LOCUS LT727407 8408 bp DNA circular SYN 06-FEB-2017
DEFINITION Mammalian expression vector pCAGGS-mCASP-2a, complete sequence.
ACCESSION LT727407
VERSION LT727407.1
KEYWORDS .
SOURCE Mammalian expression vector pCAGGS-mCASP-2a
ORGANISM Mammalian expression vector pCAGGS-mCASP-2a
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 8408)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..8408
/organism="Mammalian expression vector pCAGGS-mCASP-2a"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 3825."
/db_xref="taxon:1944882"
misc_feature complement(182..2200)
/note="3UTR"
CDS complement(2201..3559)
/transl_table=11
/note="CASP2"
/protein_id="SJL88363.1"
/translation="MAAPSGRSQSSLHRKGLMAADRRSRILAVCGMHPDHQETLKKNR
VVLAKQLLLSELLEHLLEKDIITLEMRELIQAKGGSFSQNVELLNLLPKRGPQAFDAF
CEALRETRQGHLEDLLLTTLSDIQHVLPPLSCDYDTSLPFSVCESCPPHKQLRLSTDA
TEHSLDNGDGPPCLLVKPCTPEFYQAHYQLAYRLQSQPRGLALVLSNVHFTGEKDLEF
RSGGDVDHTTLVTLFKLLGYNVHVLHDQTAQEMQEKLQNFAQLPAHRVTDSCVVALLS
HGVEGGIYGVDGKLLQLQEVFRLFDNANCPSLQNKPKMFFIQACRGDETDRGVDQQDG
KNHTQSPGCEESDAGKEELMKMRLPTRSDMICGYACLKGNAAMRNTKRGSWYIEALTQ
VFSERACDMHVADMLVKVNALIKEREGYAPGTEFHRCKEMSEYCSTLCQQLYLFPGYP
PT"
misc_feature complement(3560..3644)
/note="5UTR"
regulatory 3698..3789
/note="polyA"
/regulatory_class="terminator"
misc_feature 3790..4146
/note="3FR"
regulatory complement(4223..4306)
/note="lac"
/regulatory_class="promoter"
rep_origin 4448..4523
/note="SV40 ori"
regulatory 4554..4628
/note="polyA"
/regulatory_class="terminator"
regulatory complement(4554..4628)
/note="polyA"
/regulatory_class="terminator"
rep_origin complement(4743..5144)
/note="pMB1 ori"
CDS complement(5685..6545)
/transl_table=11
/note="ampicillin resistance"
/protein_id="SJL88364.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
misc_feature 6681..7058
/note="hCMV"
regulatory 7064..8405
/note="AG"
/regulatory_class="promoter"
misc_feature 7433..8357
/note="intron"
BASE COUNT 2102 a 2123 c 2189 g 1994 t
ORIGIN
1 ttcctcgagg aattcactcc tcaggtgcag gctgcctatc agaaggtggt ggctggtgtg
61 gcgcgtttgg aatcactaca gggatgttta ataccactac aatggatgat gtatataact
121 atctattcga tgatgaagat accccaccaa acccaaaaaa agagatcgaa ttcggcacga
181 gatcaacatt tatttggcac ctgatggcaa tacaaaatct tggcagtggg aagtgtgaag
241 attctaccac ttatgcatcc tatactgacc caaaaggcag aatttggcag caagtcttca
301 aaccacttgg ttcatacaac aaacattagt tgagcacctc aactaccata ggcctcaaat
361 ttgctgggca ctatggagat gtgaaagaag ttgtctcaca cgtgatatgt agaaaatata
421 aaaaaacaac aacaaaacag tggataaaaa taaaacacct ggaaaaaaaa acccatatac
481 taattgaact gtacactata atagcaattt agaaactgca aaaggtaggc tctaactaag
541 gttagctgct ggtctccttc ctttactcag gaaacattca tgaagaccta gtaagtgcca
601 gttactggat atcagtaagt aagaagcaca gttcccactc tcttggagca ttcagcctca
661 gagggaacaa gtgaaacaag caaaagtgcc attacagcct gcagtaagca caacagagac
721 caacatgatg ccctcataag gaggcacagt agacagcatg ggcagcatgg tcaagaggcc
781 tccgccagac tcaaaaatca gagactagcc aaaggagagt tcagaacacc tgcaagacgg
841 agatgaggac ctaagagggg ctggagcata gtgagaaatc accatttaat gagatccaaa
901 gggaaggtat gagccagatc tcgtaagctg ggtgtaagaa tttgcatttt agatgaagcg
961 taacaggaag ctagtgttct aagcaagaga atgacatcac cagatatttt aaaaaccaaa
1021 ctactctgtt acatggagaa taaattggaa gagaggaaaa aatggaaatg cagaaggttt
1081 tcaagaataa gcaagtttca taagagcaga gacaagtgct tctaggttat atcacatatg
1141 gtactatccc cactaaaaca cagagaaaca atgagaaggg agacgctgcc acagaaaacc
1201 aaagaagcct cacgctgaag cagaaggcag aaagaataag caactacagt acagcgtaag
1261 tggcaagaaa catcctacaa aggacggctt cacagacttg gcagaagcag cgaatggtga
1321 aggtaaagag gggaaccagt acaaagcaag agagaccctg gaggccagcc gtcaagtaca
1381 gtgtaagtgc atgcagaagc cacagtaagg aaggagagac atcctgatga cgggtgatag
1441 tgtgagacac agcacactca gctgtcactg cattttcatg tagttatgcc cgataaggtt
1501 cattcagtga cagttcaaac aaagcatgac cagggaaatt tctatgactt ccagtgtcat
1561 ggcatacaga ccctaggctc tgttcaaagg ctgggtgcac tgaacacaaa ggaaccaatg
1621 gaaaggctga gggactgtcc ttcacctagg gcctgaactt aaaggtatgc atagaaactt
1681 cgcaagtagt aggatctcat ctgtcccttc atgacagatg aaaacaccta cattaaattg
1741 ggacatcttc caaatctcca ctgtgggaaa aatgaggttg tagatatgga aaaaaaagga
1801 gatgatctct gagactcctt aactgattta ggaaaaaaaa aaaaagctat gatccaagta
1861 ctcctaagat gccttgggaa caaaaatatc acaaacattg agaaatccaa atgccctctt
1921 cccacaataa tactgtgttc acaatgtcat tttctaggct gtagctaaaa tgcttctggt
1981 aacacatcca ttcagagctg gctctatagg tagagaacac gacaccggag gagccatcct
2041 tatgtttcat acccaaagac agagatggct gacaggaaac aagcctggga gaatctcata
2101 tccctgagag ggtagacaga acaaaaacca ttctgaagag aagtctccat tgtgccccca
2161 gtggtccagt ggcctccaac agcaggaata gcaggcggca tcacgtgggt gggtagcctg
2221 ggaacaggta gagttgctgg cacagagtac tacagtactc agacatctcc ttgcatcggt
2281 ggaattctgt gccaggggca tagccttcac gctccttgat aagggcattc accttaacaa
2341 gcatgtcggc cacgtgcatg tcacaagctc tttcagagaa cacctgagtg agggcctcaa
2401 tgtaccagga accccgtttg gtgttccgca tggcagcatt acctttaagg caagcatagc
2461 cacatatcat gtctgagcga gtaggcagtc tcatcttcat caactcctct ttgccagcat
2521 cactctcctc acatccaggg gattgtgtgt ggttctttcc atcttgctgg tcgacacctc
2581 tatctgtctc atctccacga catgcttgga tgaagaacat ttttggcttg ttctgtagac
2641 ttggacagtt agcattgtca aaaagtcgaa aaacctcttg gagctgaagc agtttgccat
2701 ctacaccata gatgccacct tccacaccat gtgagaggag tgccactacg caggagtctg
2761 tgacccggtg tgcaggtaac tgtgcaaaat tctgaagttt ctcttgcatt tcctgtgcgg
2821 tctggtcatg tagcacatgg acattgtagc ccaaaagctt gaagagggtg actagagtag
2881 tgtggtccac atcccctcca gagcggaatt ccaggtcttt ctctccagtg aagtgcacat
2941 tgctcagcac cagtgccaag ccacggggct gagattgcaa cctataggcc agctggtagt
3001 gtgcctggta aaactcagga gtgcatggct tcaccagaag acagggagga ccatcaccat
3061 tatctaagga gtgttccgta gcatctgtgg ataggcggag ctgcttgtga ggagggcagg
3121 actcacacac cgagaaaggg agacttgtgt catagtcaca gctcaacggt gggagtacgt
3181 gctgaatatc tgagagggtt gtgagcagta agtcttccaa gtgaccctgc ctggtctccc
3241 gcagggcttc acagaaggca tcaaaagcct ggggtcctct ctttggcagc aggttgagga
3301 gttccacatt ctggctgaaa ctgccccctt tggcctggat gagctccctc atttccaaag
3361 tgataatgtc cttctctagg aggtgttcta acagctcgct cagcagcagc tgcttggcca
3421 gcaccactcg attctttttc agtgtttcct ggtggtcagg atgcattcca cacactgcca
3481 aaatcctgct cctcctgtca gccgccatca gccccttcct gtgcagggag gactgcgacc
3541 tcccgctcgg cgccgccatt tccagctttt cccgggctgc acacgcacca cccacaaaac
3601 aaacaaacgc tgggccgcgc cagcccccgc atccctcccc cagccctcgt gccgaattcc
3661 cggggatccc aatgccctgg ctcacaaata ccactgagat ctttttccct ctgccaaaaa
3721 ttatggggac atcatgaagc cccttgagca tctgacttct ggctaataaa ggaaatttat
3781 tttcattgca atagtgtgtt ggaatttttt gtgtctctca ctcggaagga catatgggag
3841 ggcaaatcat ttaaaacatc agaatgagta tttggtttag agtttggcaa catatgccca
3901 tatgctggct gccatgaaca aaggttggct ataaagaggt catcagtata tgaaacagcc
3961 ccctgctgtc cattccttat tccatagaaa agccttgact tgaggttaga ttttttttat
4021 attttgtttt gtgttatttt tttctttaac atccctaaaa ttttccttac atgttttact
4081 agccagattt ttcctcctct cctgactact cccagtcata gctgtccctc ttctcttatg
4141 gagatccctc gacctgcagc ccaagcttgg cgtaatcatg gtcatagctg tttcctgtgt
4201 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag
4261 cctgggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt
4321 ccagtcggga aacctgtcgt gccagcggat ccgcatctca attagtcagc aaccatagtc
4381 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
4441 catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta
4501 ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctaacttgt
4561 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
4621 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
4681 tctggatccg ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
4741 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
4801 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcaggga taacgcagga
4861 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
4921 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
4981 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
5041 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
5101 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
5161 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
5221 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
5281 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
5341 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
5401 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
5461 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
5521 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
5581 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
5641 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
5701 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
5761 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
5821 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
5881 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
5941 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
6001 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
6061 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
6121 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
6181 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
6241 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
6301 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
6361 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
6421 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
6481 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
6541 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
6601 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
6661 cgaaaagtgc cacctgggtc gacattgatt attgactagt tattaatagt aatcaattac
6721 ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg
6781 cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc
6841 catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac
6901 tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa
6961 tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac
7021 ttggcagtac atctacgtat tagtcatcgc tattaccatg gtcgaggtga gccccacgtt
7081 ctgcttcact ctccccatct cccccccctc cccaccccca attttgtatt tatttatttt
7141 ttaattattt tgtgcagcga tgggggcggg gggggggggg gggccccccc caggcggggc
7201 ggggcggggc gaggggcggg gcggggcgag gcggaaaggt gcggcggcag ccaatcagag
7261 cggcgcgctc cgaaagtttc cttttatggc gaggcggcgg cggcggcggc cctataaaaa
7321 gcgaagcgcg cggcgggcgg gagtcgttgc gcgctgcctt ccccccgtgc cccgctccgc
7381 cgccgcctcg cgccgcccgc cccggctctg actgaccgcg ttactcccac aggtgagcgg
7441 gcgggacggc ccttctcctc cgggctgtaa ttagcgcttg gtttaatgac ggcttgtttc
7501 ttttctgtgg ctgcgtgaaa gccttgaggg gctccgggag ggccctttgt gcggggggag
7561 cggctcgggg ggtgcgtgcg tgtgtgtgtg cgtggggagc gccgcgtgcg gctccgcgct
7621 gcccggcggc tgtgagcgct gcgggcgcgg cgcggggctt tgtgcgctcc gcagtgtgcg
7681 cgaggggagc gcggccgggg gcggtgcccc gcggtgcggg gggggctgcg aggggaacaa
7741 aggctgcgtg cggggtgtgt gcgtgggggg gtgagcaggg ggtgtgggcg cgtcggtcgg
7801 gctgcaaccc cccctgcacc cccctccccg agttgctgag cacggcccgg cttcgggtgc
7861 ggggctccgt acggggcgtg gcgcggggct cgccgtgccg ggcggggggt ggcggcaggt
7921 gggggtgccg ggcggggcgg ggccgcctcg ggccggggag ggctcggggg aaggggcgcg
7981 gcggcccccg gagcgccggc ggctgtcgag gcgcggcgag ccgcagccat tgccttttat
8041 ggtaatcgtg cgagagggcg cagggacttc ctttgtccca aatctgtgcg gagccgaaat
8101 ctgggaggcg ccgccgcacc ccctctagcg ggcgcggggc gaagcggtgc ggcgccggca
8161 ggaaggaaat gggcggggag ggccttcgtg cgtcgccgcg ccgccgtccc cttctccctc
8221 tccagcctcg gggctgtccg cggggggacg gctgccttcg ggggggacgg ggcagggcgg
8281 ggttcggctt ctggcgtgtg accggcggct ctagagcctc tgctaaccat gttcatgcct
8341 tcttcttttt cctacagctc ctgggcaacg tgctggttat tgtgctgtct catcattttg
8401 gcaaagaa
//