LOCUS LT727489 5677 bp DNA circular SYN 06-FEB-2017
DEFINITION Bacterial expression vector pET-11a, complete sequence.
ACCESSION LT727489
VERSION LT727489.1
KEYWORDS .
SOURCE Bacterial expression vector pET-11a
ORGANISM Bacterial expression vector pET-11a
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 5677)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..5677
/organism="Bacterial expression vector pET-11a"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 2290."
/db_xref="taxon:1944663"
regulatory complement(213..260)
/note="T7 terminator"
/regulatory_class="terminator"
CDS complement(<328..360)
/transl_table=11
/note="T7 gene 10 fragment"
/protein_id="SJL88633.1"
/translation="MASMTGGQQMG"
misc_feature complement(361..405)
/note="5' UTR of T7 gene 10, incl. RBS"
regulatory complement(407..448)
/note="T7 promoter with lac operator"
/regulatory_class="promoter"
regulatory 757..834
/note="lacI promoter"
/regulatory_class="promoter"
CDS 835..1917
/transl_table=11
/note="lacI"
/protein_id="SJL88634.1"
/translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAE
LNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERS
GVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSII
FSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREG
DWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDT
EDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNT
QTASPRALADSLMQLARQVSRLESGQ"
CDS 3229..3420
/transl_table=11
/note="rop"
/protein_id="SJL88635.1"
/translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDH
ADELYRSCLARFGDDGENL"
rep_origin complement(3666..4068)
/note="pMB1 ori"
CDS complement(4609..5469)
/transl_table=11
/note="ampicillin resistance"
/protein_id="SJL88636.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
BASE COUNT 1299 a 1550 c 1496 g 1332 t
ORIGIN
1 ttctcatgtt tgacagctta tcatcgataa gctttaatgc ggtagtttat cacagttaaa
61 ttgctaacgc agtcaggcac cgtgtatgaa atctaacaat gcgctcatcg tcatcctcgg
121 caccgtcacc ctggatgctg taggcatagg cttggttatg ccggtactgc cgggcctctt
181 gcgggatatc cggatatagt tcctcctttc agcaaaaaac ccctcaagac ccgtttagag
241 gccccaaggg gttatgctag ttattgctca gcggtggcag cagccaactc agcttccttt
301 cgggctttgt tagcagccgg atccgcgacc catttgctgt ccaccagtca tgctagccat
361 atgtatatct ccttcttaaa gttaaacaaa attatttcta gaggggaatt gttatccgct
421 cacaattccc ctatagtgag tcgtattaat ttcgcgggat cgagatctcg atcctctacg
481 ccggacgcat cgtggccggc atcaccggcg ccacaggtgc ggttgctggc gcctatatcg
541 ccgacatcac cgatggggaa gatcgggctc gccacttcgg gctcatgagc gcttgtttcg
601 gcgtgggtat ggtggcaggc cccgtggccg ggggactgtt gggcgccatc tccttgcatg
661 caccattcct tgcggcggcg gtgctcaacg gcctcaacct actactgggc tgcttcctaa
721 tgcaggagtc gcataaggga gagcgtcgag atcccggaca ccatcgaatg gcgcaaaacc
781 tttcgcggta tggcatgata gcgcccggaa gagagtcaat tcagggtggt gaatgtgaaa
841 ccagtaacgt tatacgatgt cgcagagtat gccggtgtct cttatcagac cgtttcccgc
901 gtggtgaacc aggccagcca cgtttctgcg aaaacgcggg aaaaagtgga agcggcgatg
961 gcggagctga attacattcc caaccgcgtg gcacaacaac tggcgggcaa acagtcgttg
1021 ctgattggcg ttgccacctc cagtctggcc ctgcacgcgc cgtcgcaaat tgtcgcggcg
1081 attaaatctc gcgccgatca actgggtgcc agcgtggtgg tgtcgatggt agaacgaagc
1141 ggcgtcgaag cctgtaaagc ggcggtgcac aatcttctcg cgcaacgcgt cagtgggctg
1201 atcattaact atccgctgga tgaccaggat gccattgctg tggaagctgc ctgcactaat
1261 gttccggcgt tatttcttga tgtctctgac cagacaccca tcaacagtat tattttctcc
1321 catgaagacg gtacgcgact gggcgtggag catctggtcg cattgggtca ccagcaaatc
1381 gcgctgttag cgggcccatt aagttctgtc tcggcgcgtc tgcgtctggc tggctggcat
1441 aaatatctca ctcgcaatca aattcagccg atagcggaac gggaaggcga ctggagtgcc
1501 atgtccggtt ttcaacaaac catgcaaatg ctgaatgagg gcatcgttcc cactgcgatg
1561 ctggttgcca acgatcagat ggcgctgggc gcaatgcgcg ccattaccga gtccgggctg
1621 cgcgttggtg cggatatctc ggtagtggga tacgacgata ccgaagacag ctcatgttat
1681 atcccgccgt taaccaccat caaacaggat tttcgcctgc tggggcaaac cagcgtggac
1741 cgcttgctgc aactctctca gggccaggcg gtgaagggca atcagctgtt gcccgtctca
1801 ctggtgaaaa gaaaaaccac cctggcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
1861 gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg
1921 caacgcaatt aatgtaagtt agctcactca ttaggcaccg ggatctcgac cgatgccctt
1981 gagagccttc aacccagtca gctccttccg gtgggcgcgg ggcatgacta tcgtcgccgc
2041 acttatgact gtcttcttta tcatgcaact cgtaggacag gtgccggcag cgctctgggt
2101 cattttcggc gaggaccgct ttcgctggag cgcgacgatg atcggcctgt cgcttgcggt
2161 attcggaatc ttgcacgccc tcgctcaagc cttcgtcact ggtcccgcca ccaaacgttt
2221 cggcgagaag caggccatta tcgccggcat ggcggccgac gcgctgggct acgtcttgct
2281 ggcgttcgcg acgcgaggct ggatggcctt ccccattatg attcttctcg cttccggcgg
2341 catcgggatg cccgcgttgc aggccatgct gtccaggcag gtagatgacg accatcaggg
2401 acagcttcaa ggatcgctcg cggctcttac cagcctaact tcgatcactg gaccgctgat
2461 cgtcacggcg atttatgccg cctcggcgag cacatggaac gggttggcat ggattgtagg
2521 cgccgcccta taccttgtct gcctccccgc gttgcgtcgc ggtgcatgga gccgggccac
2581 ctcgacctga atggaagccg gcggcacctc gctaacggat tcaccactcc aagaattgga
2641 gccaatcaat tcttgcggag aactgtgaat gcgcaaacca acccttggca gaacatatcc
2701 atcgcgtccg ccatctccag cagccgcacg cggcgcatct cgggcagcgt tgggtcctgg
2761 ccacgggtgc gcatgatcgt gctcctgtcg ttgaggaccc ggctaggctg gcggggttgc
2821 cttactggtt agcagaatga atcaccgata cgcgagcgaa cgtgaagcga ctgctgctgc
2881 aaaacgtctg cgacctgagc aacaacatga atggtcttcg gtttccgtgt ttcgtaaagt
2941 ctggaaacgc ggaagtcagc gccctgcacc attatgttcc ggatctgcat cgcaggatgc
3001 tgctggctac cctgtggaac acctacatct gtattaacga agcgctggca ttgaccctga
3061 gtgatttttc tctggtcccg ccgcatccat accgccagtt gtttaccctc acaacgttcc
3121 agtaaccggg catgttcatc atcagtaacc cgtatcgtga gcatcctctc tcgtttcatc
3181 ggtatcatta cccccatgaa cagaaatccc ccttacacgg aggcatcagt gaccaaacag
3241 gaaaaaaccg cccttaacat ggcccgcttt atcagaagcc agacattaac gcttctggag
3301 aaactcaacg agctggacgc ggatgaacag gcagacatct gtgaatcgct tcacgaccac
3361 gctgatgagc tttaccgcag ctgcctcgcg cgtttcggtg atgacggtga aaacctctga
3421 cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa
3481 gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gcgcagccat gacccagtca
3541 cgtagcgata gcggagtgta tactggctta actatgcggc atcagagcag attgtactga
3601 gagtgcacca tatatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat
3661 caggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
3721 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
3781 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
3841 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
3901 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
3961 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
4021 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
4081 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
4141 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
4201 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
4261 gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa
4321 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
4381 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
4441 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
4501 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
4561 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
4621 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
4681 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
4741 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
4801 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
4861 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
4921 tgctgcaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
4981 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
5041 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
5101 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
5161 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
5221 gtcaacacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
5281 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
5341 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
5401 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
5461 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
5521 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
5581 tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa
5641 aaataggcgt atcacgaggc cctttcgtct tcaagaa
//