LOCUS LT727471 4316 bp DNA circular SYN 06-FEB-2017
DEFINITION Vector YDp-L, complete sequence.
ACCESSION LT727471
VERSION LT727471.1
KEYWORDS .
SOURCE Vector YDp-L
ORGANISM Vector YDp-L
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 4316)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..4316
/organism="Vector YDp-L"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 3355."
/db_xref="taxon:1945441"
CDS 837..1931
/transl_table=11
/note="LEU2"
/protein_id="SJL88579.1"
/translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENH
LIGGAAIDATGVPLPDEALEASKKVDAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQL
YANLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSE
QYTVPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLK
VQHQLIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPD
KNTAFGLYEPCHGSAPDLPKNKVDPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLD
AGIRTGDLGGSNSTTEVGDAVAEEVKKILA"
regulatory complement(2138..2221)
/note="lac promoter"
/regulatory_class="promoter"
rep_origin complement(2313..2715)
/note="pMB1 ori"
CDS complement(3256..4116)
/transl_table=11
/note="ampicillin resistance"
/protein_id="SJL88580.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
BASE COUNT 1147 a 996 c 1029 g 1144 t
ORIGIN
1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cccggggatc cggtgattga
421 ttgagcaagc taactgtggg aatactcagg tatcgtaaga tgcaagagtt cgaatctctt
481 agcaaccatt atttttttcc tcaacataac gagaacacac aggggcgcta tcgcacagaa
541 tcaaattcga tgactggaaa ttttttgtta atttcagagg tcgcctgacg catatacctt
601 tttcaactga aaaattggga gaaaaaggaa aggtgagagg ccggaaccgg cttttcatat
661 agaatagaga agcgttcatg actaaatgct tgcatcacaa tacttgaagt tgacaatatt
721 atttaaggac ctattgtttt ttccaatagg tggttagcaa tcgtcttact ttctaacttt
781 tcttaccttt tacatttcag caatatatat atatatttca aggatatacc attctaatgt
841 ctgcccctaa gaagatcgtc gttttgccag gtgaccacgt tggtcaagaa atcacagccg
901 aagccattaa ggttcttaaa gctatttctg atgttcgttc caatgtcaag ttcgatttcg
961 aaaatcattt aattggtggt gctgctatcg atgctacagg tgtcccactt ccagatgagg
1021 cgctggaagc ctccaagaag gttgatgccg ttttgttagg tgctgtgggt ggtcctaaat
1081 ggggtaccgg tagtgttaga cctgaacaag gtttactaaa aatccgtaaa gaacttcaat
1141 tgtacgccaa cttaagacca tgtaactttg catccgactc tcttttagac ttatctccaa
1201 tcaagccaca atttgctaaa ggtactgact tcgttgttgt cagagaatta gtgggaggta
1261 tttactttgg taagagaaag gaagacgatg gtgatggtgt cgcttgggat agtgaacaat
1321 acaccgttcc agaagtgcaa agaatcacaa gaatggccgc tttcatggcc ctacaacatg
1381 agccaccatt gcctatttgg tccttggata aagctaatgt tttggcctct tcaagattat
1441 ggagaaaaac tgtggaggaa accatcaaga acgaattccc tacattgaag gttcaacatc
1501 aattgattga ttctgccgcc atgatcctag ttaagaaccc aacccaccta aatggtatta
1561 taatcaccag caacatgttt ggtgatatca tctccgatga agcctccgtt atcccaggtt
1621 ccttgggttt gttgccatct gcgtccttgg cctctttgcc agacaagaac accgcatttg
1681 gtttgtacga accatgccac ggttctgctc cagatttgcc aaagaataag gttgacccta
1741 tcgccactat cttgtctgct gcaatgatgt tgaaattgtc attgaacttg cctgaagaag
1801 gtaaggccat tgaagatgca gttaaaaagg ttttggatgc aggtatcaga actggtgatt
1861 taggtggttc caacagtacc accgaagtcg gtgatgctgt cgccgaagaa gttaagaaaa
1921 tccttgctta aaaagattct ctttttttat gatatttgta cataaacttt ataaatgaaa
1981 ttcataatag aaacgacacg aaattacaaa atggaatatg ttcatagggt agagcttgct
2041 caatcaatca ccggatccgt cgacctgcag ccaagctagc ttggcgtaat catggtcata
2101 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
2161 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
2221 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
2281 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
2341 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
2401 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
2461 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
2521 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
2581 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
2641 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
2701 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
2761 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
2821 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
2881 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac
2941 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
3001 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
3061 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
3121 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
3181 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
3241 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
3301 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
3361 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
3421 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
3481 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
3541 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
3601 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
3661 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
3721 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
3781 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
3841 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
3901 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
3961 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
4021 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
4081 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
4141 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
4201 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac
4261 cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtc
//