LOCUS       LT727441                5130 bp    DNA     circular SYN 06-FEB-2017
DEFINITION  Mammalian expression vector pCDM8hFasR, complete sequence.
ACCESSION   LT727441
VERSION     LT727441.1
KEYWORDS    .
SOURCE      Mammalian expression vector pCDM8hFasR
  ORGANISM  Mammalian expression vector pCDM8hFasR
            other sequences; artificial sequences; vectors.
REFERENCE   1  (bases 1 to 5130)
  AUTHORS   De Schamphelaire W.
  JOURNAL   Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
            Technologiepark 927, 9052, BELGIUM.
REFERENCE   2
  AUTHORS   De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
            Roggeman Fonseca M., Vanhoucke M., Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished.
FEATURES             Location/Qualifiers
     source          1..5130
                     /organism="Mammalian expression vector pCDM8hFasR"
                     /mol_type="other DNA"
                     /note="BCCM/LMBP Plasmid collection (Ghent
                     University,Belgium) accession number LMBP 3118."
                     /db_xref="taxon:1944911"
     rep_origin      complement(36..111)
                     /note="SV40 ori"
     rep_origin      complement(262..374)
                     /note="polyoma ori"
     regulatory      1063..1159
                     /note="SV40 polyA late"
                     /regulatory_class="terminator"
     regulatory      complement(1063..1159)
                     /note="SV40 polyA early"
                     /regulatory_class="terminator"
     misc_feature    complement(1160..1558)
                     /note="3' UTR of SV40 small t-antigen"
     misc_feature    complement(1559..1625)
                     /note="intron of SV40 small t-antigen"
     CDS             complement(1626..>1700)
                     /codon_start=1
                     /transl_table=11
                     /note="part of SV40 small t-antigen"
                     /protein_id="SJL88463.1"
                     /translation="DLCEGTLLLWCDIIGQTTYRDLKL"
     CDS             complement(1703..2710)
                     /transl_table=11
                     /note="hFAS"
                     /protein_id="SJL88464.1"
                     /translation="MLGIWTLLPLVLTSVARLSSKSVNAQVTDINSKGLELRKTVTTV
                     ETQNLEGLHHDGQFCHKPCPPGERKARDCTVNGDEPDCVPCQEGKEYTDKAHFSSKCR
                     RCRLCDEGHGLEVEINCTRTQNTKCRCKPNFFCNSTVCEHCDPCTKCEHGIIKECTLT
                     SNTKCKEEGSRSNLGWLCLLLLPIPLIVWVKRKEVQKTCRKHRKENQGSHESPTLNPE
                     TVAINLSDVDLSKYITTIAGVMTLSQVKGFVRKNGVNEAKIDEIKNDNVQDTAEQKVQ
                     LLRNWHQLHGKKEAYDTLIKDLKKANLCTLAEKIQTIILKDITSDSENSNFRNEIQSL
                     V"
     misc_feature    complement(2711..2907)
                     /note="5' UTR of hFAS"
     regulatory      complement(2945..2961)
                     /note="T7 promoter"
                     /regulatory_class="promoter"
     regulatory      complement(3006..3589)
                     /note="hCMV-IE promoter and enhancer"
                     /regulatory_class="promoter"
     misc_feature    complement(3744..3946)
                     /note="supF"
     rep_origin      4070..4528
                     /note="M13 ori"
     rep_origin      complement(4547..4779)
                     /note="pMB1 ori"
BASE COUNT         1274 a         1221 c         1198 g         1434 t
ORIGIN      
        1 gaattagctt tttgcaaaag cctaggcctc caaaaaagcc tcctcactac ttctggaata
       61 gctcagaggc cgaggcggcc tcggcctctg cataaataaa aaaaattagt cagccatggg
      121 gcggagaatg ggcggaactg ggcggagtta ggggcgggat gggcggagta agacccgcac
      181 ccgttcccaa actgcttatt aaagggggta tggaggtgct ggaccttgtg acagggccag
      241 acagtgtgac agaaatagaa gcagtcctgt tttgacaagt tgcctctgga agcctctaca
      301 atgcctctct tctttttctc cagagtaagc gggcccaggg gtccccggcc tctgcttaat
      361 actaaaaaaa acagctgttg tcatagtaat gattgggtgg aaacattcta ggcctgggtg
      421 gagaggcttt tgcttcctct tgcaaaacca cacgccctct ggagggcgtt gcctagcaac
      481 taattaaaag aggatgtcgc acggccagct gcgtcagtta gtccacttcc tccttaactg
      541 acttgacatt ttctatttta agagtcggga ggaaaattac tgtgttggag gccctcgccc
      601 atcttctgaa gctgatcaag taagtgaatt ttcaaaatgg gagccgcact gactattcta
      661 gtagacctca tcgagggatt agctgaagtg tctaccctta cgggactctc ggcagaagct
      721 attttatctg gagaagccct cgctgccctt gatcgcgaaa ttacagctct cacttggtag
      781 ggtgtaatga gttcggagac agccctagca actatgggta tttcagagga ggtgtatggg
      841 tttgtcagta ctgtgcctgt gtttgtaagt cgaacagcgg gggctatatg gctgatgcag
      901 acagttcaag gtgcctctac tatttcccta ggaatacagc ggtacctaca caacgaagag
      961 gtccctactg taaatagaaa tatggcgttg ataccatggc gggatccaga catgataaga
     1021 tacattgatg agtttggaca aaccacaact agaatgcagt gataaaaatg ctttatttgt
     1081 gaaatttgtg atgctattgc tttatttgta accattataa gctgcaataa acaagttaac
     1141 aacaacaatt gcattcattt tatgtttcag gttcaggggg aggtgtggga ggttttttaa
     1201 agcaagtaaa acctctacaa atgtggtatg gctgattatg atctctagtc aaggcactat
     1261 acatcaaata tttttccata attttcttgt atagcagtgc agctttttcc tttgtggtgt
     1321 aaatagcaaa gcaagcaaga gttctattac taaacacagc atgactcaaa aaacttacca
     1381 attctgaagg aaagtccttg gggtcttcta cctttctctt cttttttgga ggagtagaat
     1441 gttgagagtc agcagtagcc tcatcatcac tagatggcat ttcttctgag caaaacaggt
     1501 tttcctcatt aaaggcattc caccactgct cccattcata agttccatag gttggaatct
     1561 aaaataccac aacaattaga atcagtagtt taacacatta tacacttaaa aattttatat
     1621 ttaccttaga gctttaaatc tctgtaggta gtttgtccaa ttatgtcaca ccacagaagt
     1681 aaggttcctt cacaaagatc ctctagacca agctttggat ttcatttctg aagtttgaat
     1741 tttctgagtc actagtaatg tccttgagga tgatagtctg aattttctct gcaagagtac
     1801 aaagattggc ttttttgaga tctttaatca atgtgtcata cgcttctttc tttccatgaa
     1861 gttgatgcca attacgaagc agttgaactt tctgttctgc tgtgtcttgg acattgtcat
     1921 tcttgatctc atctattttg gcttcattga caccattctt tcgaacaaag cctttaactt
     1981 gacttagtgt catgactcca gcaatagtgg tgatatattt actcaagtca acatcagata
     2041 aatttattgc cactgtttca ggatttaagg ttggagattc atgagaacct tggttttcct
     2101 ttctgtgctt tctgcatgtt ttctgtactt cctttctctt cacccaaaca attagtggaa
     2161 ttggcaaaag aagaagacaa agccacccca agttagatct ggatccttcc tctttgcact
     2221 tggtgttgct ggtgagtgtg cattccttga tgattccatg ttcacatttg gtgcaagggt
     2281 cacagtgttc acatacagta gagttacaaa aaaagtttgg tttacatctg cacttggtat
     2341 tctgggtccg ggtgcagttt atttccactt ctaagccatg tccttcatca cacaatctac
     2401 atcttctgca tttggaagaa aaatgggctt tgtctgtgta ctccttccct tcttggcagg
     2461 gcacgcagtc tggttcatcc ccattgactg tgcagtccct agctttcctt tcacctggag
     2521 gacagggctt atggcagaat tggccatcat gatgcaggcc ttccaagttc tgagtctcaa
     2581 ctgtagtaac agtcttcctc aattccaatc ccttggagtt gatgtcagtc acttgggcat
     2641 taacactttt ggacgataat ctagcaacag acgtaagaac cagaggtagg agggtccaga
     2701 tgcccagcat ggttgttgag caatcctccg aagtgaaaga gcttccccaa ctccgtactg
     2761 agcgggtcca ccaacccgcg ggagaagagg cagctccgcc tgggcagcca gggctggcct
     2821 cagggtgtgt tccgtgccag tgcccgcccc tgaggctcca gccaagtcac tcgtaaaccg
     2881 cttccctcac tccccagaag cgtcnnnctc gaggtcgagg gatctctaga agcttgggtc
     2941 tccctatagt gagtcgtatt aatttcgata agccagtaag cagtgggttc tctagttagc
     3001 cagagagctc tgcttatata gacctcccac cgtacacgcc taccgcccat ttgcgtcaat
     3061 ggggcggagt tgttacgaca ttttggaaag tcccgttgat tttggtgcca aaacaaactc
     3121 ccattgacgt caatggggtg gagacttgga aatccccgtg agtcaaaccg ctatccacgc
     3181 ccattgatgt actgccaaaa ccgcatcacc atggtaatag cgatgactaa tacgtagatg
     3241 tactgccaag taggaaagtc ccataaggtc atgtactggg cataatgcca ggcgggccat
     3301 ttaccgtcat tgacgtcaat agggggcgta cttggcatat gatacacttg atgtactgcc
     3361 aagtgggcag tttaccgtaa atagtccacc cattgacgtc aatggaaagt ccctattggc
     3421 gttactatgg gaacatacgt cattattgac gtcaatgggc gggggtcgtt gggcggtcag
     3481 ccaggcgggc catttaccgt aagttatgta acgcggaact ccatatatgg gctatgaact
     3541 aatgaccccg taattgatta ctattaataa ctagtcaata atcaatgtca acgcgtatat
     3601 ctggcccgta catcgcgaag cagcgcaaaa cgcctaaccc taagcagatt cttcatgcaa
     3661 ttgtcggtca agccttgcct tgttgtagct taaattttac tcgcgcacta ctcagcgacc
     3721 tccaacacac aagcagggag cagattcttt cggacttttg aaagtgatgg tggtggggga
     3781 aggattcgaa ccttcgaagt cgatgacggc agatttagag tctgctccct ttggccgctc
     3841 gggaacccca ccacgggtaa tgcttttact ggcctgctcc cttatcggga agcggggcgc
     3901 atcatatcaa atgacgcgcc gctgtaaagt gttacgttga gaaagaccgc ggtaatccag
     3961 ctggcgtacc gttcctgtct aaaatccctt taatcggcct cctgtttagc tcccgctctg
     4021 attccaacga ggaaagcacg ttatacggtc tcgtcaaagc aaccatagta cgcgccctgt
     4081 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
     4141 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
     4201 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg
     4261 cacctcgacc ccaaaaaact tgatttgggt gatggttcac gtagtgggcg gccatcgccc
     4321 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
     4381 ttccaaactg gaacaacact caaccctatc tcgggctatt cttttgattt ataagggatt
     4441 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
     4501 tttaacaaaa tattaacgtt tacaatttct agctagaagc tagcttgcgt tgctggcgtt
     4561 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     4621 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     4681 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     4741 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     4801 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     4861 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     4921 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     4981 taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac
     5041 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     5101 tttttttgtt tgcaagcagc agattacgcc
//