LOCUS LT727452 9277 bp DNA circular SYN 06-FEB-2017 DEFINITION Cloning vector pKT240tet2, complete sequence. ACCESSION LT727452 VERSION LT727452.1 KEYWORDS . SOURCE Cloning vector pKT240tet2 ORGANISM Cloning vector pKT240tet2 other sequences; artificial sequences; vectors. REFERENCE 1 (bases 1 to 9277) AUTHORS De Schamphelaire W. JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent, Technologiepark 927, 9052, BELGIUM. REFERENCE 2 AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K., Roggeman Fonseca M., Vanhoucke M., Beyaert R. TITLE BCCM/LMBP Plasmid collection JOURNAL Unpublished. FEATURES Location/Qualifiers source 1..9277 /organism="Cloning vector pKT240tet2" /mol_type="other DNA" /note="BCCM/LMBP Plasmid collection (Ghent University,Belgium) accession number LMBP 3226." /db_xref="taxon:1944718" CDS 86..1276 /transl_table=11 /note="tetracycline resistance" /protein_id="SJL88501.1" /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASH YGVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAG RIVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLH APFLAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTV FFIMQLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGE KQAIIAGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQG QLQGSLAALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSR ATST" rep_origin 2748..3143 /note="RSF1010 ori" CDS complement(3168..3452) /transl_table=11 /note="mobC" /protein_id="SJL88502.1" /translation="MVKGSNKAADRLAKLEEQRARINAEIQRVRAREQQQERKNETRR KVLVGAMILAKVNSSEWPEDRLMAAMDAYLERDHDRALFGLPPRQKDEPG" rep_origin 3483..3570 /note="RSF1010 oriT" CDS 3651..5780 /transl_table=11 /note="mobA" /protein_id="SJL88503.1" /translation="MAIYHLTAKTGSRSGGQSARAKADYIQREGKYARDMDEVLHAES GHMPEFVERPADYWDAADLYERANGRLFKEVEFALPVELTLDQQKALASEFAQHLTGA ERLPYTLAIHAGGGENPHCHLMISERINDGIERPAAQWFKRYNGKTPEKGGAQKTEAL KPKAWLEQTREAWADHANRALERAGHDARIDHRTLEAQGIERLPGVHLGPNVVEMEGR GIRTDRADVALNIDTANAQIIDLQEYREAIDHERNRQSEEIQRHQRVSGADRTAGPEH GDTGRRSPAGHEPDPAGQRGAGGGVAESPAPDRGGMGGAGQRVAGGSRRGEQRRAERP ERVAGVALEAMANRDAGFHDAYGGAADRIVALARPDATDNRGRLDLAALGGPMKNDRT LQAIGRQLKAMGCERFDIGVRDATTGQMMNREWSAAEVLQNTPWLKRMNAQGNDVYIR PAEQERHGLVLVDDLSEFDLDDMKAEGREPALVVETSPKNYQAWVKVADAAGGELRGQ IARTLASEYDADPASADSRHYGRLAGFTNRKDKHTTRAGYQPWVLLRESKGKTATAGP ALVQQAGQQIEQAQRQQEKARRLASLELPERQLSRHRRTALDEYRSEMAGLVKRFGDD LSKCDFIAAQKLASRGRSAEEIGKAMAEASPALAERKPGHEADYIERTVSKVMGLPSV QLARAELARAPAPRQRGMDRGGPDFSM" CDS 4399..4812 /transl_table=11 /note="mobB" /protein_id="SJL88504.1" /translation="MNAIDRVKKSRGINELAEQIEPLAQSMATLADEARQVMSQTQQA SEAQAAEWLKAQRQTGAAWVELAKELREVAAEVSSAAQSARSASRGWHWKLWLTVMLA SMMPTVVLLIASLLLLDLTPLTTEDGSIWLRLVAR" CDS 4809..5780 /transl_table=11 /note="repB" /protein_id="SJL88505.1" /translation="MKNDRTLQAIGRQLKAMGCERFDIGVRDATTGQMMNREWSAAEV LQNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDLSEFDLDDMKAEGREPALVVETSP KNYQAWVKVADAAGGELRGQIARTLASEYDADPASADSRHYGRLAGFTNRKDKHTTRA GYQPWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQEKARRLASLELPERQLSRHRR TALDEYRSEMAGLVKRFGDDLSKCDFIAAQKLASRGRSAEEIGKAMAEASPALAERKP GHEADYIERTVSKVMGLPSVQLARAELARAPAPRQRGMDRGGPDFSM" CDS 6055..6261 /transl_table=11 /note="rpsF" /protein_id="SJL88506.1" /translation="MKDQKDKQTGDLLASPDAVRQARYAERMKAKGMRQRKFWLTDDE YEALRECLEELRAAQGGGSDPASA" CDS 6291..7130 /transl_table=11 /note="repA" /protein_id="SJL88507.1" /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKS MLALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAV ADGLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVI GRMEAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSA EAEEWGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPR GEA" CDS 7117..7968 /transl_table=11 /note="repC" /protein_id="SJL88508.1" /translation="MVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYD YGDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWE AVTAECHVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRL LSEYASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWI DPGKTGKASIDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDI TRPKAAG" misc_feature 7914..8170 /note="RSF'" BASE COUNT 1970 a 2816 c 2717 g 1774 t ORIGIN 1 ttctcatgtt tgacagctta tcatcgataa gctttaatgc ggtagtttat cacagttaaa 61 ttgctaacgc agtcaggcac cgtgtatgaa atctaacaat gcgctcatcg tcatcctcgg 121 caccgtcacc ctggatgctg taggcatagg cttggttatg ccggtactgc cgggcctctt 181 gcgggatatc gtccattccg acagcatcgc cagtcactat ggcgtgctgc tagcgctata 241 tgcgttgatg caatttctat gcgcacccgt tctcggagca ctgtccgacc gctttggccg 301 ccgcccagtc ctgctcgctt cgctacttgg agccactatc gactacgcga tcatggcgac 361 cacacccgtc ctgtggatcc tctacgccgg acgcatcgtg gccggcatca ccggcgccac 421 aggtgcggtt gctggcgcct atatcgccga catcaccgat ggggaagatc gggctcgcca 481 cttcgggctc atgagcgctt gtttcggcgt gggtatggtg gcaggccccg tggccggggg 541 actgttgggc gccatctcct tgcatgcacc attccttgcg gcggcggtgc tcaacggcct 601 caacctacta ctgggctgct tcctaatgca ggagtcgcat aagggagagc gtcgaccgat 661 gcccttgaga gccttcaacc cagtcagctc cttccggtgg gcgcggggca tgactatcgt 721 cgccgcactt atgactgtct tctttatcat gcaactcgta ggacaggtgc cggcagcgct 781 ctgggtcatt ttcggcgagg accgctttcg ctggagcgcg acgatgatcg gcctgtcgct 841 tgcggtattc ggaatcttgc acgccctcgc tcaagccttc gtcactggtc ccgccaccaa 901 acgtttcggc gagaagcagg ccattatcgc cggcatggcg gccgacgcgc tgggctacgt 961 cttgctggcg ttcgcgacgc gaggctggat ggccttcccc attatgattc ttctcgcttc 1021 cggcggcatc gggatgcccg cgttgcaggc catgctgtcc aggcaggtag atgacgacca 1081 tcagggacag cttcaaggat cgctcgcggc tcttaccagc ctaacttcga tcactggacc 1141 gctgatcgtc acggcgattt atgccgcctc ggcgagcaca tggaacgggt tggcatggat 1201 tgtaggcgcc gccctatacc ttgtctgcct ccccgcgttg cgtcgcggtg catggagccg 1261 ggccacctcg acctgaatgg aagccggcgg cacctcgcta acggattcac cactccaaga 1321 attggagcca atcaattctt gcggagaact gtgaatgcgc aaaccaaccc ttggcagaac 1381 atatccatcg cgtccgccat ctccagcagc cgcacgcggc gcatctcggg cagcgttggg 1441 tcctggccac gggtgcgcat gatcgtgctc ctgtcgttga ggacccggct aggctggcgg 1501 ggttgcctta ctggttagca gaatgaatca ccgatacgcg agcgaacgtg aagcgactgc 1561 tgctgcaaaa cgtctgcgac ctgagcaaca acatgaatgg tcttcggttt ccgtgtttcg 1621 taaagtctgg aaacgcggaa gtcagcgccc tgcaccatta tgttccggat ctgcatcgca 1681 ggatgctgct ggctaccctg tggaacacct acatctgtat taacgaagcg ctggcattga 1741 ccctgagtga tttttctctg gtcccgccgc atccataccg ccagttgttt accctcacaa 1801 cgttccagta accgggcatg ttcatcatca gtaacccgta tcgtgagcat cctctctcgt 1861 ttcatcggta tcattacccc catgaacaga aatccccctt acacggaggc atcagtgacc 1921 aaacaggaaa aaaccgccct taacatggcc cgctttatca gaagccagac attaacgctt 1981 ctggagaaac tcaacgagct ggacgcggat gaacaggcag acatctgtga atcgcttcac 2041 gaccacgctg atgagcttta ccgcagggcc gcgatcaagc aggtgcgaca gacgtcatac 2101 tagatatcaa gcgacttctc ctatcccctg ggaacacatc aatctcaccg gagaatatcg 2161 ctggccaaag ccttagcgta ggattccgcc ccttcccgca aacgacccca aacaggaaac 2221 gcagctgaaa cgggaagctc aacacccact gacgcatggg ttgttcaggc agtacttcat 2281 caaccagcaa ggcggcactt tcggccatcc gccgcgcccc acagctcggg cagaaaccgc 2341 gacgcttaca gctgaaagcg accaggtgct cggcgtggca agactcgcag cgaacccgta 2401 gaaagccatg ctccagccgc ccgcattgga gaaattcttc aaattcccgt tgcacatagc 2461 ccggcaattc ctttccctgc tctgccataa gcgcagcgaa tgccgggtaa tactcgtcaa 2521 cgatctgata gagaagggtt tgctcgggtc ggtggctctg gtaacgacca gtatcccgat 2581 cccggctggc cgtcctggcc gccacatgag gcatgttccg cgtccttgca atactgtgtt 2641 tacatacagt ctatcgctta gcggaaagtt cttttaccct cagccgaaat gcctgccgtt 2701 gctagacatt gccagccagt gcccgtcact cccgtactaa ctgtcacgaa cccctgcaat 2761 aactgtcacg cccccctgca ataactgtca cgaacccctg caataactgt cacgccccca 2821 aacctgcaaa cccagcaggg gcgggggctg gcggggtgtt ggaaaaatcc atccatgatt 2881 atctaagaat aatccactag gcgcggttat cagcgccctt gtggggcgct gctgcccttg 2941 cccaatatgc ccggccagag gccggatagc tggtctattc gctgcgctag gctacacacc 3001 gccccaccgc tgcgcggcag ggggaaaggc gggcaaagcc cgctaaaccc cacaccaaac 3061 cccgcagaaa tacgctggag cgcttttagc cgctttagcg gcctttcccc ctacccgaag 3121 ggtgggggcg cgtgtgcagc cccgcagggc ctgtctcggt cgatcattca gcccggctca 3181 tccttctggc gtggcggcag accgaacaag gcgcggtcgt ggtcgcgttc aaggtacgca 3241 tccattgccg ccatgagccg atcctccggc cactcgctgc tgttcacctt ggccaaaatc 3301 atggccccca ccagcacctt gcgccttgtt tcgttcttgc gctcttgctg ctgttccctt 3361 gcccgcaccc gctgaatttc ggcattgatt cgcgctcgtt gttcttcgag cttggccagc 3421 cgatccgccg ccttgttgct ccccttaacc atcttgacac cccattgtta atgtgctgtc 3481 tcgtaggcta tcatggaggc acagcggcgg caatcccgac cctactttgt aggggagggc 3541 gcacttaccg gtttctcttc gagaaactgg cctaacggcc acccttcggg cggtgcgctc 3601 tccgagggcc attgcatgga gccgaaaagc aaaagcaaca gcgaggcagc atggcgattt 3661 atcaccttac ggcgaaaacc ggcagcaggt cgggcggcca atcggccagg gccaaggccg 3721 actacatcca gcgcgaaggc aagtatgccc gcgacatgga tgaagtcttg cacgccgaat 3781 ccgggcacat gccggagttc gtcgagcggc ccgccgacta ctgggatgct gccgacctgt 3841 atgaacgcgc caatgggcgg ctgttcaagg aggtcgaatt tgccctgccg gtcgagctga 3901 ccctcgacca gcagaaggcg ctggcgtccg agttcgccca gcacctgacc ggtgccgagc 3961 gcctgccgta tacgctggcc atccatgccg gtggcggcga gaacccgcac tgccacctga 4021 tgatctccga gcggatcaat gacggcatcg agcggcccgc cgctcagtgg ttcaagcggt 4081 acaacggcaa gaccccggag aagggcgggg cacagaagac cgaagcgctc aagcccaagg 4141 catggcttga gcagacccgc gaggcatggg ccgaccatgc caaccgggca ttagagcggg 4201 ctggccacga cgcccgcatt gaccacagaa cacttgaggc gcagggcatc gagcgcctgc 4261 ccggtgttca cctggggccg aacgtggtgg agatggaagg ccggggcatc cgcaccgacc 4321 gggcagacgt ggccctgaac atcgacaccg ccaacgccca gatcatcgac ttacaggaat 4381 accgggaggc aatagaccat gaacgcaatc gacagagtga agaaatccag aggcatcaac 4441 gagttagcgg agcagatcga accgctggcc cagagcatgg cgacactggc cgacgaagcc 4501 cggcaggtca tgagccagac ccagcaggcc agcgaggcgc aggcggcgga gtggctgaaa 4561 gcccagcgcc agacaggggc ggcatgggtg gagctggcca aagagttgcg ggaggtagcc 4621 gccgaggtga gcagcgccgc gcagagcgcc cggagcgcgt cgcgggggtg gcactggaag 4681 ctatggctaa ccgtgatgct ggcttccatg atgcctacgg tggtgctgct gatcgcatcg 4741 ttgctcttgc tcgacctgac gccactgaca accgaggacg gctcgatctg gctgcgcttg 4801 gtggcccgat gaagaacgac aggactttgc aggccatagg ccgacagctc aaggccatgg 4861 gctgtgagcg cttcgatatc ggcgtcaggg acgccaccac cggccagatg atgaaccggg 4921 aatggtcagc cgccgaagtg ctccagaaca cgccatggct caagcggatg aatgcccagg 4981 gcaatgacgt gtatatcagg cccgccgagc aggagcggca tggtctggtg ctggtggacg 5041 acctcagcga gtttgacctg gatgacatga aagccgaggg ccgggagcct gccctggtag 5101 tggaaaccag cccgaagaac tatcaggcat gggtcaaggt ggccgacgcc gcaggcggtg 5161 aacttcgggg gcagattgcc cggacgctgg ccagcgagta cgacgccgac ccggccagcg 5221 ccgacagccg ccactatggc cgcttggcgg gcttcaccaa ccgcaaggac aagcacacca 5281 cccgcgccgg ttatcagccg tgggtgctgc tgcgtgaatc caagggcaag accgccaccg 5341 ctggcccggc gctggtgcag caggctggcc agcagatcga gcaggcccag cggcagcagg 5401 agaaggcccg caggctggcc agcctcgaac tgcccgagcg gcagcttagc cgccaccggc 5461 gcacggcgct ggacgagtac cgcagcgaga tggccgggct ggtcaagcgc ttcggtgatg 5521 acctcagcaa gtgcgacttt atcgccgcgc agaagctggc cagccggggc cgcagtgccg 5581 aggaaatcgg caaggccatg gccgaggcca gcccagcgct ggcagagcgc aagcccggcc 5641 acgaagcgga ttacatcgag cgcaccgtca gcaaggtcat gggtctgccc agcgtccagc 5701 ttgcgcgggc cgagctggca cgggcaccgg caccccgcca gcgaggcatg gacaggggcg 5761 ggccagattt cagcatgtag tgcttgcgtt ggtactcacg cctgttatac tatgagtact 5821 cacgcacaga agggggtttt atggaatacg aaaaaagcgc ttcagggtcg gtctacctga 5881 tcaaaagtga caagggctat tggttgcccg gtggctttgg ttatacgtca aacaaggccg 5941 aggctggccg cttttcagtc gctgatatgg ccagccttaa ccttgacggc tgcaccttgt 6001 ccttgttccg cgaagacaag cctttcggcc ccggcaagtt tctcggtgac tgatatgaaa 6061 gaccaaaagg acaagcagac cggcgacctg ctggccagcc ctgacgctgt acgccaagcg 6121 cgatatgccg agcgcatgaa ggccaaaggg atgcgtcagc gcaagttctg gctgaccgac 6181 gacgaatacg aggcgctgcg cgagtgcctg gaagaactca gagcggcgca gggcgggggt 6241 agtgaccccg ccagcgccta accaccaact gcctgcaaag gaggcaatca atggctaccc 6301 ataagcctat caatattctg gaggcgttcg cagcagcgcc gccaccgctg gactacgttt 6361 tgcccaacat ggtggccggt acggtcgggg cgctggtgtc gcccggtggt gccggtaaat 6421 ccatgctggc cctgcaactg gccgcacaga ttgcaggcgg gccggatctg ctggaggtgg 6481 gcgaactgcc caccggcccg gtgatctacc tgcccgccga agacccgccc accgccattc 6541 atcaccgcct gcacgccctt ggggcgcacc tcagcgccga ggaacggcaa gccgtggctg 6601 acggcctgct gatccagccg ctgatcggca gcctgcccaa catcatggcc ccggagtggt 6661 tcgacggcct caagcgcgcc gccgagggcc gccgcctgat ggtgctggac acgctgcgcc 6721 ggttccacat cgaggaagaa aacgccagcg gccccatggc ccaggtcatc ggtcgcatgg 6781 aggccatcgc cgccgatacc gggtgctcta tcgtgttcct gcaccatgcc agcaagggcg 6841 cggccatgat gggcgcaggc gaccagcagc aggccagccg gggcagctcg gtactggtcg 6901 ataacatccg ctggcagtcc tacctgtcga gcatgaccag cgccgaggcc gaggaatggg 6961 gtgtggacga cgaccagcgc cggttcttcg tccgcttcgg tgtgagcaag gccaactatg 7021 gcgcaccgtt cgctgatcgg tggttcaggc ggcatgacgg cggggtgctc aagcccgccg 7081 tgctggagag gcagcgcaag agcaaggggg tgccccgtgg tgaagcctaa gaacaagcac 7141 agcctcagcc acgtccggca cgacccggcg cactgtctgg cccccggcct gttccgtgcc 7201 ctcaagcggg gcgagcgcaa gcgcagcaag ctggacgtga cgtatgacta cggcgacggc 7261 aagcggatcg agttcagcgg cccggagccg ctgggcgctg atgatctgcg catcctgcaa 7321 gggctggtgg ccatggctgg gcctaatggc ctagtgcttg gcccggaacc caagaccgaa 7381 ggcggacggc agctccggct gttcctggaa cccaagtggg aggccgtcac cgctgaatgc 7441 catgtggtca aaggtagcta tcgggcgctg gcaaaggaaa tcggggcaga ggtcgatagt 7501 ggtggggcgc tcaagcacat acaggactgc atcgagcgcc tttggaaggt atccatcatc 7561 gcccagaatg gccgcaagcg gcaggggttt cggctgctgt cggagtacgc cagcgacgag 7621 gcggacgggc gcctgtacgt ggccctgaac cccttgatcg cgcaggccgt catgggtggc 7681 ggccagcatg tgcgcatcag catggacgag gtgcgggcgc tggacagcga aaccgcccgc 7741 ctgctgcacc agcggctgtg tggctggatc gaccccggca aaaccggcaa ggcttccata 7801 gataccttgt gcggctatgt ctggccgtca gaggccagtg gttcgaccat gcgcaagcgc 7861 cgccagcggg tgcgcgaggc gttgccggag ctggtcgcgc tgggctggac ggtaaccgag 7921 ttcgcggcgg gcaagtacga catcacccgg cccaaggcgg caggctgacc ccccccactc 7981 tattgtaaac aagacatttt tatcttttat attcaatggc ttattttcct gctaattggt 8041 aataccatga aaaataccat gctcagaaaa ggcttaacaa tattttgaaa aattgcctac 8101 tgagcgctgc cgcacagctc cataggccgc tttcctggct ttgcttccag atgtatgctc 8161 ttctgctcct cgagcaagac gtttcccgtt gaatatggct cataacaccc cttgtattac 8221 tgtttatgta agcagacagt tttattgttc atgatgatat atttttatct tgtgcaatgt 8281 aacatcagag attttgagac acaacgtggc tttgttgaat aaatcgaact tttgctgagt 8341 tgaaggatca gatcacgcat cttcccgaca acgcagaccg ttccgtggca aagcaaaagt 8401 tcaaaatcac caactggtcc acctacaaca aagctctcat caaccgtggc tccctcactt 8461 tctggctgga tgatggggcg attcaggcct ggtatgagtc agcaacacct tcttcacgag 8521 gcagacctca gcgctaaccg tttttatcag gctctgggag gcagaataaa tgatcatatc 8581 gtcaattatt acctccacgg ggagagcctg agcaaactgg cctcaggcat ttgagaagca 8641 cacggtcaca ctgcttccgg tagtcaataa accggtaaac cagcaataga cataagcggc 8701 tatttaacga ccctgccctg aaccgacgac cgggtcgaat ttgctttcga atttctgcca 8761 ttcatccgct tattatcact tattcaggcg tagcaccagg cgtttaaggg caccaataac 8821 tgccttaaaa aaattacgcc ccgccctgcc actcatcgca gtactgttgt aattcattaa 8881 gcattctgcc gacatggaag ccatcacaga cggcatgatg aacctgaatc gccagcggca 8941 tcagcacctt gtcgccttgc gtataatatt tgcccatggt gaaaacgggg gcgaagaagt 9001 tgtccatatt ggccacgttt aaatcaaaac tggtgaaact cacccaggga ttggctgaga 9061 cgaaaaacat attctcaata aaccctttag ggaaataggc caggttttca ccgtaacacg 9121 ccacatcttg cgaatatatg tgtagaaact gccggaaatc gtcgtggtat tcactccaga 9181 gcgatgaaaa cgtttcagtt tgctcatgga aaacggtgta acaagggtga acactatccc 9241 atatcaccag ctcaccgtct ttcattgcca tacggaa //