaggtgaca ctatagaata
4381 c
//