LOCUS LT727478 3735 bp DNA circular SYN 06-FEB-2017
DEFINITION Vector pLF10T4, complete sequence.
ACCESSION LT727478
VERSION LT727478.1
KEYWORDS .
SOURCE Vector pLF10T4
ORGANISM Vector pLF10T4
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 3735)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..3735
/organism="Vector pLF10T4"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 3386."
/db_xref="taxon:1945323"
regulatory 24..40
/note="T7"
/regulatory_class="promoter"
misc_feature 41..103
/note="5UTR"
CDS 104..>146
/transl_table=11
/note="g10f"
/protein_id="SJL88590.1"
/translation="MASMTGGQQMGTNQ"
misc_feature 155..187
/note="H6EK"
regulatory complement(330..347)
/note="T3"
/regulatory_class="promoter"
regulatory 364..411
/note="T7T"
/regulatory_class="terminator"
regulatory 485..532
/note="T7T"
/regulatory_class="terminator"
regulatory 601..682
/note="fdT"
/regulatory_class="terminator"
regulatory 711..792
/note="fdT"
/regulatory_class="terminator"
rep_origin 890..1345
/note="f1 ori"
rep_origin complement(1349..1722)
/note="pMB1 ori"
CDS complement(2263..3123)
/transl_table=11
/note="ampicillin resistance"
/protein_id="SJL88591.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
regulatory 3489..3619
/note="PL"
/regulatory_class="promoter"
BASE COUNT 970 a 922 c 881 g 962 t
ORIGIN
1 ttccggatct cgatcccgga aattaatacg actcactata gggagaccac aacggtttcc
61 ctctagaaat aattttgttt aactttaaga aggagatata catatggcta gcatgactgg
121 tggacagcaa atgggtacta accaagcgga tccacatcac catcaccatc acgacgatga
181 cgataaggcg ccgacgtcgc atgctcctct agactcgagg aattcggtac cccgggttcg
241 aaatcgataa gcttatgcat gcggccgcat ctagagggcc cggatccctc gaggtcgacg
301 aattcgagct cggccgactt ggccttccct ttagtgaggg ttaataaact tggtgagcaa
361 taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga
421 ggaactatat gcgctcatac gatatgaacg ttgagactgc cgctgagtta tcagtgagca
481 ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg
541 aggaactata tccggatagc ttatcgctag aggtcgaaat tcacctcgaa agcaagctga
601 taaaccgata caattaaagg ctccttttgg agcctttttt tttggagatt ttcaacgtga
661 aaaaattatt attcgcaatt ccaagctaat tcacctcgaa agcaagctga taaaccgata
721 caattaaagg ctccttttgg agcctttttt tttggagatt ttcaacgtga aaaaattatt
781 attcgcaatt ccaagctctg cctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac
841 atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgcagatca cgcgccctgt
901 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
961 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
1021 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg
1081 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga
1141 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc
1201 caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg
1261 ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt
1321 aacaaaatat taacgtttac aatttgatct gcgctcggtc gttcggctgc ggcgagcggt
1381 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
1441 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
1501 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
1561 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
1621 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
1681 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
1741 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
1801 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
1861 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
1921 gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt
1981 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
2041 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
2101 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
2161 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
2221 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
2281 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
2341 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
2401 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
2461 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
2521 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctgc
2581 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
2641 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
2701 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
2761 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
2821 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaac
2881 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
2941 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
3001 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
3061 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
3121 catactcttc ctttttcaat attatgtaag cagacagttt tattgttcat gatgatatat
3181 ttttatcttg tgcaatgtaa catcagagat tttgagacac aacgtggctt tgttgaataa
3241 atcgaacttt tgctgagttg aaggatcaga tcacgcatct tcccgacaac gcagaccgtt
3301 ccgtggcaaa gcaaaagttc aaaatcacca actggtccac ctacaacaaa gctctcatca
3361 accgtggctc cctcactttc tggctggatg atggggcgat tcaggcctgg tatgagtcag
3421 caacaccttc ttcacgaggc agacctcagc gccggtgatg ccggccacga tgcgtccggc
3481 gtagaggatc tctcacctac caaacaatgc ccccctgcaa aaaataaatt catataaaaa
3541 acatacagat aaccatctgc ggtgataaat tatctctggc ggtgttgaca taaataccac
3601 tggcggtgat actgagcaca tcagcaggac gcactgacca ccatgaaggt gacgctctta
3661 aaattaagcc ctgaagaagg gcagcattca aagcagaagg ctttggggtg tgtgatacga
3721 aacgaagcat tggaa
//