LOCUS LT727406 6040 bp DNA circular SYN 06-FEB-2017 DEFINITION Mammalian expression vector pCAGGS-mCASP-6a, complete sequence. ACCESSION LT727406 VERSION LT727406.1 KEYWORDS . SOURCE Mammalian expression vector pCAGGS-mCASP-6a ORGANISM Mammalian expression vector pCAGGS-mCASP-6a other sequences; artificial sequences; vectors. REFERENCE 1 (bases 1 to 6040) AUTHORS De Schamphelaire W. JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent, Technologiepark 927, 9052, BELGIUM. REFERENCE 2 AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K., Roggeman Fonseca M., Vanhoucke M., Beyaert R. TITLE BCCM/LMBP Plasmid collection JOURNAL Unpublished. FEATURES Location/Qualifiers source 1..6040 /organism="Mammalian expression vector pCAGGS-mCASP-6a" /mol_type="other DNA" /note="BCCM/LMBP Plasmid collection (Ghent University,Belgium) accession number LMBP 3824." /db_xref="taxon:1944886" misc_feature complement(12..385) /note="3UTR" CDS complement(386..1216) /transl_table=11 /note="CASP6" /protein_id="SJL88361.1" /translation="MTETDGFYKSREVFDPAEQYKMDHKRRGVALIFNHERFFWHLTL PERRGTNADRDNLTRRFSDLGFEVKCFNDLRAEELLLKIHEVSTSSHIDADCFICVFL SHGEGNHVYAYDAKIEIQTLTGLFKGDKCQSLVGKPKIFIIQACRGSQHDVPVVPLDM VDHQTDKLDNVTQVDAASVYTLPAGADFLMCYSVAEGYYSHRETVNGSWYIQDLCEML ARYGSSLEFTELLTLVNRKVSQRRVDFCKDPDAIGKKQVPCFASMLTKKLHFCPKPSK " misc_feature complement(1217..1240) /note="5UTR" regulatory 1330..1421 /note="polyA" /regulatory_class="terminator" misc_feature 1422..1778 /note="3FR" regulatory complement(1856..1938) /note="lac" /regulatory_class="promoter" rep_origin 2080..2155 /note="SV40 ori" regulatory 2186..2260 /note="polyA" /regulatory_class="terminator" regulatory complement(2186..2260) /note="polyA" /regulatory_class="terminator" rep_origin complement(2375..2776) /note="pMB1 ori" CDS complement(3317..4177) /transl_table=11 /note="ampicillin resistance" /protein_id="SJL88362.1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" misc_feature 4313..4690 /note="hCMV" regulatory 4696..6037 /note="AG" /regulatory_class="promoter" misc_feature 5065..5989 /note="intron" BASE COUNT 1320 a 1566 c 1625 g 1529 t ORIGIN 1 ttcggcacga ggttcttaga cattttatta cgcgaaaaca atctcagtct agagtttcaa 61 aagtctttac aaacattata cagtaaactc atataaacag tgtcacaaaa ggggaggcaa 121 agtagttggt atttagagta atcttacagt ttactaaaaa tgtaataaat gtagtagaca 181 ttgatgcata gtaatttcct gaggattatt tttagccctt ccaccacgtc caacatgatt 241 gaaaagtaac aaatataaaa cattactttt ctatggcatc tacaaaattg aagcagttta 301 cattgatggg tgaaagtctt ccaccagctg gcctttgtat aaaaagggaa atgtgtgtga 361 aatatatgta gcaagacaga tggccctact tgctaggttt gggacagaaa tgcagctttt 421 tggtcagcat tgaggcgaag cagggcacct gcttcttgcc gattgcatcc gggtctttgc 481 agaagtccac gcggcgctga gagacctttc tgttcaccag cgtgagcagc tccgtgaact 541 ccagggaact gccgtacctc gccagcatct cacacaaatc ctgaatgtac caggagccat 601 tcacagtttc tcggtgagag taataccctt ctgcgacaga gtagcacatg aggaagtctg 661 cccctgccgg cagcgtgtac acggacgcag catccacctg ggtcacgttg tccagcttgt 721 ctgtctggtg atccaccatg tccaggggaa ccacgggtac gtcatgctgg ctaccccgac 781 aggcctggat gataaatatc ttgggttttc caaccaggct ctgacacttg tctcctttga 841 acaagccagt caacgtctga atttcaattt tggcgtcgta tgcgtaaacg tggttgcctt 901 ccccatggct caggaagaca cagatgaagc aatcggcatc tatgtggctt gaagtcgaca 961 cctcgtgaat tttgagcagg agttcttctg ctctgaggtc gttaaagcat ttcacttcaa 1021 atcctagatc tgaaaacctg cgagtcaggt tgtctctgtc tgcgttggtg ccccgcctct 1081 ctgggagggt caggtgccaa aagaacctct cgtgattgaa gatgagggca actcctctcc 1141 tcttgtggtc catcttgtac tgctcggctg gatcgaacac ttccctactt ttgtagaagc 1201 catcggtttc tgtcatgttt tactttcacc tgcccgcgag ctcgtgccga attcactcct 1261 caggtgcagg ctgcctatca gaaggtggtg gctggtgtgg ccaatgccct ggctcacaaa 1321 taccactgag atctttttcc ctctgccaaa aattatgggg acatcatgaa gccccttgag 1381 catctgactt ctggctaata aaggaaattt attttcattg caatagtgtg ttggaatttt 1441 ttgtgtctct cactcggaag gacatatggg agggcaaatc atttaaaaca tcagaatgag 1501 tatttggttt agagtttggc aacatatgcc catatgctgg ctgccatgaa caaaggttgg 1561 ctataaagag gtcatcagta tatgaaacag ccccctgctg tccattcctt attccataga 1621 aaagccttga cttgaggtta gatttttttt atattttgtt ttgtgttatt tttttcttta 1681 acatccctaa aattttcctt acatgtttta ctagccagat ttttcctcct ctcctgacta 1741 ctcccagtca tagctgtccc tcttctctta tggagatccc tcgacctgca gcccaagctt 1801 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca 1861 caacatacga gccggaagca taaagtgtaa agcctgggtg cctaatgagt gagctaactc 1921 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagcgg 1981 atccgcatct caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc 2041 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg 2101 cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg 2161 gaggcctagg cttttgcaaa aagctaactt gtttattgca gcttataatg gttacaaata 2221 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg 2281 tttgtccaaa ctcatcaatg tatcttatca tgtctggatc cgctgcatta atgaatcggc 2341 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac 2401 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata 2461 cggttatcca cagaatcagg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa 2521 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg 2581 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa 2641 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc 2701 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac 2761 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac 2821 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg 2881 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt 2941 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa 3001 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct 3061 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga 3121 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg 3181 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct 3241 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt 3301 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc 3361 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg 3421 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag 3481 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt 3541 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag 3601 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt 3661 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca 3721 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg 3781 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat 3841 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta 3901 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca 3961 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct 4021 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat 4081 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa 4141 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt 4201 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa 4261 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctggg tcgacattga 4321 ttattgacta gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg 4381 gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc 4441 cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat 4501 tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat 4561 catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat 4621 gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc 4681 gctattacca tggtcgaggt gagccccacg ttctgcttca ctctccccat ctcccccccc 4741 tccccacccc caattttgta tttatttatt ttttaattat tttgtgcagc gatgggggcg 4801 gggggggggg gggggccccc cccaggcggg gcggggcggg gcgaggggcg gggcggggcg 4861 aggcggaaag gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt tccttttatg 4921 gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc gggagtcgtt 4981 gcgcgctgcc ttccccccgt gccccgctcc gccgccgcct cgcgccgccc gccccggctc 5041 tgactgaccg cgttactccc acaggtgagc gggcgggacg gcccttctcc tccgggctgt 5101 aattagcgct tggtttaatg acggcttgtt tcttttctgt ggctgcgtga aagccttgag 5161 gggctccggg agggcccttt gtgcgggggg agcggctcgg ggggtgcgtg cgtgtgtgtg 5221 tgcgtgggga gcgccgcgtg cggctccgcg ctgcccggcg gctgtgagcg ctgcgggcgc 5281 ggcgcggggc tttgtgcgct ccgcagtgtg cgcgagggga gcgcggccgg gggcggtgcc 5341 ccgcggtgcg gggggggctg cgaggggaac aaaggctgcg tgcggggtgt gtgcgtgggg 5401 gggtgagcag ggggtgtggg cgcgtcggtc gggctgcaac cccccctgca cccccctccc 5461 cgagttgctg agcacggccc ggcttcgggt gcggggctcc gtacggggcg tggcgcgggg 5521 ctcgccgtgc cgggcggggg gtggcggcag gtgggggtgc cgggcggggc ggggccgcct 5581 cgggccgggg agggctcggg ggaaggggcg cggcggcccc cggagcgccg gcggctgtcg 5641 aggcgcggcg agccgcagcc attgcctttt atggtaatcg tgcgagaggg cgcagggact 5701 tcctttgtcc caaatctgtg cggagccgaa atctgggagg cgccgccgca ccccctctag 5761 cgggcgcggg gcgaagcggt gcggcgccgg caggaaggaa atgggcgggg agggccttcg 5821 tgcgtcgccg cgccgccgtc cccttctccc tctccagcct cggggctgtc cgcgggggga 5881 cggctgcctt cgggggggac ggggcagggc ggggttcggc ttctggcgtg tgaccggcgg 5941 ctctagagcc tctgctaacc atgttcatgc cttcttcttt ttcctacagc tcctgggcaa 6001 cgtgctggtt attgtgctgt ctcatcattt tggcaaagaa //