LOCUS LT727469 6252 bp DNA circular SYN 06-FEB-2017
DEFINITION Vector pMxhFasR, complete sequence.
ACCESSION LT727469
VERSION LT727469.1
KEYWORDS .
SOURCE Vector pMxhFasR
ORGANISM Vector pMxhFasR
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 6252)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..6252
/organism="Vector pMxhFasR"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 3345."
/db_xref="taxon:1945359"
regulatory 13..458
/note="Mx"
/regulatory_class="promoter"
misc_feature 484..681
/note="5UTR"
CDS 682..1689
/transl_table=11
/note="hFasR"
/protein_id="SJL88572.1"
/translation="MLGIWTLLPLVLTSVARLSSKSVNAQVTDINSKGLELRKTVTTV
ETQNLEGLHHDGQFCHKPCPPGERKARDCTVNGDEPDCVPCQEGKEYTDKAHFSSKCR
RCRLCDEGHGLEVEINCTRTQNTKCRCKPNFFCNSTVCEHCDPCTKCEHGIIKECTLT
SNTKCKEEGSRSNLGWLCLLLLPIPLIVWVKRKEVQKTCRKHRKENQGSHESPTLNPE
TVAINLSDVDLSKYITTIAGVMTLSQVKGFVRKNGVNEAKIDEIKNDNVQDTAEQKVQ
LLRNWHQLHGKKEAYDTLIKDLKKANLCTLAEKIQTIILKDITSDSENSNFRNEIQSL
V"
CDS <1698..1772
/codon_start=1
/transl_table=11
/note="part of SV40 small t-antigen"
/protein_id="SJL88573.1"
/translation="DLCEGTLLLWCDIIGQTTYRDLKL"
misc_feature 1773..1839
/note="intron of SV40 small t-antigen"
misc_feature 1840..2391
/note="3' UTR of SV40 small t-antigen"
regulatory 2392..2488
/note="polyA"
/regulatory_class="terminator"
regulatory complement(2392..2488)
/note="polyA"
/regulatory_class="terminator"
misc_feature complement(2489..3301)
/note="part of SV40 VP1"
regulatory complement(3357..3440)
/note="lac"
/regulatory_class="promoter"
rep_origin complement(3535..3935)
/note="pMB1 ori"
CDS complement(4475..5335)
/transl_table=11
/note="ampicillin resistance"
/protein_id="SJL88574.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
regulatory 6230..6246
/note="SP6"
/regulatory_class="promoter"
BASE COUNT 1710 a 1465 c 1464 g 1610 t
ORIGIN
1 aagcttgggc tgcagtagca gtgctcagac ctctaaacct gtagtcacaa gggacccaga
61 agggctggca gggctcctca gtgattctgg tatggtttgt ctctgaccct tcctagtgca
121 gctggaagaa tccagtgaaa agagaaagtt agtctaggtt gtgttcaact gagaggaggt
181 caggctgaag gctcagcaca gagctggaag ggtcctggga aagcaggcag ccatctgaac
241 tggtgtccat actgcatgtg tcttggtggg ctgagcctac aatatcgatt tcccaacctc
301 agtaccaagc caaggcccct cagggtgagt ttcgtttctg agctcctttc atatgtggtg
361 ggggtgtggg cacagcaagc tcaggctttt tcacggtttc aattctcctc tggaaggggc
421 ggagccagca ctatttaaag aggtctgagc cctggcagga ctctagagat ccctcgacct
481 cgagnnngac gcttctgggg agtgagggaa gcggtttacg agtgacttgg ctggagcctc
541 aggggcgggc actggcacgg aacacaccct gaggccagcc ctggctgccc aggcggagct
601 gcctcttctc ccgcgggttg gtggacccgc tcagtacgga gttggggaag ctctttcact
661 tcggaggatt gctcaacaac catgctgggc atctggaccc tcctacctct ggttcttacg
721 tctgttgcta gattatcgtc caaaagtgtt aatgcccaag tgactgacat caactccaag
781 ggattggaat tgaggaagac tgttactaca gttgagactc agaacttgga aggcctgcat
841 catgatggcc aattctgcca taagccctgt cctccaggtg aaaggaaagc tagggactgc
901 acagtcaatg gggatgaacc agactgcgtg ccctgccaag aagggaagga gtacacagac
961 aaagcccatt tttcttccaa atgcagaaga tgtagattgt gtgatgaagg acatggctta
1021 gaagtggaaa taaactgcac ccggacccag aataccaagt gcagatgtaa accaaacttt
1081 ttttgtaact ctactgtatg tgaacactgt gacccttgca ccaaatgtga acatggaatc
1141 atcaaggaat gcacactcac cagcaacacc aagtgcaaag aggaaggatc cagatctaac
1201 ttggggtggc tttgtcttct tcttttgcca attccactaa ttgtttgggt gaagagaaag
1261 gaagtacaga aaacatgcag aaagcacaga aaggaaaacc aaggttctca tgaatctcca
1321 accttaaatc ctgaaacagt ggcaataaat ttatctgatg ttgacttgag taaatatatc
1381 accactattg ctggagtcat gacactaagt caagttaaag gctttgttcg aaagaatggt
1441 gtcaatgaag ccaaaataga tgagatcaag aatgacaatg tccaagacac agcagaacag
1501 aaagttcaac tgcttcgtaa ttggcatcaa cttcatggaa agaaagaagc gtatgacaca
1561 ttgattaaag atctcaaaaa agccaatctt tgtactcttg cagagaaaat tcagactatc
1621 atcctcaagg acattactag tgactcagaa aattcaaact tcagaaatga aatccaaagc
1681 ttggtctaga gtcgacggat ctttgtgaag gaaccttact tctgtggtgt gacataattg
1741 gacaaactac ctacagagat ttaaagctct aaggtaaata taaaattttt aagtgtataa
1801 tgtgttaaac tactgattct aattgtttgt gtattttaga ttccaaccta tggaactgat
1861 gaatgggagc agtggtggaa tgcctttaat gaggaaaacc tgttttgctc agaagaaatg
1921 ccatctagtg atgatgaggc tactgctgac tctcaacatt ctactcctcc aaaaaagaag
1981 agaaaggtag aagaccccaa ggactttcct tcagaattgc taagtttttt gagtcatgct
2041 gtgtttagta atagaactct tgcttgcttt gctatttaca ccacaaagga aaaagctgca
2101 ctgctataca agaaaattat ggaaaaatat tctgtaacct ttataagtag gcataacagt
2161 tataatcata acatactgtt ttttcttact ccacacaggc atagagtgtc tgctattaat
2221 aactatgctc aaaaattgtg tacctttagc tttttaattt gtaaaggggt taataaggaa
2281 tatttgatgt atagtgcctt gactagagat cataatcagc cataccacat ttgtagaggt
2341 tttacttgct ttaaaaaacc tcccacacct ccccctgaac ctgaaacata aaatgaatgc
2401 aattgttgtt gttaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
2461 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
2521 catcaatgta tcttatcatg tctggatcga tccccaggaa gctcctctgt gtcctcataa
2581 accctaacct cctctacttg agaggacatt ccaatcatag gctgcccatc caccctctgt
2641 gtcctcctgt taattaggtc acttaacaaa aaggaaattg ggtaggggtt tttcacagac
2701 cgctttctaa gggtaatttt aaaatatctg ggaagtccct tccactgctg tgttccagaa
2761 gtgttggtaa acagcccaca aatgtcaaca gcagaaacat acaagctgtc agctttgcac
2821 aagggcccaa caccctgctc atcaagaagc actgtggttg ctgtgttagt aatgtgcaaa
2881 acaggaggca cattttcccc acctgtgtag gttccaaaat atctagtgtt ttcattttta
2941 cttggatcag gaacccagca ctccactgga taagcattat ccttatccaa aacagccttg
3001 tggtcagtgt tcatctgctg actgtcaact gtagcatttt ttggggttac agtttgagca
3061 ggatatttgg tcctgtagtt tgctaacaca ccctgcagct ccaaaggttc cccaccaaca
3121 gcaaaaaaat gaaaatttga cccttgaatg ggttttccag caccattttc atgagttttt
3181 tgtgtccctg aatgcaagtt taacatagca gttaccccaa taacctcagt tttaacagta
3241 acagcttccc acatcaaaat atttccacag gttaagtcct catttaaatt aggcaaagga
3301 attcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac
3361 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac
3421 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc
3481 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg
3541 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
3601 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
3661 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
3721 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
3781 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
3841 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
3901 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
3961 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
4021 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
4081 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
4141 acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
4201 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
4261 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
4321 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
4381 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
4441 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
4501 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
4561 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
4621 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
4681 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
4741 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
4801 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
4861 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
4921 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
4981 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
5041 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
5101 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
5161 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
5221 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
5281 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
5341 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
5401 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
5461 cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca
5521 cgaggccctt tcgtctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc
5581 tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg
5641 gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa ctatgcggca tcagagcaga
5701 ttgtactgag agtgcaccat atcgacgctc tcccttatgc gactcctgca ttaggaagca
5761 gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga
5821 gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc
5881 gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc
5941 gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat
6001 ctggctagcg atgaccctgc tgattggttc gctgaccatt tccggggtgc ggaacggcgt
6061 taccagaaac tcagaaggtt cgtccaacca aaccgactct gacggcagtt tacgagagag
6121 atgatagggt ctgcttcagt aagccagatg ctacacaatt aggcttgtac atattgtcgt
6181 tagaacgcgg ctacaattaa tacataacct tatgtatcat acacatacga tttaggtgac
6241 actatagaat ac
//