LOCUS LT727446 12950 bp DNA circular SYN 06-FEB-2017
DEFINITION Cloning vector pKT240tet1, complete sequence.
ACCESSION LT727446
VERSION LT727446.1
KEYWORDS .
SOURCE Cloning vector pKT240tet1
ORGANISM Cloning vector pKT240tet1
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 12950)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..12950
/organism="Cloning vector pKT240tet1"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 3162."
/db_xref="taxon:1944717"
CDS 86..1276
/transl_table=11
/note="tetracycline resistance"
/protein_id="SJL88476.1"
/translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASH
YGVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAG
RIVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLH
APFLAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTV
FFIMQLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGE
KQAIIAGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQG
QLQGSLAALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSR
ATST"
rep_origin 2748..3143
/note="RSF1010 ori"
CDS complement(3168..3452)
/transl_table=11
/note="mobC"
/protein_id="SJL88477.1"
/translation="MVKGSNKAADRLAKLEEQRARINAEIQRVRAREQQQERKNETRR
KVLVGAMILAKVNSSEWPEDRLMAAMDAYLERDHDRALFGLPPRQKDEPG"
rep_origin 3483..3570
/note="RSF1010 oriT"
CDS 3651..5780
/transl_table=11
/note="mobA"
/protein_id="SJL88478.1"
/translation="MAIYHLTAKTGSRSGGQSARAKADYIQREGKYARDMDEVLHAES
GHMPEFVERPADYWDAADLYERANGRLFKEVEFALPVELTLDQQKALASEFAQHLTGA
ERLPYTLAIHAGGGENPHCHLMISERINDGIERPAAQWFKRYNGKTPEKGGAQKTEAL
KPKAWLEQTREAWADHANRALERAGHDARIDHRTLEAQGIERLPGVHLGPNVVEMEGR
GIRTDRADVALNIDTANAQIIDLQEYREAIDHERNRQSEEIQRHQRVSGADRTAGPEH
GDTGRRSPAGHEPDPAGQRGAGGGVAESPAPDRGGMGGAGQRVAGGSRRGEQRRAERP
ERVAGVALEAMANRDAGFHDAYGGAADRIVALARPDATDNRGRLDLAALGGPMKNDRT
LQAIGRQLKAMGCERFDIGVRDATTGQMMNREWSAAEVLQNTPWLKRMNAQGNDVYIR
PAEQERHGLVLVDDLSEFDLDDMKAEGREPALVVETSPKNYQAWVKVADAAGGELRGQ
IARTLASEYDADPASADSRHYGRLAGFTNRKDKHTTRAGYQPWVLLRESKGKTATAGP
ALVQQAGQQIEQAQRQQEKARRLASLELPERQLSRHRRTALDEYRSEMAGLVKRFGDD
LSKCDFIAAQKLASRGRSAEEIGKAMAEASPALAERKPGHEADYIERTVSKVMGLPSV
QLARAELARAPAPRQRGMDRGGPDFSM"
CDS 4399..4812
/transl_table=11
/note="mobB"
/protein_id="SJL88479.1"
/translation="MNAIDRVKKSRGINELAEQIEPLAQSMATLADEARQVMSQTQQA
SEAQAAEWLKAQRQTGAAWVELAKELREVAAEVSSAAQSARSASRGWHWKLWLTVMLA
SMMPTVVLLIASLLLLDLTPLTTEDGSIWLRLVAR"
CDS 4809..5780
/transl_table=11
/note="repB"
/protein_id="SJL88480.1"
/translation="MKNDRTLQAIGRQLKAMGCERFDIGVRDATTGQMMNREWSAAEV
LQNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDLSEFDLDDMKAEGREPALVVETSP
KNYQAWVKVADAAGGELRGQIARTLASEYDADPASADSRHYGRLAGFTNRKDKHTTRA
GYQPWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQEKARRLASLELPERQLSRHRR
TALDEYRSEMAGLVKRFGDDLSKCDFIAAQKLASRGRSAEEIGKAMAEASPALAERKP
GHEADYIERTVSKVMGLPSVQLARAELARAPAPRQRGMDRGGPDFSM"
CDS 6055..6261
/transl_table=11
/note="rpsF"
/protein_id="SJL88481.1"
/translation="MKDQKDKQTGDLLASPDAVRQARYAERMKAKGMRQRKFWLTDDE
YEALRECLEELRAAQGGGSDPASA"
CDS 6291..7130
/transl_table=11
/note="repA"
/protein_id="SJL88482.1"
/translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKS
MLALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAV
ADGLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVI
GRMEAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSA
EAEEWGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPR
GEA"
CDS 7117..7968
/transl_table=11
/note="repC"
/protein_id="SJL88483.1"
/translation="MVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYD
YGDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWE
AVTAECHVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRL
LSEYASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWI
DPGKTGKASIDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDI
TRPKAAG"
misc_feature 7914..8969
/note="RSF'"
CDS complement(9501..10361)
/transl_table=11
/note="ampicillin resistance"
/protein_id="SJL88484.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
CDS complement(11061..11876)
/transl_table=11
/note="Tn903 neomycin resistance"
/protein_id="SJL88485.1"
/translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLY
GKPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIP
GKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGL
VDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVG
RVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
BASE COUNT 2879 a 3777 c 3583 g 2702 t
ORIGIN
1 ttctcatgtt tgacagctta tcatcgataa gctttaatgc ggtagtttat cacagttaaa
61 ttgctaacgc agtcaggcac cgtgtatgaa atctaacaat gcgctcatcg tcatcctcgg
121 caccgtcacc ctggatgctg taggcatagg cttggttatg ccggtactgc cgggcctctt
181 gcgggatatc gtccattccg acagcatcgc cagtcactat ggcgtgctgc tagcgctata
241 tgcgttgatg caatttctat gcgcacccgt tctcggagca ctgtccgacc gctttggccg
301 ccgcccagtc ctgctcgctt cgctacttgg agccactatc gactacgcga tcatggcgac
361 cacacccgtc ctgtggatcc tctacgccgg acgcatcgtg gccggcatca ccggcgccac
421 aggtgcggtt gctggcgcct atatcgccga catcaccgat ggggaagatc gggctcgcca
481 cttcgggctc atgagcgctt gtttcggcgt gggtatggtg gcaggccccg tggccggggg
541 actgttgggc gccatctcct tgcatgcacc attccttgcg gcggcggtgc tcaacggcct
601 caacctacta ctgggctgct tcctaatgca ggagtcgcat aagggagagc gtcgaccgat
661 gcccttgaga gccttcaacc cagtcagctc cttccggtgg gcgcggggca tgactatcgt
721 cgccgcactt atgactgtct tctttatcat gcaactcgta ggacaggtgc cggcagcgct
781 ctgggtcatt ttcggcgagg accgctttcg ctggagcgcg acgatgatcg gcctgtcgct
841 tgcggtattc ggaatcttgc acgccctcgc tcaagccttc gtcactggtc ccgccaccaa
901 acgtttcggc gagaagcagg ccattatcgc cggcatggcg gccgacgcgc tgggctacgt
961 cttgctggcg ttcgcgacgc gaggctggat ggccttcccc attatgattc ttctcgcttc
1021 cggcggcatc gggatgcccg cgttgcaggc catgctgtcc aggcaggtag atgacgacca
1081 tcagggacag cttcaaggat cgctcgcggc tcttaccagc ctaacttcga tcactggacc
1141 gctgatcgtc acggcgattt atgccgcctc ggcgagcaca tggaacgggt tggcatggat
1201 tgtaggcgcc gccctatacc ttgtctgcct ccccgcgttg cgtcgcggtg catggagccg
1261 ggccacctcg acctgaatgg aagccggcgg cacctcgcta acggattcac cactccaaga
1321 attggagcca atcaattctt gcggagaact gtgaatgcgc aaaccaaccc ttggcagaac
1381 atatccatcg cgtccgccat ctccagcagc cgcacgcggc gcatctcggg cagcgttggg
1441 tcctggccac gggtgcgcat gatcgtgctc ctgtcgttga ggacccggct aggctggcgg
1501 ggttgcctta ctggttagca gaatgaatca ccgatacgcg agcgaacgtg aagcgactgc
1561 tgctgcaaaa cgtctgcgac ctgagcaaca acatgaatgg tcttcggttt ccgtgtttcg
1621 taaagtctgg aaacgcggaa gtcagcgccc tgcaccatta tgttccggat ctgcatcgca
1681 ggatgctgct ggctaccctg tggaacacct acatctgtat taacgaagcg ctggcattga
1741 ccctgagtga tttttctctg gtcccgccgc atccataccg ccagttgttt accctcacaa
1801 cgttccagta accgggcatg ttcatcatca gtaacccgta tcgtgagcat cctctctcgt
1861 ttcatcggta tcattacccc catgaacaga aatccccctt acacggaggc atcagtgacc
1921 aaacaggaaa aaaccgccct taacatggcc cgctttatca gaagccagac attaacgctt
1981 ctggagaaac tcaacgagct ggacgcggat gaacaggcag acatctgtga atcgcttcac
2041 gaccacgctg atgagcttta ccgcagggcc gcgatcaagc aggtgcgaca gacgtcatac
2101 tagatatcaa gcgacttctc ctatcccctg ggaacacatc aatctcaccg gagaatatcg
2161 ctggccaaag ccttagcgta ggattccgcc ccttcccgca aacgacccca aacaggaaac
2221 gcagctgaaa cgggaagctc aacacccact gacgcatggg ttgttcaggc agtacttcat
2281 caaccagcaa ggcggcactt tcggccatcc gccgcgcccc acagctcggg cagaaaccgc
2341 gacgcttaca gctgaaagcg accaggtgct cggcgtggca agactcgcag cgaacccgta
2401 gaaagccatg ctccagccgc ccgcattgga gaaattcttc aaattcccgt tgcacatagc
2461 ccggcaattc ctttccctgc tctgccataa gcgcagcgaa tgccgggtaa tactcgtcaa
2521 cgatctgata gagaagggtt tgctcgggtc ggtggctctg gtaacgacca gtatcccgat
2581 cccggctggc cgtcctggcc gccacatgag gcatgttccg cgtccttgca atactgtgtt
2641 tacatacagt ctatcgctta gcggaaagtt cttttaccct cagccgaaat gcctgccgtt
2701 gctagacatt gccagccagt gcccgtcact cccgtactaa ctgtcacgaa cccctgcaat
2761 aactgtcacg cccccctgca ataactgtca cgaacccctg caataactgt cacgccccca
2821 aacctgcaaa cccagcaggg gcgggggctg gcggggtgtt ggaaaaatcc atccatgatt
2881 atctaagaat aatccactag gcgcggttat cagcgccctt gtggggcgct gctgcccttg
2941 cccaatatgc ccggccagag gccggatagc tggtctattc gctgcgctag gctacacacc
3001 gccccaccgc tgcgcggcag ggggaaaggc gggcaaagcc cgctaaaccc cacaccaaac
3061 cccgcagaaa tacgctggag cgcttttagc cgctttagcg gcctttcccc ctacccgaag
3121 ggtgggggcg cgtgtgcagc cccgcagggc ctgtctcggt cgatcattca gcccggctca
3181 tccttctggc gtggcggcag accgaacaag gcgcggtcgt ggtcgcgttc aaggtacgca
3241 tccattgccg ccatgagccg atcctccggc cactcgctgc tgttcacctt ggccaaaatc
3301 atggccccca ccagcacctt gcgccttgtt tcgttcttgc gctcttgctg ctgttccctt
3361 gcccgcaccc gctgaatttc ggcattgatt cgcgctcgtt gttcttcgag cttggccagc
3421 cgatccgccg ccttgttgct ccccttaacc atcttgacac cccattgtta atgtgctgtc
3481 tcgtaggcta tcatggaggc acagcggcgg caatcccgac cctactttgt aggggagggc
3541 gcacttaccg gtttctcttc gagaaactgg cctaacggcc acccttcggg cggtgcgctc
3601 tccgagggcc attgcatgga gccgaaaagc aaaagcaaca gcgaggcagc atggcgattt
3661 atcaccttac ggcgaaaacc ggcagcaggt cgggcggcca atcggccagg gccaaggccg
3721 actacatcca gcgcgaaggc aagtatgccc gcgacatgga tgaagtcttg cacgccgaat
3781 ccgggcacat gccggagttc gtcgagcggc ccgccgacta ctgggatgct gccgacctgt
3841 atgaacgcgc caatgggcgg ctgttcaagg aggtcgaatt tgccctgccg gtcgagctga
3901 ccctcgacca gcagaaggcg ctggcgtccg agttcgccca gcacctgacc ggtgccgagc
3961 gcctgccgta tacgctggcc atccatgccg gtggcggcga gaacccgcac tgccacctga
4021 tgatctccga gcggatcaat gacggcatcg agcggcccgc cgctcagtgg ttcaagcggt
4081 acaacggcaa gaccccggag aagggcgggg cacagaagac cgaagcgctc aagcccaagg
4141 catggcttga gcagacccgc gaggcatggg ccgaccatgc caaccgggca ttagagcggg
4201 ctggccacga cgcccgcatt gaccacagaa cacttgaggc gcagggcatc gagcgcctgc
4261 ccggtgttca cctggggccg aacgtggtgg agatggaagg ccggggcatc cgcaccgacc
4321 gggcagacgt ggccctgaac atcgacaccg ccaacgccca gatcatcgac ttacaggaat
4381 accgggaggc aatagaccat gaacgcaatc gacagagtga agaaatccag aggcatcaac
4441 gagttagcgg agcagatcga accgctggcc cagagcatgg cgacactggc cgacgaagcc
4501 cggcaggtca tgagccagac ccagcaggcc agcgaggcgc aggcggcgga gtggctgaaa
4561 gcccagcgcc agacaggggc ggcatgggtg gagctggcca aagagttgcg ggaggtagcc
4621 gccgaggtga gcagcgccgc gcagagcgcc cggagcgcgt cgcgggggtg gcactggaag
4681 ctatggctaa ccgtgatgct ggcttccatg atgcctacgg tggtgctgct gatcgcatcg
4741 ttgctcttgc tcgacctgac gccactgaca accgaggacg gctcgatctg gctgcgcttg
4801 gtggcccgat gaagaacgac aggactttgc aggccatagg ccgacagctc aaggccatgg
4861 gctgtgagcg cttcgatatc ggcgtcaggg acgccaccac cggccagatg atgaaccggg
4921 aatggtcagc cgccgaagtg ctccagaaca cgccatggct caagcggatg aatgcccagg
4981 gcaatgacgt gtatatcagg cccgccgagc aggagcggca tggtctggtg ctggtggacg
5041 acctcagcga gtttgacctg gatgacatga aagccgaggg ccgggagcct gccctggtag
5101 tggaaaccag cccgaagaac tatcaggcat gggtcaaggt ggccgacgcc gcaggcggtg
5161 aacttcgggg gcagattgcc cggacgctgg ccagcgagta cgacgccgac ccggccagcg
5221 ccgacagccg ccactatggc cgcttggcgg gcttcaccaa ccgcaaggac aagcacacca
5281 cccgcgccgg ttatcagccg tgggtgctgc tgcgtgaatc caagggcaag accgccaccg
5341 ctggcccggc gctggtgcag caggctggcc agcagatcga gcaggcccag cggcagcagg
5401 agaaggcccg caggctggcc agcctcgaac tgcccgagcg gcagcttagc cgccaccggc
5461 gcacggcgct ggacgagtac cgcagcgaga tggccgggct ggtcaagcgc ttcggtgatg
5521 acctcagcaa gtgcgacttt atcgccgcgc agaagctggc cagccggggc cgcagtgccg
5581 aggaaatcgg caaggccatg gccgaggcca gcccagcgct ggcagagcgc aagcccggcc
5641 acgaagcgga ttacatcgag cgcaccgtca gcaaggtcat gggtctgccc agcgtccagc
5701 ttgcgcgggc cgagctggca cgggcaccgg caccccgcca gcgaggcatg gacaggggcg
5761 ggccagattt cagcatgtag tgcttgcgtt ggtactcacg cctgttatac tatgagtact
5821 cacgcacaga agggggtttt atggaatacg aaaaaagcgc ttcagggtcg gtctacctga
5881 tcaaaagtga caagggctat tggttgcccg gtggctttgg ttatacgtca aacaaggccg
5941 aggctggccg cttttcagtc gctgatatgg ccagccttaa ccttgacggc tgcaccttgt
6001 ccttgttccg cgaagacaag cctttcggcc ccggcaagtt tctcggtgac tgatatgaaa
6061 gaccaaaagg acaagcagac cggcgacctg ctggccagcc ctgacgctgt acgccaagcg
6121 cgatatgccg agcgcatgaa ggccaaaggg atgcgtcagc gcaagttctg gctgaccgac
6181 gacgaatacg aggcgctgcg cgagtgcctg gaagaactca gagcggcgca gggcgggggt
6241 agtgaccccg ccagcgccta accaccaact gcctgcaaag gaggcaatca atggctaccc
6301 ataagcctat caatattctg gaggcgttcg cagcagcgcc gccaccgctg gactacgttt
6361 tgcccaacat ggtggccggt acggtcgggg cgctggtgtc gcccggtggt gccggtaaat
6421 ccatgctggc cctgcaactg gccgcacaga ttgcaggcgg gccggatctg ctggaggtgg
6481 gcgaactgcc caccggcccg gtgatctacc tgcccgccga agacccgccc accgccattc
6541 atcaccgcct gcacgccctt ggggcgcacc tcagcgccga ggaacggcaa gccgtggctg
6601 acggcctgct gatccagccg ctgatcggca gcctgcccaa catcatggcc ccggagtggt
6661 tcgacggcct caagcgcgcc gccgagggcc gccgcctgat ggtgctggac acgctgcgcc
6721 ggttccacat cgaggaagaa aacgccagcg gccccatggc ccaggtcatc ggtcgcatgg
6781 aggccatcgc cgccgatacc gggtgctcta tcgtgttcct gcaccatgcc agcaagggcg
6841 cggccatgat gggcgcaggc gaccagcagc aggccagccg gggcagctcg gtactggtcg
6901 ataacatccg ctggcagtcc tacctgtcga gcatgaccag cgccgaggcc gaggaatggg
6961 gtgtggacga cgaccagcgc cggttcttcg tccgcttcgg tgtgagcaag gccaactatg
7021 gcgcaccgtt cgctgatcgg tggttcaggc ggcatgacgg cggggtgctc aagcccgccg
7081 tgctggagag gcagcgcaag agcaaggggg tgccccgtgg tgaagcctaa gaacaagcac
7141 agcctcagcc acgtccggca cgacccggcg cactgtctgg cccccggcct gttccgtgcc
7201 ctcaagcggg gcgagcgcaa gcgcagcaag ctggacgtga cgtatgacta cggcgacggc
7261 aagcggatcg agttcagcgg cccggagccg ctgggcgctg atgatctgcg catcctgcaa
7321 gggctggtgg ccatggctgg gcctaatggc ctagtgcttg gcccggaacc caagaccgaa
7381 ggcggacggc agctccggct gttcctggaa cccaagtggg aggccgtcac cgctgaatgc
7441 catgtggtca aaggtagcta tcgggcgctg gcaaaggaaa tcggggcaga ggtcgatagt
7501 ggtggggcgc tcaagcacat acaggactgc atcgagcgcc tttggaaggt atccatcatc
7561 gcccagaatg gccgcaagcg gcaggggttt cggctgctgt cggagtacgc cagcgacgag
7621 gcggacgggc gcctgtacgt ggccctgaac cccttgatcg cgcaggccgt catgggtggc
7681 ggccagcatg tgcgcatcag catggacgag gtgcgggcgc tggacagcga aaccgcccgc
7741 ctgctgcacc agcggctgtg tggctggatc gaccccggca aaaccggcaa ggcttccata
7801 gataccttgt gcggctatgt ctggccgtca gaggccagtg gttcgaccat gcgcaagcgc
7861 cgccagcggg tgcgcgaggc gttgccggag ctggtcgcgc tgggctggac ggtaaccgag
7921 ttcgcggcgg gcaagtacga catcacccgg cccaaggcgg caggctgacc ccccccactc
7981 tattgtaaac aagacatttt tatcttttat attcaatggc ttattttcct gctaattggt
8041 aataccatga aaaataccat gctcagaaaa ggcttaacaa tattttgaaa aattgcctac
8101 tgagcgctgc cgcacagctc cataggccgc tttcctggct ttgcttccag atgtatgctc
8161 ttctgctcct gcagctaatg gatcaccgca aacaggttac tcgcctgggg attccctttc
8221 gacccgagca tccgtatgat actcatgctc gattattatt attatagaag cccccatgaa
8281 taaatcgctc atcattttcg gcatcgtcaa cataacctcg gacagtttct ccgatggagg
8341 ccggtatctg gcgccagacg cagccattgc gcaggcgcgt aagctgatgg ccgagggggc
8401 agatgtgatc gacctggtcc ggcatccagc aatcccgacg ccgcgcctgt ttcgtccgac
8461 acagaaatcg cgcgtatgcg ccggtgctgg acgcgctcag gcagatggca ttcccgtctc
8521 gctcgacagt tatcaacccg cgacgcaagc ctatgccttg tcgcgtggtg tggcctatct
8581 caatgatatt cgcggttttc cagacgctgc gttctatccg caattggcga aatcatctgc
8641 caaactcgtc gttatgcatt cggtgcaaga cgggcaggca gatcggcgcg aggcacccgc
8701 tggcgacatc atggatcaca ttgcggcgtt ctttgacgcg cgcatcgcgg cgctgacggg
8761 tgccggtatc aaacgcaacc gccttgtcct tgatcccggc atggggtttt ttctgggggc
8821 tgctcccgaa acctcgctct cggtgctggc gcggttcgat gaattgcggc tgcgcttcga
8881 tttgccggtg cttctgtctg tttcgcgcaa atcctttctg cgcgcgctca caggccgtgg
8941 tccgggggtg tcggggccgc gacactcgcn nnnncggaag accactgccc gctgttcacc
9001 ttacgggctg tctgattcag gttatttccg atggcggcca gctgacgcag taacggcggt
9061 gccagtgtcg gcagttttcc ggaacgggca accggctccc ccaggcagac ccgccgcatc
9121 cataccgcca gttgtttacc ctcacagcgt tcaagtaacc gggcatgttc atcatcagta
9181 acccgtattg tgagcatcct ctcgcgtttc atcggtatca ttaccccatg aacagaaatc
9241 ccccttacac ggaggcatca gtgactaaac aggaaaaaac cgcccttaac atggcccgct
9301 ttatcagaag ccagacatta acgctgctgg agaagctcaa cgaactggan nnnggggtct
9361 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
9421 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
9481 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
9541 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
9601 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
9661 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
9721 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
9781 ccagttaata gtttgcgcaa cgttgttgcc attgctgcag gcatcgtggt gtcacgctcg
9841 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
9901 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
9961 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
10021 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
10081 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaacac gggataatac cgcgccacat
10141 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
10201 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
10261 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
10321 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
10381 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
10441 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa
10501 gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt
10561 cttcaagaat tttataaacc gtggagcggg caatactgag ctgatgagca atttccgttg
10621 caccagtgcc cttctgatga agcgtcagca cgacgttcct gtccacggta cgcctgcggc
10681 caaatttgat tcctttcagc tttgcttcct gtcggccctc attcgtgcgc tctaggatcc
10741 tctacgccgg acgcatcgtg gccggcatca ccggcgctga ggtctgcctc gtgaagaagg
10801 tgttgctgac tcataccagg cctgaatcgc cccatcatcc agccagaaag tgagggagcc
10861 acggttgatg agagctttgt tgtaggtgga ccagttggtg attttgaact tttgctttgc
10921 cacggaacgg tctgcgttgt cgggaagatg cgtgatctga tccttcaact cagcaaaagt
10981 tcgatttatt caacaaagcc gccgtcccgt caagtcagcg taatgctctg ccagtgttac
11041 aaccaattaa ccaattctga ttagaaaaac tcatcgagca tcaaatgaaa ctgcaattta
11101 ttcatatcag gattatcaat accatatttt tgaaaaagcc gtttctgtaa tgaaggagaa
11161 aactcaccga ggcagttcca taggatggca agatcctggt atcggtctgc gattccgact
11221 cgtccaacat caatacaacc tattaatttc ccctcgtcaa aaataaggtt atcaagtgag
11281 aaatcaccat gagtgacgac tgaatccggt gagaatggca aaagcttatg catttctttc
11341 cagacttgtt caacaggcca gccattacgc tcgtcatcaa aatcactcgc atcaaccaaa
11401 ccgttattca ttcgtgattg cgcctgagcg agacgaaata cgcgatcgct gttaaaagga
11461 caattacaaa caggaatcga atgcaaccgg cgcaggaaca ctgccagcgc atcaacaata
11521 ttttcacctg aatcaggata ttcttctaat acctggaatg ctgttttccc ggggatcgca
11581 gtggtgagta accatgcatc atcaggagta cggataaaat gcttgatggt cggaagaggc
11641 ataaattccg tcagccagtt tagtctgacc atctcatctg taacatcatt ggcaacgcta
11701 cctttgccat gtttcagaaa caactctggc gcatcgggct tcccatacaa tcgatagatt
11761 gtcgcacctg attgcccgac attatcgcga gcccatttat acccatataa atcagcatcc
11821 atgttggaat ttaatcgcgg cctcgagcaa gacgtttccc gttgaatatg gctcataaca
11881 ccccttgtat tactgtttat gtaagcagac agttttattg ttcatgatga tatattttta
11941 tcttgtgcaa tgtaacatca gagattttga gacacaacgt ggctttgttg aataaatcga
12001 acttttgctg agttgaagga tcagatcacg catcttcccg acaacgcaga ccgttccgtg
12061 gcaaagcaaa agttcaaaat caccaactgg tccacctaca acaaagctct catcaaccgt
12121 ggctccctca ctttctggct ggatgatggg gcgattcagg cctggtatga gtcagcaaca
12181 ccttcttcac gaggcagacc tcagcgctaa ccgtttttat caggctctgg gaggcagaat
12241 aaatgatcat atcgtcaatt attacctcca cggggagagc ctgagcaaac tggcctcagg
12301 catttgagaa gcacacggtc acactgcttc cggtagtcaa taaaccggta aaccagcaat
12361 agacataagc ggctatttaa cgaccctgcc ctgaaccgac gaccgggtcg aatttgcttt
12421 cgaatttctg ccattcatcc gcttattatc acttattcag gcgtagcacc aggcgtttaa
12481 gggcaccaat aactgcctta aaaaaattac gccccgccct gccactcatc gcagtactgt
12541 tgtaattcat taagcattct gccgacatgg aagccatcac agacggcatg atgaacctga
12601 atcgccagcg gcatcagcac cttgtcgcct tgcgtataat atttgcccat ggtgaaaacg
12661 ggggcgaaga agttgtccat attggccacg tttaaatcaa aactggtgaa actcacccag
12721 ggattggctg agacgaaaaa catattctca ataaaccctt tagggaaata ggccaggttt
12781 tcaccgtaac acgccacatc ttgcgaatat atgtgtagaa actgccggaa atcgtcgtgg
12841 tattcactcc agagcgatga aaacgtttca gtttgctcat ggaaaacggt gtaacaaggg
12901 tgaacactat cccatatcac cagctcaccg tctttcattg ccatacggaa
//