LOCUS LT727476 3654 bp DNA circular SYN 06-FEB-2017
DEFINITION Vector pLT10T3, complete sequence.
ACCESSION LT727476
VERSION LT727476.1
KEYWORDS .
SOURCE Vector pLT10T3
ORGANISM Vector pLT10T3
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 3654)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..3654
/organism="Vector pLT10T3"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 3384."
/db_xref="taxon:1945329"
regulatory 24..40
/note="T7 promoter"
/regulatory_class="promoter"
misc_feature 41..103
/note="5' UTR of T7 gene 10, incl. RBS"
regulatory complement(249..265)
/note="T3 promoter"
/regulatory_class="promoter"
regulatory 283..330
/note="T7 terminator"
/regulatory_class="terminator"
regulatory 404..451
/note="T7 terminator"
/regulatory_class="terminator"
regulatory 520..601
/note="fd terminator"
/regulatory_class="terminator"
regulatory 630..711
/note="fd terminator"
/regulatory_class="terminator"
rep_origin 809..1264
/note="f1 ori"
rep_origin complement(1268..1641)
/note="pMB1 ori"
CDS complement(2182..3042)
/transl_table=11
/note="ampicillin resistance"
/protein_id="SJL88588.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
regulatory 3408..3538
/note="PL promoter"
/regulatory_class="promoter"
BASE COUNT 943 a 900 c 862 g 949 t
ORIGIN
1 ttccggatct cgatcccgga aattaatacg actcactata gggagaccac aacggtttcc
61 ctctagaaat aattttgttt aactttaaga aggagatata catatgggcc cgacgtcgca
121 tgctcctcta gactcgagga attcggtacc ccgggttcga aatcgataag cttatgcatg
181 cggccgcatc tagagggccc ggatccctcg aggtcgacga attcgagctc ggccgacttg
241 gccttccctt tagtgagggt taataaactt ggtgagcaat aactagcata accccttggg
301 gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatg cgctcatacg
361 atatgaacgt tgagactgcc gctgagttat cagtgagcaa taactagcat aaccccttgg
421 ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatagct
481 tatcgctaga ggtcgaaatt cacctcgaaa gcaagctgat aaaccgatac aattaaaggc
541 tccttttgga gccttttttt ttggagattt tcaacgtgaa aaaattatta ttcgcaattc
601 caagctaatt cacctcgaaa gcaagctgat aaaccgatac aattaaaggc tccttttgga
661 gccttttttt ttggagattt tcaacgtgaa aaaattatta ttcgcaattc caagctctgc
721 ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc
781 acagcttgtc tgtaagcgga tgcagatcac gcgccctgta gcggcgcatt aagcgcggcg
841 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct
901 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
961 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
1021 gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
1081 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
1141 cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta
1201 aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgtttaca
1261 atttgatctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
1321 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
1381 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
1441 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
1501 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
1561 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
1621 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
1681 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
1741 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
1801 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
1861 aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
1921 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
1981 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
2041 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
2101 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
2161 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
2221 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
2281 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
2341 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
2401 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
2461 gccagttaat agtttgcgca acgttgttgc cattgctgca ggcatcgtgg tgtcacgctc
2521 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
2581 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
2641 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
2701 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
2761 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaaca cgggataata ccgcgccaca
2821 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
2881 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
2941 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
3001 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
3061 ttatgtaagc agacagtttt attgttcatg atgatatatt tttatcttgt gcaatgtaac
3121 atcagagatt ttgagacaca acgtggcttt gttgaataaa tcgaactttt gctgagttga
3181 aggatcagat cacgcatctt cccgacaacg cagaccgttc cgtggcaaag caaaagttca
3241 aaatcaccaa ctggtccacc tacaacaaag ctctcatcaa ccgtggctcc ctcactttct
3301 ggctggatga tggggcgatt caggcctggt atgagtcagc aacaccttct tcacgaggca
3361 gacctcagcg ccggtgatgc cggccacgat gcgtccggcg tagaggatct ctcacctacc
3421 aaacaatgcc cccctgcaaa aaataaattc atataaaaaa catacagata accatctgcg
3481 gtgataaatt atctctggcg gtgttgacat aaataccact ggcggtgata ctgagcacat
3541 cagcaggacg cactgaccac catgaaggtg acgctcttaa aattaagccc tgaagaaggg
3601 cagcattcaa agcagaaggc tttggggtgt gtgatacgaa acgaagcatt ggaa
//