LOCUS LT727419 9484 bp DNA circular SYN 06-FEB-2017
DEFINITION Vector pBRgHIL61, complete sequence.
ACCESSION LT727419
VERSION LT727419.1
KEYWORDS .
SOURCE Vector pBRgHIL61
ORGANISM Vector pBRgHIL61
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 9484)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..9484
/organism="Vector pBRgHIL61"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 2989."
/db_xref="taxon:1945263"
misc_feature 1..1941
/note="5FR"
regulatory 1942..2477
/note="hIL6"
/regulatory_class="promoter"
misc_feature 2478..2540
/note="5UTR"
misc_feature 2541..2559
/note="hIL6"
misc_feature 2560..2721
/note="intron"
misc_feature 2722..2912
/note="hIL6"
misc_feature 2913..3490
/note="intron"
CDS 4791..5981
/transl_table=11
/note="TET"
/protein_id="SJL88388.1"
/translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASH
YGVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAG
RIVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLH
APFLAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTV
FFIMQLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGE
KQAIIAGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQG
QLQGSLAALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSR
ATST"
rep_origin complement(7055..7457)
/note="pMB1 ori"
CDS complement(7998..8858)
/transl_table=11
/note="ampicillin resistance"
/protein_id="SJL88389.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
BASE COUNT 2359 a 2425 c 2408 g 2292 t
ORIGIN
1 ttctgacata aaaacactta gtggagggct tggaaaaagt ctagtaggag caagacgcaa
61 gctggactaa ttatctaaaa caagagacct ggtttgggga tcttaatgtt ctcaaaaaag
121 aaaattatta ttatttttca ttttgcactt tgtgccataa aacattttca acaaaacata
181 gaatctcatt tcttttgagg gaaaatgatt gggagaccag ctcattgctg gcacagaggc
241 ctggttcatt cataattcct tcataggcaa gacaccaggt gaaccgatat agccgagctg
301 gaagagctct ccaaggcaga gactctgagc caaggaatgt tcaaagagct agcatgtatt
361 gtgggattac tatgcgccag gaatttttta cactgcatca cgttccatct tcacaacagc
421 cctagaaagg aagaactatt attacccccg ttttataggt gaataaacaa gggcacaggt
481 ccttgatgta acagccagga tcaaacagct gggaagacga gaaaaccttt cccaggctag
541 gataacagag gatttggttg aaaatacagg caattaggtg ctacctctgg gaaaaggggc
601 caggagagga aggagacact tttccctgca tgccctgatg tcctatttga acattttatc
661 atgaacacga acttcctatt taaaaaacac tttttattga aaagataaat ctgtgtgttg
721 tattgtgtca ctcagttcaa gtacttgaaa tttattgaat tgtattttct aaaaaataga
781 tagttgagta aaagcaagct cacattacat agacggatca cagtgcacgg ctgcggagct
841 gggagcagtg gcttcgtttc atgcaggaaa gagaacttgg ttcaggagtg tctacgttgc
901 ttaagacagg agagcactaa aaatgaaacc atccagccat cctcccccat tttcattttc
961 acaccaaaga atcccaccgc ggcagaggac caccgtctct gtttagacaa tcggtgaaga
1021 atggatgacc tcactttccc caacaggcgg gtcctgaaat gttatgcacg aaacaaaact
1081 tgagtaaatg cccaacagag gtcactgttt tatcgatctt gaagagatct cttcttagca
1141 aagcaaagaa accgattgtg aaggtaacac catgtttggt aaataagtgt tttggtgttg
1201 tgcaagggtc tggtttcagc ctgaagccat ctcagagctg tctgggtctc tggagactgg
1261 agggacaacc tagtctagag cccatttgca tgagaccaag gatcctcctg caagagacac
1321 catcctgagg gaagagggct tctgaaccag cttgacccaa taagaaattc ttgggtgccg
1381 acgcggaagc agattcagag cctagagccg tgcctgcgtc cgtagtttcc ttctagcttc
1441 ttttgatttc aaatcaagac ttacagggag agggagcgat aaacacaaac tctgcaagat
1501 gccacaaggt cctcctttga catccccaac aaagaggtga gtagtattct ccccctttct
1561 gccctgaacc aagtgggctt cagtaatttc agggctccag gagacctggg gcccatgcag
1621 gtgccccagt gaaacagtgg tgaagagact cagtggcaat ggggagagca ctggcagcac
1681 aaggcaaacc tctggcacag agagcaaagt cctcactggg aggattccca aggggtcact
1741 tgggagaggg cagggcagca gccaacctcc tctaagtggg ctgaagcagg tgaagaaagt
1801 ggcagaagcc acgcggtggc aaaaaggagt cacacactcc acctggagac gccttgaagt
1861 aactgcacga aatttgagga tggccaggca gttctacaac agccgctcac agggagagcc
1921 agaacacaga agaactcaga tgactggtag tattaccttc ttcataatcc caggcttggg
1981 gggctgcgat ggagtcagag gaaactcagt tcagaacatc tttggttttt acaaatacaa
2041 attaactgga acgctaaatt ctagcctgtt aatctggtca ctgaaaaaaa attttttttt
2101 tttcaaaaaa catagcttta gcttattttt tttctctttg taaaacttcg tgcatgactt
2161 cagctttact ctttgtcaag acatgccaaa gtgctgagtc actaataaaa gaaaaaaaga
2221 aagtaaagga agagtggttc tgcttcttag cgctagcctc aatgacgacc taagctgcac
2281 ttttccccct agttgtgtct tgcgatgcta aaggacgtca cattgcacaa tcttaataag
2341 gtttccaatc agccccaccc gctctggccc caccctcacc ctccaacaaa gatttatcaa
2401 atgtgggatt ttcccatgag tctcaatatt agagtctcaa cccccaataa atataggact
2461 ggagatgtct gaggctcatt ctgccctcga gcccaccggg aacgaaagag aagctctatc
2521 tcccctccag gagcccagct atgaactcct tctccacaag taagtgcagg aaatccttag
2581 ccctggaact gccagcggcg gtcgagccct gtgtgaggga ggggtgtgtg gcccagggag
2641 ggctggcggg cggccagcag cagaggcagg ctcccagctg tgctgtcagc tcacccctgc
2701 gctcgctccc ctccggcaca ggcgccttcg gtccagttgc cttctccctg gggctgctcc
2761 tggtgttgcc tgctgccttc cctgccccag tacccccagg agaagattcc aaagatgtag
2821 ccgccccaca cagacagcca ctcacctctt cagaacgaat tgacaaacaa attcggtaca
2881 tcctcgacgg catctcagcc ctgagaaagg aggtgggtag gcttggcgat ggggttgaag
2941 ggcccggtgc gcatgcgttc cccttgcccc tgcgtgtggc cgggggctgc ctgcattagg
3001 aggtctttgc tgggttctag agcactgtag atttgaggcc aacggggccg actagactga
3061 cttctgtatt tatcctttgc tggtgtcagg aagttccttt cctttctgga aaatgcagaa
3121 tgggtctgaa atccatgccc acctttggca tgagctgagg gttattgctt ctcagggctt
3181 ccttttccct ttccaaaaaa ttaggtctgt gaagctcctt tttgtccccc gggctttgga
3241 aggactagaa aagtgccacc tgaaaggcat gttcagcttc tcagagcagt tgcagtactt
3301 tttggttatg taaactcaat ggctaggatt cctcaaagcc attccagcta agattcatac
3361 ctcagagccc accaaagtgg caaatcataa ataggttaaa gcatctcccc actttcaatg
3421 caaggtattt tggtcctgtt tggtagaaag aaaagaacac aggaggggag attgggagcc
3481 cacactcgaa ttccgtatgg caatgaaaga cggtgagctg gtgatatggg atagtgttca
3541 cccttgttac accgttttcc atgagcaaac tgaaacgttt tcatcgctct ggagtgaata
3601 ccacgacgat ttccggcagt ttctacacat atattcgcaa gatgtggcgt gttacggtga
3661 aaacctggcc tatttcccta aagggtttat tgagaatatg tttttcgtct cagccaatcc
3721 ctgggtgagt ttcaccagtt ttgatttaaa cgtggccaat atggacaact tcttcgcccc
3781 cgttttcacc atgggcaaat attatacgca aggcgacaag gtgctgatgc cgctggcgat
3841 tcaggttcat catgccgttt gtgatggctt ccatgtcggc agaatgctta atgaattaca
3901 acagtactgc gatgagtggc agggcggggc gtaatttttt taaggcagtt attggtgccc
3961 ttaaacgcct ggtgctacgc ctgaataagt gataataagc ggatgaatgg cagaaattcg
4021 aaagcaaatt cgacccggtc gtcggttcag ggcagggtcg ttaaatagcc gcttatgtct
4081 attgctggtt taccggttta ttgactaccg gaagcagtgt gaccgtgtgc ttctcaaatg
4141 cctgaggcca gtttgctcag gctctccccg tggaggtaat aattgacgat atgatcattt
4201 attctgcctc ccagagcctg ataaaaacgg tgaatccgtt agcgaggtgc cgccggcttc
4261 cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag
4321 gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata
4381 aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga
4441 tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc
4501 gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg
4561 cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc
4621 ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg
4681 caagattccg aataccgcaa gcgacaggcc atgtttgaca gcttatcatc gataagcttt
4741 aatgcggtag tttatcacag ttaaattgct aacgcagtca ggcaccgtgt atgaaatcta
4801 acaatgcgct catcgtcatc ctcggcaccg tcaccctgga tgctgtaggc ataggcttgg
4861 ttatgccggt actgccgggc ctcttgcggg atatcgtcca ttccgacagc atcgccagtc
4921 actatggcgt gctgctagcg ctatatgcgt tgatgcaatt tctatgcgca cccgttctcg
4981 gagcactgtc cgaccgcttt ggccgccgcc cagtcctgct cgcttcgcta cttggagcca
5041 ctatcgacta cgcgatcatg gcgaccacac ccgtcctgtg gatcctctac gccggacgca
5101 tcgtggccgg catcaccggc gccacaggtg cggttgctgg cgcctatatc gccgacatca
5161 ccgatgggga agatcgggct cgccacttcg ggctcatgag cgcttgtttc ggcgtgggta
5221 tggtggcagg ccccgtggcc gggggactgt tgggcgccat ctccttgcat gcaccattcc
5281 ttgcggcggc ggtgctcaac ggcctcaacc tactactggg ctgcttccta atgcaggagt
5341 cgcataaggg agagcgtcga ccgatgccct tgagagcctt caacccagtc agctccttcc
5401 ggtgggcgcg gggcatgact atcgtcgccg cacttatgac tgtcttcttt atcatgcaac
5461 tcgtaggaca ggtgccggca gcgctctggg tcattttcgg cgaggaccgc tttcgctgga
5521 gcgcgacgat gatcggcctg tcgcttgcgg tattcggaat cttgcacgcc ctcgctcaag
5581 ccttcgtcac tggtcccgcc accaaacgtt tcggcgagaa gcaggccatt atcgccggca
5641 tggcggccga cgcgctgggc tacgtcttgc tggcgttcgc gacgcgaggc tggatggcct
5701 tccccattat gattcttctc gcttccggcg gcatcgggat gcccgcgttg caggccatgc
5761 tgtccaggca ggtagatgac gaccatcagg gacagcttca aggatcgctc gcggctctta
5821 ccagcctaac ttcgatcact ggaccgctga tcgtcacggc gatttatgcc gcctcggcga
5881 gcacatggaa cgggttggca tggattgtag gcgccgccct ataccttgtc tgcctccccg
5941 cgttgcgtcg cggtgcatgg agccgggcca cctcgacctg aatggaagcc ggcggcacct
6001 cgctaacgga ttcaccactc caagaattgg agccaatcaa ttcttgcgga gaactgtgaa
6061 tgcgcaaacc aacccttggc agaacatatc catcgcgtcc gccatctcca gcagccgcac
6121 gcggcgcatc tcgggcagcg ttgggtcctg gccacgggtg cgcatgatcg tgctcctgtc
6181 gttgaggacc cggctaggct ggcggggttg ccttactggt tagcagaatg aatcaccgat
6241 acgcgagcga acgtgaagcg actgctgctg caaaacgtct gcgacctgag caacaacatg
6301 aatggtcttc ggtttccgtg tttcgtaaag tctggaaacg cggaagtcag cgccctgcac
6361 cattatgttc cggatctgca tcgcaggatg ctgctggcta ccctgtggaa cacctacatc
6421 tgtattaacg aagcgctggc attgaccctg agtgattttt ctctggtccc gccgcatcca
6481 taccgccagt tgtttaccct cacaacgttc cagtaaccgg gcatgttcat catcagtaac
6541 ccgtatcgtg agcatcctct ctcgtttcat cggtatcatt acccccatga acagaaatcc
6601 cccttacacg gaggcatcag tgaccaaaca ggaaaaaacc gcccttaaca tggcccgctt
6661 tatcagaagc cagacattaa cgcttctgga gaaactcaac gagctggacg cggatgaaca
6721 ggcagacatc tgtgaatcgc ttcacgacca cgctgatgag ctttaccgca gctgcctcgc
6781 gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc
6841 ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg
6901 cgggtgtcgg ggcgcagcca tgacccagtc acgtagcgat agcggagtgt atactggctt
6961 aactatgcgg catcagagca gattgtactg agagtgcacc atatgcggtg tgaaataccg
7021 cacagatgcg taaggagaaa ataccgcatc aggcgctctt ccgcttcctc gctcactgac
7081 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
7141 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
7201 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
7261 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
7321 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
7381 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
7441 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
7501 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
7561 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
7621 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg
7681 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
7741 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
7801 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
7861 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
7921 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
7981 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
8041 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
8101 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
8161 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
8221 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
8281 gttaatagtt tgcgcaacgt tgttgccatt gctgcaggca tcgtggtgtc acgctcgtcg
8341 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
8401 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
8461 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
8521 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
8581 atgcggcgac cgagttgctc ttgcccggcg tcaacacggg ataataccgc gccacatagc
8641 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
8701 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
8761 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
8821 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
8881 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
8941 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa
9001 accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtctt
9061 cgaataaata cctgtgacgg aagatcactt cgcagaataa ataaatcctg gtgtccctgt
9121 tgataccggg aagccctggg ccaacttttg gcgaaaatga gacgttgatc ggcacgtaag
9181 aggttccaac tttcaccata atgaaataag atcactaccg ggcgtatttt ttgagttatc
9241 gagattttca ggagctaagg aagctaaaat ggagaaaaaa atcactggat ataccaccgt
9301 tgatatatcc caatggcatc gtaaagaaca ttttgaggca tttcagtcag ttgctcaatg
9361 tacctataac cagaccgttc agctggatat tacggccttt ttaaagaccg taaagaaaaa
9421 taagcacaag ttttatccgg cctttattca cattcttgcc cgcctgatga atgctcatcc
9481 ggaa
//