LOCUS LT727403 5797 bp DNA circular SYN 06-FEB-2017
DEFINITION Mammalian expression vector pCAGGS-mCASP-3, complete sequence.
ACCESSION LT727403
VERSION LT727403.1
KEYWORDS .
SOURCE Mammalian expression vector pCAGGS-mCASP-3
ORGANISM Mammalian expression vector pCAGGS-mCASP-3
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 5797)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..5797
/organism="Mammalian expression vector pCAGGS-mCASP-3"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 3821."
/db_xref="taxon:1944883"
misc_feature 78..144
/note="5' UTR of mouse CASP-3"
CDS 145..978
/transl_table=11
/note="mouse CASP-3"
/protein_id="SJL88355.1"
/translation="MENNKTSVDSKSINNFEVKTIHGSKSVDSGIYLDSSYKMDYPEM
GICIIINNKNFHKSTGMSSRSGTDVDAANLRETFMGLKYQVRNKNDLTREDILELMDS
VSKEDHSKRSSFVCVILSHGDEGVIYGTNGPVELKKLTSFFRGDYCRSLTGKPKLFII
QACRGTELDCGIETDSGTDEEMACQKIPVEADFLYAYSTAPGYYSWRNSKDGSWFIQS
LCSMLKLYAHKLEFMHILTRVNRKVATEFESFSLDSTFHAKKQIPCIVSMLTKELYFY
H"
misc_feature 979..1057
/note="3' UTR mouse CASP-3"
regulatory 1087..1178
/note="3' UTR rabbit beta-globin, incl. polyA"
/regulatory_class="terminator"
misc_feature 1179..1535
/note="3' flanking region rabbit beta-globin"
regulatory complement(1613..1695)
/note="lac promoter"
/regulatory_class="promoter"
rep_origin 1837..1912
/note="SV40 ori"
regulatory 1943..2017
/note="SV40 polyA early"
/regulatory_class="terminator"
regulatory complement(1943..2017)
/note="SV40 polyA late"
/regulatory_class="terminator"
rep_origin complement(2132..2533)
/note="pMB1 ori"
CDS complement(3074..3934)
/transl_table=11
/note="ampicillin resistance"
/protein_id="SJL88356.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
regulatory 4070..4447
/note="hCMV-IE enhancer"
/regulatory_class="promoter"
regulatory 4453..5794
/note="AG promoter"
/regulatory_class="promoter"
misc_feature 4822..5746
/note="intron AG promoter"
BASE COUNT 1286 a 1467 c 1607 g 1437 t
ORIGIN
1 ttcctcgagg aattcactcc tcaggtgcag gctgcctatc agaaggtggt ggctggtgtg
61 ggggaattcg gcacgaggct ggtgggatca aagcgcagtg tcctgcggcg gggagcttgg
121 aacggtacgc taagaaaagt gaccatggag aacaacaaaa cctcagtgga ttcaaaatcc
181 attaataatt ttgaagtaaa gaccatacat gggagcaagt cagtggactc tgggatctat
241 ctggacagta gttacaaaat ggattatcct gaaatgggca tatgcataat aattaataat
301 aagaacttcc ataagagcac tggaatgtca tctcgctctg gtacggatgt ggacgcagcc
361 aacctcagag agacattcat gggcctgaaa taccaagtca ggaataaaaa tgatcttact
421 cgtgaagaca ttttggaatt aatggatagt gtttctaagg aagatcatag caaaaggagc
481 agctttgtgt gtgtgattct aagccatggt gatgaagggg tcatttatgg gacaaatggg
541 cctgttgaac tgaaaaagtt gactagcttc ttcagaggcg actactgccg gagtctgact
601 ggaaagccga aactcttcat cattcaggcc tgccggggta cggagctgga ctgtggcatt
661 gagacagaca gtgggactga tgaggagatg gcttgccaga agataccggt ggaggctgac
721 ttcctgtatg cttactctac agcacctggt tactattcct ggagaaattc aaaggacggg
781 tcgtggttca tccagtccct ttgcagcatg ctgaagctgt acgcgcacaa gctagaattt
841 atgcacattc tcactcgcgt taacaggaag gtggcaacgg aattcgagtc cttctccctg
901 gactccactt tccacgcaaa gaaacagatc ccgtgtattg tgtccatgct cacgaaagaa
961 ctgtactttt atcactagag gaatgattgg gggtgggggg gggcgtgttt ctgttttgtt
1021 atgccaaatg agaaagctgt cagggagact ctcatttcca atgccctggc tcacaaatac
1081 cactgagatc tttttccctc tgccaaaaat tatggggaca tcatgaagcc ccttgagcat
1141 ctgacttctg gctaataaag gaaatttatt ttcattgcaa tagtgtgttg gaattttttg
1201 tgtctctcac tcggaaggac atatgggagg gcaaatcatt taaaacatca gaatgagtat
1261 ttggtttaga gtttggcaac atatgcccat atgctggctg ccatgaacaa aggttggcta
1321 taaagaggtc atcagtatat gaaacagccc cctgctgtcc attccttatt ccatagaaaa
1381 gccttgactt gaggttagat tttttttata ttttgttttg tgttattttt ttctttaaca
1441 tccctaaaat tttccttaca tgttttacta gccagatttt tcctcctctc ctgactactc
1501 ccagtcatag ctgtccctct tctcttatgg agatccctcg acctgcagcc caagcttggc
1561 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
1621 catacgagcc ggaagcataa agtgtaaagc ctgggtgcct aatgagtgag ctaactcaca
1681 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagcggatc
1741 cgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa
1801 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag
1861 aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag
1921 gcctaggctt ttgcaaaaag ctaacttgtt tattgcagct tataatggtt acaaataaag
1981 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
2041 gtccaaactc atcaatgtat cttatcatgt ctggatccgc tgcattaatg aatcggccaa
2101 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
2161 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
2221 ttatccacag aatcagggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
2281 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
2341 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
2401 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
2461 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
2521 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
2581 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
2641 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
2701 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
2761 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
2821 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
2881 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
2941 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
3001 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
3061 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
3121 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
3181 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
3241 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
3301 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
3361 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
3421 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
3481 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
3541 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
3601 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
3661 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
3721 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
3781 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
3841 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
3901 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
3961 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
4021 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgggtcg acattgatta
4081 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
4141 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
4201 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
4261 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
4321 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
4381 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
4441 attaccatgg tcgaggtgag ccccacgttc tgcttcactc tccccatctc ccccccctcc
4501 ccacccccaa ttttgtattt atttattttt taattatttt gtgcagcgat gggggcgggg
4561 gggggggggg ggcccccccc aggcggggcg gggcggggcg aggggcgggg cggggcgagg
4621 cggaaaggtg cggcggcagc caatcagagc ggcgcgctcc gaaagtttcc ttttatggcg
4681 aggcggcggc ggcggcggcc ctataaaaag cgaagcgcgc ggcgggcggg agtcgttgcg
4741 cgctgccttc cccccgtgcc ccgctccgcc gccgcctcgc gccgcccgcc ccggctctga
4801 ctgaccgcgt tactcccaca ggtgagcggg cgggacggcc cttctcctcc gggctgtaat
4861 tagcgcttgg tttaatgacg gcttgtttct tttctgtggc tgcgtgaaag ccttgagggg
4921 ctccgggagg gccctttgtg cggggggagc ggctcggggg gtgcgtgcgt gtgtgtgtgc
4981 gtggggagcg ccgcgtgcgg ctccgcgctg cccggcggct gtgagcgctg cgggcgcggc
5041 gcggggcttt gtgcgctccg cagtgtgcgc gaggggagcg cggccggggg cggtgccccg
5101 cggtgcgggg ggggctgcga ggggaacaaa ggctgcgtgc ggggtgtgtg cgtggggggg
5161 tgagcagggg gtgtgggcgc gtcggtcggg ctgcaacccc ccctgcaccc ccctccccga
5221 gttgctgagc acggcccggc ttcgggtgcg gggctccgta cggggcgtgg cgcggggctc
5281 gccgtgccgg gcggggggtg gcggcaggtg ggggtgccgg gcggggcggg gccgcctcgg
5341 gccggggagg gctcggggga aggggcgcgg cggcccccgg agcgccggcg gctgtcgagg
5401 cgcggcgagc cgcagccatt gccttttatg gtaatcgtgc gagagggcgc agggacttcc
5461 tttgtcccaa atctgtgcgg agccgaaatc tgggaggcgc cgccgcaccc cctctagcgg
5521 gcgcggggcg aagcggtgcg gcgccggcag gaaggaaatg ggcggggagg gccttcgtgc
5581 gtcgccgcgc cgccgtcccc ttctccctct ccagcctcgg ggctgtccgc ggggggacgg
5641 ctgccttcgg gggggacggg gcagggcggg gttcggcttc tggcgtgtga ccggcggctc
5701 tagagcctct gctaaccatg ttcatgcctt cttctttttc ctacagctcc tgggcaacgt
5761 gctggttatt gtgctgtctc atcattttgg caaagaa
//