LOCUS LT727432 3968 bp DNA circular SYN 06-FEB-2017
DEFINITION Vector pUC18kan1, complete sequence.
ACCESSION LT727432
VERSION LT727432.1
KEYWORDS .
SOURCE Vector pUC18kan1
ORGANISM Vector pUC18kan1
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 3968)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..3968
/organism="Vector pUC18kan1"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 3063."
/db_xref="taxon:1945417"
CDS 598..1413
/transl_table=11
/note="Tn903 neomycin resistance"
/protein_id="SJL88435.1"
/translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLY
GKPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIP
GKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGL
VDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVG
RVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
regulatory complement(1790..1873)
/note="lac promoter"
/regulatory_class="promoter"
rep_origin complement(1965..2367)
/note="pMB1 ori"
CDS complement(2908..3768)
/transl_table=11
/note="ampicillin resistance"
/protein_id="SJL88436.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
BASE COUNT 1003 a 959 c 993 g 1013 t
ORIGIN
1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
361 tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gcttgcatgc ctgcaggtcg
421 actctagagg atccccgggt accgagctcg aattccccgg atccgtcgac ctgcaggggg
481 gggggggaaa gccacgttgt gtctcaaaat ctctgatgtt acattgcaca agataaaaat
541 atatcatcat gaacaataaa actgtctgct tacataaaca gtaatacaag gggtgttatg
601 agccatattc aacgggaaac gtcttgctcg aggccgcgat taaattccaa catggatgct
661 gatttatatg ggtataaatg ggctcgcgat aatgtcgggc aatcaggtgc gacaatctat
721 cgattgtatg ggaagcccga tgcgccagag ttgtttctga aacatggcaa aggtagcgtt
781 gccaatgatg ttacagatga gatggtcaga ctaaactggc tgacggaatt tatgcctctt
841 ccgaccatca agcattttat ccgtactcct gatgatgcat ggttactcac cactgcgatc
901 cccgggaaaa cagcattcca ggtattagaa gaatatcctg attcaggtga aaatattgtt
961 gatgcgctgg cagtgttcct gcgccggttg cattcgattc ctgtttgtaa ttgtcctttt
1021 aacagcgatc gcgtatttcg tctcgctcag gcgcaatcac gaatgaataa cggtttggtt
1081 gatgcgagtg attttgatga cgagcgtaat ggctggcctg ttgaacaagt ctggaaagaa
1141 atgcataagc ttttgccatt ctcaccggat tcagtcgtca ctcatggtga tttctcactt
1201 gataacctta tttttgacga ggggaaatta ataggttgta ttgatgttgg acgagtcgga
1261 atcgcagacc gataccagga tcttgccatc ctatggaact gcctcggtga gttttctcct
1321 tcattacaga aacggctttt tcaaaaatat ggtattgata atcctgatat gaataaattg
1381 cagtttcatt tgatgctcga tgagtttttc taatcagaat tggttaattg gttgtaacac
1441 tggcagagca ttacgctgac ttgacgggac ggcggctttg ttgaataaat cgaacttttg
1501 ctgagttgaa ggatcagatc acgcatcttc ccgacaacgc agaccgttcc gtggcaaagc
1561 aaaagttcaa aatcaccaac tggtccacct acaacaaagc tctcatcaac cgtggctccc
1621 tcactttctg gctggatgat ggggcgattc aggcctggta tgagtcagca acaccttctt
1681 cacgaggcag acctcagcgc cccccccccc ctgcaggtcg acggatccgg ggaattcgta
1741 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat
1801 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt
1861 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta
1921 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc
1981 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
2041 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
2101 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
2161 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
2221 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
2281 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
2341 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
2401 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
2461 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
2521 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
2581 cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
2641 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
2701 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
2761 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
2821 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
2881 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
2941 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
3001 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
3061 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
3121 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
3181 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
3241 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
3301 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
3361 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
3421 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
3481 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
3541 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
3601 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
3661 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
3721 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
3781 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
3841 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga
3901 cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc
3961 ctttcgtc
//