LOCUS LT727445 6533 bp DNA circular SYN 06-FEB-2017
DEFINITION Mammalian expression vector pRcCMVhuIKB-alpha, complete sequence.
ACCESSION LT727445
VERSION LT727445.1
KEYWORDS .
SOURCE Mammalian expression vector pRcCMVhuIKB-alpha
ORGANISM Mammalian expression vector pRcCMVhuIKB-alpha
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 6533)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..6533
/organism="Mammalian expression vector pRcCMVhuIKB-alpha"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 3129."
/db_xref="taxon:1945055"
regulatory 232..818
/note="hCMV"
/regulatory_class="promoter"
regulatory 864..880
/note="T7"
/regulatory_class="promoter"
CDS 899..1852
/transl_table=11
/note="hMAD3"
/protein_id="SJL88473.1"
/translation="MFQAAERPQEWAMEGPRDGLKKERLLDDRHDSGLDSMKDEEYEQ
MVKELQEIRLEPQEVPRGSEPWKQQLTEDGDSFLHLAIIHEEKALTMEVIRQVKGDLA
FLNFQNNLQQTPLHLAVITNQPEIAEALLGAGCDPELRDFRGNTPLHLACEQGCLASV
GVLTQSCTTPHLHSILKATNYNGHTCLHLASIHGYLGIVELLVSLGADVNAQEPCNGR
TALHLAVDLQNPDLVSLLLKCGADVNRVTYQGYSPYQLTWGRPSTRIQQQLGQLTLEN
LQMLPESEDEESYDTESEFTEFTEDELPYDDCVFGGQRLTL"
regulatory complement(1992..2008)
/note="SP6"
/regulatory_class="promoter"
regulatory 2034..2258
/note="polyA"
/regulatory_class="terminator"
rep_origin 2304..2759
/note="f1 ori"
regulatory 2836..3118
/note="SV40 early promoter"
/regulatory_class="promoter"
rep_origin 3075..3150
/note="SV40 ori"
CDS 3242..4036
/transl_table=11
/note="neomycin resistance"
/protein_id="SJL88474.1"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY
QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
regulatory 4210..4284
/note="polyA"
/regulatory_class="terminator"
regulatory complement(4210..4284)
/note="polyA"
/regulatory_class="terminator"
rep_origin complement(4594..4996)
/note="pMB1 ori"
CDS complement(5537..6397)
/transl_table=11
/note="ampicillin resistance"
/protein_id="SJL88475.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
BASE COUNT 1500 a 1733 c 1699 g 1601 t
ORIGIN
1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg
61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt
481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
841 ctgcttaact ggcttatcga aattaatacg actcactata gggagaccca agcttgccat
901 gttccaggcg gccgagcgcc cccaggagtg ggccatggag ggcccccgcg acgggctgaa
961 gaaggagcgg ctactggacg accgccacga cagcggcctg gactccatga aagacgagga
1021 gtacgagcag atggtcaagg agctgcagga gatccgcctc gagccgcagg aggtgccgcg
1081 cggctcggag ccctggaagc agcagctcac cgaggacggg gactcgttcc tgcacttggc
1141 catcatccat gaagaaaagg cactgaccat ggaagtgatc cgccaggtga agggagacct
1201 ggctttcctc aacttccaga acaacctgca gcagactcca ctccacttgg ctgtgatcac
1261 caaccagcca gaaattgctg aggcacttct gggagctggc tgtgatcctg agctccgaga
1321 ctttcgagga aatacccccc tacaccttgc ctgtgagcag ggctgcctgg ccagcgtggg
1381 agtcctgact cagtcctgca ccaccccgca cctccactcc atcctgaagg ctaccaacta
1441 caatggccac acgtgtctac acttagcctc tatccatggc tacctgggca tcgtggagct
1501 tttggtgtcc ttgggtgctg atgtcaatgc tcaggagccc tgtaatggcc ggactgccct
1561 tcacctcgca gtggacctgc aaaatcctga cctggtgtca ctcctgttga agtgtggggc
1621 tgatgtcaac agagttacct accagggcta ttctccctac cagctcacct ggggccgccc
1681 aagcacccgg atacagcagc agctgggcca gctgacacta gaaaaccttc agatgctgcc
1741 agagagtgag gatgaggaga gctatgacac agagtcagag ttcacggagt tcacagagga
1801 cgagctgccc tatgatgact gtgtgtttgg aggccagcgt ctgacgttat gaggatccaa
1861 cccttctcac ctcggccgat aagcttggta ccgagctcgg atccactagt aacggccgcc
1921 agtgtgctgg aattctgcag atatccatca cactggcggc cgctcgagca tgcatctaga
1981 gggccctatt ctatagtgtc acctaaatgc tagagctcgc tgatcagcct cgactgtgcc
2041 ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg
2101 tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag
2161 gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga
2221 caatagcagg catgctgggg atgcggtggg ctctatggct tctgaggcgg aaagaaccag
2281 ctggggctcg aggggggatc cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt
2341 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc
2401 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg
2461 catcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta
2521 gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt
2581 ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat
2641 ctcggtctat tcttttgatt tataagggat tttggggatt tcggcctatt ggttaaaaaa
2701 tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgt ttacaattta
2761 aatatttgct tatacaatct tcctgttttt ggggcttttc tgattatcaa ccggggtggg
2821 taccgagctc gaattctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc
2881 ccaggcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa
2941 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
3001 catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc
3061 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc
3121 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct
3181 cccgggagct tggatatcca ttttcggatc tgatcaagag acaggatgag gatcgtttcg
3241 catgattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt
3301 cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc
3361 agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact
3421 gcaggacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt
3481 gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca
3541 ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat
3601 gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg
3661 catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga
3721 agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc gcatgcccga
3781 cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa
3841 tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga
3901 catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt
3961 cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct
4021 tgacgagttc ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc gacgcccaac
4081 ctgccatcac gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc
4141 gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc
4201 gcccacccca acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
4261 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
4321 aatgtatctt atcatgtctg gatcccgtcg acctcgagag cttggcgtaa tcatggtcat
4381 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa
4441 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc
4501 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc
4561 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact
4621 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
4681 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
4741 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
4801 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
4861 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
4921 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct caatgctcac
4981 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
5041 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
5101 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
5161 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga
5221 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
5281 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
5341 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
5401 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
5461 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
5521 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
5581 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
5641 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
5701 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
5761 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
5821 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
5881 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
5941 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
6001 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
6061 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
6121 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
6181 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
6241 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
6301 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
6361 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
6421 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
6481 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtc
//