LOCUS LT727405 6040 bp DNA circular SYN 06-FEB-2017
DEFINITION Mammalian expression vector pCAGGS-mCASP-6, complete sequence.
ACCESSION LT727405
VERSION LT727405.1
KEYWORDS .
SOURCE Mammalian expression vector pCAGGS-mCASP-6
ORGANISM Mammalian expression vector pCAGGS-mCASP-6
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 6040)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..6040
/organism="Mammalian expression vector pCAGGS-mCASP-6"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 3823."
/db_xref="taxon:1944885"
misc_feature 12..35
/note="5UTR"
CDS 36..866
/transl_table=11
/note="CASP6"
/protein_id="SJL88359.1"
/translation="MTETDGFYKSREVFDPAEQYKMDHKRRGVALIFNHERFFWHLTL
PERRGTNADRDNLTRRFSDLGFEVKCFNDLRAEELLLKIHEVSTSSHIDADCFICVFL
SHGEGNHVYAYDAKIEIQTLTGLFKGDKCQSLVGKPKIFIIQACRGSQHDVPVVPLDM
VDHQTDKLDNVTQVDAASVYTLPAGADFLMCYSVAEGYYSHRETVNGSWYIQDLCEML
ARYGSSLEFTELLTLVNRKVSQRRVDFCKDPDAIGKKQVPCFASMLTKKLHFCPKPSK
"
misc_feature 867..1240
/note="3UTR"
regulatory 1330..1421
/note="polyA"
/regulatory_class="terminator"
misc_feature 1422..1778
/note="3FR"
regulatory complement(1856..1938)
/note="lac"
/regulatory_class="promoter"
rep_origin 2080..2155
/note="SV40 ori"
regulatory 2186..2260
/note="polyA"
/regulatory_class="terminator"
regulatory complement(2186..2260)
/note="polyA"
/regulatory_class="terminator"
rep_origin complement(2375..2776)
/note="pMB1 ori"
CDS complement(3317..4177)
/transl_table=11
/note="ampicillin resistance"
/protein_id="SJL88360.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
misc_feature 4313..4690
/note="hCMV"
regulatory 4696..6037
/note="AG"
/regulatory_class="promoter"
misc_feature 5065..5989
/note="intron"
BASE COUNT 1343 a 1560 c 1631 g 1506 t
ORIGIN
1 ttcggcacga gctcgcgggc aggtgaaagt aaaacatgac agaaaccgat ggcttctaca
61 aaagtaggga agtgttcgat ccagccgagc agtacaagat ggaccacaag aggagaggag
121 ttgccctcat cttcaatcac gagaggttct tttggcacct gaccctccca gagaggcggg
181 gcaccaacgc agacagagac aacctgactc gcaggttttc agatctagga tttgaagtga
241 aatgctttaa cgacctcaga gcagaagaac tcctgctcaa aattcacgag gtgtcgactt
301 caagccacat agatgccgat tgcttcatct gtgtcttcct gagccatggg gaaggcaacc
361 acgtttacgc atacgacgcc aaaattgaaa ttcagacgtt gactggcttg ttcaaaggag
421 acaagtgtca gagcctggtt ggaaaaccca agatatttat catccaggcc tgtcggggta
481 gccagcatga cgtacccgtg gttcccctgg acatggtgga tcaccagaca gacaagctgg
541 acaacgtgac ccaggtggat gctgcgtccg tgtacacgct gccggcaggg gcagacttcc
601 tcatgtgcta ctctgtcgca gaagggtatt actctcaccg agaaactgtg aatggctcct
661 ggtacattca ggatttgtgt gagatgctgg cgaggtacgg cagttccctg gagttcacgg
721 agctgctcac gctggtgaac agaaaggtct ctcagcgccg cgtggacttc tgcaaagacc
781 cggatgcaat cggcaagaag caggtgccct gcttcgcctc aatgctgacc aaaaagctgc
841 atttctgtcc caaacctagc aagtagggcc atctgtcttg ctacatatat ttcacacaca
901 tttccctttt tatacaaagg ccagctggtg gaagactttc acccatcaat gtaaactgct
961 tcaattttgt agatgccata gaaaagtaat gttttatatt tgttactttt caatcatgtt
1021 ggacgtggtg gaagggctaa aaataatcct caggaaatta ctatgcatca atgtctacta
1081 catttattac atttttagta aactgtaaga ttactctaaa taccaactac tttgcctccc
1141 cttttgtgac actgtttata tgagtttact gtataatgtt tgtaaagact tttgaaactc
1201 tagactgaga ttgttttcgc gtaataaaat gtctaagaac ctcgtgccga attcactcct
1261 caggtgcagg ctgcctatca gaaggtggtg gctggtgtgg ccaatgccct ggctcacaaa
1321 taccactgag atctttttcc ctctgccaaa aattatgggg acatcatgaa gccccttgag
1381 catctgactt ctggctaata aaggaaattt attttcattg caatagtgtg ttggaatttt
1441 ttgtgtctct cactcggaag gacatatggg agggcaaatc atttaaaaca tcagaatgag
1501 tatttggttt agagtttggc aacatatgcc catatgctgg ctgccatgaa caaaggttgg
1561 ctataaagag gtcatcagta tatgaaacag ccccctgctg tccattcctt attccataga
1621 aaagccttga cttgaggtta gatttttttt atattttgtt ttgtgttatt tttttcttta
1681 acatccctaa aattttcctt acatgtttta ctagccagat ttttcctcct ctcctgacta
1741 ctcccagtca tagctgtccc tcttctctta tggagatccc tcgacctgca gcccaagctt
1801 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
1861 caacatacga gccggaagca taaagtgtaa agcctgggtg cctaatgagt gagctaactc
1921 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagcgg
1981 atccgcatct caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc
2041 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg
2101 cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg
2161 gaggcctagg cttttgcaaa aagctaactt gtttattgca gcttataatg gttacaaata
2221 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
2281 tttgtccaaa ctcatcaatg tatcttatca tgtctggatc cgctgcatta atgaatcggc
2341 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
2401 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
2461 cggttatcca cagaatcagg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
2521 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
2581 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
2641 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
2701 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
2761 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
2821 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
2881 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
2941 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa
3001 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
3061 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
3121 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
3181 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
3241 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
3301 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
3361 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
3421 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
3481 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
3541 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
3601 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
3661 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
3721 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
3781 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
3841 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
3901 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
3961 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
4021 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
4081 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
4141 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
4201 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
4261 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctggg tcgacattga
4321 ttattgacta gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg
4381 gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc
4441 cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat
4501 tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat
4561 catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat
4621 gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc
4681 gctattacca tggtcgaggt gagccccacg ttctgcttca ctctccccat ctcccccccc
4741 tccccacccc caattttgta tttatttatt ttttaattat tttgtgcagc gatgggggcg
4801 gggggggggg gggggccccc cccaggcggg gcggggcggg gcgaggggcg gggcggggcg
4861 aggcggaaag gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt tccttttatg
4921 gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc gggagtcgtt
4981 gcgcgctgcc ttccccccgt gccccgctcc gccgccgcct cgcgccgccc gccccggctc
5041 tgactgaccg cgttactccc acaggtgagc gggcgggacg gcccttctcc tccgggctgt
5101 aattagcgct tggtttaatg acggcttgtt tcttttctgt ggctgcgtga aagccttgag
5161 gggctccggg agggcccttt gtgcgggggg agcggctcgg ggggtgcgtg cgtgtgtgtg
5221 tgcgtgggga gcgccgcgtg cggctccgcg ctgcccggcg gctgtgagcg ctgcgggcgc
5281 ggcgcggggc tttgtgcgct ccgcagtgtg cgcgagggga gcgcggccgg gggcggtgcc
5341 ccgcggtgcg gggggggctg cgaggggaac aaaggctgcg tgcggggtgt gtgcgtgggg
5401 gggtgagcag ggggtgtggg cgcgtcggtc gggctgcaac cccccctgca cccccctccc
5461 cgagttgctg agcacggccc ggcttcgggt gcggggctcc gtacggggcg tggcgcgggg
5521 ctcgccgtgc cgggcggggg gtggcggcag gtgggggtgc cgggcggggc ggggccgcct
5581 cgggccgggg agggctcggg ggaaggggcg cggcggcccc cggagcgccg gcggctgtcg
5641 aggcgcggcg agccgcagcc attgcctttt atggtaatcg tgcgagaggg cgcagggact
5701 tcctttgtcc caaatctgtg cggagccgaa atctgggagg cgccgccgca ccccctctag
5761 cgggcgcggg gcgaagcggt gcggcgccgg caggaaggaa atgggcgggg agggccttcg
5821 tgcgtcgccg cgccgccgtc cccttctccc tctccagcct cggggctgtc cgcgggggga
5881 cggctgcctt cgggggggac ggggcagggc ggggttcggc ttctggcgtg tgaccggcgg
5941 ctctagagcc tctgctaacc atgttcatgc cttcttcttt ttcctacagc tcctgggcaa
6001 cgtgctggtt attgtgctgt ctcatcattt tggcaaagaa
//