LOCUS       LT727406                6040 bp    DNA     circular SYN 06-FEB-2017
DEFINITION  Mammalian expression vector pCAGGS-mCASP-6a, complete sequence.
ACCESSION   LT727406
VERSION     LT727406.1
KEYWORDS    .
SOURCE      Mammalian expression vector pCAGGS-mCASP-6a
  ORGANISM  Mammalian expression vector pCAGGS-mCASP-6a
            other sequences; artificial sequences; vectors.
REFERENCE   1  (bases 1 to 6040)
  AUTHORS   De Schamphelaire W.
  JOURNAL   Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
            Technologiepark 927, 9052, BELGIUM.
REFERENCE   2
  AUTHORS   De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
            Roggeman Fonseca M., Vanhoucke M., Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished.
FEATURES             Location/Qualifiers
     source          1..6040
                     /organism="Mammalian expression vector pCAGGS-mCASP-6a"
                     /mol_type="other DNA"
                     /note="BCCM/LMBP Plasmid collection (Ghent
                     University,Belgium) accession number LMBP 3824."
                     /db_xref="taxon:1944886"
     misc_feature    complement(12..385)
                     /note="3UTR"
     CDS             complement(386..1216)
                     /transl_table=11
                     /note="CASP6"
                     /protein_id="SJL88361.1"
                     /translation="MTETDGFYKSREVFDPAEQYKMDHKRRGVALIFNHERFFWHLTL
                     PERRGTNADRDNLTRRFSDLGFEVKCFNDLRAEELLLKIHEVSTSSHIDADCFICVFL
                     SHGEGNHVYAYDAKIEIQTLTGLFKGDKCQSLVGKPKIFIIQACRGSQHDVPVVPLDM
                     VDHQTDKLDNVTQVDAASVYTLPAGADFLMCYSVAEGYYSHRETVNGSWYIQDLCEML
                     ARYGSSLEFTELLTLVNRKVSQRRVDFCKDPDAIGKKQVPCFASMLTKKLHFCPKPSK
                     "
     misc_feature    complement(1217..1240)
                     /note="5UTR"
     regulatory      1330..1421
                     /note="polyA"
                     /regulatory_class="terminator"
     misc_feature    1422..1778
                     /note="3FR"
     regulatory      complement(1856..1938)
                     /note="lac"
                     /regulatory_class="promoter"
     rep_origin      2080..2155
                     /note="SV40 ori"
     regulatory      2186..2260
                     /note="polyA"
                     /regulatory_class="terminator"
     regulatory      complement(2186..2260)
                     /note="polyA"
                     /regulatory_class="terminator"
     rep_origin      complement(2375..2776)
                     /note="pMB1 ori"
     CDS             complement(3317..4177)
                     /transl_table=11
                     /note="ampicillin resistance"
                     /protein_id="SJL88362.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
     misc_feature    4313..4690
                     /note="hCMV"
     regulatory      4696..6037
                     /note="AG"
                     /regulatory_class="promoter"
     misc_feature    5065..5989
                     /note="intron"
BASE COUNT         1320 a         1566 c         1625 g         1529 t
ORIGIN      
        1 ttcggcacga ggttcttaga cattttatta cgcgaaaaca atctcagtct agagtttcaa
       61 aagtctttac aaacattata cagtaaactc atataaacag tgtcacaaaa ggggaggcaa
      121 agtagttggt atttagagta atcttacagt ttactaaaaa tgtaataaat gtagtagaca
      181 ttgatgcata gtaatttcct gaggattatt tttagccctt ccaccacgtc caacatgatt
      241 gaaaagtaac aaatataaaa cattactttt ctatggcatc tacaaaattg aagcagttta
      301 cattgatggg tgaaagtctt ccaccagctg gcctttgtat aaaaagggaa atgtgtgtga
      361 aatatatgta gcaagacaga tggccctact tgctaggttt gggacagaaa tgcagctttt
      421 tggtcagcat tgaggcgaag cagggcacct gcttcttgcc gattgcatcc gggtctttgc
      481 agaagtccac gcggcgctga gagacctttc tgttcaccag cgtgagcagc tccgtgaact
      541 ccagggaact gccgtacctc gccagcatct cacacaaatc ctgaatgtac caggagccat
      601 tcacagtttc tcggtgagag taataccctt ctgcgacaga gtagcacatg aggaagtctg
      661 cccctgccgg cagcgtgtac acggacgcag catccacctg ggtcacgttg tccagcttgt
      721 ctgtctggtg atccaccatg tccaggggaa ccacgggtac gtcatgctgg ctaccccgac
      781 aggcctggat gataaatatc ttgggttttc caaccaggct ctgacacttg tctcctttga
      841 acaagccagt caacgtctga atttcaattt tggcgtcgta tgcgtaaacg tggttgcctt
      901 ccccatggct caggaagaca cagatgaagc aatcggcatc tatgtggctt gaagtcgaca
      961 cctcgtgaat tttgagcagg agttcttctg ctctgaggtc gttaaagcat ttcacttcaa
     1021 atcctagatc tgaaaacctg cgagtcaggt tgtctctgtc tgcgttggtg ccccgcctct
     1081 ctgggagggt caggtgccaa aagaacctct cgtgattgaa gatgagggca actcctctcc
     1141 tcttgtggtc catcttgtac tgctcggctg gatcgaacac ttccctactt ttgtagaagc
     1201 catcggtttc tgtcatgttt tactttcacc tgcccgcgag ctcgtgccga attcactcct
     1261 caggtgcagg ctgcctatca gaaggtggtg gctggtgtgg ccaatgccct ggctcacaaa
     1321 taccactgag atctttttcc ctctgccaaa aattatgggg acatcatgaa gccccttgag
     1381 catctgactt ctggctaata aaggaaattt attttcattg caatagtgtg ttggaatttt
     1441 ttgtgtctct cactcggaag gacatatggg agggcaaatc atttaaaaca tcagaatgag
     1501 tatttggttt agagtttggc aacatatgcc catatgctgg ctgccatgaa caaaggttgg
     1561 ctataaagag gtcatcagta tatgaaacag ccccctgctg tccattcctt attccataga
     1621 aaagccttga cttgaggtta gatttttttt atattttgtt ttgtgttatt tttttcttta
     1681 acatccctaa aattttcctt acatgtttta ctagccagat ttttcctcct ctcctgacta
     1741 ctcccagtca tagctgtccc tcttctctta tggagatccc tcgacctgca gcccaagctt
     1801 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
     1861 caacatacga gccggaagca taaagtgtaa agcctgggtg cctaatgagt gagctaactc
     1921 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagcgg
     1981 atccgcatct caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc
     2041 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg
     2101 cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg
     2161 gaggcctagg cttttgcaaa aagctaactt gtttattgca gcttataatg gttacaaata
     2221 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
     2281 tttgtccaaa ctcatcaatg tatcttatca tgtctggatc cgctgcatta atgaatcggc
     2341 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
     2401 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     2461 cggttatcca cagaatcagg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     2521 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     2581 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     2641 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     2701 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     2761 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     2821 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     2881 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     2941 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa
     3001 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     3061 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     3121 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     3181 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     3241 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     3301 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     3361 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
     3421 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
     3481 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
     3541 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
     3601 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
     3661 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
     3721 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
     3781 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
     3841 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
     3901 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
     3961 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
     4021 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
     4081 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
     4141 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
     4201 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
     4261 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctggg tcgacattga
     4321 ttattgacta gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg
     4381 gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc
     4441 cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat
     4501 tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat
     4561 catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat
     4621 gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc
     4681 gctattacca tggtcgaggt gagccccacg ttctgcttca ctctccccat ctcccccccc
     4741 tccccacccc caattttgta tttatttatt ttttaattat tttgtgcagc gatgggggcg
     4801 gggggggggg gggggccccc cccaggcggg gcggggcggg gcgaggggcg gggcggggcg
     4861 aggcggaaag gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt tccttttatg
     4921 gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc gggagtcgtt
     4981 gcgcgctgcc ttccccccgt gccccgctcc gccgccgcct cgcgccgccc gccccggctc
     5041 tgactgaccg cgttactccc acaggtgagc gggcgggacg gcccttctcc tccgggctgt
     5101 aattagcgct tggtttaatg acggcttgtt tcttttctgt ggctgcgtga aagccttgag
     5161 gggctccggg agggcccttt gtgcgggggg agcggctcgg ggggtgcgtg cgtgtgtgtg
     5221 tgcgtgggga gcgccgcgtg cggctccgcg ctgcccggcg gctgtgagcg ctgcgggcgc
     5281 ggcgcggggc tttgtgcgct ccgcagtgtg cgcgagggga gcgcggccgg gggcggtgcc
     5341 ccgcggtgcg gggggggctg cgaggggaac aaaggctgcg tgcggggtgt gtgcgtgggg
     5401 gggtgagcag ggggtgtggg cgcgtcggtc gggctgcaac cccccctgca cccccctccc
     5461 cgagttgctg agcacggccc ggcttcgggt gcggggctcc gtacggggcg tggcgcgggg
     5521 ctcgccgtgc cgggcggggg gtggcggcag gtgggggtgc cgggcggggc ggggccgcct
     5581 cgggccgggg agggctcggg ggaaggggcg cggcggcccc cggagcgccg gcggctgtcg
     5641 aggcgcggcg agccgcagcc attgcctttt atggtaatcg tgcgagaggg cgcagggact
     5701 tcctttgtcc caaatctgtg cggagccgaa atctgggagg cgccgccgca ccccctctag
     5761 cgggcgcggg gcgaagcggt gcggcgccgg caggaaggaa atgggcgggg agggccttcg
     5821 tgcgtcgccg cgccgccgtc cccttctccc tctccagcct cggggctgtc cgcgggggga
     5881 cggctgcctt cgggggggac ggggcagggc ggggttcggc ttctggcgtg tgaccggcgg
     5941 ctctagagcc tctgctaacc atgttcatgc cttcttcttt ttcctacagc tcctgggcaa
     6001 cgtgctggtt attgtgctgt ctcatcattt tggcaaagaa
//