LOCUS LT727409 7937 bp DNA circular SYN 06-FEB-2017
DEFINITION Mammalian expression vector pCAGGShPARP, complete sequence.
ACCESSION LT727409
VERSION LT727409.1
KEYWORDS .
SOURCE Mammalian expression vector pCAGGShPARP
ORGANISM Mammalian expression vector pCAGGShPARP
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 7937)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..7937
/organism="Mammalian expression vector pCAGGShPARP"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 3828."
/db_xref="taxon:1944873"
CDS 94..3138
/transl_table=11
/note="hPARP"
/protein_id="SJL88367.1"
/translation="MAESSDKLYRVEYAKSGRASCKKCSESIPKDSLRMAIMVQSPMF
DGKVPHWYHFSCFWKVGHSIRHPDVEVDGFSELRWDDQQKVKKTAEAGGVTGKGQDGI
GSKAEKTLGDFAAEYAKSNRSTCKGCMEKIEKGQVRLSKKMVDPEKPQLGMIDRWYHP
GCFVKNREELGFRPEYSASQLKGFSLLATEDKEALKKQLPGVKSEGKRKGDEVDGVDE
VAKKKSKKEKDKDSKLEKALKAQNDLIWNIKDELKKVCSTNDLKELLIFNKQQVPSGE
SAILDRVADGMVFGALLPCEECSGQLVFKSDAYYCTGDVTAWTKCMVKTQTPNRKEWV
TPKEFREISYLKKLKVKKQDRIFPPETSASVAATPPPSTASAPAAVNSSASADKPLSN
MKILTLGKLSRNKDEVKAMIEKLGGKLTGTANKASLCISTKKEVEKMNKKMEEVKEAN
IRVVSEDFLQDVSASTKSLQELFLAHILSPWGAEVKAEPVEVVAPRGKSGAALSKKSK
GQVKEEGINKSEKRMKLTLKGGAAVDPDSGLEHSAHVLEKGGKVFSATLGLVDIVKGT
NSYYKLQLLEDDKENRYWIFRSWGRVGTVIGSNKLEQMPSKEDAIEHFMKLYEEKTGN
AWHSKNFTKYPKKFYPLEIDYGQDEEAVKKLTVNPGTKSKLPKPVQDLIKMIFDVESM
KKAMVEYEIDLQKMPLGKLSKRQIQAAYSILSEVQQAVSQGSSDSQILDLSNRFYTLI
PHDFGMKKPPLLNNADSVQAKVEMLDNLLDIEVAYSLLRGGSDDSSKDPIDVNYEKLK
TDIKVVDRDSEEAEIIRKYVKNTHATTHNAYDLEVIDIFKIEREGECQRYKPFKQLHN
RRLLWHGSRTTNFAGILSQGLRIAPPEAPVTGYMFGKGIYFADMVSKSANYCHTSQGD
PIGLILLGEVALGNMYELKHASHISKLPKGKHSVKGLGKTTPDPSANISLDGVDVPLG
TGISSGVNDTSLLYNEYIVYDIAQVNLKYLLKLKFNFKTSLW"
misc_feature 3139..3181
/note="3UTR"
regulatory 3227..3318
/note="polyA"
/regulatory_class="terminator"
misc_feature 3319..3675
/note="3FR"
regulatory complement(3753..3835)
/note="lac"
/regulatory_class="promoter"
rep_origin 3977..4052
/note="SV40 ori"
regulatory 4083..4157
/note="polyA"
/regulatory_class="terminator"
regulatory complement(4083..4157)
/note="polyA"
/regulatory_class="terminator"
rep_origin complement(4272..4673)
/note="pMB1 ori"
CDS complement(5214..6074)
/transl_table=11
/note="ampicillin resistance"
/protein_id="SJL88368.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
misc_feature 6210..6587
/note="hCMV"
regulatory 6593..7934
/note="AG"
/regulatory_class="promoter"
misc_feature 6962..7886
/note="intron"
BASE COUNT 1884 a 2005 c 2221 g 1827 t
ORIGIN
1 ttcctcgagg aattcactcc tcaggtgcag gctgcctatc agaaggtggt ggctggtgtg
61 gggggatcct ctagagtcga cctgcagggg aggatggcgg agtcttcgga taagctctat
121 cgagtcgagt acgccaagag cgggcgcgcc tcttgcaaga aatgcagcga gagcatcccc
181 aaggactcgc tccggatggc catcatggtg cagtcgccca tgtttgatgg aaaagtccca
241 cactggtacc acttctcctg cttctggaag gtgggccact ccatccggca ccctgacgtt
301 gaggtggatg ggttctctga gcttcggtgg gatgaccagc agaaagtcaa gaagacagcg
361 gaagctggag gagtgacagg caaaggccag gatggaattg gtagcaaggc agagaagact
421 ctgggtgact ttgcagcaga gtatgccaag tccaacagaa gtacgtgcaa ggggtgtatg
481 gagaagatag aaaagggcca ggtgcgcctg tccaagaaga tggtggaccc ggagaagcca
541 cagctaggca tgattgaccg ctggtaccat ccaggctgct ttgtcaagaa cagggaggag
601 ctgggtttcc ggcccgagta cagtgcgagt cagctcaagg gcttcagcct ccttgctaca
661 gaggataaag aagccctgaa gaagcagctc ccaggagtca agagtgaagg aaagagaaaa
721 ggcgatgagg tggatggagt ggatgaagtg gcgaagaaga aatctaaaaa agaaaaagac
781 aaggatagta agcttgaaaa agccctaaag gctcagaacg acctgatctg gaacatcaag
841 gacgagctaa agaaagtgtg ttcaactaat gacctgaagg agctactcat cttcaacaag
901 cagcaagtgc cttctgggga gtcggcgatc ttggaccgag tagctgatgg catggtgttc
961 ggtgccctcc ttccctgcga ggaatgctcg ggtcagctgg tcttcaagag cgatgcctat
1021 tactgcactg gggacgtcac tgcctggacc aagtgtatgg tcaagacaca gacacccaac
1081 cggaaggagt gggtaacccc aaaggaattc cgagaaatct cttacctcaa gaaattgaag
1141 gttaaaaagc aggaccgtat attcccccca gaaaccagcg cctccgtggc ggccacgcct
1201 ccgccctcca cagcctcggc tcctgctgct gtgaactcct ctgcttcagc agataagcca
1261 ttatccaaca tgaagatcct gactctcggg aagctgtccc ggaacaagga tgaagtgaag
1321 gccatgattg agaaactcgg ggggaagttg acggggacgg ccaacaaggc ttccctgtgc
1381 atcagcacca aaaaggaggt ggaaaagatg aataagaaga tggaggaagt aaaggaagcc
1441 aacatccgag ttgtgtctga ggacttcctc caggacgtct ccgcctccac caagagcctt
1501 caggagttgt tcttagcgca catcttgtcc ccttgggggg cagaggtgaa ggcagagcct
1561 gttgaagttg tggccccaag agggaagtca ggggctgcgc tctccaaaaa aagcaagggc
1621 caggtcaagg aggaaggtat caacaaatct gaaaagagaa tgaaattaac tcttaaagga
1681 ggagcagctg tggatcctga ttctggactg gaacactctg cgcatgtcct ggagaaaggt
1741 gggaaggtct tcagtgccac ccttggcctg gtggacatcg ttaaaggaac caactcctac
1801 tacaagctgc agcttctgga ggacgacaag gaaaacaggt attggatatt caggtcctgg
1861 ggccgtgtgg gtacggtgat cggtagcaac aaactggaac agatgccgtc caaggaggat
1921 gccattgagc acttcatgaa attatatgaa gaaaaaaccg ggaacgcttg gcactccaaa
1981 aatttcacga agtatcccaa aaagttctac cccctggaga ttgactatgg ccaggatgaa
2041 gaggcagtga agaagctgac agtaaatcct ggcaccaagt ccaagctccc caagccagtt
2101 caggacctca tcaagatgat ctttgatgtg gaaagtatga agaaagccat ggtggagtat
2161 gagatcgacc ttcagaagat gcccttgggg aagctgagca aaaggcagat ccaggccgca
2221 tactccatcc tcagtgaggt ccagcaggcg gtgtctcagg gcagcagcga ctctcagatc
2281 ctggatctct caaatcgctt ttacaccctg atcccccacg actttgggat gaagaagcct
2341 ccgctcctga acaatgcaga cagtgtgcag gccaaggtgg aaatgcttga caacctgctg
2401 gacatcgagg tggcctacag tctgctcagg ggagggtctg atgatagcag caaggatccc
2461 atcgatgtca actatgagaa gctcaaaact gacattaagg tggttgacag agattctgaa
2521 gaagccgaga tcatcaggaa gtatgttaag aacactcatg caaccacaca caatgcgtat
2581 gacttggaag tcatcgatat ctttaagata gagcgtgaag gcgaatgcca gcgttacaag
2641 ccctttaagc agcttcataa ccgaagattg ctgtggcacg ggtccaggac caccaacttt
2701 gctgggatcc tgtcccaggg tcttcggata gccccgcctg aagcgcccgt gacaggctac
2761 atgtttggta aagggatcta tttcgctgac atggtctcca agagtgccaa ctactgccat
2821 acgtctcagg gagacccaat aggcttaatc ctgttgggag aagttgccct tggaaacatg
2881 tatgaactga agcacgcttc acatatcagc aagttaccca agggcaagca cagtgtcaaa
2941 ggtttgggca aaactacccc tgatccttca gctaacatta gtctggatgg tgtagacgtt
3001 cctcttggga ccgggatttc atctggtgtg aatgacacct ctctactata taacgagtac
3061 attgtctatg atattgctca ggtaaatctg aagtatctgc tgaaactgaa attcaatttt
3121 aagacctccc tgtggtaatt gggagaggta gccgagtcac acccggtggc tctggtatga
3181 attcgagctc ggtaccccca atgccctggc tcacaaatac cactgagatc tttttccctc
3241 tgccaaaaat tatggggaca tcatgaagcc ccttgagcat ctgacttctg gctaataaag
3301 gaaatttatt ttcattgcaa tagtgtgttg gaattttttg tgtctctcac tcggaaggac
3361 atatgggagg gcaaatcatt taaaacatca gaatgagtat ttggtttaga gtttggcaac
3421 atatgcccat atgctggctg ccatgaacaa aggttggcta taaagaggtc atcagtatat
3481 gaaacagccc cctgctgtcc attccttatt ccatagaaaa gccttgactt gaggttagat
3541 tttttttata ttttgttttg tgttattttt ttctttaaca tccctaaaat tttccttaca
3601 tgttttacta gccagatttt tcctcctctc ctgactactc ccagtcatag ctgtccctct
3661 tctcttatgg agatccctcg acctgcagcc caagcttggc gtaatcatgg tcatagctgt
3721 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
3781 agtgtaaagc ctgggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact
3841 gcccgctttc cagtcgggaa acctgtcgtg ccagcggatc cgcatctcaa ttagtcagca
3901 accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat
3961 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc
4021 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag
4081 ctaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
4141 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
4201 cttatcatgt ctggatccgc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
4261 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
4321 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggat
4381 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
4441 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
4501 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
4561 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
4621 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
4681 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
4741 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
4801 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
4861 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg
4921 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
4981 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
5041 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
5101 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
5161 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
5221 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
5281 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
5341 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
5401 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
5461 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
5521 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
5581 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
5641 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
5701 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
5761 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
5821 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
5881 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
5941 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
6001 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
6061 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
6121 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
6181 acatttcccc gaaaagtgcc acctgggtcg acattgatta ttgactagtt attaatagta
6241 atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac
6301 ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac
6361 gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt
6421 acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat
6481 tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga
6541 ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg tcgaggtgag
6601 ccccacgttc tgcttcactc tccccatctc ccccccctcc ccacccccaa ttttgtattt
6661 atttattttt taattatttt gtgcagcgat gggggcgggg gggggggggg ggcccccccc
6721 aggcggggcg gggcggggcg aggggcgggg cggggcgagg cggaaaggtg cggcggcagc
6781 caatcagagc ggcgcgctcc gaaagtttcc ttttatggcg aggcggcggc ggcggcggcc
6841 ctataaaaag cgaagcgcgc ggcgggcggg agtcgttgcg cgctgccttc cccccgtgcc
6901 ccgctccgcc gccgcctcgc gccgcccgcc ccggctctga ctgaccgcgt tactcccaca
6961 ggtgagcggg cgggacggcc cttctcctcc gggctgtaat tagcgcttgg tttaatgacg
7021 gcttgtttct tttctgtggc tgcgtgaaag ccttgagggg ctccgggagg gccctttgtg
7081 cggggggagc ggctcggggg gtgcgtgcgt gtgtgtgtgc gtggggagcg ccgcgtgcgg
7141 ctccgcgctg cccggcggct gtgagcgctg cgggcgcggc gcggggcttt gtgcgctccg
7201 cagtgtgcgc gaggggagcg cggccggggg cggtgccccg cggtgcgggg ggggctgcga
7261 ggggaacaaa ggctgcgtgc ggggtgtgtg cgtggggggg tgagcagggg gtgtgggcgc
7321 gtcggtcggg ctgcaacccc ccctgcaccc ccctccccga gttgctgagc acggcccggc
7381 ttcgggtgcg gggctccgta cggggcgtgg cgcggggctc gccgtgccgg gcggggggtg
7441 gcggcaggtg ggggtgccgg gcggggcggg gccgcctcgg gccggggagg gctcggggga
7501 aggggcgcgg cggcccccgg agcgccggcg gctgtcgagg cgcggcgagc cgcagccatt
7561 gccttttatg gtaatcgtgc gagagggcgc agggacttcc tttgtcccaa atctgtgcgg
7621 agccgaaatc tgggaggcgc cgccgcaccc cctctagcgg gcgcggggcg aagcggtgcg
7681 gcgccggcag gaaggaaatg ggcggggagg gccttcgtgc gtcgccgcgc cgccgtcccc
7741 ttctccctct ccagcctcgg ggctgtccgc ggggggacgg ctgccttcgg gggggacggg
7801 gcagggcggg gttcggcttc tggcgtgtga ccggcggctc tagagcctct gctaaccatg
7861 ttcatgcctt cttctttttc ctacagctcc tgggcaacgt gctggttatt gtgctgtctc
7921 atcattttgg caaagaa
//