LOCUS LT727477 3653 bp DNA circular SYN 06-FEB-2017
DEFINITION Vector pLT10TH, complete sequence.
ACCESSION LT727477
VERSION LT727477.1
KEYWORDS .
SOURCE Vector pLT10TH
ORGANISM Vector pLT10TH
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 3653)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..3653
/organism="Vector pLT10TH"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 3385."
/db_xref="taxon:1945331"
regulatory 24..40
/note="T7 promoter"
/regulatory_class="promoter"
misc_feature 41..103
/note="5' UTR of T7 gene 10, incl. RBS"
misc_feature 113..130
/note="His-tag"
misc_feature 131..145
/note="EK recognition site"
regulatory complement(248..264)
/note="T3 promoter"
/regulatory_class="promoter"
regulatory 282..329
/note="T7 terminator"
/regulatory_class="terminator"
regulatory 403..450
/note="T7 terminator"
/regulatory_class="terminator"
regulatory 519..600
/note="fd terminator"
/regulatory_class="terminator"
regulatory 629..710
/note="fd terminator"
/regulatory_class="terminator"
rep_origin 808..1263
/note="f1 ori"
rep_origin complement(1267..1640)
/note="pMB1 ori"
CDS complement(2181..3041)
/transl_table=11
/note="ampicillin resistance"
/protein_id="SJL88589.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
regulatory 3407..3537
/note="PL promoter"
/regulatory_class="promoter"
BASE COUNT 949 a 900 c 858 g 946 t
ORIGIN
1 ttccggatct cgatcccgga aattaatacg actcactata gggagaccac aacggtttcc
61 ctctagaaat aattttgttt aactttaaga aggagatata catatggatc cacatcacca
121 tcaccatcac gacgatgacg ataaggcgcc cgggttcgaa atcgataagc ttatgcatgc
181 ggccgcatct agagggcccg gatccctcga ggtcgacgaa ttcgagctcg gccgacttgg
241 ccttcccttt agtgagggtt aataaacttg gtgagcaata actagcataa ccccttgggg
301 cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatgc gctcatacga
361 tatgaacgtt gagactgccg ctgagttatc agtgagcaat aactagcata accccttggg
421 gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatagctt
481 atcgctagag gtcgaaattc acctcgaaag caagctgata aaccgataca attaaaggct
541 ccttttggag cctttttttt tggagatttt caacgtgaaa aaattattat tcgcaattcc
601 aagctaattc acctcgaaag caagctgata aaccgataca attaaaggct ccttttggag
661 cctttttttt tggagatttt caacgtgaaa aaattattat tcgcaattcc aagctctgcc
721 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
781 cagcttgtct gtaagcggat gcagatcacg cgccctgtag cggcgcatta agcgcggcgg
841 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt
901 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
961 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
1021 attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga
1081 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc
1141 ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa
1201 aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta acgtttacaa
1261 tttgatctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
1321 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
1381 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
1441 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
1501 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
1561 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
1621 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
1681 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
1741 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
1801 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
1861 aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
1921 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
1981 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
2041 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
2101 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
2161 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
2221 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
2281 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
2341 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
2401 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
2461 ccagttaata gtttgcgcaa cgttgttgcc attgctgcag gcatcgtggt gtcacgctcg
2521 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
2581 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
2641 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
2701 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
2761 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaacac gggataatac cgcgccacat
2821 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
2881 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
2941 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
3001 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
3061 tatgtaagca gacagtttta ttgttcatga tgatatattt ttatcttgtg caatgtaaca
3121 tcagagattt tgagacacaa cgtggctttg ttgaataaat cgaacttttg ctgagttgaa
3181 ggatcagatc acgcatcttc ccgacaacgc agaccgttcc gtggcaaagc aaaagttcaa
3241 aatcaccaac tggtccacct acaacaaagc tctcatcaac cgtggctccc tcactttctg
3301 gctggatgat ggggcgattc aggcctggta tgagtcagca acaccttctt cacgaggcag
3361 acctcagcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatctc tcacctacca
3421 aacaatgccc ccctgcaaaa aataaattca tataaaaaac atacagataa ccatctgcgg
3481 tgataaatta tctctggcgg tgttgacata aataccactg gcggtgatac tgagcacatc
3541 agcaggacgc actgaccacc atgaaggtga cgctcttaaa attaagccct gaagaagggc
3601 agcattcaaa gcagaaggct ttggggtgtg tgatacgaaa cgaagcattg gaa
//