LOCUS LT727443 5869 bp DNA circular SYN 06-FEB-2017
DEFINITION Vector pMxCAT, complete sequence.
ACCESSION LT727443
VERSION LT727443.1
KEYWORDS .
SOURCE Vector pMxCAT
ORGANISM Vector pMxCAT
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 5869)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..5869
/organism="Vector pMxCAT"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 3121."
/db_xref="taxon:1945354"
regulatory 13..458
/note="Mx"
/regulatory_class="promoter"
CDS 515..1174
/transl_table=11
/note="chloramphenicol resistance"
/protein_id="SJL88468.1"
/translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
A"
CDS <1315..1389
/codon_start=1
/transl_table=11
/note="part of SV40 small t-antigen"
/protein_id="SJL88469.1"
/translation="DLCEGTLLLWCDIIGQTTYRDLKL"
misc_feature 1390..1456
/note="intron of SV40 small t-antigen"
misc_feature 1457..2008
/note="3' UTR of SV40 small t-antigen"
regulatory 2009..2105
/note="polyA"
/regulatory_class="terminator"
regulatory complement(2009..2105)
/note="polyA"
/regulatory_class="terminator"
misc_feature complement(2106..2918)
/note="part of SV40 VP1"
regulatory complement(2974..3057)
/note="lac"
/regulatory_class="promoter"
rep_origin complement(3150..3550)
/note="pMB1 ori"
CDS complement(4092..4952)
/transl_table=11
/note="ampicillin resistance"
/protein_id="SJL88470.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
regulatory 5847..5863
/note="SP6"
/regulatory_class="promoter"
BASE COUNT 1555 a 1378 c 1373 g 1563 t
ORIGIN
1 aagcttgggc tgcagtagca gtgctcagac ctctaaacct gtagtcacaa gggacccaga
61 agggctggca gggctcctca gtgattctgg tatggtttgt ctctgaccct tcctagtgca
121 gctggaagaa tccagtgaaa agagaaagtt agtctaggtt gtgttcaact gagaggaggt
181 caggctgaag gctcagcaca gagctggaag ggtcctggga aagcaggcag ccatctgaac
241 tggtgtccat actgcatgtg tcttggtggg ctgagcctac aatatcgatt tcccaacctc
301 agtaccaagc caaggcccct cagggtgagt ttcgtttctg agctcctttc atatgtggtg
361 ggggtgtggg cacagcaagc tcaggctttt tcacggtttc aattctcctc tggaaggggc
421 ggagccagca ctatttaaag aggtctgagc cctggcagga ctctagagga tctcgaggag
481 cttggcgaga ttttcaggag ctaaggaagc taaaatggag aaaaaaatca ctggatatac
541 caccgttgat atatcccaat ggcatcgtaa agaacatttt gaggcatttc agtcagttgc
601 tcaatgtacc tataaccaga ccgttcagct ggatattacg gcctttttaa agaccgtaaa
661 gaaaaataag cacaagtttt atccggcctt tattcacatt cttgcccgcc tgatgaatgc
721 tcatccggaa ttccgtatgg caatgaaaga cggtgagctg gtgatatggg atagtgttca
781 cccttgttac accgttttcc atgagcaaac tgaaacgttt tcatcgctct ggagtgaata
841 ccacgacgat ttccggcagt ttctacacat atattcgcaa gatgtggcgt gttacggtga
901 aaacctggcc tatttcccta aagggtttat tgagaatatg tttttcgtct cagccaatcc
961 ctgggtgagt ttcaccagtt ttgatttaaa cgtggccaat atggacaact tcttcgcccc
1021 cgttttcacc atgggcaaat attatacgca aggcgacaag gtgctgatgc cgctggcgat
1081 tcaggttcat catgccgttt gtgatggctt ccatgtcggc agaatgctta atgaattaca
1141 acagtactgc gatgagtggc agggcggggc gtaatttttt taaggcagtt attggtgccc
1201 ttaaacgcct ggtgctacgc ctgaataagt gataataagc ggatgaatgg cagaaattcg
1261 ccggatcccc cctaggcttt tgcaaaaagc tcaagcttgg ctctagagtc gacggatctt
1321 tgtgaaggaa ccttacttct gtggtgtgac ataattggac aaactaccta cagagattta
1381 aagctctaag gtaaatataa aatttttaag tgtataatgt gttaaactac tgattctaat
1441 tgtttgtgta ttttagattc caacctatgg aactgatgaa tgggagcagt ggtggaatgc
1501 ctttaatgag gaaaacctgt tttgctcaga agaaatgcca tctagtgatg atgaggctac
1561 tgctgactct caacattcta ctcctccaaa aaagaagaga aaggtagaag accccaagga
1621 ctttccttca gaattgctaa gttttttgag tcatgctgtg tttagtaata gaactcttgc
1681 ttgctttgct atttacacca caaaggaaaa agctgcactg ctatacaaga aaattatgga
1741 aaaatattct gtaaccttta taagtaggca taacagttat aatcataaca tactgttttt
1801 tcttactcca cacaggcata gagtgtctgc tattaataac tatgctcaaa aattgtgtac
1861 ctttagcttt ttaatttgta aaggggttaa taaggaatat ttgatgtata gtgccttgac
1921 tagagatcat aatcagccat accacatttg tagaggtttt acttgcttta aaaaacctcc
1981 cacacctccc cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt aacttgttta
2041 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
2101 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct
2161 ggatcgatcc ccaggaagct cctctgtgtc ctcataaacc ctaacctcct ctacttgaga
2221 ggacattcca atcataggct gcccatccac cctctgtgtc ctcctgttaa ttaggtcact
2281 taacaaaaag gaaattgggt aggggttttt cacagaccgc tttctaaggg taattttaaa
2341 atatctggga agtcccttcc actgctgtgt tccagaagtg ttggtaaaca gcccacaaat
2401 gtcaacagca gaaacataca agctgtcagc tttgcacaag ggcccaacac cctgctcatc
2461 aagaagcact gtggttgctg tgttagtaat gtgcaaaaca ggaggcacat tttccccacc
2521 tgtgtaggtt ccaaaatatc tagtgttttc atttttactt ggatcaggaa cccagcactc
2581 cactggataa gcattatcct tatccaaaac agccttgtgg tcagtgttca tctgctgact
2641 gtcaactgta gcattttttg gggttacagt ttgagcagga tatttggtcc tgtagtttgc
2701 taacacaccc tgcagctcca aaggttcccc accaacagca aaaaaatgaa aatttgaccc
2761 ttgaatgggt tttccagcac cattttcatg agttttttgt gtccctgaat gcaagtttaa
2821 catagcagtt accccaataa cctcagtttt aacagtaaca gcttcccaca tcaaaatatt
2881 tccacaggtt aagtcctcat ttaaattagg caaaggaatt cgtaatcatg gtcatagctg
2941 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
3001 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca
3061 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc
3121 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg
3181 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
3241 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
3301 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
3361 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
3421 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
3481 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
3541 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
3601 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
3661 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
3721 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta
3781 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
3841 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
3901 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
3961 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
4021 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
4081 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
4141 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
4201 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
4261 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
4321 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
4381 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
4441 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
4501 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
4561 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
4621 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
4681 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
4741 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
4801 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
4861 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
4921 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
4981 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
5041 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt
5101 attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt
5161 ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt
5221 ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg
5281 tgtcggggct ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccatatc
5341 gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt
5401 tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg
5461 ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag
5521 cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc
5581 cggtgatgcc ggccacgatg cgtccggcgt agaggatctg gctagcgatg accctgctga
5641 ttggttcgct gaccatttcc ggggtgcgga acggcgttac cagaaactca gaaggttcgt
5701 ccaaccaaac cgactctgac ggcagtttac gagagagatg atagggtctg cttcagtaag
5761 ccagatgcta cacaattagg cttgtacata ttgtcgttag aacgcggcta caattaatac
5821 ataaccttat gtatcataca catacgattt aggtgacact atagaatac
//