LOCUS LT727440 3302 bp DNA circular SYN 06-FEB-2017
DEFINITION Vector pMECAD, complete sequence.
ACCESSION LT727440
VERSION LT727440.1
KEYWORDS .
SOURCE Vector pMECAD
ORGANISM Vector pMECAD
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 3302)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..3302
/organism="Vector pMECAD"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 3111."
/db_xref="taxon:1945349"
CDS <415..>1030
/codon_start=2
/transl_table=11
/note="mouse Cdh1"
/protein_id="SJL88461.1"
/translation="PKNLVQIKSNRDKETKVFYSITGQGADKPPVGVFIIERETGWLK
VTQPLDREAIAKYILYSHAVSSNGEAVEDPMEIVITVTDQNDNRPEFTQEVFEGSVAE
GAVPGTSVMKVSATDADDDVNTYNAAIAYTIVSQDPELPHKNMFTVNRDTGVISVLTS
GLDRESYPTYTLVVQAADLQGEGLSTTAKAVITVKDINDNAPVFN"
regulatory complement(1124..1207)
/note="lac"
/regulatory_class="promoter"
rep_origin complement(1300..1700)
/note="pMB1 ori"
CDS complement(2242..3102)
/transl_table=11
/note="ampicillin resistance"
/protein_id="SJL88462.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
BASE COUNT 838 a 838 c 843 g 783 t
ORIGIN
1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt accccccaaa
421 gaacctggtt cagatcaaat ccaacaggga caaagaaaca aaggttttct acagcatcac
481 cggccaagga gctgacaaac cccccgttgg cgttttcatc attgagaggg agacaggctg
541 gctgaaagtg acacagcctc tggatagaga agccattgcc aagtacatcc tctattctca
601 tgccgtgtca tcaaatgggg aagcggtgga ggatcccatg gagatagtga tcacagtgac
661 agatcagaat gacaacaggc cagagtttac ccaggaggtg tttgagggat ccgttgcaga
721 aggcgctgtt ccaggaacct ccgtgatgaa ggtctcagcc accgatgcag acgatgacgt
781 caacacctac aacgctgcca tcgcctacac catcgtcagc caggatcctg agctgcctca
841 caaaaacatg ttcactgtca atagggacac cggggtcatc agtgtgctca cctctgggct
901 ggaccgagag agttacccta catacactct ggtggttcag gctgctgacc ttcaaggcga
961 aggcttgagc acaacagcca aggctgtgat cactgtcaag gatattaatg acaacgctcc
1021 tgtcttcaac ggggatcctc tagagtcgac ctgcaggcat gcaagcttgg cgtaatcatg
1081 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
1141 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
1201 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
1261 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
1321 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
1381 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
1441 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
1501 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
1561 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
1621 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
1681 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
1741 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
1801 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
1861 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
1921 aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
1981 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
2041 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
2101 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
2161 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
2221 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
2281 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
2341 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
2401 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
2461 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
2521 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
2581 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
2641 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
2701 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
2761 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
2821 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
2881 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
2941 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
3001 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
3061 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
3121 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
3181 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta
3241 agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg
3301 tc
//