LOCUS LT727428 8486 bp DNA circular SYN 06-FEB-2017
DEFINITION Vector pLPPsOmpArPDI, complete sequence.
ACCESSION LT727428
VERSION LT727428.1
KEYWORDS .
SOURCE Vector pLPPsOmpArPDI
ORGANISM Vector pLPPsOmpArPDI
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 8486)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..8486
/organism="Vector pLPPsOmpArPDI"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 3056."
/db_xref="taxon:1945326"
regulatory 24..208
/note="lpp promoter"
/regulatory_class="promoter"
regulatory 235..291
/note="lacUV5 promoter"
/regulatory_class="promoter"
misc_feature 292..329
/note="5' UTR of lacZ, incl. RBS"
CDS 351..>413
/transl_table=11
/note="ompA signal sequence"
/protein_id="SJL88422.1"
/translation="MKKTAIAIAVALAGFATVAQA"
CDS <414..>419
/codon_start=1
/transl_table=11
/note="part of rPDI signal sequence"
/protein_id="SJL88423.1"
/translation="GA"
CDS <420..1889
/codon_start=1
/transl_table=11
/note="rPDI mature sequence"
/protein_id="SJL88424.1"
/translation="DALEEEDNVLVLKKSNFAEPAAHNYLLVEFYAPWCGHCKALAPE
YAKAAAKLKAEGSEIRLAKVDATEESDLAQQYGVRGYPTIKFFKNGDTASPKEYTAGR
EADDIVNWLKKRTGPAATTLSDTAAAESLVDSSEVTVIGFFKDAGSDSAKQFLLAAEA
VDDIPFGITSNSDVFSKYQLDKDGVVLFKKFDEGRNNFEGEITKEKLLDFIKHNQLPL
VIEFTEQTAPKIFGGEIKTHILLFLPKSVSDYDGKLSNFKKAAEGFKGKILFIFIDSD
HTDNQRILEFFGLKKEECPAVRLITLEEEMTKYKPESDELTAEKITQFCHHFLEGKIK
PHLMSQELPEDWDKQPVKVLVGKNFEEVAFDEKKNVFVEFYAPWCGHCKQLAPIWDKL
GETYKDHENIVIAKMDSTANEVEAVKVHSFPTLKFFPASADRTVIDYNGERTLDGFKK
FLESGGQDGAGDNDDLDLEEALEPDMEEDDDQKAVKDEL"
regulatory 2016..2082
/note="lpp terminator"
/regulatory_class="terminator"
regulatory 2784..2861
/note="lacIq promoter"
/regulatory_class="promoter"
CDS 2862..3944
/transl_table=11
/note="lacI"
/protein_id="SJL88425.1"
/translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAE
LNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERS
GVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSII
FSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREG
DWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDT
EDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNT
QTASPRALADSLMQLARQVSRLESGQ"
rep_origin complement(6479..6881)
/note="pMB1 ori"
CDS complement(7422..8282)
/transl_table=11
/note="ampicillin"
/protein_id="SJL88426.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
BASE COUNT 2094 a 2218 c 2194 g 1980 t
ORIGIN
1 ataaccagaa gcaataaaaa atcaaatcgg atttcactat ataatctcac tttatctaag
61 atgaatccga tggaagcatc ctgttttctc tcaatttttt tatctaaaac ccagcgttcg
121 atgcttcttt gagcgaacga tcaaaaataa gtgccttccc atcaaaaaaa tattctcaac
181 ataaaaaact ttgtgtaata cttgtaacgc tacatggaga ttaactcaat ctagcactca
241 ttaggcaccc caggctttac actttatgct tccggctcgt ataatgtgtg gaattgtgag
301 cggataacaa tttcacacag gaaacagctc tagataacga ggcgcaaaaa atgaaaaaga
361 cagctatcgc gattgcagtg gcactggctg gtttcgctac cgtagcgcag gccggcgccg
421 acgctctgga ggaggaggac aacgtcctgg tgctgaagaa gagcaacttc gcagagccgg
481 cggcgcacaa ctacctgctg gtggagttct atgccccatg gtgtggccac tgcaaagcac
541 tggccccaga gtatgccaaa gctgctgcaa aactgaaggc agaaggctct gagatccgac
601 tagcaaaggt ggacgccaca gaagagtctg acctggccca gcagtatggt gtccgtggct
661 accccacaat caagttcttc aagaatggag acacagcctc cccaaaggaa tatacagctg
721 gcagggaagc tgacgacatt gtgaactggc tgaagaaacg cacaggccca gcagccacaa
781 ccctgtctga cactgcagct gcagagtcct tggtggactc aagcgaagtg acggtcatcg
841 gcttcttcaa ggacgcaggg tcagactccg ccaagcagtt cttgctggca gcagaggctg
901 ttgatgacat accttttgga atcacttcca atagcgatgt gttttccaag taccagctgg
961 acaaggatgg ggtggtcctc tttaagaagt ttgatgaagg ccgcaacaat tttgaaggtg
1021 agatcaccaa ggagaagcta ttagacttca tcaagcacaa ccagctgcct ttggtcatcg
1081 agttcactga acagacagct ccaaagattt tcggaggtga aatcaagaca catattctgc
1141 tgttcctgcc caagagtgtg tctgactacg atggcaaatt gagcaacttt aagaaagcgg
1201 ccgagggctt taagggcaag atcctgttca tcttcatcga tagtgaccac actgacaacc
1261 agcgcatact tgagttcttt ggcctgaaga aggaggaatg tccagctgtg cggcttatta
1321 ccctggagga agagatgacc aagtacaaac cggagtcaga cgagctgaca gctgagaaga
1381 tcacacaatt ttgccaccac ttcctggagg gcaagatcaa gccccacctg atgagccagg
1441 aactgcctga agactgggac aagcagccag tgaaagtgct agttgggaaa aactttgagg
1501 aggttgcttt tgatgagaaa aagaacgtgt ttgttgaatt ctatgctccc tggtgtggtc
1561 actgcaagca gctagccccg atttgggata aactgggaga gacatacaaa gaccatgaga
1621 atatcgtcat cgctaagatg gactcaacag ccaatgaggt ggaagctgtg aaggtgcaca
1681 gctttcccac actcaagttc ttcccagcaa gtgcagacag aacggtcatt gattacaacg
1741 gtgagcggac actagatggt tttaagaaat tcttggagag cggtggccag gatggagcgg
1801 gggacaatga cgacctcgac ctagaagaag ctttagagcc agatatggaa gaagacgacg
1861 atcagaaagc cgtgaaggat gaactgtagt gcagaagcca gatccggctg agcaacgacg
1921 tgaacgcaat gcgttccgac gttcaggctg ctaaagatga cgcagctcgt gctaaccagc
1981 gtctggacaa catggctact aaataccgca agtaatagta cctgtgaagt gaaaaatggc
2041 gcacattgtg cgccattttt tttgtctgcc gtttaccgct actgcgtcac gcgtaacata
2101 ttcccttgct ctggttcacc attctgcgct gactctactg aaggcgcatt gctggctgcg
2161 ggagttgctc cactgctcac cgaaaccgga taccctgccc gacgatacaa cgctttatcg
2221 actaacttct gatctacagc cttattgtct ttaaattgcg taaagcctgc tggcagtgtg
2281 tatggcattg tctgaacgtt ctgctgttct tctgccgata gtggtcgatg tacttcaaca
2341 taacgcatcc cgttaggctc cacggaatat ttcaccggtt cgttgatcac tttcaccggc
2401 gttcccgtcc gcacgctgga gaacaaggct ttaatatccg gtgcattcat gcgaatacac
2461 cctgaactga cgcgcaaacc gacgctgtcc ggcgcactgg taccatgaat gaggtattcg
2521 ccattaccat gcgcgaggcg cagtgcgtaa cgtcctagcg ggttatttgg tccggcagga
2581 acgactggcg gtaatttaat gccacgctcc agcgaacgct gacgaatgcc tgccgtaggc
2641 gtccaggttg ggttagggat tttctgccca acacgcgttt ccatcaccgg cgtttccagc
2701 ccctgcaatc caatacctat tggataaacc tgcacaatat tttctcccgg cggataataa
2761 taaaggcgca gctctgcaag gttgacacca tcgaatggtg caaaaccttt cgcggtatgg
2821 catgatagcg cccggaagag agtcaattca gggtggtgaa tgtgaaacca gtaacgttat
2881 acgatgtcgc agagtatgcc ggtgtctctt atcagaccgt ttcccgcgtg gtgaaccagg
2941 ccagccacgt ttctgcgaaa acgcgggaaa aagtggaagc ggcgatggcg gagctgaatt
3001 acattcccaa ccgcgtggca caacaactgg cgggcaaaca gtcgttgctg attggcgttg
3061 ccacctccag tctggccctg cacgcgccgt cgcaaattgt cgcggcgatt aaatctcgcg
3121 ccgatcaact gggtgccagc gtggtggtgt cgatggtaga acgaagcggc gtcgaagcct
3181 gtaaagcggc ggtgcacaat cttctcgcgc aacgcgtcag tgggctgatc attaactatc
3241 cgctggatga ccaggatgcc attgctgtgg aagctgcctg cactaatgtt ccggcgttat
3301 ttcttgatgt ctctgaccag acacccatca acagtattat tttctcccat gaagacggta
3361 cgcgactggg cgtggagcat ctggtcgcat tgggtcacca gcaaatcgcg ctgttagcgg
3421 gcccattaag ttctgtctcg gcgcgtctgc gtctggctgg ctggcataaa tatctcactc
3481 gcaatcaaat tcagccgata gcggaacggg aaggcgactg gagtgccatg tccggttttc
3541 aacaaaccat gcaaatgctg aatgagggca tcgttcccac tgcgatgctg gttgccaacg
3601 atcagatggc gctgggcgca atgcgcgcca ttaccgagtc cgggctgcgc gttggtgcgg
3661 atatctcggt agtgggatac gacgataccg aagacagctc atgttatatc ccgccgttaa
3721 ccaccatcaa acaggatttt cgcctgctgg ggcaaaccag cgtggaccgc ttgctgcaac
3781 tctctcaggg ccaggcggtg aagggcaatc agctgttgcc cgtctcactg gtgaaaagaa
3841 aaaccaccct ggcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa
3901 tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat
3961 gtgagttagc tcactcatta ggcaccccag gctttacact ttatgctaac gataatcccc
4021 tgacgcggtg catcaggtaa taacagttgt gaaggaatag ttatcgtcgt accaggtttt
4081 ggcaccgggg cgatagtgtt attggcttca aggatcaaca ttgccgcagt atcaaaacgt
4141 cgggcaatag cctgaaggtt tttatcccct tcttgcaccg tatacgtttg attttgccca
4201 accagtcggc ttccggttgg tggtagcgga taatcaaccg cccaggcagc ctggatggcg
4261 ctaaaagcgc cgataagcgt gagtgtaagc aaagacgcgc gtttcattgt aaacctcctg
4321 tatttgccgg agactcacgc tgaaacgtcg gatggcgctt atgttcacct gaaaccaaaa
4381 cactcctgtg caggtcagtg taaacattga ccatccggca atgtgagcca accggatgaa
4441 agctgtcctt ttagtttagc taagtgcagc ggctttggcg cgaattgcgc gaatcatcgc
4501 ttccagacct tgtgaacgag atggggtgag atgttgggtg agcgccattt tttcaaacca
4561 cggacgcaca tcgaaattga caatatcctg cggcgtcatc tgatcgtaga gaataaagac
4621 gaccgcaata agccctttca caatcgccgc atcgctgtcg ccctgtaatt caataattcc
4681 ctgggcattc tggcgcatga caatccacac ctgactctga cagccctgaa tgctattttg
4741 tggacttctg tcttcgtcgc gtaattctgg cagacgctgg gggaccgatg cccttgagag
4801 ccttcaaccc agtcagctcc ttccggtggg cgcggggcat gactatcgtc gccgcactta
4861 tgactgtctt ctttatcatg caactcgtag gacaggtgcc ggcagcgctc tgggtcattt
4921 tcggcgagga ccgctttcgc tggagcgcga cgatgatcgg cctgtcgctt gcggtattcg
4981 gaatcttgca cgccctcgct caagccttcg tcactggtcc cgccaccaaa cgtttcggcg
5041 agaagcaggc cattatcgcc ggcatggcgg ccgacgcgct gggctacgtc ttgctggcgt
5101 tcgcgacgcg aggctggatg gccttcccca ttatgattct tctcgcttcc ggcggcatcg
5161 ggatgcccgc gttgcaggcc atgctgtcca ggcaggtaga tgacgaccat cagggacagc
5221 ttcaaggatc gctcgcggct cttaccagcc taacttcgat cactggaccg ctgatcgtca
5281 cggcgattta tgccgcctcg gcgagcacat ggaacgggtt ggcatggatt gtaggcgccg
5341 ccctatacct tgtctgcctc cccgcgttgc gtcgcggtgc atggagccgg gccacctcga
5401 cctgaatgga agccggcggc acctcgctaa cggattcacc actccaagaa ttggagccaa
5461 tcaattcttg cggagaactg tgaatgcgca aaccaaccct tggcagaaca tatccatcgc
5521 gtccgccatc tccagcagcc gcacgcggcg catctcgggc agcgttgggt cctggccacg
5581 ggtgcgcatg atcgtgctcc tgtcgttgag gacccggcta ggctggcggg gttgccttac
5641 tggttagcag aatgaatcac cgatacgcga gcgaacgtga agcgactgct gctgcaaaac
5701 gtctgcgacc tgagcaacaa catgaatggt cttcggtttc cgtgtttcgt aaagtctgga
5761 aacgcggaag tcagcgccct gcaccattat gttccggatc tgcatcgcag gatgctgctg
5821 gctaccctgt ggaacaccta catctgtatt aacgaagcgc tggcattgac cctgagtgat
5881 ttttctctgg tcccgccgca tccataccgc cagttgttta ccctcacaac gttccagtaa
5941 ccgggcatgt tcatcatcag taacccgtat cgtgagcatc ctctctcgtt tcatcggtat
6001 cattaccccc atgaacagaa atccccctta cacggaggca tcagtgacca aacaggaaaa
6061 aaccgccctt aacatggccc gctttatcag aagccagaca ttaacgcttc tggagaaact
6121 caacgagctg gacgcggatg aacaggcaga catctgtgaa tcgcttcacg accacgctga
6181 tgagctttac cgcagctgcc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat
6241 gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg
6301 tcagggcgcg tcagcgggtg ttggcgggtg tcggggcgca gccatgaccc agtcacgtag
6361 cgatagcgga gtgtatactg gcttaactat gcggcatcag agcagattgt actgagagtg
6421 caccatatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggcgc
6481 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
6541 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
6601 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
6661 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
6721 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
6781 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
6841 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
6901 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
6961 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
7021 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
7081 cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt
7141 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
7201 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
7261 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
7321 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
7381 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
7441 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
7501 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
7561 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
7621 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
7681 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctgca
7741 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
7801 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
7861 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
7921 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
7981 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaaca
8041 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
8101 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
8161 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
8221 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
8281 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
8341 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
8401 aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg
8461 cgtatcacga ggccctttcg tcttca
//