LOCUS LT727482 4995 bp DNA circular SYN 06-FEB-2017
DEFINITION Mammalian expression vector pSV23SmIL6mTNF, complete sequence.
ACCESSION LT727482
VERSION LT727482.1
KEYWORDS .
SOURCE Mammalian expression vector pSV23SmIL6mTNF
ORGANISM Mammalian expression vector pSV23SmIL6mTNF
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 4995)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..4995
/organism="Mammalian expression vector pSV23SmIL6mTNF"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 3409."
/db_xref="taxon:1945077"
CDS <1..809
/codon_start=3
/transl_table=11
/note="part of SV40 VP1"
/protein_id="SJL88605.1"
/translation="PLPNLNEDLTCGNILMWEAVTVKTEVIGVTAMLNLHSGTQKTHE
NGAGKPIQGSNFHFFAVGGEPLELQGVLANYRTKYPAQTVTPKNATVDSQQMNTDHKA
VLDKDNAYPVECWVPDPSKNENTRYFGTYTGGENVPPVLHITNTATTVLLDEQGVGPL
CKADSLYVSAVDICGLFTNTSGTQQWKGLPRYFKITLRKRSVKNPYPISFLLSDLINR
RTQRVDGQPMIGMSSQVEEVRVYEDTEELPGDPDMIRYIDEFGQTTTRMQ"
regulatory complement(810..906)
/note="SV40 polyA early"
/regulatory_class="terminator"
regulatory 810..906
/note="SV40 polyA late"
/regulatory_class="terminator"
misc_feature complement(907..1458)
/note="3' UTR of SV40 small t-antigen"
misc_feature complement(1459..1525)
/note="intron of SV40 small t-antigen"
CDS complement(1526..>1600)
/codon_start=1
/transl_table=11
/note="part of SV40 small t-antigen"
/protein_id="SJL88606.1"
/translation="DLCEGTLLLWCDIIGQTTYRDLKL"
misc_feature complement(1608..1857)
/note="3' UTR of mTNF"
CDS complement(1858..>2328)
/codon_start=1
/transl_table=11
/note="mTNF mature sequence"
/protein_id="SJL88607.1"
/translation="LRSSSQNSSDKPVAHVVANHQVEEQLEWLSQRANALLANGMDLK
DNQLVVPADGLYLVYSQVLFKGQGCPDYVLLTHTVSRFAISYQEKVNLLSAVKSPCPK
DTPEGAELKPWYEPIYLGGVFQLEKGDQLSAEVNLPKYLDFAESGQVYFGVIAL"
CDS complement(<2329..2400)
/transl_table=11
/note="mutated mIL6 signal sequence"
/protein_id="SJL88608.1"
/translation="MKFLSARDFHPVAFLGLMLVTTTA"
rep_origin complement(2449..2524)
/note="SV40 ori"
regulatory complement(2481..2762)
/note="SV40 early promoter"
/regulatory_class="promoter"
rep_origin complement(2984..3386)
/note="pMB1 ori"
CDS complement(3927..4787)
/transl_table=11
/note="ampicillin"
/protein_id="SJL88609.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
BASE COUNT 1318 a 1125 c 1228 g 1319 t
ORIGIN
1 ttcctttgcc taatttaaat gaggacttaa cctgtggaaa tattttgatg tgggaagctg
61 ttactgttaa aactgaggtt attggggtaa ctgctatgtt aaacttgcat tcagggacac
121 aaaaaactca tgaaaatggt gctggaaaac ccattcaagg gtcaaatttt catttttttg
181 ctgttggtgg ggaacctttg gagctgcagg gtgtgttagc aaactacagg accaaatatc
241 ctgctcaaac tgtaacccca aaaaatgcta cagttgacag tcagcagatg aacactgacc
301 acaaggctgt tttggataag gataatgctt atccagtgga gtgctgggtt cctgatccaa
361 gtaaaaatga aaacactaga tattttggaa cctacacagg tggggaaaat gtgcctcctg
421 ttttgcacat tactaacaca gcaaccacag tgcttcttga tgagcagggt gttgggccct
481 tgtgcaaagc tgacagcttg tatgtttctg ctgttgacat ttgtgggctg tttaccaaca
541 cttctggaac acagcagtgg aagggacttc ccagatattt taaaattacc cttagaaagc
601 ggtctgtgaa aaacccctac ccaatttcct ttttgttaag tgacctaatt aacaggagga
661 cacagagggt ggatgggcag cctatgattg gaatgtcctc tcaagtagag gaggttaggg
721 tttatgagga cacagaggag cttcctgggg atccagacat gataagatac attgatgagt
781 ttggacaaac cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg
841 ctattgcttt atttgtaacc attataagct gcaataaaca agttaacaac aacaattgca
901 ttcattttat gtttcaggtt cagggggagg tgtgggaggt tttttaaagc aagtaaaacc
961 tctacaaatg tggtatggct gattatgatc tctagtcaag gcactataca tcaaatattc
1021 cttattaacc cctttacaaa ttaaaaagct aaaggtacac aatttttgag catagttatt
1081 aatagcagac actctatgcc tgtgtggagt aagaaaaaac agtatgttat gattataact
1141 gttatgccta cttataaagg ttacagaata tttttccata attttcttgt atagcagtgc
1201 agctttttcc tttgtggtgt aaatagcaaa gcaagcaaga gttctattac taaacacagc
1261 atgactcaaa aaacttagca attctgaagg aaagtccttg gggtcttcta cctttctctt
1321 cttttttgga ggagtagaat gttgagagtc agcagtagcc tcatcatcac tagatggcat
1381 ttcttctgag caaaacaggt tttcctcatt aaaggcattc caccactgct cccattcatc
1441 agttccatag gttggaatct aaaatacaca aacaattaga atcagtagtt taacacatta
1501 tacacttaaa aattttatat ttaccttaga gctttaaatc tctgtaggta gtttgtccaa
1561 ttatgtcaca ccacagaagt aaggttcctt cacaaagatc cgtcgacnnn nnggaaagcc
1621 catttgagtc cttgatggtg gtgcatgaga ggcccacagt ccaggtcact gtcccagcat
1681 cttgtgtttc tgagtagttg ttgaaagctc tgagcacaga gttggactct gagccataat
1741 cccctttcta agttagaagg acacagactg ggggctctga ggagtagaca ataaaggggt
1801 cagagtaaag gggtcagagt gggggctggg tagagaatgg atgaacaccc attcccttca
1861 cagagcaatg actccaaagt agacctgccc ggactccgca aagtctaagt acttgggcag
1921 attgacctca gcgctgagtt ggtccccctt ctccagctgg aagactcctc ccaggtatat
1981 gggctcatac cagggtttga gctcagcccc ctcaggggtg tccttggggc aggggctctt
2041 gacggcagag aggaggttga ctttctcctg gtatgagata gcaaatcggc tgacggtgtg
2101 ggtgaggagc acgtagtcgg ggcagccttg tcccttgaag agaacctggg agtagacaag
2161 gtacaaccca tcggctggca ccactagttg gttgtctttg agatccatgc cgttggccag
2221 gagggcgttg gcgcgctggc tcagccactc cagctgctcc tccacttggt ggtttgctac
2281 gacgtgggct acaggcttgt cactcgaatt ttgagaagaa gatctgaggg ccgtggttgt
2341 caccagcatc agtcctaaga aggcaactgg atggaagtct cttgcagaga ggaacttcat
2401 agcggtttct ggaattgact atcgttcttg gtgggaattc ctccaaaaaa gcctcctcac
2461 tacttctgga atagctcaga ggccgaggcg gcctcggcct ctgcataaat aaaaaaaatt
2521 agtcagccat ggggcggaga atgggcggaa ctgggcggag ttaggggcgg gatgggcgga
2581 gttaggggcg ggactatggt tgctgactaa ttgagatgca tgctttgcat acttctgcct
2641 gctggggagc ctggggactt tccacacctg gttgctgact aattgagatg catgctttgc
2701 atacttctgc ctgctgggga gcctggggac tttccacacc ctaactgaca cacattccac
2761 agtcgggcag cgttgggtcc tggccacggg tgcgcatgat cgtgctcctg tcgttgagga
2821 cccggctagg ctggcggggt tgccttactg gttagcagaa tgaatcaccg atacgcgagc
2881 gaacgtgaag cgactgctgc tgcaaaacgt ctgcgacctg agcaacaaca tgaatggtct
2941 tcggtttccg tgtttcgtaa agtctggaaa cgcggaagtc agcgctcttc cgcttcctcg
3001 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
3061 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
3121 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
3181 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
3241 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
3301 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
3361 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
3421 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
3481 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
3541 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
3601 actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
3661 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
3721 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
3781 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
3841 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
3901 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
3961 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
4021 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
4081 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
4141 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
4201 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctgcaggcat cgtggtgtca
4261 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
4321 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
4381 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
4441 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
4501 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caacacggga taataccgcg
4561 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
4621 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
4681 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
4741 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
4801 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
4861 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
4921 gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc
4981 tttcgtcttc aagaa
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