LOCUS LT727427 11096 bp DNA circular SYN 06-FEB-2017
DEFINITION Mammalian expression vector pSV71rPDI2, complete sequence.
ACCESSION LT727427
VERSION LT727427.1
KEYWORDS .
SOURCE Mammalian expression vector pSV71rPDI2
ORGANISM Mammalian expression vector pSV71rPDI2
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 11096)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..11096
/organism="Mammalian expression vector pSV71rPDI2"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 3054."
/db_xref="taxon:1945134"
regulatory 101..382
/note="SV40 early promoter"
/regulatory_class="promoter"
rep_origin 339..414
/note="SV40 ori"
misc_feature 453..698
/note="SV40 large T-antigen"
CDS 453..977
/transl_table=11
/note="SV40 small t-antigen"
/protein_id="SJL88418.1"
/translation="MDKVLNREESLQLMDLLGLERSAWGNIPLMRKAYLKKCKEFHPD
KGGDEEKMKKMNTLYKKMEDGVKYAHQPDFGGFWDATEVFASSLNPGVDAMYCKQWPE
CAKKMSANCICLLCLLRMKHENRKLYRKDPLVWVDCYCFDCFRMWFGLDLCEGTLLLW
CDIIGQTTYRDLKL"
misc_feature 699..1044
/note="intron"
misc_feature 1045..2925
/note="SV40 large T-antigen"
regulatory complement(2926..3022)
/note="polyA"
/regulatory_class="terminator"
regulatory 2926..3022
/note="polyA"
/regulatory_class="terminator"
CDS complement(4008..>5477)
/codon_start=1
/transl_table=11
/note="rPDI"
/protein_id="SJL88419.1"
/translation="DALEEEDNVLVLKKSNFAEPAAHNYLLVEFYAPWCGHCKALAPE
YAKAAAKLKAEGSEIRLAKVDATEESDLAQQYGVRGYPTIKFFKNGDTASPKEYTAGR
EADDIVNWLKKRTGPAATTLSDTAAAESLVDSSEVTVIGFFKDAGSDSAKQFLLAAEA
VDDIPFGITSNSDVFSKYQLDKDGVVLFKKFDEGRNNFEGEITKEKLLDFIKHNQLPL
VIEFTEQTAPKIFGGEIKTHILLFLPKSVSDYDGKLSNFKKAAEGFKGKILFIFIDSD
HTDNQRILEFFGLKKEECPAVRLITLEEEMTKYKPESDELTAEKITQFCHHFLEGKIK
PHLMSQELPEDWDKQPVKVLVGKNFEEVAFDEKKNVFVEFYAPWCGHCKQLAPIWDKL
GETYKDHENIVIAKMDSTANEVEAVKVHSFPTLKFFPASADRTVIDYNGERTLDGFKK
FLESGGQDGAGDNDDLDLEEALEPDMEEDDDQKAVKDEL"
misc_feature complement(5478..5534)
/note="srPDI"
misc_feature complement(5605..6542)
/note="intron"
misc_feature complement(6543..6745)
/note="5UTR"
regulatory complement(6746..7041)
/note="SV40 late promoter"
/regulatory_class="promoter"
regulatory 6797..7078
/note="SV40 early promoter"
/regulatory_class="promoter"
rep_origin 7035..7110
/note="SV40 ori"
misc_feature 7149..7394
/note="SV40 large T-antigen"
CDS 7149..7673
/transl_table=11
/note="SV40 small t-antigen"
/protein_id="SJL88420.1"
/translation="MDKVLNREESLQLMDLLGLERSAWGNIPLMRKAYLKKCKEFHPD
KGGDEEKMKKMNTLYKKMEDGVKYAHQPDFGGFWDATEVFASSLNPGVDAMYCKQWPE
CAKKMSANCICLLCLLRMKHENRKLYRKDPLVWVDCYCFDCFRMWFGLDLCEGTLLLW
CDIIGQTTYRDLKL"
misc_feature 7395..7740
/note="intron"
misc_feature 7741..8803
/note="part of SV40 large t-antigen"
rep_origin complement(9080..9490)
/note="pMB1 ori"
CDS complement(10201..10860)
/transl_table=11
/note="chloramphenicol resistance"
/protein_id="SJL88421.1"
/translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
A"
BASE COUNT 3148 a 2393 c 2559 g 2987 t
ORIGIN
1 ttctcatgtt tgacagctta tcatcggcgc agcaccatgg cctgaaataa cctctgaaag
61 aggaacttgg ttaggtacct tctgaggcgg aaagaaccag ctgtggaatg tgtgtcagtt
121 agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
181 ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
241 catgcatctc aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct
301 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
361 agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg
421 aggcctaggc ttttgcaaaa agctttgcaa agatggataa agttttaaac agagaggaat
481 ctttgcagct aatggacctt ctaggtcttg aaaggagtgc ctgggggaat attcctctga
541 tgagaaaggc atatttaaaa aaatgcaagg agtttcatcc tgataaagga ggagatgaag
601 aaaaaatgaa gaaaatgaat actctgtaca agaaaatgga agatggagta aaatatgctc
661 atcaacctga ctttggaggc ttctgggatg caactgaggt atttgcttct tccttaaatc
721 ctggtgttga tgcaatgtac tgcaaacaat ggcctgagtg tgcaaagaaa atgtctgcta
781 actgcatatg cttgctgtgc ttactgagga tgaagcatga aaatagaaaa ttatacagga
841 aagatccact tgtgtgggtt gattgctact gcttcgattg ctttagaatg tggtttggac
901 ttgatctttg tgaaggaacc ttacttctgt ggtgtgacat aattggacaa actacctaca
961 gagatttaaa gctctaaggt aaatataaaa tttttaagtg tataatgtgt taaactactg
1021 attctaattg tttgtgtatt ttagattcca acctatggaa ctgatgaatg ggagcagtgg
1081 tggaatgcct ttaatgagga aaacctgttt tgctcagaag aaatgccatc tagtgatgat
1141 gaggctactg ctgactctca acattctact cctccaaaaa agaagagaaa ggtagaagac
1201 cccaaggact ttccttcaga attgctaagt tttttgagtc atgctgtgtt tagtaataga
1261 actcttgctt gctttgctat ttacaccaca aaggaaaaag ctgcactgct atacaagaaa
1321 attatggaaa aatattctgt aacctttata agtaggcata acagttataa tcataacata
1381 ctgttttttc ttactccaca caggcataga gtgtctgcta ttaataacta tgctcaaaaa
1441 ttgtgtacct ttagcttttt aatttgtaaa ggggttaata aggaatattt gatgtatagt
1501 gccttgacta gagatccatt ttctgttatt gaggaaagtt tgccaggtgg gttaaaggag
1561 catgatttta atccagaaga agcagaggaa actaaacaag tgtcctggaa gcttgtaaca
1621 gagtatgcaa tggaaacaaa atgtgatgat gtgttgttat tgcttgggat gtacttggaa
1681 tttcagtaca gttttgaaat gtgtttaaaa tgtattaaaa aagaacagcc cagccactat
1741 aagtaccatg aaaagcatta tgcaaatgct gctatatttg ctgacagcaa aaaccaaaaa
1801 accatatgcc aacaggctgt tgatactgtt ttagctaaaa agcgggttga tagcctacaa
1861 ttaactagag aacaaatgtt aacaaacaga tttaatgatc ttttggatag gatggatata
1921 atgtttggtt ctacaggctc tgctgacata gaagaatgga tggctggagt tgcttggcta
1981 cactgtttgt tgcccaaaat ggattcagtg gtgtatgact ttttaaaatg catggtgtac
2041 aacattccta aaaaaagata ctggctgttt aaaggaccaa ttgatagtgg taaaactaca
2101 ttagcagctg ctttgcttga attatgtggg gggaaagctt taaatgttaa tttgcccttg
2161 gacaggctga actttgagct aggagtagct attgaccagt ttttagtagt ttttgaggat
2221 gtaaagggca ctggagggga gtccagagat ttgccttcag gtcagggaat taataacctg
2281 gacaatttaa gggattattt ggatggcagt gttaaggtaa acttagaaaa gaaacaccta
2341 aataaaagaa ctcaaatatt tccccctgga atagtcacca tgaatgagta cagtgtgcct
2401 aaaacactgc aggccagatt tgtaaaacaa atagatttta ggcccaaaga ttatttaaag
2461 cattgcctgg aacgcagtga gtttttgtta gaaaagagaa taattcaaag tggcattgct
2521 ttgcttctta tgttaatttg gtacagacct gtggctgagt ttgctcaaag tattcagagc
2581 agaattgtgg agtggaaaga gagattggac aaagagttta gtttgtcagt gtatcaaaaa
2641 atgaagttta atgtggctat gggaattgga gttttagatt ggctaagaaa cagtgatgat
2701 gatgatgaag acagccagga aaatgctgat aaaaatgaag atggtgggga gaagaacatg
2761 gaagactcag ggcatgaaac aggcattgat tcacagtccc aaggctcatt tcaggcccct
2821 cagtcctcac agtctgttca tgatcataat cagccatacc acatttgtag aggttttact
2881 tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt
2941 tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
3001 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
3061 tgtatcttat catgtctgga tccggctcta gagggggnnn ggatccnnng aattcnnnat
3121 ggtagtaaaa tttcaacttt atttagccaa tgtgttcaat tccaacatta tggtttagaa
3181 atagaccttg agaatgggca tttcattcag ccccagtgtc cttagctgac tcccagtctg
3241 ggaatcaagg gtcccggttc tgcctcacac aggccacatg ccatgctgga agaggatcca
3301 gcagccagag taatgtgccc acagccggcc ggctggtcgg tagttctggc ttaagaagag
3361 tgagagctgt ccaggcctgg cctgagcctg gcagcaagcc accactgtct cttttccaca
3421 cgtggggacc caccctctga caggatgcag aaatccccag gttaaatgca aactacaaaa
3481 gccatgctgt catggcaaca ctagtacaag gtggagagag agagagcata cgacccagaa
3541 ccatcgcctc ctgcgcaggg caggaagggg tctcagggag gtctgcgtgg tggaacaagt
3601 acctcgacat ggagacatgt caggaagatg aaacatctca cagacgaaat ctgaagtgat
3661 agcataaacc tctctaacag gaaagacatt accagtgtga aatccccaaa tatggtagca
3721 agcacttggg acaaaatcca cgtacaaaaa agtacaccac caattaaaac aaaacgtagc
3781 ccttcaagat gggctggcct ggacagagat ccctttccaa aattgaaaag caaaaaaaga
3841 agagacaggt ttaagtgtac gtgtgtcaga tgagtgtggc ttcagaaaga ttccctgggt
3901 tctgatggcg ctccctcctg gggtgaggct caggctccgg agatgtgggc tgctgggaca
3961 tggcccaccg aggttttggg ttcaggcgcc cagatctggc ttctgcacta cagttcatcc
4021 ttcacggctt tctgatcgtc gtcttcttcc atatctggct ctaaagcttc ttctaggtcg
4081 aggtcgtcat tgtcccccgc tccatcctgg ccaccgctct ccaagaattt cttaaaacca
4141 tctagtgtcc gctcaccgtt gtaatcaatg accgttctgt ctgcacttgc tgggaagaac
4201 ttgagtgtgg gaaagctgtg caccttcaca gcttccacct cattggctgt tgagtccatc
4261 ttagcgatga cgatattctc atggtctttg tatgtctctc ccagtttatc ccaaatcggg
4321 gctagctgct tgcagtgacc acaccaggga gcatagaatt caacaaacac gttctttttc
4381 tcatcaaaag caacctcctc aaagtttttc ccaactagca ctttcactgg ctgcttgtcc
4441 cagtcttcag gcagttcctg gctcatcagg tggggcttga tcttgccctc caggaagtgg
4501 tggcaaaatt gtgtgatctt ctcagctgtc agctcgtctg actccggttt gtacttggtc
4561 atctcttcct ccagggtaat aagccgcaca gctggacatt cctccttctt caggccaaag
4621 aactcaagta tgcgctggtt gtcagtgtgg tcactatcga tgaagatgaa caggatcttg
4681 cccttaaagc cctcggccgc tttcttaaag ttgctcaatt tgccatcgta gtcagacaca
4741 ctcttgggca ggaacagcag aatatgtgtc ttgatttcac ctccgaaaat ctttggagct
4801 gtctgttcag tgaactcgat gaccaaaggc agctggttgt gcttgatgaa gtctaatagc
4861 ttctccttgg tgatctcacc ttcaaaattg ttgcggcctt catcaaactt cttaaagagg
4921 accaccccat ccttgtccag ctggtacttg gaaaacacat cgctattgga agtgattcca
4981 aaaggtatgt catcaacagc ctctgctgcc agcaagaact gcttggcgga gtctgaccct
5041 gcgtccttga agaagccgat gaccgtcact tcgcttgagt ccaccaagga ctctgcagct
5101 gcagtgtcag acagggttgt ggctgctggg cctgtgcgtt tcttcagcca gttcacaatg
5161 tcgtcagctt ccctgccagc tgtatattcc tttggggagg ctgtgtctcc attcttgaag
5221 aacttgattg tggggtagcc acggacacca tactgctggg ccaggtcaga ctcttctgtg
5281 gcgtccacct ttgctagtcg gatctcagag ccttctgcct tcagttttgc agcagctttg
5341 gcatactctg gggccagtgc tttgcagtgg ccacaccatg gggcatagaa ctccaccagc
5401 aggtagttgt gcgccgccgg ctctgcgaag ttgctcttct tcagcaccag gacgttgtcc
5461 tcctcctcca gagcgtcggc gcccacccta gccgcccagg ccagggccag gcacagcaaa
5521 gcacggctca gcatgtcggc ctctagaggc tgcagccgac cccggatccg gagcttttag
5581 agcagaagta acacttccgt acaggcctag aagtaaaggc aacatccact gaggagcagt
5641 tctttgattt gcaccaccag gagcctcaaa tttttcaata aattcacctg actgcacatt
5701 aggactttgg ctttgagctt cccacctctc agttacttgc tgaatactgt ctgcttcatc
5761 aatattatca taggtgtgcc caaatgatat ttgcaaccct tccctgttgg ctacttgtct
5821 caccattgta ggcctaatgg gagacaaagt agagtagtaa tcttgtaaag agttatacca
5881 attaacagga gcattaatta ctgtccaagt agtttcctct aaaaaccttg ccaaactgtc
5941 ccttaaatat ctttgggttc ttctttcaag ctcctgtgag gtgagcctag gaatgtcatt
6001 ttgtattaca cgccaaaaag cttgagaaat ggcattaaaa agtgttggac cccaatgtct
6061 ggggtcaaga tactgaacac tgtgaacaaa ggtttgtact ccaggaaata aaatatcata
6121 gtaatcatct ggcctataca aatctacagc cattcctggt tgttgatata aaccaacagt
6181 agaaactttg tgatcccagt cactaaaaaa tctatacccc acttgagcaa cagcgctcac
6241 accagtcaca gtttgcagta aagctgcaaa tccagctata gcagcaggag ccccagatat
6301 cacagcatag gcctgtggag tgaggcctat agcagcaatt gcctcagagg ttgttaggcc
6361 ttcaacagta gcaacagatg caagttgcac ttcaattgca gcagcggcct ctccagcagc
6421 aatttcagct actgaaaatc cagtagcagc agcagcttca gacacagtag caattaggtc
6481 ccccaacagt gttaaagcag cacccatgga cctgaaataa aagacaaaaa gactaaactt
6541 accagttaac tttctggttt ttcagttaac ctttctggtt ttttgcgttt cccgtcaaca
6601 gtatcttccc cttcacaaaa ttgcagcaaa agctctaaaa caaacacaaa aaggcgttga
6661 gctgtttttt tactttcagt ccatgaccta cgaaccttaa cggaggcctg gcgtgacagc
6721 cggcgcagca ccatggcctg aaataacctc tgaaagagga acttggttag gtaccttctg
6781 aggcggaaag aaccagctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc
6841 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa
6901 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
6961 catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc
7021 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc
7081 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct
7141 ttgcaaagat ggataaagtt ttaaacagag aggaatcttt gcagctaatg gaccttctag
7201 gtcttgaaag gagtgcctgg gggaatattc ctctgatgag aaaggcatat ttaaaaaaat
7261 gcaaggagtt tcatcctgat aaaggaggag atgaagaaaa aatgaagaaa atgaatactc
7321 tgtacaagaa aatggaagat ggagtaaaat atgctcatca acctgacttt ggaggcttct
7381 gggatgcaac tgaggtattt gcttcttcct taaatcctgg tgttgatgca atgtactgca
7441 aacaatggcc tgagtgtgca aagaaaatgt ctgctaactg catatgcttg ctgtgcttac
7501 tgaggatgaa gcatgaaaat agaaaattat acaggaaaga tccacttgtg tgggttgatt
7561 gctactgctt cgattgcttt agaatgtggt ttggacttga tctttgtgaa ggaaccttac
7621 ttctgtggtg tgacataatt ggacaaacta cctacagaga tttaaagctc taaggtaaat
7681 ataaaatttt taagtgtata atgtgttaaa ctactgattc taattgtttg tgtattttag
7741 attccaacct atggaactga tgaatgggag cagtggtgga atgcctttaa tgaggaaaac
7801 ctgttttgct cagaagaaat gccatctagt gatgatgagg ctactgctga ctctcaacat
7861 tctactcctc caaaaaagaa gagaaaggta gaagacccca aggactttcc ttcagaattg
7921 ctaagttttt tgagtcatgc tgtgtttagt aatagaactc ttgcttgctt tgctatttac
7981 accacaaagg aaaaagctgc actgctatac aagaaaatta tggaaaaata ttctgtaacc
8041 tttataagta ggcataacag ttataatcat aacatactgt tttttcttac tccacacagg
8101 catagagtgt ctgctattaa taactatgct caaaaattgt gtacctttag ctttttaatt
8161 tgtaaagggg ttaataagga atatttgatg tatagtgcct tgactagaga tccattttct
8221 gttattgagg aaagtttgcc aggtgggtta aaggagcatg attttaatcc agaagaagca
8281 gaggaaacta aacaagtgtc ctggaagctt gtaacagagt atgcaatgga aacaaaatgt
8341 gatgatgtgt tgttattgct tgggatgtac ttggaatttc agtacagttt tgaaatgtgt
8401 ttaaaatgta ttaaaaaaga acagcccagc cactataagt accatgaaaa gcattatgca
8461 aatgctgcta tatttgctga cagcaaaaac caaaaaacca tatgccaaca ggctgttgat
8521 actgttttag ctaaaaagcg ggttgatagc ctacaattaa ctagagaaca aatgttaaca
8581 aacagattta atgatctttt ggataggatg gatataatgt ttggttctac aggctctgct
8641 gacatagaag aatggatggc tggagttgct tggctacact gtttgttgcc caaaatggat
8701 tcagtggtgt atgacttttt aaaatgcatg gtgtacaaca ttcctaaaaa aagatactgg
8761 ctgtttaaag gaccaattga tagtggtaaa actacattag cagctgcctc gcgcgtttcg
8821 gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt
8881 aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc
8941 ggggcgcagc catgacccag tcacgtagcg atagcggagt gtatactggc ttaactatgc
9001 ggcatcagag cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg
9061 cgtaaggaga aaataccgca tcaggcgctc ttccgcttcc tcgctcactg actcgctgcg
9121 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
9181 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
9241 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
9301 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
9361 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
9421 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
9481 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
9541 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
9601 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
9661 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt
9721 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
9781 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
9841 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
9901 gaacgaaaac tcacgttaag ggattttggt catgatgata tatttttatc ttgtgcaatg
9961 taacatcaga gattttgaga cacaacgtgg ctttgttgaa taaatcgaac ttttgctgag
10021 ttgactcccc gcgcgcgatg ggtcgaattt gctttcgaaa aaaaagcccg ctcattaggc
10081 gggctaaaaa aaagcccgct cattaggcgg gctcgaattt ctgccattca tccgcttatt
10141 atcacttatt caggcgtagc aaccaggcgt ttaagggcac caataactgc cttaaaaaaa
10201 ttacgccccg ccctgccact catcgcagta ctgttgtaat tcattaagca ttctgccgac
10261 atggaagcca tcacagacgg catgatgaac ctgaatcgcc agcggcatca gcaccttgtc
10321 gccttgcgta taatatttgc ccatagtgaa aacgggggcg aagaagttgt ccatattcgc
10381 cacgtttaaa tcaaaactgg tgaaactcac ccagggattg gctgagacga aaaacatatt
10441 ctcaataaac cctttaggga aataggccag gttttcaccg taacacgcca catcttgcga
10501 atatatgtgt agaaactgcc ggaaatcgtc gtggtattca ctccagagcg atgaaaacgt
10561 ttcagtttgc tcatggaaaa cggtgtaaca agggtgaaca ctatcccata tcaccagctc
10621 accgtctttc attgccatac gaaattccgg atgagcattc atcaggcggg caagaatgtg
10681 aataaaggcc ggataaaact tgtgcttatt tttctttacg gtctttaaaa aggccgtaat
10741 atccagctga acggtctggt tataggtaca ttgagcaact gactgaaatg cctcaaaatg
10801 ttctttacga tgccattggg atatatcaac ggtggtatat ccagtgattt ttttctccat
10861 tttagcttcc ttagctcctg aaaatctcga taactcaaaa aatacgcccg gtagtgatct
10921 tatttcatta tggtgaaagt tggaacctct tacgtgccga tcaacgtctc attttcgcca
10981 aaagttggcc cagggcttcc cggtatcaac agggacacca ggatttattt attctgcgaa
11041 gtgatcttcc gtcacaggta tttattcgaa gacgaaaggg catcgcgcgc ggggaa
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