LOCUS LT727465 5329 bp DNA circular SYN 06-FEB-2017
DEFINITION Vector pLT10LUCT3, complete sequence.
ACCESSION LT727465
VERSION LT727465.1
KEYWORDS .
SOURCE Vector pLT10LUCT3
ORGANISM Vector pLT10LUCT3
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 5329)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..5329
/organism="Vector pLT10LUCT3"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 3326."
/db_xref="taxon:1945328"
regulatory 24..40
/note="T7"
/regulatory_class="promoter"
misc_feature 41..103
/note="5UTR"
CDS 104..1756
/transl_table=11
/note="LUC"
/protein_id="SJL88559.1"
/translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTD
AHIEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAV
APANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQG
FQSMYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVR
FSHARDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRS
LQDYKIQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLP
GIRQGYGLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCV
RGPMIMSGYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVA
PAELESILLQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTT
AKKLRGGVVFVDEVPKGLTGKLDARKIREILIKAKKGGKSKL"
misc_feature 1757..1892
/note="3UTR"
regulatory complement(1924..1941)
/note="T3"
/regulatory_class="promoter"
regulatory 1958..2005
/note="T7T"
/regulatory_class="terminator"
regulatory 2079..2126
/note="T7T"
/regulatory_class="terminator"
regulatory 2195..2276
/note="fdT"
/regulatory_class="terminator"
regulatory 2305..2386
/note="fdT"
/regulatory_class="terminator"
rep_origin 2484..2939
/note="f1 ori"
rep_origin complement(2943..3316)
/note="pMB1 ori"
CDS complement(3857..4717)
/transl_table=11
/note="ampicillin resistance"
/protein_id="SJL88560.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
regulatory 5083..5213
/note="PL"
/regulatory_class="promoter"
BASE COUNT 1450 a 1224 c 1247 g 1408 t
ORIGIN
1 ttccggatct cgatcccgga aattaatacg actcactata gggagaccac aacggtttcc
61 ctctagaaat aattttgttt aactttaaga aggagatata catatggaag acgccaaaaa
121 cataaagaaa ggcccggcgc cattctatcc tctagaggat ggaaccgctg gagagcaact
181 gcataaggct atgaagagat acgccctggt tcctggaaca attgctttta cagatgcaca
241 tatcgaggtg aacatcacgt acgcggaata cttcgaaatg tccgttcggt tggcagaagc
301 tatgaaacga tatgggctga atacaaatca cagaatcgtc gtatgcagtg aaaactctct
361 tcaattcttt atgccggtgt tgggcgcgtt atttatcgga gttgcagttg cgcccgcgaa
421 cgacatttat aatgaacgtg aattgctcaa cagtatgaac atttcgcagc ctaccgtagt
481 gtttgtttcc aaaaaggggt tgcaaaaaat tttgaacgtg caaaaaaaat taccaataat
541 ccagaaaatt attatcatgg attctaaaac ggattaccag ggatttcagt cgatgtacac
601 gttcgtcaca tctcatctac ctcccggttt taatgaatac gattttgtac cagagtcctt
661 tgatcgtgac aaaacaattg cactgataat gaattcctct ggatctactg ggttacctaa
721 gggtgtggcc cttccgcata gaactgcctg cgtcagattc tcgcatgcca gagatcctat
781 ttttggcaat caaatcattc cggatactgc gattttaagt gttgttccat tccatcacgg
841 ttttggaatg tttactacac tcggatattt gatatgtgga tttcgagtcg tcttaatgta
901 tagatttgaa gaagagctgt ttttacgatc ccttcaggat tacaaaattc aaagtgcgtt
961 gctagtacca accctatttt cattcttcgc caaaagcact ctgattgaca aatacgattt
1021 atctaattta cacgaaattg cttctggggg cgcacctctt tcgaaagaag tcggggaagc
1081 ggttgcaaaa cgcttccatc ttccagggat acgacaagga tatgggctca ctgagactac
1141 atcagctatt ctgattacac ccgaggggga tgataaaccg ggcgcggtcg gtaaagttgt
1201 tccatttttt gaagcgaagg ttgtggatct ggataccggg aaaacgctgg gcgttaatca
1261 gagaggcgaa ttatgtgtca gaggacctat gattatgtcc ggttatgtaa acaatccgga
1321 agcgaccaac gccttgattg acaaggatgg atggctacat tctggagaca tagcttactg
1381 ggacgaagac gaacacttct tcatagttga ccgcttgaag tctttaatta aatacaaagg
1441 atatcaggtg gcccccgctg aattggaatc gatattgtta caacacccca acatcttcga
1501 cgcgggcgtg gcaggtcttc ccgacgatga cgccggtgaa cttcccgccg ccgttgttgt
1561 tttggagcac ggaaagacga tgacggaaaa agagatcgtg gattacgtcg ccagtcaagt
1621 aacaaccgcg aaaaagttgc gcggaggagt tgtgtttgtg gacgaagtac cgaaaggtct
1681 taccggaaaa ctcgacgcaa gaaaaatcag agagatcctc ataaaggcca agaagggcgg
1741 aaagtccaaa ttgtaaaatg taactgtatt cagcgatgac gaaattctta gctattgtaa
1801 tattatatgc aaattgatga atggtaattt tgtaattgtg ggtcactgta ctattttaac
1861 gaataataaa atcaggtata ggtaactaaa aaggaattcg agctcggccg acttggcctt
1921 ccctttagtg agggttaata aacttggtga gcaataacta gcataacccc ttggggcctc
1981 taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatgcgctc atacgatatg
2041 aacgttgaga ctgccgctga gttatcagtg agcaataact agcataaccc cttggggcct
2101 ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tagcttatcg
2161 ctagaggtcg aaattcacct cgaaagcaag ctgataaacc gatacaatta aaggctcctt
2221 ttggagcctt tttttttgga gattttcaac gtgaaaaaat tattattcgc aattccaagc
2281 taattcacct cgaaagcaag ctgataaacc gatacaatta aaggctcctt ttggagcctt
2341 tttttttgga gattttcaac gtgaaaaaat tattattcgc aattccaagc tctgcctcgc
2401 gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc
2461 ttgtctgtaa gcggatgcag atcacgcgcc ctgtagcggc gcattaagcg cggcgggtgt
2521 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc
2581 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg
2641 gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta
2701 gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt
2761 ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat
2821 ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa
2881 tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgt ttacaatttg
2941 atctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
3001 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
3061 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
3121 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
3181 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
3241 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
3301 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
3361 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
3421 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
3481 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga
3541 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
3601 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
3661 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
3721 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
3781 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
3841 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
3901 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
3961 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
4021 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
4081 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
4141 ttaatagttt gcgcaacgtt gttgccattg ctgcaggcat cgtggtgtca cgctcgtcgt
4201 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
4261 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
4321 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
4381 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
4441 tgcggcgacc gagttgctct tgcccggcgt caacacggga taataccgcg ccacatagca
4501 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
4561 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
4621 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
4681 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatg
4741 taagcagaca gttttattgt tcatgatgat atatttttat cttgtgcaat gtaacatcag
4801 agattttgag acacaacgtg gctttgttga ataaatcgaa cttttgctga gttgaaggat
4861 cagatcacgc atcttcccga caacgcagac cgttccgtgg caaagcaaaa gttcaaaatc
4921 accaactggt ccacctacaa caaagctctc atcaaccgtg gctccctcac tttctggctg
4981 gatgatgggg cgattcaggc ctggtatgag tcagcaacac cttcttcacg aggcagacct
5041 cagcgccggt gatgccggcc acgatgcgtc cggcgtagag gatctctcac ctaccaaaca
5101 atgcccccct gcaaaaaata aattcatata aaaaacatac agataaccat ctgcggtgat
5161 aaattatctc tggcggtgtt gacataaata ccactggcgg tgatactgag cacatcagca
5221 ggacgcactg accaccatga aggtgacgct cttaaaatta agccctgaag aagggcagca
5281 ttcaaagcag aaggctttgg ggtgtgtgat acgaaacgaa gcattggaa
//